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  1. Article ; Online: Engineering Isoprenoid Quinone Production in Yeast.

    Kaur, Divjot / Alkhder, Duha / Corre, Christophe / Alberti, Fabrizio

    ACS synthetic biology

    2020  Volume 9, Issue 9, Page(s) 2239–2245

    Abstract: Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also ... ...

    Abstract Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also produced by all domains of life. Here, we report the engineering of a baker's yeast strain,
    MeSH term(s) Benzoquinones/chemistry ; Benzoquinones/metabolism ; Metabolic Engineering/methods ; Phosphorus-Oxygen Lyases/genetics ; Phosphorus-Oxygen Lyases/metabolism ; Saccharomyces cerevisiae/metabolism ; Terpenes/chemistry ; Terpenes/metabolism ; Ubiquinone/genetics ; Ubiquinone/metabolism
    Chemical Substances Benzoquinones ; Terpenes ; ubiquinone 6 (1065-31-2) ; Ubiquinone (1339-63-5) ; quinone (3T006GV98U) ; Phosphorus-Oxygen Lyases (EC 4.6.-)
    Language English
    Publishing date 2020-08-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.0c00081
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Regulatory fine-tuning of mcr-1 increases bacterial fitness and stabilises antibiotic resistance in agricultural settings.

    Ogunlana, Lois / Kaur, Divjot / Shaw, Liam P / Jangir, Pramod / Walsh, Timothy / Uphoff, Stephan / MacLean, R C

    The ISME journal

    2023  Volume 17, Issue 11, Page(s) 2058–2069

    Abstract: Antibiotic resistance tends to carry fitness costs, making it difficult to understand how resistance can be maintained in the absence of continual antibiotic exposure. Here we investigate this problem in the context of mcr-1, a globally disseminated gene ...

    Abstract Antibiotic resistance tends to carry fitness costs, making it difficult to understand how resistance can be maintained in the absence of continual antibiotic exposure. Here we investigate this problem in the context of mcr-1, a globally disseminated gene that confers resistance to colistin, an agricultural antibiotic that is used as a last resort for the treatment of multi-drug resistant infections. Here we show that regulatory evolution has fine-tuned the expression of mcr-1, allowing E. coli to reduce the fitness cost of mcr-1 while simultaneously increasing colistin resistance. Conjugative plasmids have transferred low-cost/high-resistance mcr-1 alleles across an incredible diversity of E. coli strains, further stabilising mcr-1 at the species level. Regulatory mutations were associated with increased mcr-1 stability in pig farms following a ban on the use of colistin as a growth promoter that decreased colistin consumption by 90%. Our study shows how regulatory evolution and plasmid transfer can combine to stabilise resistance and limit the impact of reducing antibiotic consumption.
    MeSH term(s) Animals ; Swine ; Colistin/pharmacology ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Drug Resistance, Bacterial/genetics ; Anti-Bacterial Agents/pharmacology ; Bacteria/genetics ; Plasmids/genetics ; Microbial Sensitivity Tests
    Chemical Substances Colistin (Z67X93HJG1) ; Escherichia coli Proteins ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-09-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2406536-5
    ISSN 1751-7370 ; 1751-7362
    ISSN (online) 1751-7370
    ISSN 1751-7362
    DOI 10.1038/s41396-023-01509-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 7773: Show issues

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  3. Article ; Online: High-throughput functional variant screens via in vivo production of single-stranded DNA.

    Schubert, Max G / Goodman, Daniel B / Wannier, Timothy M / Kaur, Divjot / Farzadfard, Fahim / Lu, Timothy K / Shipman, Seth L / Church, George M

    Proceedings of the National Academy of Sciences of the United States of America

    2021  Volume 118, Issue 18

    Abstract: Creating and characterizing individual genetic variants remains limited in scale, compared to the tremendous variation both existing in nature and envisioned by genome engineers. Here we introduce retron library recombineering (RLR), a methodology for ... ...

    Abstract Creating and characterizing individual genetic variants remains limited in scale, compared to the tremendous variation both existing in nature and envisioned by genome engineers. Here we introduce retron library recombineering (RLR), a methodology for high-throughput functional screens that surpasses the scale and specificity of CRISPR-Cas methods. We use the targeted reverse-transcription activity of retrons to produce single-stranded DNA (ssDNA) in vivo, incorporating edits at >90% efficiency and enabling multiplexed applications. RLR simultaneously introduces many genomic variants, producing pooled and barcoded variant libraries addressable by targeted deep sequencing. We use RLR for pooled phenotyping of synthesized antibiotic resistance alleles, demonstrating quantitative measurement of relative growth rates. We also perform RLR using the sheared genomic DNA of an evolved bacterium, experimentally querying millions of sequences for causal variants, demonstrating that RLR is uniquely suited to utilize large pools of natural variation. Using ssDNA produced in vivo for pooled experiments presents avenues for exploring variation across the genome.
    MeSH term(s) Alleles ; CRISPR-Cas Systems/genetics ; DNA, Single-Stranded/biosynthesis ; DNA, Single-Stranded/genetics ; Drug Resistance, Microbial/genetics ; Escherichia coli/genetics ; Gene Library ; Genetic Engineering ; Genome, Bacterial/genetics ; Genomics ; High-Throughput Nucleotide Sequencing ; High-Throughput Screening Assays ; Saccharomyces cerevisiae/genetics ; Synthetic Biology
    Chemical Substances DNA, Single-Stranded
    Language English
    Publishing date 2021-04-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2018181118
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Circulating CD14

    Stenzel, Ashley E / Abrams, Scott I / Joseph, Janine M / Goode, Ellen L / Tario, Joseph D / Wallace, Paul K / Kaur, Divjot / Adamson, Anna-Kay / Buas, Matthew F / Lugade, Amit A / Laslavic, Angela / Taylor, Sarah E / Orr, Brian / Edwards, Robert P / Elishaev, Esther / Odunsi, Kunle / Mongiovi, Jennifer M / Etter, John Lewis / Winham, Stacey J /
    Kaufmann, Scott H / Modugno, Francesmary / Moysich, Kirsten B

    American journal of reproductive immunology (New York, N.Y. : 1989)

    2020  Volume 85, Issue 3, Page(s) e13343

    Abstract: Problem: Previous studies identified circulating CD14: Method of study: Incident cases of 236 women with EOC were recruited and comprehensive flow cytometry was utilized to assess the frequency of peripheral blood CD33: Results: Patients with ... ...

    Abstract Problem: Previous studies identified circulating CD14
    Method of study: Incident cases of 236 women with EOC were recruited and comprehensive flow cytometry was utilized to assess the frequency of peripheral blood CD33
    Results: Patients with elevated frequencies of circulating CD14
    Conclusion: These findings support the potential clinical relevance of CD14
    MeSH term(s) Aged ; Biomarkers ; Carcinogenesis ; Disease Progression ; Epithelial Cells/pathology ; Female ; HLA-DR Antigens/metabolism ; Humans ; Imides/immunology ; Immune Tolerance ; Lipopolysaccharide Receptors/metabolism ; Middle Aged ; Neoplasm Staging ; Ovarian Neoplasms/diagnosis ; Ovarian Neoplasms/immunology ; Polyphosphates/immunology ; Prognosis
    Chemical Substances Biomarkers ; HLA-DR Antigens ; Imides ; Lipopolysaccharide Receptors ; Polyphosphates ; monoimido-cyclo-triphosphate
    Language English
    Publishing date 2020-09-21
    Publishing country Denmark
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 604542-x
    ISSN 1600-0897 ; 0271-7352 ; 8755-8920 ; 1046-7408
    ISSN (online) 1600-0897
    ISSN 0271-7352 ; 8755-8920 ; 1046-7408
    DOI 10.1111/aji.13343
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1653: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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