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Article ; Online: Prevalence of massively diluted bone marrow cell samples aspirated from patients with myelodysplastic syndromes (MDS) or suspected of MDS: A retrospective analysis of nationwide samples in Japan.

Ogata, Kiyoyuki / Mochimaru, Yuto / Kasai, Nana / Sei, Kazuma / Kawahara, Naoya / Ogata, Mika / Yamamoto, Yumi

2024

Abstract: Bone marrow (BM) examination is a key element in the diagnosis and prognostic grading of myelodysplastic syndromes (MDSs), and obtaining adequate BM cell samples is critical for accurate test results. Massive haemodilution of aspirated BM samples is a ... ...

Abstract	Bone marrow (BM) examination is a key element in the diagnosis and prognostic grading of myelodysplastic syndromes (MDSs), and obtaining adequate BM cell samples is critical for accurate test results. Massive haemodilution of aspirated BM samples is a well-known problem; however, its incidence in patients with MDS has not been well studied. We report the first study to examine the incidence of massive haemodilution in nationwide BM samples aspirated from patients diagnosed with or suspected of MDS in Japan. Among 283 cases available for analysis, BM smears from 92 cases (32.5%) were hypospicular (massively haemodiluted) and, particularly, no BM particles were observed in 52 cases (18.4%). Regarding hypospicular cases, we examined how the doctors in charge interpreted the BM smears of their patients. In only 19 of 92 cases (20.7%), doctors realised that the BM smears were haemodiluted. Furthermore, the BM biopsy, which can help diagnose hypospicular cases, was oftentimes not performed when the haemodilution was overlooked by doctors (not performed in 50 of 73 such cases). These real-world data highlight that not only researchers who are working to improve diagnostic tests but also clinicians who perform and use diagnostic tests must realise this common and potentially critical problem.
Language	English
Publishing date	2024-04-08
Publishing country	England
Document type	Journal Article
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.19447
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Article ; Online: Relationship between ovarian ultrasonographic findings on the seventh post-estrus day and plasma progesterone concentration, nutritional metabolic factors, and pregnancy outcome in dairy cows.

Kawahara, Naoya / Tsuchiya, Yoshiyuki / Endo, Natsumi / Tanaka, Tomomi

The Journal of reproduction and development

2022 Volume 69, Issue 1, Page(s) 41–47

Abstract: To improve the accuracy of ultrasonographic assessment of luteal function, we investigated the relationship between ovarian ultrasonographic findings on Day 7 (Day 1 = ovulation) and plasma progesterone ( ... ...

Abstract	To improve the accuracy of ultrasonographic assessment of luteal function, we investigated the relationship between ovarian ultrasonographic findings on Day 7 (Day 1 = ovulation) and plasma progesterone (P
MeSH term(s)	Animals ; Cattle ; Female ; Pregnancy ; Corpus Luteum/physiology ; Estrus ; Estrus Synchronization ; Insemination, Artificial/veterinary ; Insulin-Like Growth Factor I ; Lactation ; Pregnancy Outcome ; Progesterone ; Ovary
Chemical Substances	Insulin-Like Growth Factor I (67763-96-6) ; Progesterone (4G7DS2Q64Y)
Language	English
Publishing date	2022-12-18
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2039060-9
ISSN	1348-4400 ; 0916-8818
ISSN (online)	1348-4400
ISSN	0916-8818
DOI	10.1262/jrd.2022-110
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Article ; Online: Myeloblasts transition to megakaryoblastic immunophenotypes over time in some patients with myelodysplastic syndromes.

Ogata, Kiyoyuki / Mochimaru, Yuto / Sei, Kazuma / Kawahara, Naoya / Ogata, Mika / Yamamoto, Yumi

PloS one

2023 Volume 18, Issue 9, Page(s) e0291662

Abstract: Objectives: In myelodysplastic syndromes (MDS), neoplastic myeloblast (CD34+CD13+CD33+ cells) numbers often increase over time, leading to secondary acute myeloid leukemia (AML). In recent studies, blasts in some MDS patients have been found to express ... ...

Abstract	Objectives: In myelodysplastic syndromes (MDS), neoplastic myeloblast (CD34+CD13+CD33+ cells) numbers often increase over time, leading to secondary acute myeloid leukemia (AML). In recent studies, blasts in some MDS patients have been found to express a megakaryocyte-lineage molecule, CD41, and such patients show extremely poor prognosis. This is the first study to evaluate whether myeloblasts transition to CD41+ blasts over time and to investigate the detailed immunophenotypic features of CD41+ blasts in MDS. Methods: We performed a retrospective cohort study, in which time-dependent changes in blast immunophenotypes were analyzed using multidimensional flow cytometry (MDF) in 74 patients with MDS and AML (which progressed from MDS). Results: CD41+ blasts (at least 20% of CD34+ blasts expressing CD41) were detected in 12 patients. In five of these 12 patients, blasts were CD41+ from the first MDF analysis. In the other seven patients, myeloblasts (CD34+CD33+CD41- cells) transitioned to megakaryoblasts (CD34+CD41+ cells) over time, which was often accompanied by disease progression (including leukemic transformation). These CD41+ patients were more frequently observed among patients with monosomal and complex karyotypes. CD41+ blasts were negative for the erythroid antigen, CD235a, and positive for CD33 in all cases, but CD33 expression levels were lower in three cases when compared with CD34+CD41- blasts. Among the five CD41+ patients who underwent extensive immunophenotyping, CD41+ blasts all expressed CD61, but two cases had reduced CD42b expression, three had reduced/absent CD13 expression, and three also expressed CD7. Conclusions: Myeloblasts become megakaryoblastic over time in some MDS patients, and examining the megakaryocyte lineage (not only as a diagnostic work-up but also as follow-up) is needed to detect CD41+ MDS. The immunophenotypic features revealed in this study may have diagnostic relevance for CD41+ MDS patients.
MeSH term(s)	Humans ; Granulocyte Precursor Cells ; Immunophenotyping ; Megakaryocyte Progenitor Cells ; Retrospective Studies ; Antigens, CD34 ; Myelodysplastic Syndromes
Chemical Substances	Antigens, CD34
Language	English
Publishing date	2023-09-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0291662
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Article ; Online: Clinical significance of CD41-positive blasts in association with a monosomal karyotype in patients with myelodysplastic syndrome treated with azacitidine.

Ogata, Kiyoyuki / Sei, Kazuma / Kawahara, Naoya / Yamamoto, Yumi

British journal of haematology

2020 Volume 189, Issue 4, Page(s) e144–e147

MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antimetabolites, Antineoplastic/pharmacology ; Antimetabolites, Antineoplastic/therapeutic use ; Azacitidine/pharmacology ; Azacitidine/therapeutic use ; Female ; Humans ; Karyotype ; Male ; Middle Aged ; Myelodysplastic Syndromes/drug therapy ; Myelodysplastic Syndromes/immunology ; Platelet Membrane Glycoprotein IIb/immunology ; Young Adult
Chemical Substances	Antimetabolites, Antineoplastic ; Platelet Membrane Glycoprotein IIb ; Azacitidine (M801H13NRU)
Language	English
Publishing date	2020-03-04
Publishing country	England
Document type	Letter
ZDB-ID	80077-6
ISSN	1365-2141 ; 0007-1048
ISSN (online)	1365-2141
ISSN	0007-1048
DOI	10.1111/bjh.16565
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Article ; Online: Clinical, immunophenotypic, and cytogenetic characteristics of high-grade myelodysplastic syndromes with CD41-positive progenitor cells.

Ogata, Kiyoyuki / Sei, Kazuma / Kawahara, Naoya / Ogata, Mika / Yamamoto, Yumi

Cytometry. Part B, Clinical cytometry

2021 Volume 104, Issue 1, Page(s) 98–107

Abstract: Background: Patients with myelodysplastic syndromes (MDS) with progenitors expressing CD41 (CD41+ MDS) showed a poor prognosis in a previous study but their detailed characteristics remain unclear.: Methods: One hundred thirty-seven subjects at our ... ...

Abstract	Background: Patients with myelodysplastic syndromes (MDS) with progenitors expressing CD41 (CD41+ MDS) showed a poor prognosis in a previous study but their detailed characteristics remain unclear. Methods: One hundred thirty-seven subjects at our institution were diagnosed with excess blasts (EB)-1, EB-2, and acute myeloid leukemia with a low blast count (20%-30%). The immunophenotypes of progenitor cells in their bone marrow (BM) were determined by CD45-gating flow cytometry. A false-positive reaction to CD41 was eliminated by examining the flow cytometry data of lymphocytes and monocytes in addition to progenitors and by examining CD42b in histological sections. The characteristics were compared between CD41+ and CD41- MDS patients. Results: Forty-three patients (31%) were CD41+. Additionally, 91% of the CD41+ MDS patients were very high-risk defined by the Revised International Prognostic Score System, which was higher than in patients with CD41- MDS (p = 0.015). Approximately 60% of the CD41+ MDS patients had a monosomal karyotype and very poor cytogenetics, which was higher than in CD41- MDS patients (p < 0.001). Normal cytogenetics was less common in CD41+ patients (p = 0.0016). Blasts with bleb formation were more abundant in CD41+ MDS patients (p = 0.026). All CD41+ MDS patients were positive for CD13 and were mostly positive for CD33. The frequency of aberrant expression of other antigens on progenitors was similar between CD41+ and CD41- MDS patients. Conclusions: We determined clinical, immunophenotypic, and cytogenetic characteristics of CD41+ MDS patients. Further studies are needed to improve the survival of these patients.
MeSH term(s)	Humans ; Flow Cytometry ; Myelodysplastic Syndromes/diagnosis ; Myelodysplastic Syndromes/genetics ; Myelodysplastic Syndromes/pathology ; Bone Marrow/pathology ; Karyotyping ; Stem Cells/pathology
Language	English
Publishing date	2021-12-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2099657-3
ISSN	1552-4957 ; 1552-4949 ; 0196-4763
ISSN (online)	1552-4957
ISSN	1552-4949 ; 0196-4763
DOI	10.1002/cyto.b.22052
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Article ; Online: Changes in oxidative stress parameters in healthy and diseased Holstein cows during the transition period in Yamagata Prefecture, Japan.

Tsuchiya, Yoshiyuki / Kawahara, Naoya / Kim, Yo-Han / Ichijo, Toshihiro / Sato, Shigeru

The Journal of veterinary medical science

2020 Volume 82, Issue 7, Page(s) 955–961

Abstract: We investigated changes in oxidative stress markers during the transition period in healthy Holstein cows and those with postpartum diseases. Transition control (TC) Holstein cows (n=9) were evaluated for longitudinal changes during the transition period ...

Abstract	We investigated changes in oxidative stress markers during the transition period in healthy Holstein cows and those with postpartum diseases. Transition control (TC) Holstein cows (n=9) were evaluated for longitudinal changes during the transition period and postpartum diseased (PD) cows with ketosis (n=10), abomasal displacement (n=9), and acute mastitis (n=10) were evaluated in comparison to control cows (n=10). In the TC group, blood samples were collected at 2 weeks prepartum and at 1, 2, 4, 6, and 8 weeks postpartum. Milk yield and composition were measured at 2 and 4 weeks postpartum. In the PD group, blood samples were collected at the first day of examination during the 60 days postpartum. Peripheral oxidative stress parameters (malondialdehyde, MDA; potential antioxidant capacity, PAO; and glutathione peroxidase) were measured, and biochemical analyses were performed. In the TC group, MDA increased significantly postpartum and was correlated with milk yield, blood glucose (Glu), free fatty acid (FFA), β-hydroxybutyric acid (BHB), and aspartate aminotransferase. Compared to the control cows, PD cows with ketosis had significantly higher MDA and significantly lower PAO. Moreover, MDA was significantly correlated with Glu, FFA, and BHB. Postpartum increase in MDA might interact with milk yield and Glu, FFA, and BHB in the TC cows, and postpartum diseases, especially ketosis, might signify its increase and interaction with Glu, FFA, and BHB.
MeSH term(s)	Abomasum/pathology ; Animals ; Antioxidants/analysis ; Cattle ; Cattle Diseases/metabolism ; Female ; Glutathione Peroxidase/blood ; Ketosis/veterinary ; Lactation/metabolism ; Malondialdehyde/blood ; Mastitis, Bovine ; Milk/chemistry ; Oxidative Stress/physiology ; Postpartum Period/metabolism ; Pregnancy
Chemical Substances	Antioxidants ; Malondialdehyde (4Y8F71G49Q) ; Glutathione Peroxidase (EC 1.11.1.9)
Language	English
Publishing date	2020-06-08
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.20-0024
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Article ; Online: Herd-level risk factors associated with Leptospira Hardjo infection in dairy herds in the southern Tohoku, Japan.

Miyama, Takeshi / Watanabe, Eiji / Ogata, Yoshimi / Urushiyama, Yoshiro / Kawahara, Naoya / Makita, Kohei

Preventive veterinary medicine

2018 Volume 149, Page(s) 15–20

Abstract: A cross-sectional study was designed to generate information on the herd level prevalence and the risk factors for Leptospira serovar Hardjo (L. Hardjo) in Yamagata, the southern Tohoku, Japan. Bulk tank milk samples from 109 dairy herds were used to ... ...

Abstract	A cross-sectional study was designed to generate information on the herd level prevalence and the risk factors for Leptospira serovar Hardjo (L. Hardjo) in Yamagata, the southern Tohoku, Japan. Bulk tank milk samples from 109 dairy herds were used to test the herd level sero-prevalence of L. Hardjo using a commercial ELISA kit, which detects both L. interrogans serovar Hardjo and L. borgpetersenii serovar Hardjo. A questionnaire survey was conducted at the sampled farms, and univariable and multivariable analyses were performed. Spatial clustering of L. Hardjo at the herd level was examined using spatial scan statistics. Seventy-one herds were found to be positive for L. Hardjo, and the apparent herd prevalence was 65.1% (95% CI: 56.2-74.1%). The risk factors for sero-positivity were larger herd size (p=0.004) and cows with a history of staying in Hokkaido (p <0.001). The spatial scan statistic detected a most likely cluster (relative risk=1.87, log likelihood ratio=9.93, radius=13.70km, p<0.01) in the southern part of the study area where there are large herd sizes and farm density is high. This study revealed that L. Hardjo is prevalent throughout Yamagata, and large scale herd owners introducing cows from Hokkaido in particular should be aware of the risk of infection.
MeSH term(s)	Animals ; Cattle ; Cattle Diseases/epidemiology ; Cattle Diseases/microbiology ; Cross-Sectional Studies ; Dairying ; Enzyme-Linked Immunosorbent Assay/veterinary ; Female ; Japan/epidemiology ; Leptospira/isolation & purification ; Leptospira interrogans/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Leptospirosis/veterinary ; Risk Factors ; Serogroup
Language	English
Publishing date	2018-01-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	43399-8
ISSN	1873-1716 ; 0167-5877
ISSN (online)	1873-1716
ISSN	0167-5877
DOI	10.1016/j.prevetmed.2017.11.008
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Article: Water intoxication in adult cattle.

Kawahara, Naoya / Ofuji, Sosuke / Abe, Sakae / Tanaka, Ai / Uematsu, Masami / Ogata, Yoshimi

The Japanese journal of veterinary research

2016 Volume 64, Issue 2, Page(s) 159–164

Abstract: Water intoxication is a common disorder in calves and is usually characterized by transient hemoglobinuria. In contrast, the condition is very rare in adult cattle, with few reports on naturally occurring cases. In the present report, four female ... ...

Abstract	Water intoxication is a common disorder in calves and is usually characterized by transient hemoglobinuria. In contrast, the condition is very rare in adult cattle, with few reports on naturally occurring cases. In the present report, four female Japanese Black cattle, aged 16-25 months, showed neurological signs when they drank water following a water outage. Hemoglobinuria was not grossly observed, while severe hyponatremia was revealed by laboratory tests. Autopsy indicated cerebral edema with accumulation of serous fluid in expanded Virchow-Robin spaces. These results indicate the possibility of water intoxication associated with cerebral edema due to severe dilutional hyponatremia in adult cattle.
MeSH term(s)	Animals ; Brain Edema/etiology ; Brain Edema/veterinary ; Cattle ; Cattle Diseases/etiology ; Cattle Diseases/pathology ; Fatal Outcome ; Female ; Hyponatremia/etiology ; Hyponatremia/veterinary ; Water Intoxication/complications ; Water Intoxication/veterinary
Language	English
Publishing date	2016-05
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	41543-1
ISSN	0047-1917
ISSN	0047-1917
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Article ; Online: Revising flow cytometric mini-panel for diagnosing low-grade myelodysplastic syndromes: Introducing a parameter quantifying CD33 expression on CD34+ cells.

Ogata, Kiyoyuki / Sei, Kazuma / Saft, Leonie / Kawahara, Naoya / Porta, Matteo G Della / Chapuis, Nicolas / Yamamoto, Yumi

Leukemia research

2018 Volume 71, Page(s) 75–81

Abstract: Diagnosis of myelodysplastic syndromes (MDS) is not straightforward when objective data, such as blast excess and abnormal cytogenetics, are lacking. Expert laboratories use flow cytometry (FCM) to help diagnose MDS. However, most of FCM protocols for ... ...

Abstract	Diagnosis of myelodysplastic syndromes (MDS) is not straightforward when objective data, such as blast excess and abnormal cytogenetics, are lacking. Expert laboratories use flow cytometry (FCM) to help diagnose MDS. However, most of FCM protocols for MDS are complex, requiring a high level of expertise and high cost. We have reported a FCM mini-panel consisting of four FCM parameters (so-called Ogata score), which is simple to conduct and inexpensive. In this paper, to refine this mini-panel, we have introduced a new FCM parameter, which quantifies CD33 expression on CD34+ cells (called Granulocyte/CD34 cell CD33 ratio). Bone marrow cells from MDS without blast excess (low-grade MDS) and controls were stained with CD34, CD45, and CD33 and analyzed for five parameters ("Granulocyte/CD34 cell CD33 ratio" plus four parameters in the Ogata score). By a multivariate logistic regression model, only three parameters, including "Granulocyte/CD34 cell CD33 ratio" had statistically significant power for diagnosing low-grade MDS. Based on the results, we constructed a new scoring system, which showed approximately 50% sensitivity and more than 95% specificity in diagnosing low-grade MDS. Our revised mini-panel is suitable for screening samples suspected for MDS and provides a basis for further improvement in diagnostic FCM protocols for MDS.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Antigens, CD34 ; Biomarkers/analysis ; Bone Marrow Cells/pathology ; Female ; Flow Cytometry/methods ; Granulocytes/pathology ; Humans ; Immunophenotyping/methods ; Male ; Middle Aged ; Myelodysplastic Syndromes/diagnosis ; Sensitivity and Specificity ; Sialic Acid Binding Ig-like Lectin 3/analysis
Chemical Substances	Antigens, CD34 ; Biomarkers ; CD33 protein, human ; Sialic Acid Binding Ig-like Lectin 3
Language	English
Publishing date	2018-07-10
Publishing country	England
Document type	Journal Article
ZDB-ID	752396-8
ISSN	1873-5835 ; 0145-2126
ISSN (online)	1873-5835
ISSN	0145-2126
DOI	10.1016/j.leukres.2018.07.009
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Article ; Online: A comparison of neurosphere differentiation potential of canine bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells.

Chung, Cheng-Shu / Fujita, Naoki / Kawahara, Naoya / Yui, Sho / Nam, Eunryel / Nishimura, Ryohei

The Journal of veterinary medical science

2013 Volume 75, Issue 7, Page(s) 879–886

Abstract: Stem cell transplantation is one of the most promising yet enigmatic treatments for spinal cord injury (SCI), a common problem in dogs. As pre-differentiated mesenchymal stem cells (MSCs) can be expanded and differentiated into neurospheres in vitro, ... ...

Abstract	Stem cell transplantation is one of the most promising yet enigmatic treatments for spinal cord injury (SCI), a common problem in dogs. As pre-differentiated mesenchymal stem cells (MSCs) can be expanded and differentiated into neurospheres in vitro, before being transplanted back, they may prove to be more beneficial for treating SCI. Therefore, we compared the endogenous differentiation potential, including the neuronal cell differentiation, of neurospheres from canine bone marrow MSCs (cBMMSCs) with that of the adipose tissue-derived MSCs (cADMSCs). Nestin-positive neurospheres were generated from MSCs derived from the bone marrow and adipose tissue. Neuronal cells were differentiated from the neurospheres derived from both these tissues. Gene expression analysis revealed that Nestin, βIII-tubulin, NCAM, OCT4 and SOX2 were expressed in MSCs and the corresponding neurospheres. Notably, cBMMSC-derived neuronal cells expressed higher levels of βIII-tubulin. The mRNA expressions of NANOG, Nestin, OCT4 and SOX2 were upregulated in neurospheres derived from both. Immunofluorescence analysis detected the expression of neuronal markers, namely, βIII-tubulin, GFAP, S100, NF200 and MAP2, in differentiated neuron-like cells. Our findings highlight that both cBMMSCs and cADMSCs could be differentiated into neurospheres and neuron-like cells, and therefore, these cells are suitable candidates for cell transplantation. Further, cADMSCs form a more suitable cell source, as larger number of cells could be harvested from cADMSC-derived neurospheres. Future studies employing in vivo transplantation models to investigate the effectiveness of MSCs for treating SCI are warranted.
MeSH term(s)	Adipose Tissue/cytology ; Animals ; Bone Marrow Cells/physiology ; Cell Differentiation/physiology ; DNA Primers/genetics ; Dog Diseases/therapy ; Dogs ; Fluorescent Antibody Technique/veterinary ; Gene Expression Regulation, Developmental/physiology ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stem Cells/physiology ; Nestin/metabolism ; Neural Cell Adhesion Molecules/metabolism ; Neural Stem Cells/physiology ; Octamer Transcription Factor-3/metabolism ; Real-Time Polymerase Chain Reaction/veterinary ; SOXB1 Transcription Factors/metabolism ; Spinal Cord Injuries/therapy ; Spinal Cord Injuries/veterinary ; Statistics, Nonparametric ; Tubulin/metabolism
Chemical Substances	DNA Primers ; Nestin ; Neural Cell Adhesion Molecules ; Octamer Transcription Factor-3 ; SOXB1 Transcription Factors ; Tubulin
Language	English
Publishing date	2013-02-19
Publishing country	Japan
Document type	Comparative Study ; Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.12-0470
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