LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 65

Search options

  1. Article ; Online: Creation and Functional Analysis of Mycolicibacterium smegmatis Recombinant Strains Carrying the Bacillary Cytochromes CYP106A1 and CYP106A2 Genes

    Karpov, M. V. / Nikolaeva, V. M. / Fokina, V. V. / Shutov, A. A. / Kazantsev, A. V. / Strizhov, N. I. / Donova, M. V.

    Appl Biochem Microbiol. 2022 Dec., v. 58, no. 9 p.947-957

    2022  

    Abstract: Mycolicibacterium smegmatis mc²155 has been genetically modified to be used as a platform for the expression of heterologous cytochrome P450 monooxygenases by introducing deletions in the kshB and kstD genes that encode key stages of the steroid nucleus ... ...

    Abstract Mycolicibacterium smegmatis mc²155 has been genetically modified to be used as a platform for the expression of heterologous cytochrome P450 monooxygenases by introducing deletions in the kshB and kstD genes that encode key stages of the steroid nucleus enzymatic degradation. Three sets of genetic constructs have been created for heterologous expression of the genes encoding P450 cytochromes CYP106A1 from Bacillus megaterium DSM319 and CYP106A2 from B. megaterium ATCC13368 in M. smegmatis mc²155 (ΔkshBΔkstD) cells. The recombinant plasmids contained monocistronic expression cassettes of cytochrome genes (pNS31 and pNS32), tricistronic cassettes of cytochrome genes together with cDNA copies of adrenodoxin and adrenodoxin reductase genes of the bovine adrenal cortex (pNS33 and pNS34), or monocistronic gene cassettes of chimeric cytochromes fused with the DNA sequence encoding the CYP116B2 reductase domain from the soil bacterium Rhodococcus sp. NCIMB 9784 (pNS35 and pNS36). The recombinant strains of mycolicibacteria were shown to selectively monohydroxylate androstenedione (AD) under growth conditions. The product was identified as 15β-hydroxyandrostenedione (15β-OH-AD) by mass spectrometry and ¹H and ¹³C NMR spectroscopy. The maximum level of 15β-OH-AD production (17.3 ± 1.5 mg/L) was observed when using the recombinant M. smegmatis mc²155 (ΔkshBΔkstD) (pNS32) strain, which expresses a single cyp106A2 gene from B. megaterium ATCC13368. Host proteins of M. smegmatis mc²155 were shown to be capable of supplying electrons to heterologous cytochromes to support their hydroxylation activity. The results we obtained are of a priority character, expand the understanding of the hydroxylation of steroid compounds by bacterial cytochromes CYP106A1/A2, and are important for the creation of microbial strains for producing valuable hydroxysteroids.
    Keywords Bacillus megaterium ; adrenal cortex ; androstenedione ; cattle ; cytochrome P-450 ; ferredoxin-NADP reductase ; genes ; heterologous gene expression ; hydroxylation ; mass spectrometry ; nuclear magnetic resonance spectroscopy ; nucleotide sequences ; plasmids ; soil bacteria
    Language English
    Dates of publication 2022-12
    Size p. 947-957.
    Publishing place Pleiades Publishing
    Document type Article ; Online
    ZDB-ID 412550-2
    ISSN 1608-3024 ; 0003-6838
    ISSN (online) 1608-3024
    ISSN 0003-6838
    DOI 10.1134/S0003683822090058
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  2. Article ; Online: Peculiarities of measuring ion energy distribution in plasma with a retarding field analyzer.

    Bardakov, V M / Ivanov, S D / Kazantsev, A V / Strokin, N A

    The Review of scientific instruments

    2015  Volume 86, Issue 5, Page(s) 53501

    Abstract: Presented are the results from tailoring the retarding field energy analyzer to measure the degree of charge compensation and regular patterns in the separations of ions of different mass, with the multicomponent ion flow spreading out in the plasma- ... ...

    Abstract Presented are the results from tailoring the retarding field energy analyzer to measure the degree of charge compensation and regular patterns in the separations of ions of different mass, with the multicomponent ion flow spreading out in the plasma-optical mass separator model.
    Language English
    Publishing date 2015-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209865-9
    ISSN 1089-7623 ; 0034-6748
    ISSN (online) 1089-7623
    ISSN 0034-6748
    DOI 10.1063/1.4920998
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article ; Online: Gender features of rheological properties of blood (plasma viscosity, aggregation and deformation of erythrocytes) in patients with chronic generalized periodontitis

    Kazantsev A.V. / Suyetenkov D.Ye. / /Andronoy E.V./ / J Firsova I.V.

    Саратовский научно-медицинский журнал, Vol 10, Iss 1, Pp 56-

    2014  Volume 61

    Abstract: The goal is to study gender features of rheological properties of blood in patients with chronic generalized periodontitis (CGP) from mild to moderate severity. Material and Methods. 80 patients (43.8% male) with CGP aged 42±5 years have been studied. 41 ...

    Abstract The goal is to study gender features of rheological properties of blood in patients with chronic generalized periodontitis (CGP) from mild to moderate severity. Material and Methods. 80 patients (43.8% male) with CGP aged 42±5 years have been studied. 41 patients (43.9% male) experienced mild severity of CGP, and 39 patients (43.6% male) experienced moderate severity. 40 healthy adults (50% male), aged 31±7 years, have been included into the study. Plasma viscosity on shear rate values 300 sec1 to 5 sec1, and rheological features of erythrocytes (aggregation and deformation) have been evaluated. Results. In healthy women and women with mild severity of CGP, plasma viscosity was lower than in the similar group of men. The rheological features of erythrocytes have not gender differences among healthy adults and patients with mild CGP. Increased severity of CGP has been associated with increased plasma viscosity and rheological features of erythrocytes, particularly in men. Conclusion. Plasma viscosity differs in men and women (childbearing age) (healthy subjects and patients with CGP). In women, severity of pathological changes of plasma viscosity and rheological features of erythrocytes have been determined to be lower than in men.
    Keywords aggregation of erythrocytes ; blood viscosity ; chronic generalized periodontitis ; deformation of erythrocytes ; Medicine (General) ; R5-920
    Subject code 610
    Language Russian
    Publishing date 2014-03-01T00:00:00Z
    Publisher Saratov State Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  4. Article: Efficient Handling of Molecular Flexibility in Lattice Energy Minimization of Organic Crystals.

    Kazantsev, A V / Karamertzanis, P G / Adjiman, C S / Pantelides, C C

    Journal of chemical theory and computation

    2011  Volume 7, Issue 6, Page(s) 1998–2016

    Abstract: This paper presents a novel algorithm, CrystalOptimizer, for the minimization of the lattice energy of crystals formed by flexible molecules. The algorithm employs isolated-molecule quantum mechanical (QM) calculations of the intramolecular energy and ... ...

    Abstract This paper presents a novel algorithm, CrystalOptimizer, for the minimization of the lattice energy of crystals formed by flexible molecules. The algorithm employs isolated-molecule quantum mechanical (QM) calculations of the intramolecular energy and conformation-dependent atomic multipoles in the course of the lattice energy minimization. The algorithm eliminates the need to perform QM calculations at each iteration of the minimization by using Local Approximate Models (LAMs), with a minimal impact on accuracy. Additional computational efficiencies are achieved by storing QM-derived components of the lattice energy model in a database and reusing them in subsequent calculations whenever possible. This makes the approach particularly well suited to applications that involve a sequence of lattice energy evaluations, such as crystal structure prediction. The algorithm is capable of handling efficiently complex systems with considerable conformational flexibility. The paper presents examples of the algorithm's application ranging from single-component crystals to cocrystals and salts of flexible molecules with tens of intramolecular degrees of freedom whose optimal values are determined by the interplay of conformational strain and packing forces. For any given molecule, the degree of flexibility to be considered can vary from a few torsional angles to relaxation of the entire set of torsion angles, bond angles, and bond lengths present in the molecule.
    Language English
    Publishing date 2011-06-14
    Publishing country United States
    Document type Journal Article
    ISSN 1549-9618
    ISSN 1549-9618
    DOI 10.1021/ct100597e
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article: [DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION].

    Petrov, A A / Pyshnaya, N S / Lebedev, V N / Kulish, V S / Stovba, L F / Kazantsev, A V / Borisevich, S V

    Zhurnal mikrobiologii, epidemiologii i immunobiologii

    2015  , Issue 6, Page(s) 82–86

    Abstract: Aim: Detection-and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR).: Materials and methods: VEE, Sindbis, West ... ...

    Abstract Aim: Detection-and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR).
    Materials and methods: VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses were studied. Cell culture of chicken fibroblasts, outbred mice and rats, Javanese macaques were used in the experiments. Biological activity determination of the running culture of causative agents used in the experiments was carried out by negative colony method in monolayer cell culture under agar coating. and using intra-cerebral infection of mice. Reagent kits developed in the 48th Central Research Institute and Institute of Analytical Instrument Engineering were used during execution of experiments of VEE virus RNA detection by RT-PCR and rRT-PCR.
    Results: VEE virus was detected in biological samples by various methods. Data from RT-PCR and rRT-PCR are in accordance with the results of virus detection in samples using sensitive animals.
    Conclusion: Use of molecular-diagnostics methods for detection in biological samples of a causative agent of a dangerous infectious disease is important for procuring biological safety of Russian Federation.
    MeSH term(s) Alphavirus Infections/diagnosis ; Alphavirus Infections/virology ; Animals ; Animals, Outbred Strains ; Chickens ; Encephalitis Virus, Japanese/genetics ; Encephalitis Virus, Japanese/isolation & purification ; Encephalitis Virus, Venezuelan Equine/genetics ; Encephalitis Virus, Venezuelan Equine/isolation & purification ; Encephalitis Viruses, Tick-Borne/genetics ; Encephalitis Viruses, Tick-Borne/isolation & purification ; Encephalitis, Japanese/diagnosis ; Encephalitis, Japanese/virology ; Encephalitis, Tick-Borne/diagnosis ; Encephalitis, Tick-Borne/virology ; Encephalomyelitis, Venezuelan Equine/diagnosis ; Encephalomyelitis, Venezuelan Equine/virology ; Fibroblasts/pathology ; Fibroblasts/virology ; Humans ; Macaca mulatta ; Mice ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Rats ; Reagent Kits, Diagnostic/standards ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Sindbis Virus/genetics ; Sindbis Virus/isolation & purification ; West Nile Fever/diagnosis ; West Nile Fever/virology ; West Nile virus/genetics ; West Nile virus/isolation & purification
    Chemical Substances RNA, Viral ; Reagent Kits, Diagnostic
    Language Russian
    Publishing date 2015-11-14
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 218354-7
    ISSN 0372-9311 ; 0049-8726 ; 0372-8714
    ISSN 0372-9311 ; 0049-8726 ; 0372-8714
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article: Identification by modification-interference of purine N-7 and ribose 2'-OH groups critical for catalysis by bacterial ribonuclease P.

    Kazantsev, A V / Pace, N R

    RNA (New York, N.Y.)

    1998  Volume 4, Issue 8, Page(s) 937–947

    Abstract: The RNA subunit of bacterial ribonuclease P is a catalytic RNA that cleaves precursor tRNAs to generate mature tRNA 5' ends. A self-cleaving RNase P RNA-substrate conjugate was used in modification-interference analysis to identify purine N-7 and ribose ... ...

    Abstract The RNA subunit of bacterial ribonuclease P is a catalytic RNA that cleaves precursor tRNAs to generate mature tRNA 5' ends. A self-cleaving RNase P RNA-substrate conjugate was used in modification-interference analysis to identify purine N-7 and ribose 2'-hydroxyl functional groups that are critical to catalysis. We identify six adenine N-7 groups and only one 2'-hydroxyl that, when substituted with 7-deazaadenine or 2'-deoxy analogues, respectively, reduce the RNase P catalytic rate approximately 10-fold at pH 8 and limiting concentration of magnesium. Two sites of low-level interference by phosphorothioate modification were detected in addition to the four sites of strong interference documented previously. These modification-interference results, the absolute phylogenetic conservation of these functional groups in bacterial RNase P RNA, their proximity to the substrate-phosphate in the tertiary structure of the ribozyme-substrate complex, and the importance of some of the sites for binding of catalytic magnesium all implicate these functional groups as components of the RNase P active site. Five of the 7-deazaadenine interferences are suppressed at pH 6, where the hydrolytic step is rate-limiting, or at saturating concentrations of magnesium. We propose, therefore, that these base functional groups are specifically engaged in the catalytic center of RNase P RNA, possibly by involvement in magnesium-dependent folding. One 7-deazaadenine interference and one 2'-deoxy-interference, although partially suppressed at pH 6, are not suppressed at saturating magnesium concentrations. This implicates these groups in magnesium-independent folding of the catalytic substructure of the ribozyme.
    MeSH term(s) Base Sequence ; Binding Sites ; Deoxyribonucleotides/chemistry ; Endoribonucleases/chemistry ; Endoribonucleases/drug effects ; Endoribonucleases/metabolism ; Guanosine/analogs & derivatives ; Guanosine/chemistry ; Hydrogen-Ion Concentration ; Magnesium/pharmacology ; Models, Molecular ; Molecular Sequence Data ; Nuclear Magnetic Resonance, Biomolecular ; Nucleic Acid Conformation ; Purines/chemistry ; RNA Precursors/metabolism ; RNA Processing, Post-Transcriptional ; RNA, Bacterial/chemistry ; RNA, Bacterial/drug effects ; RNA, Bacterial/metabolism ; RNA, Catalytic/chemistry ; RNA, Catalytic/drug effects ; RNA, Catalytic/metabolism ; RNA, Transfer/metabolism ; Ribonuclease P ; Thionucleotides ; Tubercidin/chemistry
    Chemical Substances Deoxyribonucleotides ; Purines ; RNA Precursors ; RNA, Bacterial ; RNA, Catalytic ; Thionucleotides ; Guanosine (12133JR80S) ; 7-deazaguanosine (62160-23-0) ; RNA, Transfer (9014-25-9) ; Endoribonucleases (EC 3.1.-) ; Ribonuclease P (EC 3.1.26.5) ; Magnesium (I38ZP9992A) ; Tubercidin (M351LCX45Y)
    Language English
    Publishing date 1998-08
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1241540-6
    ISSN 1355-8382
    ISSN 1355-8382
    DOI 10.1017/s1355838298980384
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article: [The molecular genetic characteristic of RNA of human enterovirus detected in bio-test from child with serous meningitis].

    Onishchenko, G G / Petrov, A A / Kazantsev, A V / Suroviatkin, A V / Kokonova, M S / Lebedev, V N / Alekseev, Ia I / Varlamov, D A / Kutaev, D A / Vakhnov, E Iu / Borisevich, S V

    Klinicheskaia laboratornaia diagnostika

    2015  Volume 59, Issue 12, Page(s) 44–47

    Abstract: The article considers molecular genetic characteristic of RNA of human enterovirus detected in bio-test from child with serous meningitis. The nucleotide sequence of genome DNA is analyzed. In 98% it is identical to corresponding nucleotide sequences of ... ...

    Abstract The article considers molecular genetic characteristic of RNA of human enterovirus detected in bio-test from child with serous meningitis. The nucleotide sequence of genome DNA is analyzed. In 98% it is identical to corresponding nucleotide sequences of strains of human enterovirus A serotype 71 detected in China.
    MeSH term(s) Base Sequence ; China ; Diagnosis ; Enterovirus A, Human/genetics ; Enterovirus A, Human/isolation & purification ; Enterovirus Infections/diagnosis ; Enterovirus Infections/genetics ; Enterovirus Infections/mortality ; Enterovirus Infections/virology ; Humans ; Meningitis/mortality ; Meningitis/virology ; Phylogeny ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Russia
    Chemical Substances RNA, Viral
    Language Russian
    Publishing date 2015-04-13
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 1155086-7
    ISSN 0869-2084
    ISSN 0869-2084
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article ; Online: WEST NILE FEVER IN THE SARATOV REGION IN 2013-2015.

    Kazorina, E V / Krasovskaya, T Y / Kazantsev, A V / Naydenova, E V / Sharova, I N / Zakharov, K S / Porshakov, A M / Chekashov, V N / Matrosov, A N / Shilov, M M / Yakovlev, S A / Knyazeva, T V / Tolokonnikova, S I / Mironova, N I / Chastov, A A / Kazakova, L V / Kirillova, L P / Krasilnikova, N N / Kozhanova, O I /
    Shcherbakova, S A / Kutyrev, V V

    Voprosy virusologii

    2017  Volume 62, Issue 5, Page(s) 219–226

    Abstract: West Nile virus (WNV) circulation in the territory of Saratov region and its role in the infectious pathology were investigated. For this purpose, in studies conducted in 2013-2015, suspensions of bloodsucking arthropods, organs of birds and small ... ...

    Abstract West Nile virus (WNV) circulation in the territory of Saratov region and its role in the infectious pathology were investigated. For this purpose, in studies conducted in 2013-2015, suspensions of bloodsucking arthropods, organs of birds and small mammals were analyzed for the presence of WNV markers (antigens and/or RNA). The seroprevalence level in live-stock animals and population of the region was evaluated; clinical samples from patients with symptoms compatible with West Nile fever (WNF) were analyzed. As a result of the investigations, WNV markers were detected in field samples gathered in natural biotopes and in the city of Saratov. Immunity to WNV was detected in horses. A stable domain of persons with immunity to this agent was revealed among regional population. Patients with WNF have been annually registered in the region since 2012. The obtained results confirm active circulation of WNF in the Saratov region, as well as formation of stable natural and anthropourgic foci.
    Language English
    Publishing date 2017-10-20
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 201241-8
    ISSN 2411-2097 ; 0507-4088
    ISSN (online) 2411-2097
    ISSN 0507-4088
    DOI 10.18821/0507-4088-2017-62-5-219-226
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article: [Use of autologous progenitor cells of the bone marrow in treatment of patients with lower-limb atherosclerosis obliterans].

    Korymasov, E A / Tiumina, O V / Aiupov, A M / Kazantsev, A V / Rossiev, V A / Mikheev, G V / Volchkov, S E / Toropovskiĭ, A N

    Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery

    2009  Volume 15, Issue 3, Page(s) 28–31

    Abstract: Presented herein are the outcomes of using autologous progenitor cells of the bone marrow in a total offorty-two male patients suffering from atherosclerosis obliterans of the lower-limb arteries with degree II B of ischaemia according to the ... ...

    Abstract Presented herein are the outcomes of using autologous progenitor cells of the bone marrow in a total offorty-two male patients suffering from atherosclerosis obliterans of the lower-limb arteries with degree II B of ischaemia according to the classification of A. V. Pokrovsky, preconditioned by involvement of the femoropopliteal-tibial segment with no possibility to perform a reconstructive operation. It was a randomized, double-blind, placebo-controlled study limited by a clinical approbation of the method concerned. Bone marrow was sampled from the crests of the iffac bone. Exflisate was subjected to double centrifugation to obtain the leukocytic fraction of the bone marrow, immune magnetic separation--for obtaining the CD 133+ cells. The patients were subdivided into three groups, each consisting of 14 subjects. Group One patients received autologous progenitor cells CD133+, Group Two patients were given the leukocytic fraction of the marrow (CD34+), and Group Three patients (comparison group) received normal saline as aplacebo. The preparations were administered into the muscles of the internal and external surface of the crus. The clinical outcomes were evaluated according to the Rutherford scale and demonstrated that Group One and Group Two patients given the cellular material exhibited a statistically significant improvement of their clinical condition, as compared with the findings obtained in the placebo group (P < 0.001, by the Mann-Witney test). Based on the results of the treadmill tests performed after 1, 6 and 12 months, the distance of pain-free walk was noted to statistically significantly increase in Group One and Group Two patients, as compared with those from the placebo group. The proposed method of treatment may be recommended for multicenter clinical trials.
    MeSH term(s) Aged ; Angiography ; Arteriosclerosis Obliterans/diagnostic imaging ; Arteriosclerosis Obliterans/surgery ; Bone Marrow Cells/cytology ; Double-Blind Method ; Follow-Up Studies ; Humans ; Leg/blood supply ; Male ; Middle Aged ; Stem Cell Transplantation/methods ; Transplantation, Autologous ; Treatment Outcome
    Language Russian
    Publishing date 2009
    Publishing country Russia (Federation)
    Document type Comparative Study ; Journal Article ; Randomized Controlled Trial
    ZDB-ID 2186202-3
    ISSN 1027-6661
    ISSN 1027-6661
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article: Photoaffinity cross-linking and RNA structure analysis.

    Thomas, B C / Kazantsev, A V / Chen, J L / Pace, N R

    Methods in enzymology

    2000  Volume 318, Page(s) 136–147

    MeSH term(s) Bacillus subtilis/genetics ; Bacteriophages/enzymology ; Computer Simulation ; Cross-Linking Reagents/pharmacology ; Endoribonucleases/chemistry ; Escherichia coli/genetics ; Escherichia coli Proteins ; Genetic Techniques ; Models, Chemical ; Models, Molecular ; Nucleic Acid Conformation ; Phylogeny ; RNA/chemistry ; RNA/metabolism ; RNA, Catalytic/chemistry ; RNA, Transfer/metabolism ; Ribonuclease P ; Transcription, Genetic ; Ultraviolet Rays
    Chemical Substances Cross-Linking Reagents ; Escherichia coli Proteins ; RNA, Catalytic ; RNA (63231-63-0) ; RNA, Transfer (9014-25-9) ; Endoribonucleases (EC 3.1.-) ; Ribonuclease P (EC 3.1.26.5) ; ribonuclease P, E coli (EC 3.1.26.5)
    Language English
    Publishing date 2000
    Publishing country United States
    Document type Journal Article
    ISSN 0076-6879
    ISSN 0076-6879
    DOI 10.1016/s0076-6879(00)18049-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top