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  1. Article: [Progress and practice of objective measurement of physical behaviors in large-scale cohort research].

    Chen, Y Y / Ke, Y L / Lyu, J / Sun, D J Y / Pan, L / Pei, P / Du, H D / Chen, J S / Chen, Z M / Li, L M / Doherty, D O H E R T Y / Yu, C Q

    Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

    2024  Volume 45, Issue 1, Page(s) 35–40

    Abstract: Due to the limited reliability of traditional self-completed questionnaire, the accuracy of measurement of physical behaviors (physical activity, sedentary behavior and sleep) is not high. With the development of technology, wearable devices (e.g. ... ...

    Abstract Due to the limited reliability of traditional self-completed questionnaire, the accuracy of measurement of physical behaviors (physical activity, sedentary behavior and sleep) is not high. With the development of technology, wearable devices (e.g. accelerometer) can be used for more accurate measurement of physical behaviors and have great application potential in large-scale research. However, the data of objective measurement of physical behaviors from large-scale cohort research in Asian populations is still limited. Between August 2020 and December 2021, the 3
    MeSH term(s) Humans ; Female ; Aged ; Middle Aged ; Male ; Reproducibility of Results ; Accelerometry ; Exercise ; Surveys and Questionnaires ; Sleep
    Language Chinese
    Publishing date 2024-01-17
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 645026-x
    ISSN 0254-6450
    ISSN 0254-6450
    DOI 10.3760/cma.j.cn112338-20230724-00036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Chondroitin Sulfate Enhances Proliferation and Migration via Inducing β-Catenin and Intracellular ROS as Well as Suppressing Metalloproteinases through Akt/NF-κB Pathway Inhibition in Human Chondrocytes.

    Hsu, H-C / Ke, Y-L / Lai, Y-H / Hsieh, W-C / Lin, C-H / Huang, S-S / Peng, J-Y / Chen, C-H

    The journal of nutrition, health & aging

    2022  Volume 26, Issue 3, Page(s) 307–313

    Abstract: Background: Chondroitin sulfate (CS) is found in humans' cartilage, bone, cornea, skin, and arterial wall. It consists of the foundation substance in the extracellular matrix (ECM) of connective tissue. The oral supplement form of CS is clinically used ... ...

    Abstract Background: Chondroitin sulfate (CS) is found in humans' cartilage, bone, cornea, skin, and arterial wall. It consists of the foundation substance in the extracellular matrix (ECM) of connective tissue. The oral supplement form of CS is clinically used in treating osteoarthritis (OA).
    Methods: Cell migration was observed by the transwell assay. The EMT, Akt/IKK/IκB pathways, TIMPs, collagen and MMPs in cell lysate were determined by Western blotting. The expression of MMP activity was determined by gelatin zymography. The production of reactive oxygen species (ROS) was determined by using a fluorescence spectrophotometer.
    Results: In the current report, we demonstrated that CS can increase the cell proliferation and migration of chon-001 chondrocytes. Treatment with CS induced the epithelial-mesenchymal transition and increased the expression of type II collagen and TIMP-1/TIMP2 and inhibited the expressions and activities of metalloproteinase-9 (MMP-9) and metalloproteinase-2 (MMP-2). The phosphorylation of Akt, IκB kinase (IKK), IκB and p65 was decreased by CS. CS treatment resulted in β-catenin production and XAV939, a β-catenin inhibitor, and inhibited the cell proliferation by CS treatment. In addition, also significantly induced intracellular ROS generation. Treatment with antioxidant propyl gallate blocked cell migration induced by CS.
    Conclusion: We demonstrated that CS induced cell proliferation and migration of chondrocytes by inducing β-catenin and enhancing ROS production. Moreover, our studies demonstrated that CS can increase the activity of chondrocytes and help patients with osteoarthritis to restore cartilage function.
    MeSH term(s) Cell Proliferation ; Cells, Cultured ; Chondrocytes/metabolism ; Chondroitin Sulfates/metabolism ; Chondroitin Sulfates/pharmacology ; Humans ; Interleukin-1beta/metabolism ; Matrix Metalloproteinase 2/metabolism ; NF-kappa B/metabolism ; Osteoarthritis/drug therapy ; Proto-Oncogene Proteins c-akt/metabolism ; Reactive Oxygen Species/metabolism ; beta Catenin/metabolism
    Chemical Substances Interleukin-1beta ; NF-kappa B ; Reactive Oxygen Species ; beta Catenin ; Chondroitin Sulfates (9007-28-7) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Matrix Metalloproteinase 2 (EC 3.4.24.24)
    Language English
    Publishing date 2022-03-17
    Publishing country France
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2081921-3
    ISSN 1760-4788 ; 1279-7707
    ISSN (online) 1760-4788
    ISSN 1279-7707
    DOI 10.1007/s12603-022-1752-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Chondroitin Sulfate Enhances Proliferation and Migration via Inducing β-Catenin and Intracellular ROS as Well as Suppressing Metalloproteinases through Akt/NF-ϰB Pathway Inhibition in Human Chondrocytes

    Hsu, H.-C. / Ke, Y.-L. / Lai, Y.-H. / Hsieh, W.-C. / Lin, C.-H. / Huang, S.-S. / Peng, J.-Y. / Chen, Ching-Hsein

    journal of nutrition, health & aging. 2022 Mar., v. 26, no. 3

    2022  

    Abstract: BACKGROUND: Chondroitin sulfate (CS) is found in humans’ cartilage, bone, cornea, skin, and arterial wall. It consists of the foundation substance in the extracellular matrix (ECM) of connective tissue. The oral supplement form of CS is clinically used ... ...

    Abstract BACKGROUND: Chondroitin sulfate (CS) is found in humans’ cartilage, bone, cornea, skin, and arterial wall. It consists of the foundation substance in the extracellular matrix (ECM) of connective tissue. The oral supplement form of CS is clinically used in treating osteoarthritis (OA). METHODS: Cell migration was observed by the transwell assay. The EMT, Akt/IKK/IϰB pathways, TIMPs, collagen and MMPs in cell lysate were determined by Western blotting. The expression of MMP activity was determined by gelatin zymography. The production of reactive oxygen species (ROS) was determined by using a fluorescence spectrophotometer. RESULTS: In the current report, we demonstrated that CS can increase the cell proliferation and migration of chon-001 chondrocytes. Treatment with CS induced the epithelial—mesenchymal transition and increased the expression of type II collagen and TIMP-1/TIMP2 and inhibited the expressions and activities of metalloproteinase-9 (MMP-9) and metalloproteinase-2 (MMP-2). The phosphorylation of Akt, IϰB kinase (IKK), IϰB and p65 was decreased by CS. CS treatment resulted in β-catenin production and XAV939, a β-catenin inhibitor, and inhibited the cell proliferation by CS treatment. In addition, also significantly induced intracellular ROS generation. Treatment with antioxidant propyl gallate blocked cell migration induced by CS. CONCLUSION: We demonstrated that CS induced cell proliferation and migration of chondrocytes by inducing β-catenin and enhancing ROS production. Moreover, our studies demonstrated that CS can increase the activity of chondrocytes and help patients with osteoarthritis to restore cartilage function.
    Keywords cartilage ; cell movement ; cell proliferation ; chondrocytes ; chondroitin sulfate ; collagen ; cornea ; extracellular matrix ; fluorescence emission spectroscopy ; gelatin ; humans ; metalloproteinases ; nutrition ; osteoarthritis ; phosphorylation ; propyl gallate ; reactive oxygen species
    Language English
    Dates of publication 2022-03
    Size p. 307-313.
    Publishing place Springer Paris
    Document type Article
    ZDB-ID 2081921-3
    ISSN 1760-4788 ; 1279-7707
    ISSN (online) 1760-4788
    ISSN 1279-7707
    DOI 10.1007/s12603-022-1752-5
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  4. Article ; Online: Effects of cello-oligosaccharide on intestinal microbiota and epithelial barrier function of weanling pigs.

    Jiao, L F / Ke, Y L / Xiao, K / Song, Z H / Hu, C H / Shi, B

    Journal of animal science

    2015  Volume 93, Issue 3, Page(s) 1157–1164

    Abstract: A total of 144 piglets (Duroc × Landrace × Yorkshire; average initial weight of 6.13 kg weaned at 21 ± 1 d age) were allotted to 4 treatments for 2 wk, each of which had 6 pens with 6 pigs per pen. After the feeding experiment, 6 pigs per treatment were ... ...

    Abstract A total of 144 piglets (Duroc × Landrace × Yorkshire; average initial weight of 6.13 kg weaned at 21 ± 1 d age) were allotted to 4 treatments for 2 wk, each of which had 6 pens with 6 pigs per pen. After the feeding experiment, 6 pigs per treatment were slaughtered to investigate the effects of cello-oligosaccharide (COS) on intestinal microbiota and epithelial barrier function. The COS was added to the basal diet at 0, 1.5, 3.0, and 4.5 g/kg diet at the expense of corn, respectively. Plasma -lactate, diamine oxidase (DAO), and the Ussing chamber technique were used to determine the intestinal barrier function. 16S rRNA-based methods were used for intestinal microbiota analysis. The results showed that incremental levels of COS had no effect ( > 0.05) on growth performance. Incremental levels of COS increased lactobacilli in jejunal and colonic contents ( < 0.05); decreased in jejunal contents ( < 0.05) and and in colonic contents ( < 0.05); reduced plasma DAO (linear, = 0.013, and quadratic, = 0.037); increased jejunal mucosa DAO (linear, = 0.003, and quadratic, = 0.008); decreased fluorescein isothiocyanate dextran 4 kDa flux of jejunum and colon ( < 0.05); and increased transepithelial electrical resistance (TER) in colon ( < 0.05), claudin-1 protein expression in jejunal mucosa (linear, = 0.001, and quadratic, = 0.003), and protein expressions of claudin-1 and zonula occludens-1 (ZO-1) in colonic mucosa linearly ( = 0.001 and = 0.001, respectively) and quadratically ( = 0.001 and = 0.002, respectively). The results indicated that the improved microbial ecosystem in the presence of COS might contribute to improvement in intestinal barrier function and tight junction proteins. Results also showed that the appropriate dietary COS supplementation level was 3.0 g/kg in weaned pig diets under our trial conditions.
    MeSH term(s) Animals ; Cellulose/metabolism ; Colon/microbiology ; Diet/veterinary ; Dietary Supplements ; Escherichia coli/drug effects ; Escherichia coli/isolation & purification ; Female ; Gastrointestinal Microbiome/drug effects ; Intestinal Absorption/drug effects ; Intestinal Absorption/physiology ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/physiology ; Jejunum/microbiology ; Male ; Oligosaccharides/metabolism ; Oligosaccharides/pharmacology ; Streptococcus suis/drug effects ; Streptococcus suis/isolation & purification ; Swine/microbiology ; Swine/physiology ; Tight Junction Proteins/drug effects ; Tight Junction Proteins/physiology
    Chemical Substances Oligosaccharides ; Tight Junction Proteins ; Cellulose (9004-34-6)
    Language English
    Publishing date 2015-03
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390959-1
    ISSN 1525-3163 ; 0021-8812
    ISSN (online) 1525-3163
    ISSN 0021-8812
    DOI 10.2527/jas.2014-8248
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Diosmectite-zinc oxide composite improves intestinal barrier restoration and modulates TGF-β1, ERK1/2, and Akt in piglets after acetic acid challenge.

    Song, Z-H / Ke, Y-L / Xiao, K / Jiao, L-F / Hong, Q-H / Hu, C-H

    Journal of animal science

    2015  Volume 93, Issue 4, Page(s) 1599–1607

    Abstract: The present study evaluated the beneficial effect of diosmectite-zinc oxide composite (DS-ZnO) on improving intestinal barrier restoration in piglets after acetic acid challenge and explored the underlying mechanisms. Twenty-four 35-d-old piglets (Duroc × ...

    Abstract The present study evaluated the beneficial effect of diosmectite-zinc oxide composite (DS-ZnO) on improving intestinal barrier restoration in piglets after acetic acid challenge and explored the underlying mechanisms. Twenty-four 35-d-old piglets (Duroc × Landrace × Yorkshire), with an average weight of 8.1 kg, were allocated to 4 treatment groups. On d 1 of the trial, colitis was induced via intrarectal injection of acetic acid (10 mL of 10% acetic acid [ACA] solution for ACA, DS-ZnO, and mixture of diosmectite [DS] and ZnO [DS+ZnO] groups) and the control group was infused with saline. Twenty-four hours after challenged, piglets were fed with the following diets: 1) control group (basal diet), 2) ACA group (basal diet), 3) DS-ZnO group (basal diet supplemented with DS-ZnO), and 4) DS+ZnO group (mixture of 1.5 g diosmectite [DS]/kg and 500 mg Zn/kg from ZnO [equal amount of DS and ZnO in the DS-ZnO treatment group]). On d 8 of the trial, piglets were sacrificed. The results showed that DS-ZnO supplementation improved (P < 0.05) ADG, ADFI, and transepithelial electrical resistance and decreased (P < 0.05) fecal scores, crypt depth, and fluorescein isothiocyanate-dextran 4 kDa (FD4) influx as compared with ACA group. Moreover, DS-ZnO increased (P < 0.05) occludin, claudin-1, and zonula occluden-1 expressions; reduced (P < 0.05) caspase-9 and caspase-3 activity and Bax expression; and improved (P < 0.05) Bcl2, XIAP, and PCNA expression. Diosmectite-zinc oxide composite supplementation also increased (P < 0.05) TGF-β1 expression and ERK1/2 and Akt activation. These results suggest that DS-ZnO attenuates the acetic acid-induced colitis by improving mucosa barrier restoration, inhibiting apoptosis, and improving intestinal epithelial cells proliferation and modulation of TGF-β1 and ERK1/2 and Akt signaling pathway.
    MeSH term(s) Acetic Acid/administration & dosage ; Acetic Acid/adverse effects ; Acetic Acid/pharmacology ; Animals ; Apoptosis/drug effects ; Apoptosis/physiology ; Caspases/drug effects ; Caspases/physiology ; Colitis/chemically induced ; Colitis/drug therapy ; Colitis/veterinary ; Dietary Supplements ; Disease Models, Animal ; Injections ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/pathology ; Intestinal Mucosa/physiopathology ; MAP Kinase Signaling System/drug effects ; MAP Kinase Signaling System/physiology ; Proto-Oncogene Proteins c-akt/drug effects ; Proto-Oncogene Proteins c-akt/physiology ; Silicates/administration & dosage ; Silicates/pharmacology ; Swine/physiology ; Swine Diseases/chemically induced ; Swine Diseases/drug therapy ; Swine Diseases/physiopathology ; Tight Junction Proteins/drug effects ; Tight Junction Proteins/physiology ; Transforming Growth Factor beta1/drug effects ; Transforming Growth Factor beta1/physiology ; Zinc Oxide/administration & dosage ; Zinc Oxide/pharmacology
    Chemical Substances Silicates ; Tight Junction Proteins ; Transforming Growth Factor beta1 ; Smectite (A3N5ZCN45C) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Caspases (EC 3.4.22.-) ; Acetic Acid (Q40Q9N063P) ; Zinc Oxide (SOI2LOH54Z)
    Language English
    Publishing date 2015-04
    Publishing country United States
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 390959-1
    ISSN 1525-3163 ; 0021-8812
    ISSN (online) 1525-3163
    ISSN 0021-8812
    DOI 10.2527/jas.2014-8580
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress.

    Song, J / Xiao, K / Ke, Y L / Jiao, L F / Hu, C H / Diao, Q Y / Shi, B / Zou, X T

    Poultry science

    2014  Volume 93, Issue 3, Page(s) 581–588

    Abstract: The current study investigated the efficacy of a probiotic mixture on ameliorating heat stress-induced impairment of intestinal microflora, morphology, and barrier integrity in broilers. The probiotic mixture contained Bacillus licheniformis, Bacillus ... ...

    Abstract The current study investigated the efficacy of a probiotic mixture on ameliorating heat stress-induced impairment of intestinal microflora, morphology, and barrier integrity in broilers. The probiotic mixture contained Bacillus licheniformis, Bacillus subtilis, and Lactobacillus plantarum. Three hundred sixty 21-d-old Ross 308 male broilers were allocated in 4 experimental treatments, each of which was replicated 6 times with 15 broilers per replicate. A 2 × 2 factorial design was used in the study, and the main factors were composed of diet (basal diet or addition of 1.5 g/kg of probiotic mixture) and temperature (thermoneutral zone or heat stress). From d 22 to 42, birds were either raised in a thermoneutral zone (22°C) or subjected to cyclic heat stress by exposing them to 33°C for 10 h (from 0800 to 1800) and 22°C from 1800 to 0800. Compared with birds kept in the thermoneutral zone, birds subjected to heat stress had reduced ADG and ADFI; lower viable counts of Lactobacillus and Bifidobacterium and increased viable counts of coliforms and Clostridium in small intestinal contents; shorter jejunal villus height, deeper crypt depth, and lower ratio of villus height to crypt depth; decreased jejunal transepithelial electrical resistance and a higher level of jejunal paracellular permeability of fluorescein isothiocyanate dextran 4 kDa; and downregulated protein levels of occludin and zonula occludens-1 (P < 0.05). Supplemental probiotics increased (P < 0.05) small intestinal Lactobacillus and Bifidobacterium, jejunal villus height, protein level of occludin, and decreased (P < 0.05) feed to gain ratio and small intestinal coliforms. These results indicate that dietary addition of probiotic mixture was effective in partially ameliorating intestinal barrier function. But no temperature × diet interaction was observed in the present study, revealing that the supplemented probiotics had the same effect at both temperatures.
    MeSH term(s) Animal Feed/analysis ; Animals ; Avian Proteins/genetics ; Avian Proteins/metabolism ; Bacillus/chemistry ; Blotting, Western/veterinary ; Chickens/anatomy & histology ; Chickens/growth & development ; Chickens/microbiology ; Chickens/physiology ; Diet/veterinary ; Gene Expression Regulation/drug effects ; Heat-Shock Response/drug effects ; Intestines/anatomy & histology ; Intestines/drug effects ; Intestines/microbiology ; Intestines/physiology ; Lactobacillus plantarum/chemistry ; Male ; Microbiota/drug effects ; Probiotics/administration & dosage ; Probiotics/metabolism ; Temperature ; Tight Junction Proteins/genetics ; Tight Junction Proteins/metabolism
    Chemical Substances Avian Proteins ; Tight Junction Proteins
    Language English
    Publishing date 2014-02-26
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 242586-5
    ISSN 1525-3171 ; 0032-5791
    ISSN (online) 1525-3171
    ISSN 0032-5791
    DOI 10.3382/ps.2013-03455
    Database MEDical Literature Analysis and Retrieval System OnLINE

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