Article ; Online: Ground and excited state interactions of metalloporphyrin PtTMPyP4 with polynucleotides [poly(dG-dC)]2 and [poly(dA-dT)]2.
2016 Volume 15, Issue 8, Page(s) 980–987
Abstract: The ground- and excited-state interactions of Pt(ii) meso-tetrakis(4-N-methylpyridyl)porphyrin (PtTMPyP4) with polynucleotides [poly(dG-dC)]2 and [poly(dA-dT)]2 have been investigated using UV/visible, circular dichroism, and steady-state and time- ... ...
| Abstract | The ground- and excited-state interactions of Pt(ii) meso-tetrakis(4-N-methylpyridyl)porphyrin (PtTMPyP4) with polynucleotides [poly(dG-dC)]2 and [poly(dA-dT)]2 have been investigated using UV/visible, circular dichroism, and steady-state and time-resolved emission spectroscopy. PtTMPyP4 intercalates into [poly(dG-dC)]2 with K∼ 10(6) M(-1). When bound to [poly(dG-dC)]2 in aerated solution there is a six-fold emission enhancement with 18 nm red-shift in emission maximum. Emission lifetimes are biexponential. In the presence of [poly(dA-dT)]2 at least two distinct groove-binding modes are observed, depending on the binding ratio. In [poly(dA-dT)]2 the emission intensity increases by a maximum factor of 17 with no shift in the emission spectrum. Three exponentials were required for lifetime fitting. The lower extent of emission enhancement in the presence of [poly(dG-dC)]2 suggests that a slow electron transfer may take place to guanine, which is significantly less efficient than that previously observed for PtTMPyP4 in the presence of guanosine 5'-monophosphate (GMP). The results are compared to those previously recorded with free base H2TMPyP4. |
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| MeSH term(s) | Circular Dichroism ; DNA/chemistry ; DNA/metabolism ; Guanosine Monophosphate/chemistry ; Metalloporphyrins/chemistry ; Organoplatinum Compounds/chemistry ; Poly dA-dT/chemistry ; Polydeoxyribonucleotides/chemistry ; Quantum Theory ; Spectrophotometry, Ultraviolet |
| Chemical Substances | Metalloporphyrins ; Organoplatinum Compounds ; Polydeoxyribonucleotides ; platinum(II) tetrakis(N-methyl-4-pyridyl)porphyrin ; Poly dA-dT (26966-61-0) ; poly(dC-dG) (29855-95-6) ; Guanosine Monophosphate (85-32-5) ; DNA (9007-49-2) |
| Language | English |
| Publishing date | 2016--04 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2072584-X |
| ISSN | 1474-9092 ; 1474-905X |
| ISSN (online) | 1474-9092 |
| ISSN | 1474-905X |
| DOI | 10.1039/c6pp00105j |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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