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  1. Book ; Thesis: Untersuchungen zur endoskopischen elektronischen Specklemuster-Interferometrie sowie deren Anwendung an biologischen Objekten

    Kemper, Björn

    (Berichte aus der Physik)

    2001  

    Author's details Björn Kemper
    Series title Berichte aus der Physik
    Keywords Endoskopie ; Speckle-Interferometrie
    Subject Speckle-Photographie ; Speckle-Fotografie ; ESPI ; Electronic speckle pattern interferometry ; Videoholografie ; Videoholographie ; Laser-Speckle-Interferometrie ; Medizinische Endoskopie ; Endoskopische Untersuchung ; Diagnostische Endoskopie ; Spiegelung
    Language German
    Size IV, 123 S. : Ill., graph. Darst., 21 cm
    Publisher Shaker
    Publishing place Aachen
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Berlin, Humboldt-Univ., Diss., 2001
    HBZ-ID HT013224445
    ISBN 3-8265-9566-1 ; 978-3-8265-9566-0
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2002 A 2812 order for loan Magazin

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  2. Article ; Online: Quantitative Phase Imaging as Sensitive Screening Method for Nanoparticle-Induced Cytotoxicity Assessment.

    Marzi, Anne / Eder, Kai Moritz / Barroso, Álvaro / Kemper, Björn / Schnekenburger, Jürgen

    Cells

    2024  Volume 13, Issue 8

    Abstract: The assessment of nanoparticle cytotoxicity is challenging due to the lack of customized and standardized guidelines for nanoparticle testing. Nanoparticles, with their unique properties, can interfere with biochemical test methods, so multiple tests are ...

    Abstract The assessment of nanoparticle cytotoxicity is challenging due to the lack of customized and standardized guidelines for nanoparticle testing. Nanoparticles, with their unique properties, can interfere with biochemical test methods, so multiple tests are required to fully assess their cellular effects. For a more reliable and comprehensive assessment, it is therefore imperative to include methods in nanoparticle testing routines that are not affected by particles and allow for the efficient integration of additional molecular techniques into the workflow. Digital holographic microscopy (DHM), an interferometric variant of quantitative phase imaging (QPI), has been demonstrated as a promising method for the label-free assessment of the cytotoxic potential of nanoparticles. Due to minimal interactions with the sample, DHM allows for further downstream analyses. In this study, we investigated the capabilities of DHM in a multimodal approach to assess cytotoxicity by directly comparing DHM-detected effects on the same cell population with two downstream biochemical assays. Therefore, the dry mass increase in RAW 264.7 macrophages and NIH-3T3 fibroblast populations measured by quantitative DHM phase contrast after incubation with poly(alkyl cyanoacrylate) nanoparticles for 24 h was compared to the cytotoxic control digitonin, and cell culture medium control. Viability was then determined using a metabolic activity assay (WST-8). Moreover, to determine cell death, supernatants were analyzed for the release of the enzyme lactate dehydrogenase (LDH assay). In a comparative analysis, in which the average half-maximal effective concentration (EC
    MeSH term(s) Animals ; Mice ; NIH 3T3 Cells ; Nanoparticles/toxicity ; Nanoparticles/chemistry ; RAW 264.7 Cells ; Cell Survival/drug effects ; Holography/methods ; Quantitative Phase Imaging
    Language English
    Publishing date 2024-04-17
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells13080697
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Application of Digital Holographic Microscopy to Analyze Changes in T-Cell Morphology in Response to Bacterial Challenge.

    Vom Werth, Kari Lavinia / Kemper, Björn / Kampmeier, Stefanie / Mellmann, Alexander

    Cells

    2023  Volume 12, Issue 5

    Abstract: Quantitative phase imaging (QPI) is a non-invasive, label-free technique used to detect aberrant cell morphologies caused by disease, thus providing a useful diagnostic approach. Here, we evaluated the potential of QPI to differentiate specific ... ...

    Abstract Quantitative phase imaging (QPI) is a non-invasive, label-free technique used to detect aberrant cell morphologies caused by disease, thus providing a useful diagnostic approach. Here, we evaluated the potential of QPI to differentiate specific morphological changes in human primary T-cells exposed to various bacterial species and strains. Cells were challenged with sterile bacterial determinants, i.e., membrane vesicles or culture supernatants, derived from different Gram-positive and Gram-negative bacteria. Timelapse QPI by digital holographic microscopy (DHM) was applied to capture changes in T-cell morphology over time. After numerical reconstruction and image segmentation, we calculated single cell area, circularity and mean phase contrast. Upon bacterial challenge, T-cells underwent rapid morphological changes such as cell shrinkage, alterations of mean phase contrast and loss of cell integrity. Time course and intensity of this response varied between both different species and strains. The strongest effect was observed for treatment with
    MeSH term(s) Humans ; Microscopy/methods ; Anti-Bacterial Agents ; Staphylococcus aureus ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; T-Lymphocytes
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2023-02-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12050762
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Single capture bright field and off-axis digital holographic microscopy: publisher's note.

    Picazo-Bueno, José Ángel / Barroso, Álvaro / Ketelhut, Steffi / Schnekenburger, Jürgen / Micó, Vicente / Kemper, Björn

    Optics letters

    2023  Volume 48, Issue 13, Page(s) 3615

    Abstract: This publisher's note contains corrections to Opt. Lett.48, 876 (2023)10.1364/OL.478674. ...

    Abstract This publisher's note contains corrections to Opt. Lett.48, 876 (2023)10.1364/OL.478674.
    MeSH term(s) Microscopy ; Holography
    Language English
    Publishing date 2023-06-30
    Publishing country United States
    Document type Journal Article
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.497957
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Book ; Online: Fluidische digitalholographische Zellanalyse (FDHZ)

    Kemper, Björn

    Verbundprojekt "Agescreen" ; Abschlussbericht zum Teilvorhaben ; Berichtszeitraum: 01.04.2010 - 30.09.2013

    2013  

    Title variant Final report on the research project "Fluidic digital holographic cell analysis" within the joint research project "Optical determination of cellular material properties for pharmacological high throughput methods (Agescreen)"
    Author's details Autor: Björn Kemper
    Language German
    Size Online-Ressource (36 S., 2,02 MB), Ill., graph. Darst.
    Publisher Technische Informationsbibliothek u. Universitätsbibliothek ; Univ., Centrum für Biomedizinische Optik und Photonik (CeBOP)
    Publishing place Hannover ; Münster
    Document type Book ; Online
    Note Förderkennzeichen BMBF 13N10937. - Verbund-Nr. 01078588. - Engl. Berichtsbl. u. d. T.: Final report on the research project "Fluidic digital holographic cell analysis" within the joint research project "Optical determination of cellular material properties for pharmacological high throughput methods (Agescreen)" ; Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  6. Article ; Online: Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells.

    Rose, Ralph / Kemper, Björn / Schwab, Albrecht / Schlatter, Eberhard / Edemir, Bayram

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 11930

    Abstract: Aquaporin-2-4 (AQP) are expressed in the principal cells of the renal collecting duct (CD). Beside their role in water transport across membranes, several studies showed that AQPs can influence the migration of cells. It is unknown whether this also ... ...

    Abstract Aquaporin-2-4 (AQP) are expressed in the principal cells of the renal collecting duct (CD). Beside their role in water transport across membranes, several studies showed that AQPs can influence the migration of cells. It is unknown whether this also applies for renal CD cells. Another fact is that the expression of these AQPs is highly modulated by the external osmolality. Here we analyzed the localization of AQP2-4 in primary cultured renal inner medullary CD (IMCD) cells and how osmolality influences the migration behavior of these cells. The primary IMCD cells showed a collective migration behavior and there were no differences in the migration speed between cells cultivated either at 300 or 600 mosmol/kg. Acute increase from 300 to 600 mosmol/kg led to a marked reduction and vice versa an acute decrease from 600 to 300 mosmol/kg to a marked increase in migration speed. Interestingly, none of the analyzed AQPs were localized at the leading edge. While AQP3 disappeared within the first 2-3 rows of cells, AQP4 was enriched at the rear end. Further analysis indicated that migration induced lysosomal degradation of AQP3. This could be prevented by activation of the protein kinase A, inducing localization of AQP3 and AQP2 at the leading edge and increasing the migration speed.
    MeSH term(s) Animals ; Aquaporin 3/genetics ; Aquaporin 3/metabolism ; Aquaporin 4/genetics ; Aquaporin 4/metabolism ; Bucladesine/pharmacology ; Cell Movement/drug effects ; Cell Movement/physiology ; Cell Shape ; Cells, Cultured ; Kidney Medulla/cytology ; Kidney Tubules, Collecting/cytology ; Kidney Tubules, Collecting/drug effects ; Kidney Tubules, Collecting/metabolism ; Microscopy, Fluorescence/methods ; Osmolar Concentration ; Primary Cell Culture ; Rats ; Sodium-Hydrogen Exchanger 1/metabolism ; beta Catenin/metabolism
    Chemical Substances Aquaporin 4 ; Sodium-Hydrogen Exchanger 1 ; beta Catenin ; Aquaporin 3 (158801-98-0) ; Bucladesine (63X7MBT2LQ)
    Language English
    Publishing date 2021-06-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-91369-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Label-Free Digital Holographic Microscopy for In Vitro Cytotoxic Effect Quantification of Organic Nanoparticles.

    Eder, Kai Moritz / Marzi, Anne / Barroso, Álvaro / Ketelhut, Steffi / Kemper, Björn / Schnekenburger, Jürgen

    Cells

    2022  Volume 11, Issue 4

    Abstract: Cytotoxicity quantification of nanoparticles is commonly performed by biochemical assays to evaluate their biocompatibility and safety. We explored quantitative phase imaging (QPI) with digital holographic microscopy (DHM) as a time-resolved in vitro ... ...

    Abstract Cytotoxicity quantification of nanoparticles is commonly performed by biochemical assays to evaluate their biocompatibility and safety. We explored quantitative phase imaging (QPI) with digital holographic microscopy (DHM) as a time-resolved in vitro assay to quantify effects caused by three different types of organic nanoparticles in development for medical use. Label-free proliferation quantification of native cell populations facilitates cytotoxicity testing in biomedical nanotechnology. Therefore, DHM quantitative phase images from measurements on nanomaterial and control agent incubated cells were acquired over 24 h, from which the temporal course of the cellular dry mass was calculated within the observed field of view. The impact of LipImage™ 815 lipidots
    MeSH term(s) Biological Assay ; Cell Line ; Holography/methods ; Microscopy/methods ; Nanoparticles
    Language English
    Publishing date 2022-02-12
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11040644
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Investigating Morphological Changes of T-lymphocytes after Exposure with Bacterial Determinants for Early Detection of Septic Conditions.

    Vom Werth, Kari Lavinia / Wörmann, Theresa / Kemper, Björn / Kümpers, Philipp / Kampmeier, Stefanie / Mellmann, Alexander

    Microorganisms

    2022  Volume 10, Issue 2

    Abstract: Sepsis is a leading cause of morbidity and mortality, annually affecting millions of people worldwide. Immediate treatment initiation is crucial to improve the outcome but despite great progress, early identification of septic patients remains a ... ...

    Abstract Sepsis is a leading cause of morbidity and mortality, annually affecting millions of people worldwide. Immediate treatment initiation is crucial to improve the outcome but despite great progress, early identification of septic patients remains a challenge. Recently, white blood cell morphology was proposed as a new biomarker for sepsis diagnosis. In this proof-of-concept study, we aimed to investigate the effect of different bacteria and their determinants on T-lymphocytes by digital holographic microscopy (DHM). We hypothesize that species- and strain-specific morphological changes occur, which may offer a new approach for early sepsis diagnosis and identification of the causative agent. Jurkat cells as a model system were exposed to different
    Language English
    Publishing date 2022-02-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10020391
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Single capture bright field and off-axis digital holographic microscopy.

    Picazo-Bueno, José Ángel / Barroso, Álvaro / Ketelhut, Steffi / Schnekenburger, Jürgen / Micó, Vicente / Kemper, Björn

    Optics letters

    2022  Volume 48, Issue 4, Page(s) 876–879

    Abstract: We report on a single capture approach for simultaneous incoherent bright field (BF) and laser-based quantitative phase imaging (QPI). Common-path digital holographic microscopy (DHM) is implemented in parallel with BF imaging within the optical path of ... ...

    Abstract We report on a single capture approach for simultaneous incoherent bright field (BF) and laser-based quantitative phase imaging (QPI). Common-path digital holographic microscopy (DHM) is implemented in parallel with BF imaging within the optical path of a commercial optical microscope to achieve spatially multiplexed recording of white light images and digital off-axis holograms, which are subsequently numerically demultiplexed. The performance of the proposed multimodal concept is firstly determined by investigations on microspheres. Then, the application for label-free dual-mode QPI and BF imaging of living pancreatic tumor cells is demonstrated.
    Language English
    Publishing date 2022-11-30
    Publishing country United States
    Document type Journal Article
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.478674
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Durable 3D murine ex vivo retina glaucoma models for optical coherence tomography.

    Barroso, Álvaro / Ketelhut, Steffi / Nettels-Hackert, Gerburg / Heiduschka, Peter / Del Amor, Rocío / Naranjo, Valery / Kemper, Björn / Schnekenburger, Jürgen

    Biomedical optics express

    2023  Volume 14, Issue 9, Page(s) 4421–4438

    Abstract: Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical ... ...

    Abstract Durable and standardized phantoms with optical properties similar to native healthy and disease-like biological tissues are essential tools for the development, performance testing, calibration and comparison of label-free high-resolution optical coherence tomography (HR-OCT) systems. Available phantoms are based on artificial materials and reflect thus only partially ocular properties. To address this limitation, we have performed investigations on the establishment of durable tissue phantoms from ex vivo mouse retina for enhanced reproduction of in vivo structure and complexity. In a proof-of-concept study, we explored the establishment of durable 3D models from dissected mouse eyes that reproduce the properties of normal retina structures and tissue with glaucoma-like layer thickness alterations. We explored different sectioning and preparation procedures for embedding normal and N-methyl-D-aspartate (NMDA)-treated mouse retina in transparent gel matrices and epoxy resins, to generate durable three-dimensional tissue models. Sample quality and reproducibility were quantified by thickness determination of the generated layered structures utilizing computer-assisted segmentation of OCT B-scans that were acquired with a commercial HR-OCT system at a central wavelength of 905 nm and analyzed with custom build software. Our results show that the generated 3D models feature thin biological layers close to current OCT resolution limits and glaucoma-like tissue alterations that are suitable for reliable HR-OCT performance characterization. The comparison of data from resin-embedded tissue with native murine retina in gels demonstrates that by utilization of appropriate preparation protocols, highly stable samples with layered structures equivalent to native tissues can be fabricated. The experimental data demonstrate our concept as a promising approach toward the fabrication of durable biological 3D models suitable for high-resolution OCT system performance characterization supporting the development of optimized instruments for ophthalmology applications.
    Language English
    Publishing date 2023-08-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2572216-5
    ISSN 2156-7085
    ISSN 2156-7085
    DOI 10.1364/BOE.494271
    Database MEDical Literature Analysis and Retrieval System OnLINE

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