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  1. Article ; Online: Comparative analysis of transferrin and IgG N-glycosylation in two human populations.

    Trbojević-Akmačić, Irena / Vučković, Frano / Pribić, Tea / Vilaj, Marija / Černigoj, Urh / Vidič, Jana / Šimunović, Jelena / Kępka, Agnieszka / Kolčić, Ivana / Klarić, Lucija / Novokmet, Mislav / Pučić-Baković, Maja / Rapp, Erdmann / Štrancar, Aleš / Polašek, Ozren / Wilson, James F / Lauc, Gordan

    Communications biology

    2023  Volume 6, Issue 1, Page(s) 312

    Abstract: Human plasma transferrin (Tf) N-glycosylation has been mostly studied as a marker for congenital disorders of glycosylation, alcohol abuse, and hepatocellular carcinoma. However, inter-individual variability of Tf N-glycosylation is not known, mainly due ...

    Abstract Human plasma transferrin (Tf) N-glycosylation has been mostly studied as a marker for congenital disorders of glycosylation, alcohol abuse, and hepatocellular carcinoma. However, inter-individual variability of Tf N-glycosylation is not known, mainly due to technical limitations of Tf isolation in large-scale studies. Here, we present a highly specific robust high-throughput approach for Tf purification from human blood plasma and detailed characterization of Tf N-glycosylation on the level of released glycans by ultra-high-performance liquid chromatography based on hydrophilic interactions and fluorescence detection (HILIC-UHPLC-FLD), exoglycosidase sequencing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). We perform a large-scale comparative study of Tf and immunoglobulin G (IgG) N-glycosylation analysis in two human populations and demonstrate that Tf N-glycosylation is associated with age and sex, along with multiple biochemical and physiological traits. Observed association patterns differ compared to the IgG N-glycome corroborating tissue-specific N-glycosylation and specific N-glycans' role in their distinct physiological functions.
    MeSH term(s) Humans ; Glycosylation ; High-Throughput Screening Assays ; Immunoglobulin G/blood ; Immunoglobulin G/chemistry ; Protein Processing, Post-Translational ; Transferrin/chemistry ; Transferrin/isolation & purification ; Polysaccharides/analysis
    Chemical Substances Immunoglobulin G ; Transferrin ; Polysaccharides
    Language English
    Publishing date 2023-03-23
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-023-04685-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Why Not Use the Immunoglobulin G

    Russell, Alyce C / Kepka, Agnieszka / Trbojević-Akmačić, Irena / Ugrina, Ivo / Song, Manshu / Hui, Jennie / Hunter, Michael / Laws, Simon M / Lauc, Gordan / Wang, Wei

    Omics : a journal of integrative biology

    2019  Volume 23, Issue 12, Page(s) 668–670

    MeSH term(s) Absorptiometry, Photon ; Biomarkers/metabolism ; Female ; Glycosylation ; Humans ; Immunoglobulin G/metabolism ; Male ; Multivariate Analysis ; Polysaccharides/metabolism
    Chemical Substances Biomarkers ; Immunoglobulin G ; Polysaccharides
    Language English
    Publishing date 2019-10-25
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 2030312-9
    ISSN 1557-8100 ; 1536-2310
    ISSN (online) 1557-8100
    ISSN 1536-2310
    DOI 10.1089/omi.2019.0155
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Increased central adiposity is associated with pro-inflammatory immunoglobulin G N-glycans.

    Russell, Alyce C / Kepka, Agnieszka / Trbojević-Akmačić, Irena / Ugrina, Ivo / Song, Manshu / Hui, Jennie / Hunter, Michael / Laws, Simon M / Lauc, Gordan / Wang, Wei

    Immunobiology

    2018  Volume 224, Issue 1, Page(s) 110–115

    Abstract: Background: Increased body fat may be associated with an increased risk of developing an underlying pro-inflammatory state, thus leading to greater risk of developing certain chronic conditions. Immunoglobulin G has the ability to exert both anti- and ... ...

    Abstract Background: Increased body fat may be associated with an increased risk of developing an underlying pro-inflammatory state, thus leading to greater risk of developing certain chronic conditions. Immunoglobulin G has the ability to exert both anti- and pro-inflammatory effects, and the N-glycosylation of the fragment crystallisable portion is involved in mediating this process. Body mass index, a rudimentary yet gold standard indication for body fat, has been shown to be associated with agalactosylated immunoglobulin G N-glycans.
    Aim: We aimed to determine the association between increased body fat and the immunoglobulin G glycosylation features, comparing body mass index to other measures of body fat distribution.
    Methods: We investigated a sample of 637 community-based 45-69 year olds, with mixed phenotypes, residing in Busselton, Western Australia. Body mass index and the waist-to-hip and waist-to-height ratios were calculated using anthropometry, while dual-energy x-ray absorptiometry was performed to gain an accurate measure of total and area specific body fat. Serum immunoglobulin GN-glycans were analysed by ultra-performance liquid chromatography.
    Results: Twenty-two N-glycan peaks were found to be associated with at least one of the fat measures. While the previous association of body mass index to agalactosylated immunoglobulin G was replicated, measures of central adiposity explained the most variation in the immunoglobulin G glycome.
    Conclusion: Central adiposity is associated with an increased pro-inflammatory fraction of immunoglobulin G, suggesting that the android/gynoid ratio or waist-to-height ratio instead be considered when controlling for adiposity in immunoglobulin G glycome biomarker studies.
    MeSH term(s) Absorptiometry, Photon ; Adipose Tissue/diagnostic imaging ; Adiposity/physiology ; Aged ; Anthropometry ; Australia/epidemiology ; Body Mass Index ; Chromatography, Liquid ; Community-Based Participatory Research ; Female ; Glycosylation ; Humans ; Immunoglobulin G/chemistry ; Immunoglobulin G/metabolism ; Inflammation Mediators/chemistry ; Inflammation Mediators/metabolism ; Male ; Middle Aged ; Obesity/epidemiology ; Risk Factors
    Chemical Substances Immunoglobulin G ; Inflammation Mediators
    Language English
    Publishing date 2018-10-19
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 563292-4
    ISSN 1878-3279 ; 0171-2985
    ISSN (online) 1878-3279
    ISSN 0171-2985
    DOI 10.1016/j.imbio.2018.10.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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