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  1. Article ; Online: Efficient supply with carbon dioxide from flue gas during large scale production of microalgae: A novel approach for bioenergy facades.

    Kerner, Martin / Wolff, Thorsten / Brinkmann, Torsten

    Bioresource technology

    2023  Volume 391, Issue Pt A, Page(s) 129917

    Abstract: The efficiency of using enriched ... ...

    Abstract The efficiency of using enriched CO
    MeSH term(s) Carbon Dioxide ; Microalgae ; Chlorella ; Culture Media ; Biomass
    Chemical Substances Carbon Dioxide (142M471B3J) ; Culture Media
    Language English
    Publishing date 2023-10-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2023.129917
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  2. Article: Growth of microalgae adapted to high-light temperature conditions in different types of wastewater.

    Abdel-Shafy, Hussein / Moghazy, Reda / Mansour, Mona / Kerner, Martin

    Water science and technology : a journal of the International Association on Water Pollution Research

    2023  Volume 88, Issue 12, Page(s) 3084–3094

    Abstract: To obtain microalgae strains adapted to wastewater in the Mediterranean region, microalgae present in the Nile River were cultivated at both high-light temperature (HLT) and low-light temperature (LLT) conditions. It was found that the species which ... ...

    Abstract To obtain microalgae strains adapted to wastewater in the Mediterranean region, microalgae present in the Nile River were cultivated at both high-light temperature (HLT) and low-light temperature (LLT) conditions. It was found that the species which became dominant under HLT was chlorophyta of the genus Scenedesmus. In contrast, under LLT, bacillariophyta became dominant. The microalgae strain (Scenedesmus arcuatus) was subsequently isolated and cultivated in different types of primary treated wastewater under HLT and LLT conditions. The different types of primary treated wastewater were black water (BW), grey water (GW), and sewage water (SW). Growth rates reached during the exponential phase at HLT using GW, BW, and SW were between 0.38 and 0.4 (day
    MeSH term(s) Wastewater ; Sewage ; Microalgae ; Temperature ; Biomass ; Scenedesmus ; Water ; Nitrogen/analysis
    Chemical Substances Wastewater ; Sewage ; Water (059QF0KO0R) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2023-12-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 764273-8
    ISSN 1996-9732 ; 0273-1223
    ISSN (online) 1996-9732
    ISSN 0273-1223
    DOI 10.2166/wst.2023.381
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  3. Article ; Online: Impact of green and blue-green light on the growth, pigment concentration, and fatty acid unsaturation in the microalga Monoraphidium braunii.

    Helamieh, Mark / Reich, Marco / Rohne, Philipp / Riebesell, Ulf / Kerner, Martin / Kümmerer, Klaus

    Photochemistry and photobiology

    2023  

    Abstract: The spectral composition of light is an important factor for the metabolism of photosynthetic organisms. Several blue light-regulated metabolic processes have already been identified in the industrially relevant microalga Monoraphidium braunii. However, ... ...

    Abstract The spectral composition of light is an important factor for the metabolism of photosynthetic organisms. Several blue light-regulated metabolic processes have already been identified in the industrially relevant microalga Monoraphidium braunii. However, little is known about the spectral impact on this species' growth, fatty acid (FA), and pigment composition. In this study, M. braunii was cultivated under different light spectra (white light: 400-700 nm, blue light: 400-550 nm, green light: 450-600 nm, and red light: 580-700 nm) at 25°C for 96 h. The growth was monitored daily. Additionally, the FA composition, and pigment concentration was analyzed after 96 h. The highest biomass production was observed upon white light and red light irradiation. However, green light also led to comparably high biomass production, fueling the scientific debate about the contribution of weakly absorbed light wavelengths to microalgal biomass production. All light spectra (white, blue, and green) that comprised blue-green light (450-550 nm) led to a higher degree of FA unsaturation and a greater concentration of all identified pigments than red light. These results further contribute to the growing understanding that blue-green light is an essential trigger for maximized pigment concentration and FA unsaturation in green microalgae.
    Language English
    Publishing date 2023-10-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 123540-0
    ISSN 1751-1097 ; 0031-8655
    ISSN (online) 1751-1097
    ISSN 0031-8655
    DOI 10.1111/php.13873
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Thesis: Die Heiratsbeziehungen und das generative Verhalten einer katholischen Dorfbevölkerung Baden-Württembergs zwischen 1861 und 1900

    Kerner, Martin

    1988  

    Author's details vorgelegt von Martin Kerner
    Size 143 S. : graph. Darst., Kt.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Ulm, Univ., Diss., 1989
    HBZ-ID HT003760907
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    91 D 1175 order for loan Magazin

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  5. Article: Blue‐green light is required for a maximized fatty acid unsaturation and pigment concentration in the microalga Acutodesmus obliquus

    Helamieh, Mark / Reich, Marco / Bory, Sophie / Rohne, Philipp / Riebesell, Ulf / Kerner, Martin / Kümmerer, Klaus

    Lipids. 2022 July, v. 57, no. 4-5

    2022  

    Abstract: Blue‐green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on the particular waveband responsible for this stimulation of FA desaturation and its impact on the pigment composition in microalgae ... ...

    Abstract Blue‐green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on the particular waveband responsible for this stimulation of FA desaturation and its impact on the pigment composition in microalgae remains limited. In this study, Acutodesmus obliquus was cultivated for 96 h at 15°C with different light spectra (380–700 nm, 470–700 nm, 520–700 nm, 600–700 nm, and dark controls). Growth was monitored daily, and qualitative characterization of the microalgal FA composition was achieved via gas chromatography coupled with electron impact ionization mass spectrometry (GC‐EI/MS). Additionally, a quantitative analysis of microalgal pigments was performed using high‐performance liquid chromatography with diode array detection (HPLC‐DAD). Spectra that included wavelengths between 470 and 520 nm led to a significantly higher percentage of the polyunsaturated fatty acids (PUFA) 18:3 and 16:4, compared to all other light conditions. However, no significant differences between the red light cultivations and the heterotrophic dark controls were observed for the FA 18:3 and 16:4. These results indicate, that exclusively the blue‐green light waveband between 470 and 520 nm is responsible for a maximized FA unsaturation in A. obliquus. Furthermore, the growth and production of pigments were impaired if blue‐green light (380–520 nm) was absent in the light spectrum. This knowledge can contribute to achieving a suitable microalgal pigment and FA composition for industrial purposes and must be considered in spectrally selective microalgae cultivation systems.
    Keywords diodes ; fatty acids ; gas chromatography ; high performance liquid chromatography ; mass spectrometry ; microalgae ; quantitative analysis ; red light
    Language English
    Dates of publication 2022-07
    Size p. 221-232.
    Publishing place John Wiley & Sons, Inc.
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 241539-2
    ISSN 1558-9307 ; 0024-4201
    ISSN (online) 1558-9307
    ISSN 0024-4201
    DOI 10.1002/lipd.12343
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  6. Article: Assessment of the biotic and abiotic elimination processes of five micropollutants during cultivation of the green microalgae Acutodesmus obliquus

    Reymann, Theo / Kerner, Martin / Kümmerer, Klaus

    Bioresource technology reports. 2020 Sept., v. 11

    2020  

    Abstract: The elimination by photolysis, microalgae and sorption of five persistent micropollutants Sulfamethoxazole (SMX), Ciprofloxacin (CIP), Quetiapine (QUT), Gabapentin (GAB) and 2-Phenyl-5-benzimidazolesulfonic acid (PBSA) was studied. Microalgae growth was ... ...

    Abstract The elimination by photolysis, microalgae and sorption of five persistent micropollutants Sulfamethoxazole (SMX), Ciprofloxacin (CIP), Quetiapine (QUT), Gabapentin (GAB) and 2-Phenyl-5-benzimidazolesulfonic acid (PBSA) was studied. Microalgae growth was not inhibited by the micropollutants. SMX, QUT, CIP were significantly eliminated during exposure with microalgae, while GAB and PBSA remained unaffected. SMX was eliminated by 43% only under optimal cultivation conditions at an elimination rate of 2.1 μg L⁻¹ h⁻¹. Sorption and photolysis were not involved in the elimination of SMX. QUT was eliminated by 99% with a rate of 4.6 μg L⁻¹ h⁻¹ during microalgae cultivation as a result of photolysis and microalgae activity. Microalgae cultivation produced a 52.8% higher elimination compared photolysis. CIP decreased by 86% at a rate of 1.78 μg L⁻¹ h⁻¹ which was mainly produced by photolysis with an impact of microalgae of 10%. Growth rates of the microalgae and elimination rates of the micropollutants were not correlating. While the underlying processes remained unclear, present study shows that biotic processes coupled to the growth of Acutodesmus obliquus removed most of the micropollutants under study at rates exceeding those of the abiotic removal processes.
    Keywords ciprofloxacin ; microalgae ; photolysis ; pollutants ; sorption ; sulfamethoxazole ; technology
    Language English
    Dates of publication 2020-09
    Publishing place Elsevier Ltd
    Document type Article
    Note NAL-AP-2-clean
    ISSN 2589-014X
    DOI 10.1016/j.biteb.2020.100512
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  7. Article ; Online: Blue-green light is required for a maximized fatty acid unsaturation and pigment concentration in the microalga Acutodesmus obliquus.

    Helamieh, Mark / Reich, Marco / Bory, Sophie / Rohne, Philipp / Riebesell, Ulf / Kerner, Martin / Kümmerer, Klaus

    Lipids

    2022  Volume 57, Issue 4-5, Page(s) 221–232

    Abstract: Blue-green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on the particular waveband responsible for this stimulation of FA desaturation and its impact on the pigment composition in microalgae ... ...

    Abstract Blue-green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on the particular waveband responsible for this stimulation of FA desaturation and its impact on the pigment composition in microalgae remains limited. In this study, Acutodesmus obliquus was cultivated for 96 h at 15°C with different light spectra (380-700 nm, 470-700 nm, 520-700 nm, 600-700 nm, and dark controls). Growth was monitored daily, and qualitative characterization of the microalgal FA composition was achieved via gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS). Additionally, a quantitative analysis of microalgal pigments was performed using high-performance liquid chromatography with diode array detection (HPLC-DAD). Spectra that included wavelengths between 470 and 520 nm led to a significantly higher percentage of the polyunsaturated fatty acids (PUFA) 18:3 and 16:4, compared to all other light conditions. However, no significant differences between the red light cultivations and the heterotrophic dark controls were observed for the FA 18:3 and 16:4. These results indicate, that exclusively the blue-green light waveband between 470 and 520 nm is responsible for a maximized FA unsaturation in A. obliquus. Furthermore, the growth and production of pigments were impaired if blue-green light (380-520 nm) was absent in the light spectrum. This knowledge can contribute to achieving a suitable microalgal pigment and FA composition for industrial purposes and must be considered in spectrally selective microalgae cultivation systems.
    MeSH term(s) Biomass ; Chlorophyceae ; Fatty Acids ; Gas Chromatography-Mass Spectrometry ; Microalgae
    Chemical Substances Fatty Acids
    Language English
    Publishing date 2022-04-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 241539-2
    ISSN 1558-9307 ; 0024-4201
    ISSN (online) 1558-9307
    ISSN 0024-4201
    DOI 10.1002/lipd.12343
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  8. Article: Differences in adaptation to light and temperature extremes of Chlorella sorokiniana strains isolated from a wastewater lagoon

    Krimech, Aafaf / Helamieh, Mark / Wulf, Melina / Krohn, Ines / Riebesell, Ulf / Cherifi, Ouafa / Mandi, Laila / Kerner, Martin

    Bioresource technology. 2022 Apr., v. 350

    2022  

    Abstract: Presently, two Chlorella sorokiniana strains sampled during summer (CS-S) and winter (CS-W) from a maturation pond and isolated by dominance were studied on their behavior on temperature and light extremes in batch experiments. Although both strains ... ...

    Abstract Presently, two Chlorella sorokiniana strains sampled during summer (CS-S) and winter (CS-W) from a maturation pond and isolated by dominance were studied on their behavior on temperature and light extremes in batch experiments. Although both strains showed no differences in their tolerance of temperatures up to 45 °C, the growth rates, pigment contents and fatty acid compositions in response to PAR at 700 and 1,500 µmol m⁻²sec⁻¹ differed. CS-W was less affected by photoinhibition and maintained constantly high growth rates. High radiation resulted in both strains in an equivalent decrease of chlorophyll a and accessory pigments indicating that the latter did not function as a light filter. PUFAS (18:3 and 16:3) increased in CS-W at high radiation by > 60% and decreased in CS-S by 8 %. Results indicate that CS-W is highly favorable for mass cultivation particularly in outdoors, in which diurnal variations of solar radiation occur.
    Keywords Chlorella sorokiniana ; chlorophyll ; fatty acids ; photoinhibition ; solar radiation ; summer ; technology ; temperature ; wastewater ; winter
    Language English
    Dates of publication 2022-04
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2022.126931
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  9. Article ; Online: Impact of green and blue‐green light on the growth, pigment concentration, and fatty acid unsaturation in the microalga Monoraphidium braunii

    Helamieh, Mark / Reich, Marco / Rohne, Philipp / Riebesell, Ulf / Kerner, Martin / Kümmerer, Klaus

    2023  

    Abstract: The spectral composition of light is an important factor for the metabolism of photosynthetic organisms. Several blue light-regulated metabolic processes have already been identified in the industrially relevant microalga Monoraphidium braunii. However, ... ...

    Abstract The spectral composition of light is an important factor for the metabolism of photosynthetic organisms. Several blue light-regulated metabolic processes have already been identified in the industrially relevant microalga Monoraphidium braunii. However, little is known about the spectral impact on this species' growth, fatty acid (FA), and pigment composition. In this study, M. braunii was cultivated under different light spectra (white light: 400–700 nm, blue light: 400–550 nm, green light: 450–600 nm, and red light: 580–700 nm) at 25°C for 96 h. The growth was monitored daily. Additionally, the FA composition, and pigment concentration was analyzed after 96 h. The highest biomass production was observed upon white light and red light irradiation. However, green light also led to comparably high biomass production, fueling the scientific debate about the contribution of weakly absorbed light wavelengths to microalgal biomass production. All light spectra (white, blue, and green) that comprised blue-green light (450–550 nm) led to a higher degree of FA unsaturation and a greater concentration of all identified pigments than red light. These results further contribute to the growing understanding that blue-green light is an essential trigger for maximized pigment concentration and FA unsaturation in green microalgae.
    Subject code 580 ; 535
    Language English
    Publishing date 2023-10-26
    Publisher Wiley
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Growth and fatty acid composition of Acutodesmus obliquus under different light spectra and temperatures

    Helamieh, Mark / Gebhardt, Annkathrin / Reich, Marco / Kuhn, Friedericke / Kerner, Martin / Kümmerer, Klaus

    Lipids. 2021 Sept., v. 56, no. 5

    2021  

    Abstract: The combined impact of temperature and light spectra on the fatty acid (FA) composition in microalgae has been sparsely investigated. The aim of this study was to investigate the interactions of light and temperature on the FA composition in Acutodesmus ... ...

    Abstract The combined impact of temperature and light spectra on the fatty acid (FA) composition in microalgae has been sparsely investigated. The aim of this study was to investigate the interactions of light and temperature on the FA composition in Acutodesmus obliquus. For this purpose, A. obliquus was cultivated with different temperatures (20, 30, and 35°C), as well as broad light spectra (blue, green, and red light). Growth and FA composition were monitored daily. Microalgal FA were extracted, and a qualitative characterization was done by gas chromatography coupled with electron impact ionization mass spectrometry (GC‐EI/MS). Compared to red light, green and blue light caused a higher percentage of the polyunsaturated fatty acids (PUFA) 16:4, 18:3, and 18:4, at all temperatures. The highest total percentage of these PUFA were observed at the lowest cultivation temperature and blue and green light. These data imply that a combination of lower temperatures and blue‐green light (450–550 nm) positively influences the activity of specific FA‐desaturases in A. obliquus. Additionally, a lower 16:1 trans/cis ratio was observed upon green and blue light treatment and lower cultivation temperatures. Remarkably, green light treatment resulted in a comparably high growth under all tested conditions. Therefore, a higher content of green light, compared to blue light might additionally lead to a higher biomass concentration. Microalgae cultivation with low temperatures and green light might therefore result in a suitable FA composition for the food industry and a comparably high biomass production.
    Keywords biomass production ; blue light ; fatty acid composition ; food industry ; gas chromatography ; green light ; mass spectrometry ; microalgae ; red light ; temperature
    Language English
    Dates of publication 2021-09
    Size p. 485-498.
    Publishing place John Wiley & Sons, Inc.
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 241539-2
    ISSN 1558-9307 ; 0024-4201
    ISSN (online) 1558-9307
    ISSN 0024-4201
    DOI 10.1002/lipd.12316
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