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  1. AU="Kesy, Julita"
  2. AU="Tamura, Takao"
  3. AU="Verma, Shyam"
  4. AU="Ishibashi, Masumi"
  5. AU="Morgan, Margaret"
  6. AU="Burkes, Robert M"
  7. AU="Friedrich, Thomas C"
  8. AU="Sheri, Amna"
  9. AU=Antonaci Fabio
  10. AU="Amilien, Virginie"
  11. AU=Bellomo Rinaldo AU=Bellomo Rinaldo
  12. AU="Wada, Jun"
  13. AU="Evan, Gerard"
  14. AU="Natale, Darren A"
  15. AU="Neznanov, Nickolay S"
  16. AU="Richards, Emily D"
  17. AU="Cho, Kyung-Jin"
  18. AU=Hodos Rachel A
  19. AU="Sahin, Kazim"
  20. AU="Bieri, Jan"
  21. AU="Procter-Murphy, R"
  22. AU=Hauguel-Moreau Marie
  23. AU="Cheng, Delfine"
  24. AU="Stephanie C. Pennington"
  25. AU="O'Hanlon, Karen A"
  26. AU="Heide Glaesmer"
  27. AU="Paget-Bailly, Philippe"
  28. AU="Rory J McCrimmon"
  29. AU="Ahdoot, Aaron I."
  30. AU="Neote, Kuldeep S"
  31. AU="Shen, Congcong"
  32. AU="Rahi, Kosar"
  33. AU="Channabasavaiah, Jagadish Puralae"
  34. AU="Anselmi, Maurizio"
  35. AU="Chauhan, D."
  36. AU="Nicoll, Roger A"
  37. AU="Kwon, Young-Sam"
  38. AU="Mihwa Lee"
  39. AU="Yuanting Jin"
  40. AU="Ter Haar, Eva"
  41. AU="Wolin, Dan L"
  42. AU="Zhang, Tenan"
  43. AU="Piedrafita, Lídia"
  44. AU="Nandy, Ananya"
  45. AU="Bansemer, Sven"
  46. AU="Kochetov, O"
  47. AU="Liu, Fen"

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  1. Artikel ; Online: Genes and childhood leukemia.

    Kęsy, Julita / Januszkiewicz-Lewandowska, Danuta

    Postepy higieny i medycyny doswiadczalnej (Online)

    2015  Band 69, Seite(n) 302–308

    Abstract: Leukemia is a heterogeneous hematologic malignancy originating from a multipotent hematopoietic stem cell. It ranks among the commonest cancers in childhood and is characterized by excessive proliferation and differentiation block. The process of ... ...

    Abstract Leukemia is a heterogeneous hematologic malignancy originating from a multipotent hematopoietic stem cell. It ranks among the commonest cancers in childhood and is characterized by excessive proliferation and differentiation block. The process of leukemogenesis is governed by genetic changes at both the cytogenetic and molecular level. According to numerous analyses, a large spectrum of mutations and rearrangements underlying the disease affect essential cellular transduction pathways, genes ensuring proper course of hematopoiesis, oncogenes, tumor suppressors and apoptosis regulators. Common lesions include translocations to T cell receptor (TCR) loci in T-lineage acute lymphoblastic leukemia (T-ALL), mutations of transcription factors regulating B-lineage development and cell maturation in B-lineage acute lymphoblastic leukemia (B-ALL) (PAX5, TCF3, EBF1, etc.), aberrational disruption of genes coding for transcription factors and coactivators in acute myeloid leukemia (AML) (e.g. CBF) or BCR-ABL1 fusion and activation of multiple kinases in chronic myeloid leukemia (CML). These alterations severely impair cell function. Broadening knowledge of the genetic background gives an insight into the pathobiology of a disease and allows for a better understanding of it. An appropriate investigation of genomic events yields diagnostic, prognostic and therapeutic implications. Broadening knowledge of the pathogenesis of leukemia seems to be a promising contribution to precise stratification of patients, reducing the toxicity and adverse effects caused by medical intervention, treatment personalization and introduction of targeted therapy accessible to a wide range of patients.
    Mesh-Begriff(e) Adolescent ; Cell Differentiation/genetics ; Child ; Child, Preschool ; Humans ; Infant ; Infant, Newborn ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics ; Leukemia, Myeloid, Acute/genetics ; Mutation ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics ; Prognosis ; Transcription Factors/genetics
    Chemische Substanzen Transcription Factors
    Sprache Englisch
    Erscheinungsdatum 2015-03-05
    Erscheinungsland Poland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 418865-2
    ISSN 1732-2693 ; 0032-5449
    ISSN (online) 1732-2693
    ISSN 0032-5449
    DOI 10.5604/17322693.1142719
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides.

    Michalak, Paula / Soszynska-Jozwiak, Marta / Biala, Ewa / Moss, Walter N / Kesy, Julita / Szutkowska, Barbara / Lenartowicz, Elzbieta / Kierzek, Ryszard / Kierzek, Elzbieta

    Scientific reports

    2019  Band 9, Heft 1, Seite(n) 3801

    Abstract: Influenza virus causes seasonal epidemics and dangerous pandemic outbreaks. It is a single stranded (-)RNA virus with a segmented genome. Eight segments of genomic viral RNA (vRNA) form the virion, which are then transcribed and replicated in host cells. ...

    Abstract Influenza virus causes seasonal epidemics and dangerous pandemic outbreaks. It is a single stranded (-)RNA virus with a segmented genome. Eight segments of genomic viral RNA (vRNA) form the virion, which are then transcribed and replicated in host cells. The secondary structure of vRNA is an important regulator of virus biology and can be a target for finding new therapeutics. In this paper, the secondary structure of segment 5 vRNA is determined based on chemical mapping data, free energy minimization and structure-sequence conservation analysis for type A influenza. The revealed secondary structure has circular folding with a previously reported panhandle motif and distinct novel domains. Conservations of base pairs is 87% on average with many structural motifs that are highly conserved. Isoenergetic microarray mapping was used to additionally validate secondary structure and to discover regions that easy bind short oligonucleotides. Antisense oligonucleotides, which were designed based on modeled secondary structure and microarray mapping, inhibit influenza A virus proliferation in MDCK cells. The most potent oligonucleotides lowered virus titer by ~90%. These results define universal for type A structured regions that could be important for virus function, as well as new targets for antisense therapeutics.
    Mesh-Begriff(e) Genome, Viral ; Influenza A virus/genetics ; Oligonucleotides, Antisense ; Protein Structure, Secondary
    Chemische Substanzen Oligonucleotides, Antisense
    Sprache Englisch
    Erscheinungsdatum 2019-03-07
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-019-40443-7
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Influenza virus segment 5 (+)RNA - secondary structure and new targets for antiviral strategies.

    Soszynska-Jozwiak, Marta / Michalak, Paula / Moss, Walter N / Kierzek, Ryszard / Kesy, Julita / Kierzek, Elzbieta

    Scientific reports

    2017  Band 7, Heft 1, Seite(n) 15041

    Abstract: Influenza A virus is a threat for humans due to seasonal epidemics and occasional pandemics. This virus can generate new strains that are dangerous through nucleotide/amino acid changes or through segmental recombination of the viral RNA genome. It is ... ...

    Abstract Influenza A virus is a threat for humans due to seasonal epidemics and occasional pandemics. This virus can generate new strains that are dangerous through nucleotide/amino acid changes or through segmental recombination of the viral RNA genome. It is important to gain wider knowledge about influenza virus RNA to create new strategies for drugs that will inhibit its spread. Here, we present the experimentally determined secondary structure of the influenza segment 5 (+)RNA. Two RNAs were studied: the full-length segment 5 (+)RNA and a shorter construct containing only the coding region. Chemical mapping data combined with thermodynamic energy minimization were used in secondary structure prediction. Sequence/structure analysis showed that the determined secondary structure of segment 5 (+)RNA is mostly conserved between influenza virus type A strains. Microarray mapping and RNase H cleavage identified accessible sites for oligonucleotides in the revealed secondary structure of segment 5 (+)RNA. Antisense oligonucleotides were designed based on the secondary structure model and tested against influenza virus in cell culture. Inhibition of influenza virus proliferation was noticed, identifying good targets for antisense strategies. Effective target sites fall within two domains, which are conserved in sequence/structure indicating their importance to the virus.
    Mesh-Begriff(e) Animals ; Antiviral Agents/therapeutic use ; Base Sequence ; Dogs ; Humans ; Influenza A Virus, H5N1 Subtype/drug effects ; Influenza A Virus, H5N1 Subtype/genetics ; Influenza, Human/prevention & control ; Influenza, Human/virology ; Madin Darby Canine Kidney Cells ; Models, Molecular ; Nucleic Acid Conformation ; Oligonucleotides, Antisense/genetics ; Open Reading Frames/genetics ; RNA, Viral/chemistry ; RNA, Viral/genetics ; RNA, Viral/metabolism ; Ribonuclease H/metabolism ; Virus Replication/drug effects ; Virus Replication/genetics
    Chemische Substanzen Antiviral Agents ; Oligonucleotides, Antisense ; RNA, Viral ; Ribonuclease H (EC 3.1.26.4)
    Sprache Englisch
    Erscheinungsdatum 2017-11-08
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-15317-5
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus.

    Lenartowicz, Elzbieta / Kesy, Julita / Ruszkowska, Agnieszka / Soszynska-Jozwiak, Marta / Michalak, Paula / Moss, Walter N / Turner, Douglas H / Kierzek, Ryszard / Kierzek, Elzbieta

    PloS one

    2016  Band 11, Heft 2, Seite(n) e0148281

    Abstract: Influenza A is a negative sense RNA virus that kills hundreds of thousands of humans each year. Base pairing in RNA is very favorable, but possibilities for RNA secondary structure of the influenza genomic RNA have not been investigated. This work ... ...

    Abstract Influenza A is a negative sense RNA virus that kills hundreds of thousands of humans each year. Base pairing in RNA is very favorable, but possibilities for RNA secondary structure of the influenza genomic RNA have not been investigated. This work presents the first experimentally-derived exploration of potential secondary structure in an influenza A naked (protein-free) genomic segment. Favorable folding regions are revealed by in vitro chemical structure mapping, thermodynamics, bioinformatics, and binding to isoenergetic microarrays of an entire natural sequence of the 875 nt segment 8 vRNA and of a smaller fragment. Segment 8 has thermodynamically stable and evolutionarily conserved RNA structure and encodes essential viral proteins NEP and NS1. This suggests that vRNA self-folding may generate helixes and loops that are important at one or more stages of the influenza life cycle.
    Mesh-Begriff(e) Base Pairing ; Base Sequence ; Computer Simulation ; Genome, Viral/genetics ; Influenza A Virus, H5N1 Subtype/genetics ; Molecular Sequence Data ; RNA Folding ; RNA, Viral/chemistry ; RNA, Viral/genetics
    Chemische Substanzen RNA, Viral
    Sprache Englisch
    Erscheinungsdatum 2016-02-05
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0148281
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: A Short Chemically Modified dsRNA-Binding PNA (dbPNA) Inhibits Influenza Viral Replication by Targeting Viral RNA Panhandle Structure.

    Kesy, Julita / Patil, Kiran M / Kumar, Subaschandrabose Rajesh / Shu, Zhiyu / Yong, Hui Yee / Zimmermann, Louis / Ong, Alan Ann Lerk / Toh, Desiree-Faye Kaixin / Krishna, Manchugondanahalli S / Yang, Lixia / Decout, Jean-Luc / Luo, Dahai / Prabakaran, Mookkan / Chen, Gang / Kierzek, Elzbieta

    Bioconjugate chemistry

    2019  Band 30, Heft 3, Seite(n) 931–943

    Abstract: RNAs play critical roles in diverse catalytic and regulatory biological processes and are emerging as important disease biomarkers and therapeutic targets. Thus, developing chemical compounds for targeting any desired RNA structures has great potential ... ...

    Abstract RNAs play critical roles in diverse catalytic and regulatory biological processes and are emerging as important disease biomarkers and therapeutic targets. Thus, developing chemical compounds for targeting any desired RNA structures has great potential in biomedical applications. The viral and cellular RNA sequence and structure databases lay the groundwork for developing RNA-binding chemical ligands through the recognition of both RNA sequence and RNA structure. Influenza A virion consists of eight segments of negative-strand viral RNA (vRNA), all of which contain a highly conserved panhandle duplex structure formed between the first 13 nucleotides at the 5' end and the last 12 nucleotides at the 3' end. Here, we report our binding and cell culture anti-influenza assays of a short 10-mer chemically modified double-stranded RNA (dsRNA)-binding peptide nucleic acid (PNA) designed to bind to the panhandle duplex structure through novel major-groove PNA·RNA
    Mesh-Begriff(e) Animals ; Circular Dichroism ; Dogs ; Madin Darby Canine Kidney Cells ; Native Polyacrylamide Gel Electrophoresis ; Nucleic Acid Conformation ; Orthomyxoviridae/drug effects ; Orthomyxoviridae/genetics ; Orthomyxoviridae/physiology ; Peptide Nucleic Acids/chemistry ; Peptide Nucleic Acids/pharmacology ; RNA, Double-Stranded/chemistry ; RNA, Double-Stranded/metabolism ; RNA, Viral/drug effects ; Virus Replication/drug effects
    Chemische Substanzen Peptide Nucleic Acids ; RNA, Double-Stranded ; RNA, Viral
    Sprache Englisch
    Erscheinungsdatum 2019-02-15
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1024041-x
    ISSN 1520-4812 ; 1043-1802
    ISSN (online) 1520-4812
    ISSN 1043-1802
    DOI 10.1021/acs.bioconjchem.9b00039
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 3400: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
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