LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 50

Search options

  1. Article ; Online: Glomerular basement membrane thickness among the Saudi population.

    Kfoury, Hala

    Ultrastructural pathology

    2016  Volume 40, Issue 5, Page(s) 261–264

    Abstract: The aim of this work was to determine the mean glomerular basement membrane (GBM) thickness in the Saudi population. We calculated the average GBM thickness in patients diagnosed with minimal change disease, and the ultrastructural analysis of at least ... ...

    Abstract The aim of this work was to determine the mean glomerular basement membrane (GBM) thickness in the Saudi population. We calculated the average GBM thickness in patients diagnosed with minimal change disease, and the ultrastructural analysis of at least three glomeruli was reviewed using a digital camera installed in an electron microscope. There were a total of 53 cases from 53 Saudi patients aged 2-70 years old. The mean GBM thickness for all cases was 323.6 ± 49.5 nm. There was no significant statistical difference in the mean GBM thickness between males and females. There were significant differences in the mean GBM thickness between all age groups, except for between the age groups 18-60 and >60 years old, where GBM thickness did not differ significantly. Age was significantly correlated with definite progression or diminution in the thickness of the GBM. The mean GBM thickness in our Saudi sample population was comparable to the very few reported measurements in the literature. There was no significant association between GBM thickness and gender; however, GBM thickness is directly proportional to age, up to 60 years old.
    MeSH term(s) Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Glomerular Basement Membrane/ultrastructure ; Humans ; Male ; Microscopy, Electron, Transmission ; Middle Aged ; Saudi Arabia ; Young Adult
    Language English
    Publishing date 2016-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 603269-2
    ISSN 1521-0758 ; 0191-3123
    ISSN (online) 1521-0758
    ISSN 0191-3123
    DOI 10.1080/01913123.2016.1199624
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  2. Article ; Online: Epithelial cell foot process effacement in podocytes in focal and segmental glomerulosclerosis: a quantitative analysis.

    Kfoury, Hala

    Ultrastructural pathology

    2014  Volume 38, Issue 5, Page(s) 303–308

    Abstract: Background/aim: Effacement of the epithelial cell foot processes of glomerular podocytes are thought to be diffuse in the primary form of focal and segmental glomerulosclerosis (FSGS). In contrast, effacements that occur in the secondary form of FSGS ... ...

    Abstract Background/aim: Effacement of the epithelial cell foot processes of glomerular podocytes are thought to be diffuse in the primary form of focal and segmental glomerulosclerosis (FSGS). In contrast, effacements that occur in the secondary form of FSGS are thought to be focal. To evaluate this theory, the extent of epithelial cell foot process effacement was analyzed and compared in podocytes from cases of primary and secondary FSGS.
    Methods: Consecutive cases of adult primary and secondary FSGS that were diagnosed between January 1997 and December 2010 were retrospectively retrieved and analyzed. The electron microscopy materials of all specimens were reviewed. Glomerular podocytes from each sample were counted, recorded and analyzed for the percentage of diffuse versus focal epithelial foot process effacement.
    Results: Seventeen primary and 16 secondary FSGS specimens were reviewed. In the 17 primary FSGS cases, 35.1% of the podocytes were focally fused, and 64.9% were diffusely fused. In the 16 secondary FSGS cases, 72.1% of the podocytes were focally fused. There were significantly more focally fused podocytes in secondary FSGS than in primary FSGS. In contrast, significantly more diffusely fused podocytes were observed in primary FSGS than in secondary FSGS.
    Conclusion: This is the first study to perform a quantitative analysis of the extent of epithelial cell foot process effacement in podocytes in primary and secondary FSGS. This study also confirms that the electron microscopic characteristics of the podocyte effacement process can be used to facilitate the diagnosis of primary versus secondary FSGS.
    MeSH term(s) Adolescent ; Adult ; Child ; Epithelial Cells/ultrastructure ; Female ; Glomerulosclerosis, Focal Segmental/pathology ; Humans ; Kidney Glomerulus/pathology ; Male ; Microscopy, Electron/methods ; Middle Aged ; Podocytes/ultrastructure ; Young Adult
    Language English
    Publishing date 2014-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 603269-2
    ISSN 1521-0758 ; 0191-3123
    ISSN (online) 1521-0758
    ISSN 0191-3123
    DOI 10.3109/01913123.2014.927405
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article: Tubulo-reticular inclusions in lupus nephritis: are they relevant?

    Kfoury, Hala

    Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia

    2014  Volume 25, Issue 3, Page(s) 539–543

    Abstract: Tubulo-reticular inclusions (TRIs) are organized subcellular structures that may be found in endothelial cells of patients with systemic lupus erythematosus (SLE). This study was conducted to determine the presence or absence of TRIs and their ... ...

    Abstract Tubulo-reticular inclusions (TRIs) are organized subcellular structures that may be found in endothelial cells of patients with systemic lupus erythematosus (SLE). This study was conducted to determine the presence or absence of TRIs and their correlation with the activity index (AI) and lupus nephritis (LN) class. A retrospective analysis of 57 cases of LN over a three-year period (2008-2011) was performed from medical records of the King Khalid University Hospital (KKUH), Riyadh. After reviewing and sorting them by class as per the International Society of Nephrology (ISN/RPS) 2004 classification of LN, the cases were divided according to the presence or absence of TRIs. The relationships between the presence or absence of TRIs and the AI were determined. Of the 57 kidney biopsies reviewed, 49 were proliferative cases (Class III and IV), of which 12 (24.5%) had TRIs. The mean AI was 6.01 ± 3.8 and the mean chronic index was 3.0 ± 1.5. Four (11.4%) class IV cases had a high AI, while no Class III cases with TRIs showed increased activity. The presence of TRIs was significantly associated with the AI (r = 9.40, P = 0.002), but not with LN class (r = 0.099, P = 0.753). Examining for TRIs in LN is still favorable and helpful in cases where the diagnosis of SLE is pending or not yet established, although the presence of TRIs is not a specific finding.
    MeSH term(s) Biopsy ; Cell Proliferation ; Hospitals, University ; Humans ; Inclusion Bodies/ultrastructure ; Kidney Tubules/ultrastructure ; Lupus Nephritis/pathology ; Lupus Nephritis/therapy ; Predictive Value of Tests ; Prognosis ; Retrospective Studies ; Saudi Arabia ; Severity of Illness Index
    Language English
    Publishing date 2014-05-04
    Publishing country Saudi Arabia
    Document type Journal Article
    ZDB-ID 1379955-1
    ISSN 1319-2442
    ISSN 1319-2442
    DOI 10.4103/1319-2442.132169
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  4. Article ; Online: A rare case of IgA lambda multiple myeloma in a 32-year-old woman with t(14;16) translocation associated with kidney injury and non-albumin proteinuria.

    Razzouk, Ranim / Khattab, Nour / Hoteit, Maysaa / Kfoury, Hala / Saleh, Mustafa / Tanios, Bassem / Cheikh, Jean El / Mallat, Samir

    BMC nephrology

    2024  Volume 25, Issue 1, Page(s) 165

    Abstract: Background: Multiple myeloma (MM) is a malignant disorder characterized by monoclonal differentiated plasma cells. While it is more commonly diagnosed in elderly individuals, it can also affect younger populations, though with a lower incidence.: Case ...

    Abstract Background: Multiple myeloma (MM) is a malignant disorder characterized by monoclonal differentiated plasma cells. While it is more commonly diagnosed in elderly individuals, it can also affect younger populations, though with a lower incidence.
    Case presentation: Here, we present the case of a 32-year-old woman diagnosed with IgA lambda MM. She presented with fatigue, nausea, acute kidney injury (AKI) with a rapid increase in creatinine, and anemia. A kidney biopsy was done to rule out a rapidly progressive glomerular disease and a diagnosis was thus reached. A genetic workup revealed t(14;16) translocation and an extra copy of TP53. The patient received aggressive intravenous steroids and intravenous fluid resuscitation, resulting in an improvement in renal function. Treatment with daratumumab in combination with bortezomib, thalidomide, and dexamethasone was initiated and well tolerated. Despite the generally poor prognosis of IgA MM, our case emphasizes the importance of considering MM in young patients with unexplained kidney injury.
    Conclusion: Early recognition and prompt intervention are essential in managing MM patients, especially in those with high-risk cytogenetic abnormalities. This case serves as a reminder for clinicians to maintain a high index of suspicion for MM, even in younger populations, when presented with unexplained kidney injury.
    MeSH term(s) Humans ; Female ; Adult ; Multiple Myeloma/complications ; Multiple Myeloma/genetics ; Multiple Myeloma/diagnosis ; Multiple Myeloma/drug therapy ; Proteinuria/etiology ; Translocation, Genetic ; Acute Kidney Injury/etiology ; Acute Kidney Injury/genetics ; Immunoglobulin A ; Immunoglobulin lambda-Chains/genetics ; Chromosomes, Human, Pair 14/genetics
    Language English
    Publishing date 2024-05-16
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 2041348-8
    ISSN 1471-2369 ; 1471-2369
    ISSN (online) 1471-2369
    ISSN 1471-2369
    DOI 10.1186/s12882-024-03600-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: The pathological spectrum associated with the ultrastructural finding of thin glomerular basement membrane: A tertiary medical city experience and review of the literature.

    Kfoury, Hala / Arafah, Maria

    Ultrastructural pathology

    2017  Volume 41, Issue 1, Page(s) 51–54

    Abstract: Background: Thin glomerular basement membrane (GBM) has been noted in several glomerular diseases including IgA nephropathy, focal segmental glomerulosclerosis (FSGS), Fabry's disease, and Alport's syndrome. We conducted this study to investigate the ... ...

    Abstract Background: Thin glomerular basement membrane (GBM) has been noted in several glomerular diseases including IgA nephropathy, focal segmental glomerulosclerosis (FSGS), Fabry's disease, and Alport's syndrome. We conducted this study to investigate the pathological ultrastructural spectrum of thin GBMs, to identify associated diseases, and to measure the GBM thickness in thin GBMs in our adult population.
    Materials and methods: All renal biopsies with thin GBM, diagnosed between 2010 and 2016, were retrieved and reviewed.
    Results: Of 24 cases, 50.0% were diagnosed with FSGS, 12.5% with IgA nephropathy, 8.3% with tubulointerstitial nephritis, 4.2% with acute thrombotic microangiopathy, 4.2% with focal global sclerosis, 4.2% with lupus nephritis, and 16.7% with only thin GBM disease. Mean GBM thickness was 213.4 ± 24.7 nm. Mean interstitial fibrosis/tubular atrophy percentage (IF/TA) was 27.9 ± 22.2%. There was no significant correlation between GBM thickness and patients' age or IF/TA percentage.
    Conclusion: The association of thin GBM with FSGS and IgA nephropathy is high. Morphometric analysis of the GBM thickness should be made routine, noting that ethnic variations in the GBM thickness are reported. Cases of thin GBM should be reported to facilitate proper diagnosis and institute the most appropriate treatment.
    MeSH term(s) Adolescent ; Adult ; Biopsy ; Female ; Glomerular Basement Membrane/ultrastructure ; Glomerulonephritis, IGA/diagnosis ; Glomerulonephritis, IGA/pathology ; Glomerulosclerosis, Focal Segmental/diagnosis ; Glomerulosclerosis, Focal Segmental/pathology ; Humans ; Kidney Diseases/diagnosis ; Kidney Diseases/ethnology ; Kidney Diseases/pathology ; Male ; Microscopy, Electron, Transmission ; Middle Aged ; Predictive Value of Tests ; Prognosis ; Retrospective Studies ; Saudi Arabia ; Tertiary Care Centers ; Young Adult
    Language English
    Publishing date 2017-01
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 603269-2
    ISSN 1521-0758 ; 0191-3123
    ISSN (online) 1521-0758
    ISSN 0191-3123
    DOI 10.1080/01913123.2016.1258021
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article: Status of mismatch repair genes hMSH2 and hMSH6 in colorectal cancer in Saudi patients: an immunohistochemical analysis.

    Alkhalidi, H / Kfoury, H

    Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit

    2012  Volume 18, Issue 11, Page(s) 1114–1117

    Abstract: This study aimed to identify the status of 2 major microsatellite instability markers (repair genes hMSH2 and hMSH6) in colorectal cancer cases operated at King Khalid University Hospital, Riyadh, Saudi Arabia between 2007 and 2009. Immunohistochemical ... ...

    Abstract This study aimed to identify the status of 2 major microsatellite instability markers (repair genes hMSH2 and hMSH6) in colorectal cancer cases operated at King Khalid University Hospital, Riyadh, Saudi Arabia between 2007 and 2009. Immunohistochemical study of microsatellite instability was done with antibodies to hMSH2 and hMSH6. A total of 32 blocks were analysed from patients aged 16-83 years (median 56 years); 14 blocks (43.8%) were from resections and 18 (56.2%) were from biopsies. An adenomatous component was present in 4 (12.5%) blocks. The colonic carcinoma, the adenomas and the normal tissue showed strong nuclear reactivity to hMSH2 and hMSH6 in 96.9% of the cases. The rate of loss of expression was 3.1%. The rate of mutation in our sampled population was low and matched the rate reported in the literature from industrialized countries. Further studies are needed to confirm the use of these markers in the diagnosis of colorectal cancer.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers ; Colorectal Neoplasms/epidemiology ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/surgery ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Female ; Humans ; Immunochemistry ; Male ; Microsatellite Instability ; Middle Aged ; MutS Homolog 2 Protein/genetics ; MutS Homolog 2 Protein/metabolism ; Saudi Arabia/epidemiology ; Young Adult
    Chemical Substances Biomarkers ; DNA-Binding Proteins ; G-T mismatch-binding protein ; MSH2 protein, human (EC 3.6.1.3) ; MutS Homolog 2 Protein (EC 3.6.1.3)
    Language English
    Publishing date 2012-12-12
    Publishing country Egypt
    Document type Journal Article
    ZDB-ID 1291071-5
    ISSN 1687-1634 ; 1020-3397
    ISSN (online) 1687-1634
    ISSN 1020-3397
    DOI 10.26719/2012.18.11.1114
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Book ; Online: Status of mismatch repair genes hMSH2 and hMSH6 in colorectal cancer in Saudi patients

    Alkhalidi, H. / Kfoury, H.

    an immunohistochemical analysis

    2012  

    Abstract: 1114-1117 ... This study aimed to identify the status of 2 major microsatellite instability markers [repair genes hMSH2and hMSH6] in colorectal cancer cases operated at King Khalid University Hospital, Riyadh, Saudi Arabia between 2007 and 2009. ... ...

    Abstract 1114-1117

    This study aimed to identify the status of 2 major microsatellite instability markers [repair genes hMSH2and hMSH6] in colorectal cancer cases operated at King Khalid University Hospital, Riyadh, Saudi Arabia between 2007 and 2009. Immunohistochemical study of microsatellite instability was done with antibodies to hMSH2and hMSH6. A total of 32 blocks were analysed from patients aged 16-83 years [median 56 years]; 14 blocks [43.8%] were from resections and 18 [56.2%] were from biopsies. An adenomatous component was present in 4 [12.5%] blocks. The colonic carcinoma, the adenomas and the normal tissue showed strong nuclear reactivity to hMSH2and hMSH6in 96.9% of the cases. The rate of loss of expression was 3.1%. The rate of mutation in our sampled population was low and matched the rate reported in the literature from industrialized countries. Further studies are needed to confirm the use of these markers in the diagnosis of colorectal cancer
    Keywords MutS Homolog 2 Protein ; Immunohistochemistry ; Microsatellite Instability ; DNA Mismatch Repair ; DNA-Binding Proteins ; Colorectal Neoplasms
    Language English
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  8. Article ; Online: VEGF-A: A Novel Mechanistic Link Between CYP2C-Derived EETs and Nox4 in Diabetic Kidney Disease.

    Njeim, Rachel / Braych, Kawthar / Ghadieh, Hilda E / Azar, Nadim S / Azar, William S / Dia, Batoul / Leone, Angelo / Cappello, Francesco / Kfoury, Hala / Harb, Frederic / Jurjus, Abdo R / Eid, Assaad A / Ziyadeh, Fuad N

    Diabetes

    2023  Volume 72, Issue 7, Page(s) 947–957

    Abstract: Diabetes is associated with decreased epoxyeicosatrienoic acid (EET) bioavailability and increased levels of glomerular vascular endothelial growth factor A (VEGF-A) expression. We examined whether a soluble epoxide hydrolase inhibitor protects against ... ...

    Abstract Diabetes is associated with decreased epoxyeicosatrienoic acid (EET) bioavailability and increased levels of glomerular vascular endothelial growth factor A (VEGF-A) expression. We examined whether a soluble epoxide hydrolase inhibitor protects against pathologic changes in diabetic kidney disease and whether the inhibition of the VEGF-A signaling pathway attenuates diabetes-induced glomerular injury. We also aimed to delineate the cross talk between cytochrome P450 2C (CYP2C)-derived EETs and VEGF-A. Streptozotocin-induced type 1 diabetic (T1D) rats were treated with 25 mg/L of 12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA) in drinking water for 6 weeks. In parallel experiments, T1D rats were treated with either SU5416 or humanized monoclonal anti-VEGF-A neutralizing antibody for 8 weeks. Following treatment, the rats were euthanized, and kidney cortices were isolated for further analysis. Treatment with AUDA attenuated the diabetes-induced decline in kidney function. Furthermore, treatment with AUDA decreased diabetes-associated oxidative stress and NADPH oxidase activity. Interestingly, the downregulation of CYP2C11-derived EET formation is found to be correlated with the activation of the VEGF-A signaling pathway. In fact, inhibiting VEGF-A using anti-VEGF or SU5416 markedly attenuated diabetes-induced glomerular injury through the inhibition of Nox4-induced reactive oxygen species production. These findings were replicated in vitro in rat and human podocytes cultured in a diabetic milieu. Taken together, our results indicate that hyperglycemia-induced glomerular injury is mediated by the downregulation of CYP2C11-derived EET formation, followed by the activation of VEGF-A signaling and upregulation of Nox4. To our knowledge, this is the first study to highlight VEGF-A as a mechanistic link between CYP2C11-derived EET production and Nox4.
    Article highlights: Diabetes is associated with an alteration in cytochrome P450 2C11 (CYP2C11)-derived epoxyeicosatrienoic acid (EET) bioavailability. Decreased CYP2C11-derived EET bioavailability mediates hyperglycemia-induced glomerular injury. Decreased CYP2C11-derived EET bioavailability is associated with increased reactive oxygen species production, NADPH oxidase activity, and Nox4 expression in type 1 diabetes. Decreased CYP2C11-derived EET formation mediates hyperglycemia-induced glomerular injury through the activation of the vascular endothelial growth factor A (VEGF-A) signaling pathway. Inhibiting VEGF signaling using anti-VEGF or SU5416 attenuates type 1 diabetes-induced glomerular injury by decreasing NADPH oxidase activity and NOX4 expression.
    MeSH term(s) Rats ; Animals ; Humans ; Diabetic Nephropathies ; Vascular Endothelial Growth Factor A ; Diabetes Mellitus, Type 1 ; Reactive Oxygen Species/metabolism ; Cytochrome P-450 Enzyme System ; Hyperglycemia ; NADPH Oxidase 4/genetics
    Chemical Substances cytochrome P-450 CYP2C subfamily ; Vascular Endothelial Growth Factor A ; Reactive Oxygen Species ; Cytochrome P-450 Enzyme System (9035-51-2) ; Nox4 protein, rat (EC 1.6.3.-) ; NADPH Oxidase 4 (EC 1.6.3.-)
    Language English
    Publishing date 2023-01-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80085-5
    ISSN 1939-327X ; 0012-1797
    ISSN (online) 1939-327X
    ISSN 0012-1797
    DOI 10.2337/db22-0636
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article: What's your diagnosis? Mycetomas.

    Kfoury, H / Al Fadley, A

    Annals of Saudi medicine

    2007  Volume 17, Issue 4, Page(s) 441–443

    Language English
    Publishing date 2007-01-28
    Publishing country Saudi Arabia
    Document type Journal Article
    ZDB-ID 639014-6
    ISSN 0975-4466 ; 0256-4947
    ISSN (online) 0975-4466
    ISSN 0256-4947
    DOI 10.5144/0256-4947.1997.441
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article: Reply.

    Kfoury, H / Al Bhlal, L

    Annals of Saudi medicine

    2007  Volume 18, Issue 1, Page(s) 87

    Language English
    Publishing date 2007-01-28
    Publishing country Saudi Arabia
    Document type Letter
    ZDB-ID 639014-6
    ISSN 0975-4466 ; 0256-4947
    ISSN (online) 0975-4466
    ISSN 0256-4947
    DOI 10.5144/0256-4947.1998.87
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top