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  1. Article: Diabetic Retinopathy Screening Using Non-Mydriatic Fundus Camera in Primary Health Care Settings - A Multicenter Study from Saudi Arabia.

    Alabdulwahhab, Khalid Mohammad

    International journal of general medicine

    2023  Volume 16, Page(s) 2255–2262

    Abstract: Background: Screening of diabetic retinopathy (DR) using the current digital imaging facilities in a primary health care setting is still in its early stages in Saudi Arabia. This study aims to reduce the risk of vision impairment and blindness among ... ...

    Abstract Background: Screening of diabetic retinopathy (DR) using the current digital imaging facilities in a primary health care setting is still in its early stages in Saudi Arabia. This study aims to reduce the risk of vision impairment and blindness among known diabetic people through early identification by general practitioners (GP) in a primary health care setting in Saudi Arabia. The objective of this study was to evaluate the accuracy of diabetic retinopathy (DR) detection by general practitioners (GPs) by comparing the agreement of DR assessment between GPs and ophthalmologists' assessment as a gold standard.
    Methods: A hospital-based, six-month cross-sectional study was conducted, and the participants were type 2 diabetic adults from the diabetic registries of seven rural PHCs, in Saudi Arabia. After medical examination, the participants were then evaluated by fundus photography using a non-mydriatic fundus camera without medication for mydriasis. Presence or absence of DR was graded by the trained GPs in the PHCs and then compared with the grading of an ophthalmologist which was taken as a reference or a gold standard.
    Results: A total of 899 diabetic patients were included, and the mean age of the patients was 64.89 ± 11.01 years. The evaluation by the GPs had a sensitivity of 80.69 [95% CI 74.8-85.4]; specificity of 92.23 [88.7-96.3]; positive predictive value, 74.1 [70.4-77.0]; negative predictive value, 73.34 [70.6-77.9]; and an accuracy of 84.57 [81.8-89.88]. For the consensus of agreement the adjusted kappa coefficient was from 0.74 to 0.92 for the DR.
    Conclusion: This study demonstrates that trained GPs in rural health centers are able to provide reliable detection results of DR from fundus photographs. The study highlights the need for early DR screening programs in the rural areas of Saudi Arabia to facilitate early identification of the condition and to lessen impact of blindness due to diabetes.
    Language English
    Publishing date 2023-06-05
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2452220-X
    ISSN 1178-7074
    ISSN 1178-7074
    DOI 10.2147/IJGM.S410197
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Host independent deletion hotspots in the SARS-CoV-2 genome

    Khalid, Mohammad

    bioRxiv

    Keywords covid19
    Language English
    Publishing date 2022-10-17
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2022.10.16.512395
    Database COVID19

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  3. Article ; Online: Senile Cataract in Patients with Diabetes with and Without Diabetic Retinopathy: A Community-Based Comparative Study.

    Alabdulwahhab, Khalid Mohammad

    Journal of epidemiology and global health

    2021  Volume 12, Issue 1, Page(s) 56–63

    Abstract: Aim: We compare the incidence rates of cataract in persons with diabetes with and without diabetic retinopathy in Saudi Arabia, for the first time. In addition, we explored the role of new factor, diabetes age of onset and several other known factors.!## ...

    Abstract Aim: We compare the incidence rates of cataract in persons with diabetes with and without diabetic retinopathy in Saudi Arabia, for the first time. In addition, we explored the role of new factor, diabetes age of onset and several other known factors.
    Methods: In a community-based cross-sectional study, 334 persons with diabetes type 2 were randomly selected from a diabetic register. Detailed history and comprehensive ophthalmic examination was done at an eye clinic. Body Mass Index, blood pressure and glycosylated hemoglobin were also recorded.
    Results: In 668 eyes, cataract and diabetic retinopathy were present in 35.5% and 32.2%, respectively. Diabetic retinopathy, age, duration of diabetes and systolic BP were found to be independent risk factors for cataract. Whereas, gender, BMI, HbA1c use of insulin and diastolic BP have no significant association with cataract. Persons with cataract had significantly higher age of onset of diabetes. Most of the cataracts were cortical followed by PSC, while minority were nuclear.
    Conclusion: DR is an independent risk factor of developing cataract in persons with diabetes. Others are age, duration of DM and hypertension. Age-of-onset of DM is a new factor we report it to be significantly associated with cataract.
    MeSH term(s) Cataract/complications ; Cataract/epidemiology ; Cross-Sectional Studies ; Diabetes Mellitus, Type 2/epidemiology ; Diabetic Retinopathy/epidemiology ; Diabetic Retinopathy/etiology ; Glycated Hemoglobin A/analysis ; Humans ; Prevalence ; Risk Factors
    Chemical Substances Glycated Hemoglobin A
    Language English
    Publishing date 2021-12-07
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2645324-1
    ISSN 2210-6014 ; 2210-6014
    ISSN (online) 2210-6014
    ISSN 2210-6014
    DOI 10.1007/s44197-021-00020-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Evaluations of FDA-approved drugs targeting 3CLP of SARS-CoV-2 employing a repurposing strategy.

    Ahmad, Syed Sayeed / Khalid, Mohammad

    Combinatorial chemistry & high throughput screening

    2022  

    Abstract: Background: The SARS-CoV-2 coronavirus (COVID-19) has raised innumerable global concerns, and few effective treatment strategy has yet been permitted by the FDA to lighten the disease burden. SARS-CoV-2 3C-like proteinase (3CLP) is a crucial protease ... ...

    Abstract Background: The SARS-CoV-2 coronavirus (COVID-19) has raised innumerable global concerns, and few effective treatment strategy has yet been permitted by the FDA to lighten the disease burden. SARS-CoV-2 3C-like proteinase (3CLP) is a crucial protease and plays a key role in the viral life cycle, as it controls replication, and thus, it is viewed as a target for drug design.
    Method: In this study, we performed structure-based virtual screening of FDA drugs approved during the period 2015-2019 (total 220 drugs) for interaction with the active site of 3CLP (PDB ID 6LU7) using AutoDock 4.2. We report the top ten drugs that outperform the reported drugs against 3CLP (Elbasvir and Nelfinavir), particularly Cefiderocol having the highest affinity among the compounds tested, with a binding energy of -9.97 kcal/mol. H-bond (LYS102:HZ2-ligand:O49), hydrophobic (ligand-VAL104), and electrostatic (LYS102:NZ-ligand:O50) interactions were observed in cefiderocol-3CLP complex. The docked complex was subjected to a 50 ns molecular dynamics study to check its stability, and stable RMSD and RMSF graphs were observed.
    Result: Accordingly, we suggest cefiderocol might be effective against SARS-CoV-2 and urge that experimental validation to be performed to determine the antiviral efficacy of cefiderocol against SARS-CoV-2.
    Discussion: Along with these, cefiderocol is effective for the treatment of respiratory tract pathogens and wide range of gram-negative bacteria for whom there are limited therapeutic alternatives.
    Conclusion: The aim of this article was to explore the FDA approved drugs as repurposing study against 3CLP for COVID-19 management.
    Language English
    Publishing date 2022-08-16
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2064785-2
    ISSN 1875-5402 ; 1386-2073
    ISSN (online) 1875-5402
    ISSN 1386-2073
    DOI 10.2174/1386207325666220816125639
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Kenaf-based activated carbon: A sustainable solution for high-performance aqueous symmetric supercapacitors.

    Subramaniam, Thilageshwaran / Ansari, M N M / Krishnan, Syam G / Khalid, Mohammad

    Chemosphere

    2024  Volume 354, Page(s) 141593

    Abstract: This study presents an innovative method for synthesizing activated carbon with an exceptionally high surface area (3359 ... ...

    Abstract This study presents an innovative method for synthesizing activated carbon with an exceptionally high surface area (3359 m
    MeSH term(s) Charcoal ; Hibiscus ; Electric Capacitance ; Electrodes
    Chemical Substances Charcoal (16291-96-6)
    Language English
    Publishing date 2024-03-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2024.141593
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Impact of UV radiation on Mxene-mediated tubulin dissociation and mitochondrial apoptosis in breast cancer cells.

    Tan, Ee Wern / Simon, Samson Eugin / Numan, Arshid / Khalid, Mohammad / Tan, Kuan Onn

    Colloids and surfaces. B, Biointerfaces

    2024  Volume 235, Page(s) 113793

    Abstract: Breast cancer is a global health concern that requires personalized therapies to prevent relapses, as conventional treatments may develop resistance over time. Photothermal therapy using spectral radiation or intense light emission is a broad-spectrum ... ...

    Abstract Breast cancer is a global health concern that requires personalized therapies to prevent relapses, as conventional treatments may develop resistance over time. Photothermal therapy using spectral radiation or intense light emission is a broad-spectrum treatment that induces hyperthermia-mediated cancer cell death. MXene, a two-dimensional material, has been reported to have potential biological applications in photothermal therapy for cancer treatment. In this study, we investigated the apoptotic activity of MXene and UV-irradiated MXene in MCF-7 breast cancer cells by treating them with varying concentrations of MXene. The cytotoxicity of MXene and UV was evaluated by analyzing cellular morphology, nuclei condensation, caspase activation, and apoptotic cell death. We also assessed the effect of the combined treatment on the expression and cellular distribution of Tubulin, a key component of microtubules required for cell division. At low concentrations of MXene (up to 100 µg/ml), the level of cytotoxicity in MCF-7 cells was low. However, the combined treatment of MXene and UV resulted in a synergistic increase in cytotoxicity, causing rounded cellular morphology, condensed nuclei, caspase activation, and apoptotic cell death. Furthermore, the treatment reduced Tubulin protein expression and cellular distribution, indicating a potent inducer of cell death with potential application for cancer treatment. The study demonstrates that the combined treatment of MXene and UVB irradiation is a promising strategy for inducing apoptotic cell death in breast cancer cells, suggesting its potential as a therapeutic intervention for breast cancer.
    MeSH term(s) Humans ; Female ; Ultraviolet Rays ; Tubulin ; Breast Neoplasms/metabolism ; Apoptosis ; MCF-7 Cells ; Caspase 3/metabolism ; Cell Line, Tumor ; Nitrites ; Transition Elements
    Chemical Substances Tubulin ; MXene ; Caspase 3 (EC 3.4.22.-) ; Nitrites ; Transition Elements
    Language English
    Publishing date 2024-02-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1500523-9
    ISSN 1873-4367 ; 0927-7765
    ISSN (online) 1873-4367
    ISSN 0927-7765
    DOI 10.1016/j.colsurfb.2024.113793
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Investigating the adsorption potential of char derived from waste latex for methylene blue removal.

    Nagarajan, Thachnatharen / Vilosamy, Khirthiga / Raju, Gunasunderi / Shanmugan, Subramani / Walvekar, Rashmi / Rustagi, Sarvesh / Khalid, Mohammad

    Chemosphere

    2024  Volume 358, Page(s) 141936

    Abstract: This study presents the adsorption of methylene blue (MB) dye using latex char derived from pyrolysis of latex gloves. The adsorption process was investigated systematically using Response Surface Methodology (RSM) with a Central Composite Design (CCD). ... ...

    Abstract This study presents the adsorption of methylene blue (MB) dye using latex char derived from pyrolysis of latex gloves. The adsorption process was investigated systematically using Response Surface Methodology (RSM) with a Central Composite Design (CCD). The effects of four key variables, namely pH, time, temperature, and adsorbent dosage, were studied using a factorial design enriched with center points and axial points. Experimental data were analyzed using a second-order polynomial regression model to construct a response surface model, which elucidated the relationship between the variables and MB removal efficiency. The study found that the char obtained at 800 °C exhibited the highest adsorption capacity due to its increased carbonization, expanded surface area, and diverse pore structure. Analysis of Variance (ANOVA) confirmed the significance of the quadratic model, with remarkable agreement between predicted and experimental outcomes. Diagnostic plots validated the model's reliability, while 3D contour graphs illustrated the combined effects of variables on MB removal efficiency. Optimization using DoE software identified optimal conditions resulting in a 99% removal efficiency, which closely matched experimental results. Additionally, adsorption isotherms revealed that the Freundlich model best described the adsorption behavior, indicating heterogeneous surface adsorption with multilayer adsorption. This comprehensive study provides valuable insights into the adsorption process of MB dye using latex char, with implications for wastewater treatment and environmental remediation.
    Language English
    Publishing date 2024-04-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2024.141936
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Genome similarities between human-derived and mink-derived SARS-CoV-2 make mink a potential reservoir of the virus

    Khalid, Mohammad / Al-ebini, Yousef

    bioRxiv

    Abstract: The SARS-CoV-2 has RNA as the genome, which makes the virus more prone to mutations. Occasionally, mutations help a virus to cross the species barrier. The SARS-CoV-2 infection to humans and minks (Neovison vison) are examples of zoonotic spillover. Many ...

    Abstract The SARS-CoV-2 has RNA as the genome, which makes the virus more prone to mutations. Occasionally, mutations help a virus to cross the species barrier. The SARS-CoV-2 infection to humans and minks (Neovison vison) are examples of zoonotic spillover. Many studies have been published on the analysis of human-derived SARS-CoV-2, here we performed mutation analysis on the minks-derived SARS-CoV-2 genome sequences. We analyzed all available full-length mink derived SARS-CoV-2 genome sequences on GISAID (214 from Netherlands and 133 from Denmark). We found that the mutation pattern in the Netherlands and Denmark derived samples were different. Out of a total of 201 mutations, we found in this study, only 13 mutations were common in the Netherlands and Denmark derived samples. We found 4 mutations prevailed in the Netherlands and Denmark mink derived samples and these 4 mutations are also reported to prevail in human-derived SARS-CoV-2.
    Keywords covid19
    Language English
    Publishing date 2022-05-30
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2022.05.29.493871
    Database COVID19

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  9. Article: Optimization of chromatographic conditions via Box‒Behnken design in RP-HPLC-PDA method development for the estimation of folic acid and methotrexate in bulk and tablets.

    Alshehri, Saad Ali / Wahab, Shadma / Khalid, Mohammad / Almoyad, Mohammad Ali Abdullah

    Heliyon

    2023  Volume 9, Issue 10, Page(s) e20282

    Abstract: Simultaneous estimation of folic acid and methotrexate in bulk and tablet dosage form by RP-HPLC-PDA was conducted via Box‒Behnken design application. Three-factor numerical values were finalized from the graphical and numerical optimization with built- ... ...

    Abstract Simultaneous estimation of folic acid and methotrexate in bulk and tablet dosage form by RP-HPLC-PDA was conducted via Box‒Behnken design application. Three-factor numerical values were finalized from the graphical and numerical optimization with built-in ANOVA in BBD. Sharp and symmetric peaks were observed at 4.138 and 6.929 min for folic acid and methotrexate, respectively. The mobile phase composition was methanol and 0.1% formic acid in water with a ratio of 31:69 and a flow rate of 1.1 ml/min. Both drugs were detected at a wavelength of 291 nm. The developed method was validated according to ICH guidelines. The results of the validation parameters were within acceptable limits. Stress stability studies have been performed under acidic, alkali, oxidation, neutral and photolytic conditions. Three different brand-marketed tablets were assessed with the developed method (MGXT, FOLTNAX and TRUXOFOL). In the tablet formulations, chromatogram percentages of folic acid and methotrexate were calculated at 99.13% and 99.50 in MGXT, 99.17% and 99.47 in FOLTNAX, and 99.91 and 100.05 in TRUXOFOL.
    Language English
    Publishing date 2023-09-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e20282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: A review of synthetic strategy, SAR, docking, simulation studies, and mechanism of action of isoxazole derivatives as anticancer agents.

    Arya, Girish Chandra / Khalid, Mohammad / Mehla, Shefali / Jakhmola, Vikash

    Journal of biomolecular structure & dynamics

    2023  Volume 42, Issue 9, Page(s) 4909–4935

    Abstract: Breast cancer (BC) is a global health concern and the leading cause of cancerous death among women across the world, BC has been characterized by fresh lump in the breast or underarm (armpit), thickened or swollen. Worldwide estimated 9.6 million deaths ... ...

    Abstract Breast cancer (BC) is a global health concern and the leading cause of cancerous death among women across the world, BC has been characterized by fresh lump in the breast or underarm (armpit), thickened or swollen. Worldwide estimated 9.6 million deaths in 2018-2019. Numerous drugs have been approved by FDA for BC treatment but showed numerous adverse effects like bioavailability issues, selectivity issues, and toxicity issues. Therefore, there is an immediate need to develop new molecules that are non-toxic and more efficient for treating cancer. Isoxazole derivatives have gained popularity over the few years due to their effective antitumor potential. These derivatives work against cancer by inhibiting the thymidylate enzyme, inducing apoptosis, inhibiting tubulin polymerization, protein kinase inhibition, and aromatase inhibition. In this study, we have concentrated on the isoxazole derivative with structure-activity relationship study, various synthesis techniques, mechanism of action, docking, and simulation studies pertaining to BC receptors. Hence the development of isoxazole derivatives with improved therapeutic efficacy will inspire further progress in improving human health.Communicated by Ramaswamy H. Sarma.
    MeSH term(s) Humans ; Molecular Docking Simulation ; Structure-Activity Relationship ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/chemistry ; Isoxazoles/chemistry ; Isoxazoles/pharmacology ; Molecular Dynamics Simulation ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Breast Neoplasms/metabolism ; Female
    Language English
    Publishing date 2023-06-14
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2023.2220819
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