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  1. Article: A Two-Year Study on Yield and Yield Components of Maize-White Bean Intercropping Systems under Different Sowing Techniques

    Zhanbota, Aidyn / Noor, Rana Shahzad / Khan, Azeem Iqbal / Wang, Gangyi / Waqas, Muhammad Mohsin / Shah, Adnan Noor / Ullah, Sami

    Agronomy. 2022 Jan. 18, v. 12, no. 2

    2022  

    Abstract: Food security is directly coupled with enhanced production under optimized cropping intensity. Intercropping is a diversified and sustainable agricultural technique with optimized cropping intensity. Intercropping is used to obtain a higher yield and ... ...

    Abstract Food security is directly coupled with enhanced production under optimized cropping intensity. Intercropping is a diversified and sustainable agricultural technique with optimized cropping intensity. Intercropping is used to obtain a higher yield and more balanced products per unit area. This study was performed at Aidyn Research Institute, Nur Sultan, Kazakhstan, in 2018 and 2019 to identify the effects of different sowing patterns on maize-white bean (Zea mays–Phaseolus vulgaris) sowing systems. The field experiment was arranged in a randomized complete block design with three replications. Göynük-98 was used for white beans, and SY Miami was used for maize, with 20 cm and 40 cm row spaces for maize, and 10 cm and 20 cm row spaces for white bean and sole maize, sole white bean, maize-white bean-maize-white bean, maize-white bean-white bean-maize and white bean-maize-maize-white bean sowing systems. The results showed that wide row spacing was better than narrow row spacing in terms of land equivalent ratio (LER) for both maize and white beans, but grain yield was higher in narrow row spacing. Yield items for both maize and white beans showed higher values in intercropping. Grain yield was higher in sole sowing. The maize-white bean-white bean-maize sowing system for maize and the white bean-maize-maize-white bean sowing system for white beans were determined as the best sowing systems according to the yield components.
    Keywords Zea ; agronomy ; corn ; field experimentation ; food security ; grain yield ; research institutions ; Kazakhstan
    Language English
    Dates of publication 2022-0118
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2607043-1
    ISSN 2073-4395
    ISSN 2073-4395
    DOI 10.3390/agronomy12020240
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Single nucleotide polymorphism based assessment of genetic diversity in local and exotic onion genotypes

    Tabussam, Najma / Rana, Rashid Mehmood / Wattoo, Fahad Masoud / Khan, Azeem Iqbal / Amir, Rai Muhammad / Javed, Talha / Ahmar, Sunny / Dessoky, Eldessoky S. / Abdelsalam, Nader R.

    Molecular biology reports. 2022 June, v. 49, no. 6

    2022  

    Abstract: BACKGROUND: Onion is an economically important vegetable cultivated worldwide on a large scale. Liberal exchange of germplasm and frequent selection caused narrow genetic diversity in most crops, including onion. Thus, it is essential to estimate and ... ...

    Abstract BACKGROUND: Onion is an economically important vegetable cultivated worldwide on a large scale. Liberal exchange of germplasm and frequent selection caused narrow genetic diversity in most crops, including onion. Thus, it is essential to estimate and understand genetic diversity before launching of any breeding program. The current study was conducted to explore genetic diversity among 39 short-day onion genotypes (indigenous and exotic). METHODS AND RESULTS: All the genotypes were evaluated for various phenotypic traits by using single nucleotide polymorphism (SNP) genotyping based on KASPar assays. Principal component analysis (PCA) was performed to determine the variability among genotypes. The four principal components with eigenvalue greater than 1 accounted for 67.5656% variability for quantitative traits, whereas first five principal components with eigenvalue greater than 0.7 accounted for 86.24% variation among the genotypes for qualitative traits. The principal component analysis identified diverse traits including bulb weight, bulb diameter, plant height, number of survived plants and vitamin C. These traits were further analyzed through ANOVA (Analysis of Variance) following augmented block design to describe genotypic variability for selected traits. Onion genotypes showed significant variation for bulb weight, bulb diameter and Vitamin C. Genotypic clustering based on PCA showed that 15 indigenous genotypes were clustered with exotic genotypes (14) while remaining indigenous genotypes (10) were distant. A total of 30 SNPs were used for assessment of genetic diversity out of these, 24 SNPs were detected with polymorphic loci (0.8%, heterozygosity), while only six markers were with monomorphic sites (0.2% heterozygosity). Subsequently, population structure analysis revealed three different populations indicating significant variability. CONCLUSION: Conclusively, a significant similarity between exotic and a group of indigenous genotypes indicates direct adoption of exotic genotypes or their sister lines. A further broadening of the genetic base is required and could be done by crossing distant genotypes.
    Keywords analysis of variance ; ascorbic acid ; bulbs ; genetic background ; genetic variation ; genotyping ; germplasm ; heterozygosity ; molecular biology ; onions ; phenotype ; plant height ; population structure ; principal component analysis ; single nucleotide polymorphism
    Language English
    Dates of publication 2022-06
    Size p. 5511-5520.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-022-07431-z
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Drought and heat stress on cotton genotypes suggested agro-physiological and biochemical features for climate resilience.

    Zafar, Muhammad Mubashar / Chattha, Waqas Shafqat / Khan, Azeem Iqbal / Zafar, Saba / Subhan, Mishal / Saleem, Huma / Ali, Arfan / Ijaz, Aqsa / Anwar, Zunaira / Qiao, Fei / Shakeel, Amir / Seleiman, Mahmoud F / Wasonga, Daniel O / Parvaiz, Aqsa / Razzaq, Abdul / Xuefei, Jiang

    Frontiers in plant science

    2023  Volume 14, Page(s) 1265700

    Abstract: This study aimed to investigate the impact of individual drought, heat, and combined drought and heat stress on twelve cotton genotypes, including eight tolerant and four susceptible genotypes. A field experiment was carried out by employing a randomized ...

    Abstract This study aimed to investigate the impact of individual drought, heat, and combined drought and heat stress on twelve cotton genotypes, including eight tolerant and four susceptible genotypes. A field experiment was carried out by employing a randomized complete block split-plot design, with treatments (control, drought, heat, drought + heat), and cotton genotypes assigned to the main plots and sub-plots respectively. The results showed that the combined stress had a more severe impact on the yield and fiber quality of cotton genotypes compared to individual stresses. Among the studied genotypes, FB-Shaheen, FH-207, MNH-886, and White Gold exhibited superior performance in regard to agronomic and fiber quality characters under combined stress environments. Physiological parameters, including transpiration rate, stomatal conductance, relative water contents, and photosynthetic rate, were significantly reduced under combined stress. However, specific genotypes, MNH-886, FH-207, White Gold, and FB-Shaheen, demonstrated better maintenance of these parameters, indicating their enhanced tolerance to the combined stress. Furthermore, the accumulation of reactive oxygen species was more pronounced under combined stress compared to individual stressors. Tolerant genotypes showed lower levels of H
    Language English
    Publishing date 2023-10-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1265700
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Identification and lead-in characterization of novel B3 metallo-β-lactamases.

    Mateen, Irsa / Awan, Faisal Saeed / Khan, Azeem Iqbal / Zia, Muhammad Anjum

    Pakistan journal of pharmaceutical sciences

    2017  Volume 30, Issue 1 Suppl, Page(s) 335–340

    Abstract: Metallo-β-lactamases (MBLs) are zinc ion dependent enzymes that are responsible for the emergence and spread of β-lactam resistance among bacterial pathogens. There are uncharacterized putative MBLs in the environment and their emergence is major ... ...

    Abstract Metallo-β-lactamases (MBLs) are zinc ion dependent enzymes that are responsible for the emergence and spread of β-lactam resistance among bacterial pathogens. There are uncharacterized putative MBLs in the environment and their emergence is major interference in the generation of universal MBL inhibitors so it is important to identify and characterize novel MBLs. In this study two novel MBLs from Luteimonas sp. J29 and Pseudoxanthomonas mexicana were identified using B3 MBLs as query in BLAST database search. 3D models of putative MBLs generated by SWISS-MODEL server taking AIM-1 as a structural template were verified using web based structure assessment and validation programs. Multiple sequence alignment revealed that residues important for substrate binding were conserved and loop region residues (156-162 and 223-230) important for catalysis are variable in these novel MBLs. Homology models showed typical MBL α/β/β/α sandwich fold containing six α helices, twelve β strands and metal interacting residues are conserved in similar way as with other B3 MBLs. We report promising putative B3 MBLs with some variations and substrate docking studies revealed that novel MBLs have attributes close to acquired B3 MBLs.
    MeSH term(s) Amino Acid Sequence ; Catalytic Domain/physiology ; Sequence Alignment ; Substrate Specificity/physiology ; Zinc/metabolism ; beta-Lactamases/metabolism ; beta-Lactams/metabolism
    Chemical Substances beta-Lactams ; beta-Lactamases (EC 3.5.2.6) ; Zinc (J41CSQ7QDS)
    Language English
    Publishing date 2017-01
    Publishing country Pakistan
    Document type Journal Article
    ZDB-ID 885131-1
    ISSN 1011-601X
    ISSN 1011-601X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Single nucleotide polymorphism based assessment of genetic diversity in local and exotic onion genotypes.

    Tabussam, Najma / Rana, Rashid Mehmood / Wattoo, Fahad Masoud / Khan, Azeem Iqbal / Amir, Rai Muhammad / Javed, Talha / Ahmar, Sunny / Dessoky, Eldessoky S / Abdelsalam, Nader R

    Molecular biology reports

    2022  Volume 49, Issue 6, Page(s) 5511–5520

    Abstract: Background: Onion is an economically important vegetable cultivated worldwide on a large scale. Liberal exchange of germplasm and frequent selection caused narrow genetic diversity in most crops, including onion. Thus, it is essential to estimate and ... ...

    Abstract Background: Onion is an economically important vegetable cultivated worldwide on a large scale. Liberal exchange of germplasm and frequent selection caused narrow genetic diversity in most crops, including onion. Thus, it is essential to estimate and understand genetic diversity before launching of any breeding program. The current study was conducted to explore genetic diversity among 39 short-day onion genotypes (indigenous and exotic).
    Methods and results: All the genotypes were evaluated for various phenotypic traits by using single nucleotide polymorphism (SNP) genotyping based on KASPar assays. Principal component analysis (PCA) was performed to determine the variability among genotypes. The four principal components with eigenvalue greater than 1 accounted for 67.5656% variability for quantitative traits, whereas first five principal components with eigenvalue greater than 0.7 accounted for 86.24% variation among the genotypes for qualitative traits. The principal component analysis identified diverse traits including bulb weight, bulb diameter, plant height, number of survived plants and vitamin C. These traits were further analyzed through ANOVA (Analysis of Variance) following augmented block design to describe genotypic variability for selected traits. Onion genotypes showed significant variation for bulb weight, bulb diameter and Vitamin C. Genotypic clustering based on PCA showed that 15 indigenous genotypes were clustered with exotic genotypes (14) while remaining indigenous genotypes (10) were distant. A total of 30 SNPs were used for assessment of genetic diversity out of these, 24 SNPs were detected with polymorphic loci (0.8%, heterozygosity), while only six markers were with monomorphic sites (0.2% heterozygosity). Subsequently, population structure analysis revealed three different populations indicating significant variability.
    Conclusion: Conclusively, a significant similarity between exotic and a group of indigenous genotypes indicates direct adoption of exotic genotypes or their sister lines. A further broadening of the genetic base is required and could be done by crossing distant genotypes.
    MeSH term(s) Ascorbic Acid ; Genetic Variation/genetics ; Genotype ; Onions/genetics ; Plant Breeding ; Polymorphism, Single Nucleotide/genetics
    Chemical Substances Ascorbic Acid (PQ6CK8PD0R)
    Language English
    Publishing date 2022-04-28
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-022-07431-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Exploiting Agronomic and Biochemical Traits to Develop Heat Resilient Cotton Cultivars under Climate Change Scenarios

    Zafar, Muhammad Mubashar / Manan, Abdul / Razzaq, Abdul / Zulfqar, Misbah / Saeed, Asif / Kashif, Muhammad / Khan, Azeem Iqbal / Sarfraz, Zareen / Mo, Huijuan / Iqbal, Muhammad Shahid / Shakeel, Amir / Ren, Maozhi

    Agronomy. 2021 Sept. 20, v. 11, no. 9

    2021  

    Abstract: The development of high-yielding heat-tolerant cotton cultivars harboring plastic phenotypes across warming climatic regions is prime objectives of today’s cotton breeding programs. We evaluated eight parents and 15 F₁ hybrids under normal and heat ... ...

    Abstract The development of high-yielding heat-tolerant cotton cultivars harboring plastic phenotypes across warming climatic regions is prime objectives of today’s cotton breeding programs. We evaluated eight parents and 15 F₁ hybrids under normal and heat stress conditions. Agronomic and biochemical characters were analyzed using standard least square, correlation, principal component analysis (PCA), and hierarchical clustering. The results explained a significant reduction in all traits except hydrogen peroxide contents, catalase, and peroxidase activities with a prominent increase under heat stress. A significant positive correlation was observed among all agronomic and biochemical traits. POD was found to have a maximum positive correlation with CAT (0.947) and minimum with boll weight (0.050). PCA showed first two components accounting for 78.64% of the total variation, with 55.83% and 22.80% of the total variation, respectively. Based on multivariate analyses methods 23 genotypes have been placed in 3 groups: tolerant (cluster-3), moderately tolerant (cluster-2), and susceptible (cluster-1). In a general perspective hybrids have better performance across normal and heat stress supports the idea of hybrid adaptability across stress environments. In specific FH-458 × FH-313 cross performed best across both conditions for yield and physiological traits. Hence, the generated information from the present study would support breeders in developing heat-resilient cultivars to endure the prevailing extreme environmental conditions.
    Keywords agronomy ; catalase ; climate change ; cotton ; cultivars ; heat ; heat stress ; heat tolerance ; hydrogen peroxide ; peroxidase ; principal component analysis
    Language English
    Dates of publication 2021-0920
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2607043-1
    ISSN 2073-4395
    ISSN 2073-4395
    DOI 10.3390/agronomy11091885
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm

    Sher Muḥammad / Sajjād, Muḥammad / Khan, Sultan Habibullah / Shāhid, Muḥammad / Zubair, Muhammad / Awan, Faisal Saeed / Khan, Azeem Iqbal / Mubarak, Muhammad Salman / Tahir, Ayesha / Umer, Muhammad / Keyani, Rumana / Afzal, Muhammad Inam / Manzoor, Irfan / Wattoo, Javed Iqbal / Rehman, Aziz-ur

    CAAS. Publishing services by Elsevier B.V Journal of integrative agriculture. 2020 Aug., v. 19, no. 8 p.2035-2043

    2020  

    Abstract: Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in wheat crop every year. Continuous deployment of adult plant resistance (APR) genes in newly developing wheat cultivars is the most judicious ... ...

    Abstract Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in wheat crop every year. Continuous deployment of adult plant resistance (APR) genes in newly developing wheat cultivars is the most judicious strategy to combat this disease. Herein, we dissected the genetics underpinning stripe rust resistance in Pakistani wheat germplasm. An association panel of 94 spring wheat genotypes was phenotyped for two years to score the infestation of stripe rust on each accession and was scanned with 203 polymorphic SSRs. Based on D´ measure, linkage disequilibrium (LD) exhibited between loci distant up to 45 cM. Marker-trait associations (MTAs) were determined using mixed linear model (MLM). Total 31 quantitative trait loci (QTLs) were observed on all 21 wheat chromosomes. Twelve QTLs were newly discovered as well as 19 QTLs and 35 previously reported Yr genes were validated in Pakistani wheat germplasm. The major QTLs were QYr.uaf.2AL and QYr.uaf.3BS (PVE, 11.9%). Dissection of genes from the newly observed QTLs can provide new APR genes to improve genetic resources for APR resistance in wheat crop.
    Keywords Triticum aestivum ; agriculture ; cultivars ; dissection ; genome-wide association study ; germplasm ; linear models ; linkage disequilibrium ; mature plants ; quantitative traits ; spring wheat ; stripe rust ; wheat ; Puccinia striiformis ; LD ; GWAS ; MTA ; PCoA
    Language English
    Dates of publication 2020-08
    Size p. 2035-2043.
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note NAL-AP-2-clean
    ZDB-ID 2660426-7
    ISSN 2095-3119
    ISSN 2095-3119
    DOI 10.1016/S2095-3119(19)62841-8
    Database NAL-Catalogue (AGRICOLA)

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