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Article ; Online: Optical analysis of Sr

Shahab Khan, P / Jamalaiah, B C

Luminescence : the journal of biological and chemical luminescence

2022 Volume 37, Issue 8, Page(s) 1361–1368

Abstract: A series of praseodymium ( ... ...

Abstract	A series of praseodymium (Pr
Language	English
Publishing date	2022-06-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1470995-8
ISSN	1522-7243 ; 1522-7235 ; 1099-1271
ISSN (online)	1522-7243
ISSN	1522-7235 ; 1099-1271
DOI	10.1002/bio.4308
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Article ; Online: Towards sorptive eradication of pharmaceutical micro-pollutant ciprofloxacin from aquatic environment: A comprehensive review.

Khan, Priyanka / Saha, Rajnarayan / Halder, Gopinath

The Science of the total environment

2024 Volume 919, Page(s) 170723

Abstract: Antibiotics are widely prioritized pharmaceuticals frequently adopted in medication for addressing numerous ailments of humans and animals. However, the non-judicious disposal of ciprofloxacin (CIP) with concentration levels exceeding threshold limit in ... ...

Abstract	Antibiotics are widely prioritized pharmaceuticals frequently adopted in medication for addressing numerous ailments of humans and animals. However, the non-judicious disposal of ciprofloxacin (CIP) with concentration levels exceeding threshold limit in an aqueous environment has been the matter of growing concern nowadays. CIP is found in various waterways with appreciable mobility due to its limited decay in solidified form. Hence, the effective eradication strategy of this non-steroidal anti-inflammatory antibiotic from aqueous media is pivotal for preventing the users and the biosphere from their hazardous impacts. Reportedly several customary techniques like reverse osmosis, precipitation, cross-filtration, nano-filtration, ion exchange, microbial remediation, and adsorption have been employed to eliminate CIP from water. Out of them, adsorption is ascertained to be a potential method because of lesser preliminary investment costs, ease of operation, greater efficiency, less energy usage, reduced chemical and biological slurry production, and ready availability of precursor materials. Towards remediation of ciprofloxacin-laden water, plenty of researchers have used different adsorbents. However, the present-day challenge is opting the promising sorbent and its application towards industrial scale-up which is vital to get reviewed. In this article, adsorbents of diverse origins are reviewed in terms of their performances in CIP removal. The review stresses the impact of various factors on sorptive assimilation of CIP, adsorption kinetics, isotherms, mechanism of ionic interaction, contrivances for CIP detection, cost estimation and reusability assessments of adsorbents also that may endorse the next-generation investigators to decide the efficacious, environmental appealing and cost-competitive adsorbents for effective riddance of CIP from wastewater.
MeSH term(s)	Humans ; Ciprofloxacin/analysis ; Anti-Bacterial Agents ; Wastewater ; Adsorption ; Water ; Pharmaceutical Preparations ; Water Pollutants, Chemical/analysis
Chemical Substances	Ciprofloxacin (5E8K9I0O4U) ; Anti-Bacterial Agents ; Wastewater ; Water (059QF0KO0R) ; Pharmaceutical Preparations ; Water Pollutants, Chemical
Language	English
Publishing date	2024-02-08
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.170723
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Zs.A 949: Show issues

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Article ; Online: Imagined speech classification exploiting EEG power spectrum features.

Hossain, Arman / Khan, Protima / Kader, Md Fazlul

Medical & biological engineering & computing

2024

Abstract: Imagined speech recognition has developed as a significant topic of research in the field of brain-computer interfaces. This innovative technique has great promise as a communication tool, providing essential help to those with impairments. An imagined ... ...

Abstract	Imagined speech recognition has developed as a significant topic of research in the field of brain-computer interfaces. This innovative technique has great promise as a communication tool, providing essential help to those with impairments. An imagined speech recognition model is proposed in this paper to identify the ten most frequently used English alphabets (e.g., A, D, E, H, I, N, O, R, S, T) and numerals (e.g., 0 to 9). A novel electroencephalogram (EEG) dataset was created by measuring the brain activity of 30 people while they imagined these alphabets and digits. As part of signal preprocessing, EEG signals are filtered before extracting delta, theta, alpha, and beta band power features. These features are used as input for classification using support vector machines, k-nearest neighbors, and random forest (RF) classifiers. It is identified that the RF classifier outperformed the others in terms of classification accuracy. Classification accuracies of 99.38% and 95.39% were achieved at the coarse-level and fine-level, respectively with the RF classifier. From our study, it is also revealed that the beta frequency band and the frontal lobe of the brain played crucial roles in imagined speech recognition. Furthermore, a comparative analysis against state-of-the-art techniques is conducted to demonstrate the efficacy of our proposed model.
Language	English
Publishing date	2024-04-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	282327-5
ISSN	1741-0444 ; 0025-696X ; 0140-0118
ISSN (online)	1741-0444
ISSN	0025-696X ; 0140-0118
DOI	10.1007/s11517-024-03083-2
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Zs.A 294: Show issues

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Article: Electrophysiological and Visual Parameter Changes in Retinitis Pigmentosa Patients undergoing Autologous Platelet-Rich Plasma Therapy: A Randomized Control Trial.

Khan, Perwez / Khan, Lubna / Kiran, Kumari Kanchan

Journal of current ophthalmology

2024 Volume 35, Issue 3, Page(s) 267–275

Abstract: Purpose: To assess the efficacy of autologous platelet-rich plasma (PRP) injections in suprachoroidal space and subtenon space in cases of retinitis pigmentosa, which is a genetic disease, leading to gradual loss of vision. Till date, no treatment is ... ...

Abstract	Purpose: To assess the efficacy of autologous platelet-rich plasma (PRP) injections in suprachoroidal space and subtenon space in cases of retinitis pigmentosa, which is a genetic disease, leading to gradual loss of vision. Till date, no treatment is available. Methods: Seventy-eight eyes of 39 patients of retinitis pigmentosa having visual acuity ranging from reading of Early Treatment Diabetic Retinopathy Study (ETDRS) chart from 1 m onward to patients who were not able to read the ETDRS chart but whose visual acuity ranged from finger count close to face to <1 m were included in the study. The left and right eyes of each patient were randomized as the intervention eye and control eye. 0.2 mL of autologous PRP was injected in suprachoroidal space and 0.5 mL of PRP was injected in subtenon space of the intervention eye taking aseptic precautions. Injections were repeated at 15-day intervals up to 3 injections. Results: Majority of patients were in the age group of 18-30 years (20 cases) followed by 31-45 years (13 cases) and more than 45 years (6 cases). Intervention eyes showed a statistically significant improvement in visual acuity and multifocal electroretinography (mfERG). Improvement was noted in amplitude density latency and in ring ratio of mfERG. There was a significant improvement in best-corrected visual acuity (BCVA). However, no improvement in mfERG or BCVA was observed in the control group. Conclusions: Gene therapy may be the ultimate cure for retinitis pigmentosa, but it is unaffordable for many patients due to its high cost. PRP may be recognized as a modality to improve vision and stop further deterioration, especially in cases where functional vision is preserved. Negligible treatment costs and affordability will give power to economically disadvantaged patients.
Language	English
Publishing date	2024-03-29
Publishing country	India
Document type	Journal Article
ISSN	2452-2325
ISSN	2452-2325
DOI	10.4103/joco.joco_216_22
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Article ; Online: Acetylation of H3K56 orchestrates UV-responsive chromatin events that generate DNA accessibility during Nucleotide Excision Repair.

Khan, Preeti / Chaudhuri, Ronita Nag

DNA repair

2022 Volume 113, Page(s) 103317

Abstract: Histone modifications have long been related to DNA damage response. Nucleotide excision repair pathway that removes helix-distorting lesions necessitates DNA accessibility through chromatin modifications. Previous studies have linked H3 tail residue ... ...

Abstract	Histone modifications have long been related to DNA damage response. Nucleotide excision repair pathway that removes helix-distorting lesions necessitates DNA accessibility through chromatin modifications. Previous studies have linked H3 tail residue acetylation to UV-induced NER. Here we present evidences that acetylation of H3K56 is crucial for early phases of NER. Using H3K56 mutants K56Q and K56R, which mimic acetylated and unacetylated lysines respectively, we show that recruitment of the repair factor Rad16, a Swi/Snf family member is dependent on H3K56 acetylation. With constitutive H3K56 acetylation, Rad16 recruitment became UV-independent. Furthermore, H3K56 acetylation promoted UV-induced hyperacetylation of H3K9 and H3K14. Importantly, constitutive H3K56 acetylation prominently increased chromatin accessibility. During NER, lack of H3K56 acetylation that effectively aborted H3 tail residue acetylation and Rad16 recruitment, thus failed to impart essential chromatin modulations. The NER-responsive oscillation of chromatin structure observed in wild type, was distinctly eliminated in absence of H3K56 acetylation. In vitro assay with wild type and H3K56 mutant cell extracts further indicated that absence of H3K56 acetylation negatively affected DNA relaxation during NER. Overall, H3K56 acetylation regulates Rad16 redistribution and UV-induced H3 tail residue hyperacetylation, and the resultant modification code promotes chromatin accessibility and recruitment of subsequent repair factors during NER.
Language	English
Publishing date	2022-03-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2071608-4
ISSN	1568-7856 ; 1568-7864
ISSN (online)	1568-7856
ISSN	1568-7864
DOI	10.1016/j.dnarep.2022.103317
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Zs.A 5555: Show issues

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Article ; Online: PACRAT: Pathogen detection with aptamer-detected cascaded recombinase polymerase amplification-in vitro transcription.

Khan, Pavana / Aufdembrink, Lauren / Adamala, Katarzyna P / Engelhart, Aaron

RNA (New York, N.Y.)

2024

Abstract: The SARS-CoV-2 pandemic underscored the need for early, rapid, and widespread pathogen detection tests that are readily accessible. Many existing rapid isothermal detection methods employ the recombinase polymerase amplification (RPA), which exhibits PCR- ...

Abstract	The SARS-CoV-2 pandemic underscored the need for early, rapid, and widespread pathogen detection tests that are readily accessible. Many existing rapid isothermal detection methods employ the recombinase polymerase amplification (RPA), which exhibits PCR-like sensitivity, specificity, and even higher speed. However, coupling RPA to other enzymatic reactions has proven difficult. For the first time, we demonstrate that with tuning of buffer conditions and optimization of reagent concentrations, RPA can be cascaded into an in vitro transcription reaction, enabling detection using fluorescent aptamers in a one-pot reaction. We show that this reaction, which we term PACRAT (Pathogen detection with Aptamer-detected Cascaded Recombinase polymerase Amplification-in vitro Transcription) can be used to detect SARS-CoV-2 with single-copy detection limits and 10-minute detection times. Further demonstrating the utility of our one-pot, cascaded amplification system, we show PACRAT can be employed for multiplexed detection of the pathogens SARS-CoV-2 and E. coli, along with multiplexed detection of two variants of SARS-CoV-2.
Language	English
Publishing date	2024-04-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	1241540-6
ISSN	1469-9001 ; 1355-8382
ISSN (online)	1469-9001
ISSN	1355-8382
DOI	10.1261/rna.079891.123
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Zs.A 4777: Show issues

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Article: Phytochemical investigations and development of ethosomal gel with Brassica oleraceae L. (Brassicaceae) extract: An innovative nano approach towards cosmetic and pharmaceutical industry

Khan, Palwasha / Akhtar, Naveed

Industrial crops and products. 2022 Sept. 01, v. 183

2022

Abstract: The study was aimed to develop and characterize plant extract (Brassica oleraceae) loaded ethosomal gel for the valuation of cosmeceutical applications with a credence that botanical extracts have a greater capability to be employed in the formulation of ...

Abstract	The study was aimed to develop and characterize plant extract (Brassica oleraceae) loaded ethosomal gel for the valuation of cosmeceutical applications with a credence that botanical extracts have a greater capability to be employed in the formulation of advanced pharmaceuticals. Phytochemical profiling via FT-IR analysis, total bioactive contents of different polarity extracts (ethanol, dichloromethane, ethyl-acetate and chloroform) and quantification by using High Performance Liquid Chromatographic (HPLC) assay was carried out. Capabilities against oxidative stress were prodded through 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), reduction potential analyses. Besides this, a correlation amongst total bioactive contents (TPC/TFC) and antioxidant potentials (biological activities) was studied by multi-variate statistical analysis. An augmented ethosomal preparation, after characterization and stability studies was incorporated to the gel matrix of Carbopol-940; Test/ EG (Ethosomal gel) with a Control/NEG (Non-ethosomal gel) formulation. Both formulations were then subjected to in-vitro characterization (organoleptic evaluation, pH, conductivity and rheology) of 90 days. By permeation studies flux (μg/cm²/hr); enhancement ratio was calculated and characterization of release data was done by using different kinetic models. The plant extracts revealed the presence of different functional groups along with 39.6 ± 0.5 mgGAE/g; 26.56 ± 0.4 mgQE/g phenolic and flavonoid contents, respectively; HPLC assay confirmed the existence of Quercetin, Gallic, Caffiec, Benzoic, Syringic and Sinapic Acids with highest antioxidant activity of 87.75% ± 1.18 in hydro-alcoholic fraction. Optimized ethosomal formulation exhibited entrapment efficiency of 68.57 ± 1.25, smallest vesicle size of 228.6 ± 1.17 nm with − 21.3 ± 0.15, zeta potential and a homogeneous size distribution of 0.233 ± 0.02. Significant results were obtained after in-vitro characterization. Both formulations followed Korsmeyer-peppas model with flux 35.499 and 21.731 μg/cm²/hr respectively and enhancement ratio 1.63. Thus, a stable ethosomal loaded gel of Brassica oleraceae extract was successfully developed and characterized for cosmeceutical potential.
Keywords	Brassica oleracea ; antioxidant activity ; antioxidants ; chloroform ; cosmetics ; ethanol ; ethyl acetate ; gels ; high performance liquid chromatography ; methylene chloride ; models ; oxidative stress ; pH ; permeability ; pharmaceutical industry ; plant extracts ; quercetin ; rheology ; sensory evaluation ; statistical analysis ; zeta potential
Language	English
Dates of publication	2022-0901
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1132158-1
ISSN	1872-633X ; 0926-6690
ISSN (online)	1872-633X
ISSN	0926-6690
DOI	10.1016/j.indcrop.2022.114905
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: RNA Polymerase II Dependent Crosstalk between H4K16 Deacetylation and H3K56 Acetylation Promotes Transcription of Constitutively Expressed Genes.

Khan, Preeti / Singha, Priyabrata / Nag Chaudhuri, Ronita

Molecular and cellular biology

2023 Volume 43, Issue 11, Page(s) 596–610

Abstract: Nucleosome dynamics in the coding region of a transcriptionally active locus is critical for understanding how RNA polymerase II progresses through the gene body. Histone acetylation and deacetylation critically influence nucleosome accessibility during ... ...

Abstract	Nucleosome dynamics in the coding region of a transcriptionally active locus is critical for understanding how RNA polymerase II progresses through the gene body. Histone acetylation and deacetylation critically influence nucleosome accessibility during DNA metabolic processes like transcription. Effect of such histone modifications is context and residue dependent. Rather than effect of individual histone residues, the network of modifications of several histone residues in combination generates a chromatin landscape that is conducive for transcription. Here we show that in
MeSH term(s)	Histones/metabolism ; Nucleosomes/metabolism ; RNA Polymerase II/genetics ; RNA Polymerase II/metabolism ; Acetylation ; Chromatin/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism
Chemical Substances	Histones ; Nucleosomes ; RNA Polymerase II (EC 2.7.7.-) ; Chromatin ; Saccharomyces cerevisiae Proteins
Language	English
Publishing date	2023-11-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	779397-2
ISSN	1098-5549 ; 0270-7306
ISSN (online)	1098-5549
ISSN	0270-7306
DOI	10.1080/10985549.2023.2270912
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Zs.A 1666: Show issues

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Article ; Online: The effect of internal displacement due to armed conflict on tuberculosis treatment outcomes in northwest Syria, 2019-2020.

Daif, Abdulselam / Glazik, Rosanna / Checchi, Francesco / Khan, Palwasha

Journal of migration and health

2023 Volume 8, Page(s) 100195

Abstract: Background: Northwest Syria accounts for over 4 million people of whom more than half are internally displaced persons (IDPs). More than 1 million IDPs reside in camps and many more live in settings which are overcrowded and poorly ventilated. ... ...

Abstract	Background: Northwest Syria accounts for over 4 million people of whom more than half are internally displaced persons (IDPs). More than 1 million IDPs reside in camps and many more live in settings which are overcrowded and poorly ventilated. Suboptimal social living conditions leave these populations susceptible to tuberculosis (TB) morbidity and mortality. This study aimed to assess the effect of internal displacement due to armed conflict on the risk of unsuccessful treatment outcomes among TB patients in northwest Syria. Methods: All patients registered to start TB treatment at three centres in northwest Syria between the 1st June 2019 to the 31st December 2020 were included. Unsuccessful TB treatment outcome was defined as a composite outcome combining the WHO TB treatment outcomes of treatment failure, loss to follow-up, and death. We assessed the association between internal displacement and unsuccessful TB treatment outcomes using multivariable logistic regression. We also explored the risk of an unsuccessful treatment outcome by internal displacement setting (camp, village or city). Results: Of the total 737 registered patients included in the analysis, 400 (54%) were documented as internally displaced. The median age of IDPs was 30 (IQR: 21.5-48) and the median age of residents was 34 (IQR:22-50). A significantly higher percentage of those who were IDPs had an unsuccessful treatment outcome compared to residents (40% vs 18%, Conclusion: This is one of few studies which aims to quantify the effect of internal displacement on TB treatment outcomes during times of conflict. Our findings starkly highlight how social determinants contribute to poor TB outcomes and act as a starting point for much needed research on how best to manage TB in humanitarian crisis settings.
Language	English
Publishing date	2023-06-27
Publishing country	England
Document type	Journal Article
ISSN	2666-6235
ISSN (online)	2666-6235
DOI	10.1016/j.jmh.2023.100195
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Article ; Online: QSPR modelling for investigation of different properties of aminoglycoside-derived polymers using 2D descriptors.

Khan, P M / Roy, K

SAR and QSAR in environmental research

2021 Volume 32, Issue 7, Page(s) 595–614

Abstract: The quantitative structure-property relationship (QSPR) method is commonly used to predict different physicochemical characteristics of interest of chemical compounds with an objective to accelerate the process of design and development of novel chemical ...

Abstract	The quantitative structure-property relationship (QSPR) method is commonly used to predict different physicochemical characteristics of interest of chemical compounds with an objective to accelerate the process of design and development of novel chemical compounds in the biotechnology and healthcare industries. In the present report, we have employed a QSPR approach to predict the different properties of the aminoglycoside-derived polymers (i.e. polymer DNA binding and aminoglycoside-derived polymers mediated transgene expression). The final QSPR models were obtained using the partial least squares (PLS) regression approach using only specific categories of two-dimensional descriptors and subsequently evaluated considering different internationally accepted validation metrics. The proposed models are robust and non-random, demonstrating excellent predictive ability using test set compounds. We have also developed different kinds of consensus models using several validated individual models to improve the prediction quality for external set compounds. The present findings provide new insight for exploring the design of an aminoglycoside-derived polymer library based on different identified physicochemical properties as well as predict their property before their synthesis.
MeSH term(s)	Aminoglycosides/chemistry ; DNA/metabolism ; Gene Expression/drug effects ; Least-Squares Analysis ; Polymers/chemistry ; Polymers/metabolism ; Polymers/pharmacology ; Quantitative Structure-Activity Relationship ; Transgenes
Chemical Substances	Aminoglycosides ; Polymers ; DNA (9007-49-2)
Language	English
Publishing date	2021-06-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2023559-8
ISSN	1029-046X ; 1062-936X
ISSN (online)	1029-046X
ISSN	1062-936X
DOI	10.1080/1062936X.2021.1939150
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