Artikel: Structure-biological activity relationships of myeloperoxidase to effect on platelet activation
Archives of biochemistry and biophysics. 2022 July 11,
2022
Abstract: Myeloperoxidase (MPO), an oxidant-producing enzyme of neutrophils, has been shown to prime platelet activity promoting immunothrombosis. Native MPO is a homodimer, consisting of two identical protomers (monomer) connected by a single disulfide bond. But ... ...
| Abstract | Myeloperoxidase (MPO), an oxidant-producing enzyme of neutrophils, has been shown to prime platelet activity promoting immunothrombosis. Native MPO is a homodimer, consisting of two identical protomers (monomer) connected by a single disulfide bond. But in inflammatory foci, MPO can be found both in the form of a monomer and in the form of a dimer. Beside MPO can also be in complexes with other molecules and be modified by oxidants, which ultimately affect its physicochemical properties and functions. Here we compared the effects of various forms of MPO as well as MPO in complex with ceruloplasmin (CP), a physiological inhibitor of MPO, on the platelet activity. Monomeric MPO (hemi-MPO) was obtained by treating the dimeric MPO by reductive alkylation. MPO was modified with HOCl in a molar ratio of 1:100 (MPO-HOCl). Using surface-enhanced Raman scattering (SERS) spectroscopy we showed that peaks at about 510 and 526 cm⁻¹ corresponded to disulfide bond was recognizable in the SERS-spectra of dimeric MPO, absent in the spectrum of hemi-MPO and less intense in the spectra of MPO-HOCl, which indicates the partial decomposition of dimeric MPO with a disulfide bond cleavage under the HOCl modification. It was shown hemi-MPO to a lesser extent than dimeric MPO bound to platelets and enhanced their agonist-induced aggregation and platelet-neutrophil aggregate formation. MPO modified by HOCl and MPO in complex with CP did not bind to platelets and have no effect on platelet activity. Thus, the modification of MPO by HOCl, its presence in monomeric form as well as in complex with CP reduces MPO effect on platelet function and consequently decreases the risk of thrombosis in inflammatory foci. |
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| Schlagwörter | alkylation ; biophysics ; cleavage (chemistry) ; disulfide bonds ; ferroxidase ; myeloperoxidase ; neutrophils ; platelet activation ; protein subunits ; risk ; thrombosis |
| Sprache | Englisch |
| Erscheinungsverlauf | 2022-0711 |
| Erscheinungsort | Elsevier Inc. |
| Dokumenttyp | Artikel |
| Anmerkung | Pre-press version |
| ZDB-ID | 523-x |
| ISSN | 1096-0384 ; 0003-9861 |
| ISSN (online) | 1096-0384 |
| ISSN | 0003-9861 |
| DOI | 10.1016/j.abb.2022.109353 |
| Datenquelle | NAL Katalog (AGRICOLA) |
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