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  1. Article ; Online: Case report: Amniotic fluid-derived mesenchymal stem cell treatment in a dog with a spinal cord injury.

    Kim, Eun Young / Kil, Tae Young / Kim, Min Kyu

    Veterinary and animal science

    2023  Volume 22, Page(s) 100318

    Abstract: Spinal Cord Injury (SCI) refers to complete or incomplete damage to the spinal cord, which comprises the central nervous system. SCI in dogs, like humans, is mostly caused by external trauma, and the degree of impact is dependent on the location of the ... ...

    Abstract Spinal Cord Injury (SCI) refers to complete or incomplete damage to the spinal cord, which comprises the central nervous system. SCI in dogs, like humans, is mostly caused by external trauma, and the degree of impact is dependent on the location of the injury in the spine. Stem cell therapy is a promising avenue for SCI research. In this report, we investigate the therapeutic potential of amniotic fluid-derived mesenchymal stem cells (AF-MSCs) in dogs with spinal cord injuries. A 2-year-old male beagle dog presented with sensory and motor incomplete symptoms resulting in an inability to control the legs, hips, and genitourinary system due to an injury in the lumbar region of the spinal cord. In addition to the administration of surgical decompression, AF-MSCs were directly injected into the damaged spinal tissue. Approximately 15-16 weeks after stem cell transplantation, the dog's hind limb movement improved, and spinal cord regeneration was confirmed through magnetic resonance imaging (MRI). Eventually, the dog was able to walk independently, although not perfectly. In conclusion, AF-MSC-based stem cell transplantation may be beneficial for SCIs.
    Language English
    Publishing date 2023-10-30
    Publishing country Netherlands
    Document type Case Reports
    ISSN 2451-943X
    ISSN (online) 2451-943X
    DOI 10.1016/j.vas.2023.100318
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Evaluation of stability and safety of equine mesenchymal stem cells derived from amniotic fluid for clinical application.

    Kim, Eun Young / Lee, Eun Ji / Kim, Ryoung Eun / Kil, Tae Young / Kim, Min Kyu

    Frontiers in veterinary science

    2024  Volume 11, Page(s) 1330009

    Abstract: Amniotic fluid mesenchymal stem cells (AF-MSCs), which can be obtained from fetal tissue, reportedly have self-renewal capacity and multi-lineage differentiation potential. The aim of this study was to identify the biological characteristics of AF-MSCs ... ...

    Abstract Amniotic fluid mesenchymal stem cells (AF-MSCs), which can be obtained from fetal tissue, reportedly have self-renewal capacity and multi-lineage differentiation potential. The aim of this study was to identify the biological characteristics of AF-MSCs and evaluate their stability and safety in long-term culture. To confirm the biological characteristics of AF-MSCs, morphology, proliferation capacity, karyotype, differentiation capacity, gene expression level, and immunophenotype were analyzed after isolating AF-MSCs from equine amniotic fluid. AF-MSCs were differentiated into adipocytes, chondrocytes, and osteocytes. Immunophenotype analyses revealed expression levels of ≥95% and ≤ 2% of cells for a positive and negative marker, respectively. Analysis of the MSCs relative gene expression levels of AF-MSCs was approximately at least twice that of the control. The endotoxin level was measured to verify the safety of AF-MSCs and was found to be less than the standard value of 0.5 EU/ml. AF-MSCs were cultured for a long time without any evidence of abnormalities in morphology, proliferation ability, and karyotype. These results suggest that amniotic fluid is a competent source for acquiring equine MSCs and that it is valuable as a cell therapy due to its high stability.
    Language English
    Publishing date 2024-02-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2024.1330009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Generation of genome-edited dogs by somatic cell nuclear transfer.

    Kim, Dong-Ern / Lee, Ji-Hye / Ji, Kuk-Bin / Park, Kang-Sun / Kil, Tae-Young / Koo, Okjae / Kim, Min-Kyu

    BMC biotechnology

    2022  Volume 22, Issue 1, Page(s) 19

    Abstract: Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.: Results: ...

    Abstract Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.
    Results: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed.
    Conclusion: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.
    MeSH term(s) Animals ; Animals, Genetically Modified ; CRISPR-Cas Systems/genetics ; Cloning, Organism ; Dogs ; Gene Editing ; Nuclear Transfer Techniques
    Language English
    Publishing date 2022-07-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2052746-9
    ISSN 1472-6750 ; 1472-6750
    ISSN (online) 1472-6750
    ISSN 1472-6750
    DOI 10.1186/s12896-022-00749-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Generation of genome-edited dogs by somatic cell nuclear transfer

    Kim, Dong-Ern / Lee, Ji-Hye / Ji, Kuk-Bin / Park, Kang-Sun / Kil, Tae-Young / Koo, Okjae / Kim, Min-Kyu

    BMC Biotechnol. 2022 Dec., v. 22, no. 1 p.19-19

    2022  

    Abstract: BACKGROUND: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. RESULTS: We ... ...

    Abstract BACKGROUND: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. RESULTS: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed. CONCLUSION: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.
    Keywords CRISPR-Cas systems ; antibiotics ; dogs ; fibroblasts ; gene editing ; loci ; purebreds ; somatic cells ; transfection
    Language English
    Dates of publication 2022-12
    Size p. 19.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2052746-9
    ISSN 1472-6750
    ISSN 1472-6750
    DOI 10.1186/s12896-022-00749-3
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Toxicity of the recombinant human hyaluronidase ALT-BC4 on embryonic development.

    Lee, Ji Hye / Yoo, Miyoun / Lee, Sang Mee / Park, Soon-Jae / Kil, Tae Young / Kim, Min Kyu

    Journal of animal science and technology

    2021  Volume 63, Issue 2, Page(s) 272–280

    Abstract: Cumulus-oocyte complexes (COCs), which contain immature oocytes, are ... ...

    Abstract Cumulus-oocyte complexes (COCs), which contain immature oocytes, are matured
    Language English
    Publishing date 2021-03-31
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2775231-8
    ISSN 2055-0391
    ISSN 2055-0391
    DOI 10.5187/jast.2021.e34
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Application of the modified handmade cloning technique to pigs.

    Lee, Eun Ji / Ji, Kuk Bin / Lee, Ji Hye / Oh, Hyun Ju / Kil, Tae Young / Kim, Min Kyu

    Journal of animal science and technology

    2021  Volume 63, Issue 2, Page(s) 281–294

    Abstract: Although somatic cell nuclear transfer (SCNT) is frequently employed to produce cloned animals in laboratories, this technique is expensive and inefficient. Therefore, the handmade cloning (HMC) technique has been suggested to simplify and advance the ... ...

    Abstract Although somatic cell nuclear transfer (SCNT) is frequently employed to produce cloned animals in laboratories, this technique is expensive and inefficient. Therefore, the handmade cloning (HMC) technique has been suggested to simplify and advance the cloning process, however, HMC wastes many oocytes and leads to mitochondrial heteroplasmy. To solve these problems, we propose a modified handmade cloning (mHMC) technique that uses simple laboratory equipment, i.e., a Pasteur pipette and an alcohol lamp, applying it to porcine embryo cloning. To validate the application of mHMC to pig cloning, embryos produced through SCNT and mHMC are compared using multiple methods, such as enucleation efficiency, oxidative stress, embryo developmental competence, and gene expression. The results show no significant differences between techniques except in the enucleation efficiency. The 8-cell and 16-cell embryo developmental competence and Oct4 expression levels exhibit significant differences. However, the blastocyst rate is not significantly different between mHMC and SCNT. This study verifies that cloned embryos derived from the two techniques exhibit similar generation and developmental competence. Thus, we suggest that mHMC could replace SCNT for simpler and cheaper porcine cloning.
    Language English
    Publishing date 2021-03-31
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2775231-8
    ISSN 2055-0391
    ISSN 2055-0391
    DOI 10.5187/jast.2021.e41
    Database MEDical Literature Analysis and Retrieval System OnLINE

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