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Article ; Online: Potential applications of mesenchymal stem cells and their derived exosomes in regenerative medicine.

Adelipour, Maryam / Lubman, David M / Kim, Jeongkwon

2023 Volume 23, Issue 6, Page(s) 491–507

Abstract: Introduction: Regenerative medicine involves the replacement of damaged cells, tissues, or organs to restore normal function. Mesenchymal stem cells (MSCs) and exosomes secreted by MSCs have unique advantages that make them a suitable candidate in the ... ...

Abstract	Introduction: Regenerative medicine involves the replacement of damaged cells, tissues, or organs to restore normal function. Mesenchymal stem cells (MSCs) and exosomes secreted by MSCs have unique advantages that make them a suitable candidate in the field of regenerative medicine. Areas covered: This article provides a comprehensive overview of regenerative medicine, focusing on the use of MSCs and their exosomes as potential therapies for replacing damaged cells, tissues, or organs. This article discusses the distinct advantages of both MSCs and their secreted exosomes, including their immunomodulatory effects, lack of immunogenicity, and recruitment to damaged areas. While both MSCs and exosomes have these advantages, MSCs also have the unique ability to self-renew and differentiate. This article also assesses the current challenges associated with the application of MSCs and their secreted exosomes in therapy. We have reviewed proposed solutions for improving MSC or exosome therapy, including ex-vivo preconditioning strategies, genetic modification, and encapsulation. Literature search was conducted using Google Scholar and PubMed databases. Expert opinion: Providing insight into the future development of MSC and exosome-based therapies and to encourage the scientific community to focus on the identified gaps, develop appropriate guidelines, and enhance the clinical application of these therapies.
MeSH term(s)	Humans ; Regenerative Medicine ; Exosomes ; Mesenchymal Stem Cells
Language	English
Publishing date	2023-05-09
Publishing country	England
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2052501-1
ISSN	1744-7682 ; 1471-2598
ISSN (online)	1744-7682
ISSN	1471-2598
DOI	10.1080/14712598.2023.2211203
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Article ; Online: Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars.

Jalaludin, Iqbal / Kim, Jeongkwon

Food chemistry

2021 Volume 365, Page(s) 130514

Abstract: Refractive index (RI) detection is the standard approach for quantitatively detecting sugars via high-performance liquid chromatography (HPLC), while ultraviolet (UV) absorbance detection is the most commonly used detection method for general HPLC ... ...

Abstract	Refractive index (RI) detection is the standard approach for quantitatively detecting sugars via high-performance liquid chromatography (HPLC), while ultraviolet (UV) absorbance detection is the most commonly used detection method for general HPLC analysis. We compared the two detection approaches of UV and RI in the HPLC analysis of small sugars to investigate whether UV detection could be an alternative method to RI detection. UV detection was performed using a photodiode array scanning from 190 to 400 nm. We obtained comparable limit of detection (LOD) results for RI and UV detection in the HPLC analysis of monosaccharides, while HPLC-RI provided better LOD results than HPLC-UV in disaccharide analysis. Both HPLC-RI and HPLC-UV methods were applied to analyze a real honey sample, and similar results were obtained in terms of precision and recovery. The study conclusively shows that the UV-based HPLC analysis of sugars offers a sufficient alternative to RI-based HPLC analysis.
MeSH term(s)	Chromatography, High Pressure Liquid ; Limit of Detection ; Refractometry ; Spectrophotometry, Ultraviolet ; Sugars
Chemical Substances	Sugars
Language	English
Publishing date	2021-07-01
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2021.130514
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Article ; Online: Analysis of Self-Assembled Micelle Inhibitory RNA (SAMiRNA) Drug Using Ion-Pairing Reversed-Phase Liquid Chromatography Combined with Mass Spectrometry.

Hwang, Hyojin / Park, Kyu H / Kwon, Ju-Lee / Park, Han-Oh / Kim, Jeongkwon

Journal of the American Society for Mass Spectrometry

2024

Abstract: Small interfering RNA (siRNA) is known for its ability to silence the expression of specific genes, demonstrating its promising potential as a therapeutic approach. Self-assembled micelle inhibitory RNA (SAMiRNA) is an oligonucleotide duplex developed to ...

Abstract	Small interfering RNA (siRNA) is known for its ability to silence the expression of specific genes, demonstrating its promising potential as a therapeutic approach. Self-assembled micelle inhibitory RNA (SAMiRNA) is an oligonucleotide duplex developed to overcome the in vivo delivery limitations of siRNA. SAMiRNA has hydrophilic and hydrophobic groups at both ends of a sense strand, forming a spherical nanostructure that enhances the in vivo delivery efficiency. Ion-pairing reversed-phase liquid chromatography (IP-RPLC) is the most commonly used method for the analysis of oligonucleotides. Since SAMiRNA is heavily chemically modified, the behavior of SAMiRNA in IP-RPLC combined with mass spectrometry (MS) is anticipated to differ from that of the conventional siRNA drug. The current investigation using IP-RPLC-MS revealed that a distinct duplex peak along with two minor separate strands of antisense and sense was observed at column temperatures below 35 °C in the IP-RPLC system with a 100 mM ammonium bicarbonate buffer system. At column temperatures higher than 35 °C, however, two fully denatured single strands were observed. The mass spectrum from the chromatographic peak of the SAMiRNA duplex contained signals from the duplex, the antisense, and the sense, probably due to duplex denaturation during the MS ionization process. The current comprehensive analysis results will make a substantial contribution to the future application of IP-RPLC-MS in the analysis of SAMiRNA.
Language	English
Publishing date	2024-04-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	1073671-2
ISSN	1879-1123 ; 1044-0305
ISSN (online)	1879-1123
ISSN	1044-0305
DOI	10.1021/jasms.4c00081
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Article: Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars

Jalaludin, Iqbal / Kim, Jeongkwon

Food chemistry. 2021 Dec. 15, v. 365

2021

Abstract	Refractive index (RI) detection is the standard approach for quantitatively detecting sugars via high-performance liquid chromatography (HPLC), while ultraviolet (UV) absorbance detection is the most commonly used detection method for general HPLC analysis. We compared the two detection approaches of UV and RI in the HPLC analysis of small sugars to investigate whether UV detection could be an alternative method to RI detection. UV detection was performed using a photodiode array scanning from 190 to 400 nm. We obtained comparable limit of detection (LOD) results for RI and UV detection in the HPLC analysis of monosaccharides, while HPLC-RI provided better LOD results than HPLC-UV in disaccharide analysis. Both HPLC-RI and HPLC-UV methods were applied to analyze a real honey sample, and similar results were obtained in terms of precision and recovery. The study conclusively shows that the UV-based HPLC analysis of sugars offers a sufficient alternative to RI-based HPLC analysis.
Keywords	absorbance ; detection limit ; food chemistry ; high performance liquid chromatography ; honey ; refractive index
Language	English
Dates of publication	2021-1215
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2021.130514
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Article ; Online: Ultraviolet Light-Assisted Decontamination of Chemical Warfare Agent Simulant 2-Chloroethyl Phenyl Sulfide on Metal-Loaded TiO

Jang, Hye Ji / Yun, Gaeun / Shim, Huieun / Hwang, Seon Young / Kim, So Young / Kim, Jeongkwon / Jung, Heesoo / Khan, Mohammad Mansoob / Sohn, Youngku

ChemistryOpen

2024 , Page(s) e202300246

Abstract: The application of ultraviolet (UV) light for the decontamination of chemical warfare agents (CWAs) has gained recognition as an effective method, especially for treating hard-to-reach areas where wet chemical methods are impractical. In this study, ... ...

Abstract	The application of ultraviolet (UV) light for the decontamination of chemical warfare agents (CWAs) has gained recognition as an effective method, especially for treating hard-to-reach areas where wet chemical methods are impractical. In this study, TiO
Language	English
Publishing date	2024-02-20
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2655605-4
ISSN	2191-1363 ; 2191-1363
ISSN (online)	2191-1363
ISSN	2191-1363
DOI	10.1002/open.202300246
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Article ; Online: A guide to mass spectrometric analysis of extracellular vesicle proteins for biomarker discovery.

Jalaludin, Iqbal / Lubman, David M / Kim, Jeongkwon

Mass spectrometry reviews

2021 Volume 42, Issue 2, Page(s) 844–872

Abstract: Exosomes (small extracellular vesicles) in living organisms play an important role in processes such as cell proliferation or intercellular communication. Recently, exosomes have been extensively investigated for biomarker discoveries for various ... ...

Abstract	Exosomes (small extracellular vesicles) in living organisms play an important role in processes such as cell proliferation or intercellular communication. Recently, exosomes have been extensively investigated for biomarker discoveries for various diseases. An important aspect of exosome analysis involves the development of enrichment methods that have been introduced for successful isolation of exosomes. These methods include ultracentrifugation, size exclusion chromatography, polyethylene glycol-based precipitation, immunoaffinity-based enrichment, ultrafiltration, and asymmetric flow field-flow fractionation among others. To confirm the presence of exosomes, various characterization methods have been utilized such as Western blot analysis, atomic force microscopy, electron microscopy, optical methods, zeta potential, visual inspection, and mass spectrometry. Recent advances in high-resolution separations, high-performance mass spectrometry and comprehensive proteome databases have all contributed to the successful analysis of exosomes from patient samples. Herein we review various exosome enrichment methods, characterization methods, and recent trends of exosome investigations using mass spectrometry-based approaches for biomarker discovery.
MeSH term(s)	Humans ; Mass Spectrometry/methods ; Exosomes/chemistry ; Proteome/analysis ; Ultracentrifugation ; Biomarkers/analysis
Chemical Substances	Proteome ; Biomarkers
Language	English
Publishing date	2021-11-08
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	1491946-1
ISSN	1098-2787 ; 0277-7037
ISSN (online)	1098-2787
ISSN	0277-7037
DOI	10.1002/mas.21749
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Article ; Online: Accurate determination of 11 representative phthalates and di(2-ethylhexyl) terephthalate in polyvinyl chloride using isotope dilution-gas chromatography/mass spectrometry.

Min, Sang-Won / Lim, Dong Kyu / Lee, Sunyoung / Kim, Jeongkwon / Baek, Song-Yee

Journal of chromatography. A

2023 Volume 1711, Page(s) 464454

Abstract: Phthalates are mainly used as plasticizers in polyvinyl chloride (PVC). However, prolonged exposure to phthalates poses considerable risks to human health. Consequently, the utilization of phthalates in consumer products is subject to regulations, with a ...

Abstract	Phthalates are mainly used as plasticizers in polyvinyl chloride (PVC). However, prolonged exposure to phthalates poses considerable risks to human health. Consequently, the utilization of phthalates in consumer products is subject to regulations, with a defined threshold of 0.1 %. In this study, we developed an accurate and simultaneous method for determination of 11 representative phthalates and a non-phthalate plasticizer (di(2-ethylhexyl) terephthalate, DEHT) in PVC as a higher-order reference method. Homogeneously prepared PVC samples, each containing approximately 0.1 % of the target plasticizer compounds, were analyzed using gas chromatography-mass spectrometry (GC-MS) with deuterium-labeled phthalates and DEHT. The developed method could effectively separate and quantify all target plasticizers without interference with each other and potential overlap between the isomeric forms of phthalates, di-isodecyl phthalate, and di-isononyl phthalate. The developed method has high-order metrological quality, exhibiting exceptional selectivity, accuracy, repeatability (≤ 2.17 %), reproducibility (≤ 2.16 %), and relative expanded uncertainty (≤ 5.6 %). This analytical method is thus suitable for accurately assessing the target plasticizer levels in PVC products for ensuring compliance with the established 0.1 % threshold. This method was successfully applied to quantify twelve distinct plasticizers in PVC products obtained from the Korean market, validating its effectiveness and reliability in real-world scenarios.
MeSH term(s)	Humans ; Plasticizers/analysis ; Polyvinyl Chloride/chemistry ; Reproducibility of Results ; Phthalic Acids/analysis ; Mass Spectrometry ; Gas Chromatography-Mass Spectrometry/methods ; Isotopes ; Diethylhexyl Phthalate/analysis
Chemical Substances	phthalic acid (6O7F7IX66E) ; terephthalic acid (6S7NKZ40BQ) ; Plasticizers ; Polyvinyl Chloride (9002-86-2) ; Phthalic Acids ; Isotopes ; Diethylhexyl Phthalate (C42K0PH13C)
Language	English
Publishing date	2023-10-16
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2023.464454
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Article ; Online: Identification of geographical origins of soybean pastes using headspace gas chromatography-mass spectrometry by selecting sample-descriptive components with an Incremental Association Markov Blanket.

Jeong, Seongsoo / Kwon, Dokyung / Lim, Jina / Jang, Hanbyeol / Kim, Jeongkwon / Chung, Hoeil

Food research international (Ottawa, Ont.)

2023 Volume 174, Issue Pt 1, Page(s) 113492

Abstract: The identification of geographical origins of soybean pastes using headspace gas chromatography-mass spectrometry was attempted in this study. Since soybean paste was odor-rich, 36 components were identified in the imported and domestic soybean samples. ... ...

Abstract	The identification of geographical origins of soybean pastes using headspace gas chromatography-mass spectrometry was attempted in this study. Since soybean paste was odor-rich, 36 components were identified in the imported and domestic soybean samples. t-Test, variable importance in projection (VIP), and Incremental Association Markov Blanket (IAMB) were employed to select proper components that could effectively discriminate the two sample groups. The discrimination accuracies were below 87.3 % when all 36 components were fed for either LDA, k-NN, or SVM. When the five t-test-selected components or six VIP score-selected components were employed, the accuracies improved to 95.2-96.2 %. The IAMB selected three different components were 3-methylbutanal, 4-methylnonane, and 2,3-pentanedione, and the correlations among their peak areas were not significant. This suggests that these three components were independently relevant for the discrimination. The accuracy obtained using these three components was superior, 97.7 %, as undescriptive and/or redundant components for the discrimination were excluded.
MeSH term(s)	Gas Chromatography-Mass Spectrometry/methods ; Glycine max ; Ketones ; Geography ; Odorants
Chemical Substances	Ketones
Language	English
Publishing date	2023-09-23
Publishing country	Canada
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1111695-x
ISSN	1873-7145 ; 0963-9969
ISSN (online)	1873-7145
ISSN	0963-9969
DOI	10.1016/j.foodres.2023.113492
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Article ; Online: Detection of multiply charged protein ions using matrix-assisted laser desorption/ionization mass spectrometry and a force-dried droplet method with a 2-nitrophloroglucinol matrix.

Yun, Kangseok / Jalaludin, Iqbal / Jung, Shinhee / Jang, Kyoung-Soon / Kim, Jeongkwon

The Analyst

2022 Volume 147, Issue 3, Page(s) 505–515

Abstract: Conventional dried droplet (DD) methods show poor reproducibility in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) due to the frequent induction of a heterogeneous sample distribution. Recently, a forced dried droplet (FDD) ... ...

Abstract	Conventional dried droplet (DD) methods show poor reproducibility in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) due to the frequent induction of a heterogeneous sample distribution. Recently, a forced dried droplet (FDD) sample preparation method was introduced to form homogeneous samples; this method improves the reproducibility of MALDI-MS analysis and generates highly multiply charged ions compared to DD methods. The FDD method utilizes secondary nucleation to generate a homogeneous sample distribution by applying an external force such as fluid shear stress by stirring the sample using a micropipette tip. In this study, a 2-nitrophloroglucinol (2-NPG) matrix was used for the DD and FDD sample preparation methods, and the charge state and homogeneity were compared by detecting multiply charged ions of proteins including cytochrome
MeSH term(s)	Ions ; Lasers ; Phloroglucinol/analogs & derivatives ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical Substances	2-nitrophloroglucinol ; Ions ; Phloroglucinol (DHD7FFG6YS)
Language	English
Publishing date	2022-01-31
Publishing country	England
Document type	Journal Article
ZDB-ID	210747-8
ISSN	1364-5528 ; 0003-2654
ISSN (online)	1364-5528
ISSN	0003-2654
DOI	10.1039/d1an02114a
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Article ; Online: Matrix-assisted laser desorption/ionization-Fourier-transform ion cyclotron resonance-mass spectrometry analysis of exosomal lipids from human serum.

Jalaludin, Iqbal / Nguyen, Huu-Quang / Jang, Kyoung-Soon / Lee, Jaebeom / Lubman, David M / Kim, Jeongkwon

Rapid communications in mass spectrometry : RCM

2022 Volume 37, Issue 2, Page(s) e9427

Abstract: Rationale: Exosomes contain biomarkers such as proteins and lipids that help in understanding normal physiology and diseases. Lipids, in particular, are infrequently studied using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) ...

Abstract	Rationale: Exosomes contain biomarkers such as proteins and lipids that help in understanding normal physiology and diseases. Lipids, in particular, are infrequently studied using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) for biomarker discovery. In this study, MALDI was equipped with a high-resolution MS to investigate exosomal lipids from human serum. Methods: Exosomal lipids were profiled using MALDI with Fourier-transform ion cyclotron resonance (FTICR)-MS. Four matrices (i.e., α-cyano-4-hydroxycinnamic acid [CHCA], 2,5-dihydroxybenzoic acid, sinapinic acid, and graphene oxide [GO]) and three sample preparation methods (i.e., dried droplet, thin layer, and two layer) were compared for the number of lipid species detected and the relative abundance of each lipid from human serum and human serum exosomes. Results: In sum, 172 and 89 lipid species were identified from human serum and human serum exosomes, respectively, using all the methods. The highest number of exosome lipid species, 69, was detected using the CHCA matrix, whereas only 8 exosome lipid species were identified using the GO matrix. Among the identified lipid species, phosphatidylcholine was identified most frequently, probably due to the use of a positive ion mode. Conclusions: Exosomes and human serum showed comparable lipid profiles as determined using MALDI-FTICR-MS. These findings provide a new perspective on exosomal lipidomics analysis and may serve as a foundation for future lipidomics-based biomarker research using MALDI-FTICR-MS.
MeSH term(s)	Humans ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Proteins ; Lipids/analysis ; Lasers
Chemical Substances	Proteins ; graphene oxide ; Lipids
Language	English
Publishing date	2022-10-30
Publishing country	England
Document type	Journal Article
ZDB-ID	58731-x
ISSN	1097-0231 ; 0951-4198
ISSN (online)	1097-0231
ISSN	0951-4198
DOI	10.1002/rcm.9427
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