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  1. Article ; Online: Production of the Rare Ginsenoside Rh2-MIX (20(

    Song, Bong-Kyu / Kim, Kyeng Min / Choi, Kang-Duk / Im, Wan-Taek

    Journal of microbiology and biotechnology

    2017  Volume 27, Issue 7, Page(s) 1233–1241

    Abstract: The ginsenoside Rh2 has strong anti-cancer, anti-inflammatory, and anti-diabetic effects. However, the application of ginsenoside Rh2 is restricted because of the small amounts found in Korean white and red ginsengs. To enhance the production of ... ...

    Abstract The ginsenoside Rh2 has strong anti-cancer, anti-inflammatory, and anti-diabetic effects. However, the application of ginsenoside Rh2 is restricted because of the small amounts found in Korean white and red ginsengs. To enhance the production of ginsenoside Rh2-MIX (comprising 20(
    Language English
    Publishing date 2017-07-28
    Publishing country Korea (South)
    Document type Journal Article
    ISSN 1738-8872
    ISSN (online) 1738-8872
    DOI 10.4014/jmb.1701.01077
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Sphingosinicella ginsenosidimutans sp. nov., with ginsenoside converting activity.

    Kim, Jin-Kwang / Kang, Myung-Suk / Park, Sung Chul / Kim, Kyeng-Min / Choi, Kangduk / Yoon, Min-Ho / Im, Wan-Taek

    Journal of microbiology (Seoul, Korea)

    2015  Volume 53, Issue 7, Page(s) 435–441

    Abstract: The Gram-reaction-negative, strictly aerobic, non-motile, nonspore-forming, and rod-shaped bacterial strain designated BS11(T) was isolated from the compost and its taxonomic position was investigated by using a polyphasic approach. Strain BS11(T) grew ... ...

    Abstract The Gram-reaction-negative, strictly aerobic, non-motile, nonspore-forming, and rod-shaped bacterial strain designated BS11(T) was isolated from the compost and its taxonomic position was investigated by using a polyphasic approach. Strain BS11(T) grew optimally at 30-37°C and at pH 7.0 in the absence of NaCl on nutrient agar. Strain BS11(T) displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to Rd. On the basis of 16S rRNA gene sequence similarity, strain BS11(T) was shown to belong to the family Sphingomonadaceae and was related to Sphingosinicella vermicomposti YC7378(T) (96.3% sequence similarity), S. xenopeptidilytica 3-2W4(T) (96.2%), S. microcystinivorans Y2(T) (96.1%), and S. soli KSL-125(T) (95.9%). The G+C content of the genomic DNA was 64.9%. The major menaquinone was Q-10 and the major fatty acids were summed feature 7 (comprising C18:1 ω7c/ω9t/ω12t; 40.6%), C16:0 (22.5%), C17:1 ω6c (13.7%) and C17:0 (9.1%). DNA and chemotaxonomic data supported the affiliation of strain BS11(T) to the genus Sphingosinicella. Strain BS11(T) could be differentiated genotypically and phenotypically from the recognized species of the genus Sphingosinicella. The novel isolate therefore represents a novel species, for which the name Sphingosinicella ginsenosidimutans sp. nov. is proposed, with the type strain BS11(T) (=KACC 16619T =JCM 18201(T)).
    MeSH term(s) Bacterial Typing Techniques ; Base Composition/genetics ; DNA, Ribosomal/genetics ; Fatty Acids/chemistry ; Ginsenosides/metabolism ; Phenotype ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Soil ; Soil Microbiology ; Sphingomonadaceae/classification ; Sphingomonadaceae/genetics ; Sphingomonadaceae/isolation & purification ; Sphingomonadaceae/metabolism ; Vitamin K 2/analysis ; beta-Glucosidase/metabolism
    Chemical Substances DNA, Ribosomal ; Fatty Acids ; Ginsenosides ; RNA, Ribosomal, 16S ; Soil ; Vitamin K 2 (11032-49-8) ; ginsenoside Rb1 (7413S0WMH6) ; beta-Glucosidase (EC 3.2.1.21)
    Language English
    Publishing date 2015-07
    Publishing country Korea (South)
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2012399-1
    ISSN 1976-3794 ; 1225-8873
    ISSN (online) 1976-3794
    ISSN 1225-8873
    DOI 10.1007/s12275-015-5087-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article: Sphingosinicella ginsenosidimutans sp. nov., with ginsenoside converting activity

    Kim, Jin-Kwang / Kang, Myung-Suk / Park, Sung Chul / Kim, Kyeng-Min / Choi, Kangduk / Yoon, Min-Ho / Im, Wan-Taek

    journal of microbiology

    Volume v. 53,, Issue no. 7

    Abstract: The Gram-reaction-negative, strictly aerobic, non-motile, nonspore-forming, and rod-shaped bacterial strain designated BS11ᵀ was isolated from the compost and its taxonomic position was investigated by using a polyphasic approach. Strain BS11ᵀ grew ... ...

    Abstract The Gram-reaction-negative, strictly aerobic, non-motile, nonspore-forming, and rod-shaped bacterial strain designated BS11ᵀ was isolated from the compost and its taxonomic position was investigated by using a polyphasic approach. Strain BS11ᵀ grew optimally at 30–37°C and at pH 7.0 in the absence of NaCl on nutrient agar. Strain BS11ᵀ displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to Rd. On the basis of 16S rRNA gene sequence similarity, strain BS11ᵀ was shown to belong to the family Sphingomonadaceae and was related to Sphingosinicella vermicomposti YC7378ᵀ (96.3% sequence similarity), S. xenopeptidilytica 3–2W4ᵀ (96.2%), S. microcystinivorans Y2ᵀ (96.1%), and S. soli KSL-125ᵀ (95.9%). The G+C content of the genomic DNA was 64.9%. The major menaquinone was Q-10 and the major fatty acids were summed feature 7 (comprising C₁₈:₁ ω7c/ω9t/ω12t; 40.6%), C₁₆:₀ (22.5%), C₁₇:₁ ω6c (13.7%) and C₁₇:₀ (9.1%). DNA and chemotaxonomic data supported the affiliation of strain BS11ᵀ to the genus Sphingosinicella. Strain BS11ᵀ could be differentiated genotypically and phenotypically from the recognized species of the genus Sphingosinicella. The novel isolate therefore represents a novel species, for which the name Sphingosinicella ginsenosidimutans sp. nov. is proposed, with the type strain BS11ᵀ (=KACC 16619T =JCM 18201ᵀ).
    Keywords menaquinones ; sodium chloride ; new species ; fatty acids ; agar ; ribosomal RNA ; Panax ; chemotaxonomy ; Sphingomonadaceae ; beta-glucosidase ; sequence homology ; nucleotide sequences ; DNA ; composts ; pH ; microbiology
    Language English
    Document type Article
    ISSN 1225-8873
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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