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  1. Article ; Online: Comparison of reminder-preference test to difference and preference tests: An affective approach yielding d-prime results for sensory difference and preference.

    Kim, Min-A / Kim, Myung-Shin / Lee, Ye-Jin / Lee, Hye-Seong

    Food research international (Ottawa, Ont.)

    2023  Volume 173, Issue Pt 2, Page(s) 113441

    Abstract: Preserving the sensory characteristics of reformulated products is crucial for a successful market launch. This underscores the need for precise measurement of consumer perceptions regarding sensory differences and preferences. One approach to assess ... ...

    Abstract Preserving the sensory characteristics of reformulated products is crucial for a successful market launch. This underscores the need for precise measurement of consumer perceptions regarding sensory differences and preferences. One approach to assess these aspects is through the utilization of the reminder-preference test, an affective difference-preference test employing the A-Not A with the reminder (A-Not AR) test design. This test measures sensory differences and preferences by employing a consolidated d' index rooted in signal detection theory and Thurstonian modeling. The aim of this study was to assess the efficacy of the reminder-preference test in measuring the degree of sensory differences and interpreting their relevance concerning consumer preference. To achieve this, the test was compared with both the A-Not AR difference test and the 2-alternative choice (2-AC) preference test. A lemon-lime-flavored carbonated drink and two types of reformulated samples were tested. Young female adult subjects were randomly divided into three groups. Each took one of the following three tests: the reminder-preference test, the reminder test of A-Not AR, or the 2-AC preference test. The reference framing in the reminder-preference and reminder tests was preceded by subjects watching the product's advertisement to assist them in framing evaluative criteria. This would also facilitate memorizing the perception of the reference product. Test sensitivity in reminder-preference matched reminder and 2-AC tests for sensory differences and preferences. Affective framing enhanced sensitivity to sensory differences resulting in a higher d' value of the sensory difference than the preference. From the results, it was possible to interpret that the measured sensory differences did not affect consumer preference. The test also illuminated brand marketing effects on preferences through branded reference comparison. Thus, the reminder-preference test shows potential as an efficient sensory method to measure sensory differences and the preference of consumers, while concurrently measuring the effects of marketing on the consumers. Therefore, it can assist in making efficient business decisions regarding product reformulations.
    MeSH term(s) Adult ; Humans ; Female ; Taste ; Decision Making ; Consumer Behavior ; Carbonated Beverages ; Commerce
    Language English
    Publishing date 2023-09-09
    Publishing country Canada
    Document type Randomized Controlled Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2023.113441
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: S100 Calcium-Binding Protein A9, a Potential Novel Diagnostic Biomarker for Idiopathic Pulmonary Fibrosis.

    Lee, Jong-Uk / Kim, Min Kyung / Kim, Myung-Shin / Lee, Sun Ju / Park, Seung-Lee / Chang, Hun Soo / Park, Jong-Sook / Park, Choon-Sik

    Journal of Korean medical science

    2024  Volume 39, Issue 1, Page(s) e13

    Abstract: Background: Neutrophilic inflammation is a characteristic feature of idiopathic pulmonary fibrosis (IPF). S100 calcium-binding protein A9 (S100A9) is a neutrophil-derived protein involved in the development of neutrophil-related chronic inflammatory ... ...

    Abstract Background: Neutrophilic inflammation is a characteristic feature of idiopathic pulmonary fibrosis (IPF). S100 calcium-binding protein A9 (S100A9) is a neutrophil-derived protein involved in the development of neutrophil-related chronic inflammatory disorders. However, the role of S100A9 in IPF remains unclear.
    Methods: We used enzyme-linked immunosorbent assays to measure S100A9 levels in bronchoalveolar lavage fluid (BALF) and serum obtained from healthy controls (HCs) and patients with IPF, non-specific interstitial pneumonia, hypersensitivity pneumonitis, and sarcoidosis.
    Results: Compared with HCs, BALF S100A9 levels were significantly higher in IPF patients (
    Conclusion: S100A9 is involved in the development and progression of IPF. Moreover, S100A9 levels in BALF and serum may be surrogate markers for IPF diagnosis and survival prediction, particularly when analyzed in combination with neutrophil percentages.
    MeSH term(s) Humans ; Idiopathic Pulmonary Fibrosis/diagnosis ; Inflammation ; Alveolitis, Extrinsic Allergic ; Bronchoalveolar Lavage Fluid ; Calgranulin B
    Chemical Substances Calgranulin B
    Language English
    Publishing date 2024-01-08
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 639262-3
    ISSN 1598-6357 ; 1011-8934
    ISSN (online) 1598-6357
    ISSN 1011-8934
    DOI 10.3346/jkms.2024.39.e13
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Reminder-preference test, affective difference-preference test using reference framing with a brand: 1. Sensitivity comparisons with the same-different difference-preference test.

    Kim, Min-A / Lee, Ye-Jin / Kim, Myung-Shin / Lee, Hye-Seong

    Food research international (Ottawa, Ont.)

    2022  Volume 155, Page(s) 111065

    Abstract: Attempts to reduce the sugar content of carbonated drinks are ongoing. Reducing the sugar content while maintaining the sensory quality requires the development of an effective sensory test method that can measure a consumers' perceived sensory ... ...

    Abstract Attempts to reduce the sugar content of carbonated drinks are ongoing. Reducing the sugar content while maintaining the sensory quality requires the development of an effective sensory test method that can measure a consumers' perceived sensory difference and preference of reformulated products against the reference product. The A-Not A with reminder (A-Not AR) method (sometimes called the reminder method) allows blind sensory tests of multiple test samples vs. a reference, while for the reference, marketing information, such as brand name and advertisements emphasizing the consumer benefit, can be provided to improve the marketing effects and consumer expectation. Thus, a preference test method can also simulate branded tests using A-Not AR design with a branded reference, making them suitable for consumer sensory tests. In the present study, an affective difference-preference test method using an A-Not AR design with the reference familiarization procedure of providing marketing information was developed, called the "reminder-preference test". The "reminder-preference test" sensitivity was compared with the previously suggested version of the "same-different difference-preference test", using signal detection d' analyses. A lemon-lime flavored carbonated drink was used as the reference and compared with two types of reformulations. The consumers were divided randomly into two groups that performed one of the two difference-preference test methods. With the "reminder-preference test", the consumers watched a commercial advertisement for the reference by providing its brand name before the tests to evoke the marketing effects. The proposed sensory test of the "reminder-preference test" was more sensitive than the "same-different difference-preference test", in terms of both sensory and preference discriminations. Hence, brand effects can be incorporated into the consumer sensory evaluation to achieve the reformulation objective and the potential of the "reminder-preference test" and its signal detection d' analyses to improve the effectiveness and efficiency of consumer sensory measurements.
    MeSH term(s) Carbohydrates ; Carbonated Beverages/analysis ; Consumer Behavior ; Marketing ; Sugars
    Chemical Substances Carbohydrates ; Sugars
    Language English
    Publishing date 2022-02-24
    Publishing country Canada
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2022.111065
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The First Korean Child of Jalili Syndrome with a Novel Missense Mutation in Cation Transport Mediator 4 (CNNM4): A Case Report.

    Lee, Ji Hye / Park, Shin Hae / Yim, Ji Sook / Kim, Myung Shin / Kim, Sin-Young

    Korean journal of ophthalmology : KJO

    2023  Volume 37, Issue 2, Page(s) 195–197

    MeSH term(s) Humans ; Child ; Mutation, Missense ; Cone-Rod Dystrophies/genetics ; Cations ; Republic of Korea ; Mutation ; Cation Transport Proteins/genetics
    Chemical Substances Cations ; CNNM4 protein, human ; Cation Transport Proteins
    Language English
    Publishing date 2023-03-23
    Publishing country Korea (South)
    Document type Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639346-9
    ISSN 2092-9382 ; 1011-8942
    ISSN (online) 2092-9382
    ISSN 1011-8942
    DOI 10.3341/kjo.2022.0144
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Protein stability governed by α1-2 helices in Pvr4 is essential for localization and cell death.

    Lee, Hye-Young / Choi, Jeen / Kang, Minji / Lee, Joo Hyun / Kim, Myung-Shin / Choi, Doil

    The Plant journal : for cell and molecular biology

    2023  Volume 116, Issue 2, Page(s) 510–523

    Abstract: Plant nucleotide-binding domain leucine-rich-repeat receptor (NLR) confers disease resistance to various pathogens by recognizing effectors derived from the pathogen. Previous studies have shown that overexpression of the CC domain in several NLRs ... ...

    Abstract Plant nucleotide-binding domain leucine-rich-repeat receptor (NLR) confers disease resistance to various pathogens by recognizing effectors derived from the pathogen. Previous studies have shown that overexpression of the CC domain in several NLRs triggers cell death, implying that the CC domain plays an important role as a signaling module. However, how CC domain transduces immune signals remains largely unknown. A Potyvirus-resistant NLR protein, Pvr4, possesses a CC domain (CC
    Language English
    Publishing date 2023-07-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.16388
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Reminder–preference test, affective difference-preference test using reference framing with a brand: 1. Sensitivity comparisons with the same–different difference–preference test

    Kim, Min-A / Lee, Ye-Jin / Kim, Myung-Shin / Lee, Hye-Seong

    Food research international. 2022 May, v. 155

    2022  

    Abstract: Attempts to reduce the sugar content of carbonated drinks are ongoing. Reducing the sugar content while maintaining the sensory quality requires the development of an effective sensory test method that can measure a consumers’ perceived sensory ... ...

    Abstract Attempts to reduce the sugar content of carbonated drinks are ongoing. Reducing the sugar content while maintaining the sensory quality requires the development of an effective sensory test method that can measure a consumers’ perceived sensory difference and preference of reformulated products against the reference product. The A-Not A with reminder (A-Not AR) method (sometimes called the reminder method) allows blind sensory tests of multiple test samples vs. a reference, while for the reference, marketing information, such as brand name and advertisements emphasizing the consumer benefit, can be provided to improve the marketing effects and consumer expectation. Thus, a preference test method can also simulate branded tests using A-Not AR design with a branded reference, making them suitable for consumer sensory tests. In the present study, an affective difference-preference test method using an A-Not AR design with the reference familiarization procedure of providing marketing information was developed, called the “reminder–preference test”. The “reminder–preference test” sensitivity was compared with the previously suggested version of the “same–different difference–preference test”, using signal detection d′ analyses. A lemon-lime flavored carbonated drink was used as the reference and compared with two types of reformulations. The consumers were divided randomly into two groups that performed one of the two difference-preference test methods. With the “reminder–preference test”, the consumers watched a commercial advertisement for the reference by providing its brand name before the tests to evoke the marketing effects. The proposed sensory test of the “reminder–preference test” was more sensitive than the “same–different difference–preference test”, in terms of both sensory and preference discriminations. Hence, brand effects can be incorporated into the consumer sensory evaluation to achieve the reformulation objective and the potential of the “reminder–preference test” and its signal detection d′ analyses to improve the effectiveness and efficiency of consumer sensory measurements.
    Keywords carbonated beverages ; consumer attitudes ; food research ; sensory evaluation ; sensory properties ; sugar content
    Language English
    Dates of publication 2022-05
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2022.111065
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Single-cell transcriptome sequencing of plant leaf expressing anti-HER2 VHH-FcK cancer therapeutic protein.

    Kim, Myung-Shin / Lee, Seung-Won / Kim, Kibum / Kim, Yerin / Hwang, Hyunjoo / Hinterdorfer, Peter / Choi, Doil / Ko, Kisung

    Scientific data

    2023  Volume 10, Issue 1, Page(s) 911

    Abstract: The transgenic plant is a promising strategy for the production of highly valuable biotherapeutic proteins such as recombinant vaccines and antibodies. To achieve an efficient level of protein production, codon sequences and expression cassette elements ... ...

    Abstract The transgenic plant is a promising strategy for the production of highly valuable biotherapeutic proteins such as recombinant vaccines and antibodies. To achieve an efficient level of protein production, codon sequences and expression cassette elements need to be optimized. However, the systematical expression of recombinant proteins in plant biomass can generally be controlled for the production of therapeutic proteins after the generation of transgenic plants. Without understanding the transgene expression patterns in plant tissue, it is difficult to enhance further production levels. In this study, single-cell RNA-sequencing (scRNA-seq) analysis of transgenic tobacco (Nicotiana tabacum) leaf, expressing an immunotherapeutic llama antibody against breast cancer, anti-HER2 VHH-Fc, was conducted to obtain data on the expression pattern of tissue-specific cells. These high-quality scRNA-seq data enabled the identification of gene expression patterns by cell types, which can be applied to select the best cell types or tissues for the high production of these recombinant antibodies. These data provide a foundation to elucidate the mechanisms that regulate the biosynthesis of recombinant proteins in N. tabacum.
    MeSH term(s) Female ; Humans ; Breast Neoplasms/metabolism ; Plant Leaves/genetics ; Plants, Genetically Modified/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Transcriptome
    Chemical Substances Recombinant Proteins
    Language English
    Publishing date 2023-12-19
    Publishing country England
    Document type Dataset ; Journal Article
    ZDB-ID 2775191-0
    ISSN 2052-4463 ; 2052-4463
    ISSN (online) 2052-4463
    ISSN 2052-4463
    DOI 10.1038/s41597-023-02833-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The Phytophthora nucleolar effector Pi23226 targets host ribosome biogenesis to induce necrotrophic cell death.

    Lee, Soeui / Kim, Jaehwan / Kim, Myung-Shin / Min, Cheol Woo / Kim, Sun Tae / Choi, Sang-Bong / Lee, Joo Hyun / Choi, Doil

    Plant communications

    2023  Volume 4, Issue 5, Page(s) 100606

    Abstract: Pathogen effectors target diverse subcellular organelles to manipulate the plant immune system. Although the nucleolus has emerged as a stress marker and several effectors are localized in the nucleolus, the roles of nucleolar-targeted effectors remain ... ...

    Abstract Pathogen effectors target diverse subcellular organelles to manipulate the plant immune system. Although the nucleolus has emerged as a stress marker and several effectors are localized in the nucleolus, the roles of nucleolar-targeted effectors remain elusive. In this study, we showed that Phytophthora infestans infection of Nicotiana benthamiana results in nucleolar inflation during the transition from the biotrophic to the necrotrophic phase. Multiple P. infestans effectors were localized in the nucleolus: Pi23226 induced cell death in N. benthamiana and nucleolar inflation similar to that observed in the necrotrophic stage of infection, whereas its homolog Pi23015 and a deletion mutant (Pi23226ΔC) did not induce cell death or affect nucleolar size. RNA immunoprecipitation and individual-nucleotide-resolution UV crosslinking and immunoprecipitation sequencing analysis indicated that Pi23226 bound to the 3' end of 25S rRNA precursors, resulting in accumulation of unprocessed 27S pre-rRNAs. The nucleolar stress marker NAC082 was strongly upregulated under Pi23226-expressing conditions. Pi23226 subsequently inhibited global protein translation in host cells by interacting with ribosomes. Pi23226 enhanced P. infestans pathogenicity, indicating that Pi23226-induced ribosome malfunction and cell death were beneficial for pathogenesis in the host. Our results provide evidence for the molecular mechanism underlying RNA-binding effector activity in host ribosome biogenesis and lead to new insights into the nucleolar action of effectors in pathogenesis.
    MeSH term(s) Cell Nucleolus/genetics ; Cell Nucleolus/metabolism ; Cell Death ; Ribosomes ; Nicotiana/genetics ; Nicotiana/metabolism ; Phytophthora infestans
    Language English
    Publishing date 2023-04-23
    Publishing country China
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2590-3462
    ISSN (online) 2590-3462
    DOI 10.1016/j.xplc.2023.100606
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Prognostic value of European LeukemiaNet 2022 criteria and genomic clusters using machine learning in older adults with acute myeloid leukemia.

    Park, Silvia / Kim, Tong Yoon / Cho, Byung-Sik / Kwag, Daehun / Lee, Jong-Mi / Kim, MyungShin / Kim, Yonggoo / Koo, Jamin / Raman, Anjali / Kim, Tae Kon / Kim, Hee-Je

    Haematologica

    2024  Volume 109, Issue 4, Page(s) 1095–1106

    Abstract: This study aimed to validate the new European Leukemia Net (ELN) 2022 criteria for genetic risk stratification in older adults with acute myeloid leukemia (AML) and to determine the most likely set of clusters of similar cytogenetic and mutation ... ...

    Abstract This study aimed to validate the new European Leukemia Net (ELN) 2022 criteria for genetic risk stratification in older adults with acute myeloid leukemia (AML) and to determine the most likely set of clusters of similar cytogenetic and mutation properties correlated with survival outcomes in three treatment groups: intensive chemotherapy (IC), hypomethylating agents (HMA) alone, and HMA plus venetoclax (HMA/VEN). The study included 279 patients (aged ≥60 years) who received IC (N=131), HMA (N=76), and HMA/VEN (N=72) between July 2017 and October 2021. No significant differences were observed in survival among the groups according to ELN 2022 risk stratification. Unsupervised hierarchical clustering analysis identified nine genomic clusters (C1-9) with varying survival outcomes depending on treatment type. For example, C4 (predominant for core binding factor-AML) displayed a favorable prognosis in the IC group, but not in the HMA or HMA/VEN groups. The HMA/VEN group had better outcomes than the HMA group in many clusters (C1, 2, 3, and 5); however, the addition of VEN to HMA or IC did not improve the survival outcomes compared with those of HMA alone in C7 and C9 (predominant for -5, del(5q), -7, -17/abn(17p), complex karyotypes, and mutated TP53). The study highlights the limitations of ELN genetic risk stratification in older adults with AML. It emphasizes the need for a more comprehensive approach that considers co-occurring somatic mutations to guide treatment selection in older adults with AML.
    MeSH term(s) Humans ; Aged ; Prognosis ; Leukemia, Myeloid, Acute/diagnosis ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/drug therapy ; Risk Factors ; Genomics ; Machine Learning ; Retrospective Studies ; Bridged Bicyclo Compounds, Heterocyclic ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use
    Chemical Substances Bridged Bicyclo Compounds, Heterocyclic
    Language English
    Publishing date 2024-04-01
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 2333-4
    ISSN 1592-8721 ; 0017-6567 ; 0390-6078
    ISSN (online) 1592-8721
    ISSN 0017-6567 ; 0390-6078
    DOI 10.3324/haematol.2023.283606
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Genome Resequencing, Improvement of Variant Calling, and Population Genomic Analyses Provide Insights into the Seedlessness in the Genus

    Kim, Myung-Shin / Hur, Youn Young / Kim, Ji Hong / Jeong, Soon-Chun

    G3 (Bethesda, Md.)

    2020  Volume 10, Issue 9, Page(s) 3365–3377

    Abstract: The seedlessness of grape derived from stenospermocarpy is one of the most prized traits of table or raisin grapes. It is controlled by a complex genetic system containing one dominant gene and multiple recessive genes. Here, we collected dense variation ...

    Abstract The seedlessness of grape derived from stenospermocarpy is one of the most prized traits of table or raisin grapes. It is controlled by a complex genetic system containing one dominant gene and multiple recessive genes. Here, we collected dense variation data from high-depth resequencing data of seeded, seedless, and wild relative grape genomes sequenced to > 37x mean depth. Variant calls were made using a modified variant calling pipeline that was suitable for highly diverse interspecific grape accessions. The modified pipeline enabled us to call several million more variants than the commonly recommended pipeline. The quality was validated by Sanger sequencing data and subsequently supported by the genetic population structure and the phylogenetic tree constructed using the obtained variation data, results of which were generally consistent with known pedigree and taxonomic classifications. Variation data enabled us to confirm a dominant gene and identify recessive loci for seedlessness. Incidentally, we found that grape cultivar Rizamat contains an ancestral chromosomal region of the dominant gene in Sultanina, a predominant seedlessness donor cultivar. Furthermore, we predicted new candidate causal genes including
    MeSH term(s) Genomics ; Metagenomics ; Phylogeny ; Seeds/genetics ; Vitis/genetics
    Language English
    Publishing date 2020-09-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1534/g3.120.401521
    Database MEDical Literature Analysis and Retrieval System OnLINE

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