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Article ; Online: Induction of TNF-α by Filifactor alocis in THP-1 macrophagic cells.

Kim, So-Hee / Kang, In-Chol

2023 Volume 155, Page(s) 105806

Abstract: Objectives: Filifactor alocis is an emerging periodontal pathogen, and macrophage-produced tumor necrosis factor-α (TNF-α) plays important roles in periodontal pathogenesis. In this study, we investigated F. alocis-stimulated TNF-α production in THP-1 ... ...

Abstract	Objectives: Filifactor alocis is an emerging periodontal pathogen, and macrophage-produced tumor necrosis factor-α (TNF-α) plays important roles in periodontal pathogenesis. In this study, we investigated F. alocis-stimulated TNF-α production in THP-1 macrophagic cells. Design: Phorbol 12-myristate 13-acetate-differentiated THP-1 macrophagic cells were challenged with F. alocis ATCC 35896 for various durations. TNF-α mRNA expression and protein secretion were determined using RT-PCR and ELISA, respectively. Activation of protein kinases and transcription factor proteins was evaluated by Western blot analysis. Results: Live F. alocis stimulated THP-1 cells to produce TNF-α in a dose-dependent manner. However, glutaraldehyde-killed or heat-killed F. alocis showed no effectiveness for TNF-α induction. In contrast, both live and killed Porphyromonas gingivalis robustly increased TNF-α expression. Furthermore, F. alocis was unable to stimulate TNF-α expression in Toll-like receptor 2 (TLR2) knockout THP-1 cells. F. alocis activated all three mitogen-activated protein kinases: extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK). Pharmacological inhibition of ERK and JNK, but not p38, significantly reduced F. alocis-induced TNF-α production. Finally, increased levels of phospho-c-Jun were detected in F. alocis-stimulated THP-1 cells. Conclusions: These results suggest that F. alocis induces TNF-α production in THP-1 macrophagic cells primarily by activating the TLR2, JNK, and c-Jun pathways.
Language	English
Publishing date	2023-09-16
Publishing country	England
Document type	Journal Article
ZDB-ID	80227-x
ISSN	1879-1506 ; 0003-9969
ISSN (online)	1879-1506
ISSN	0003-9969
DOI	10.1016/j.archoralbio.2023.105806
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Article ; Online: KoCVAM-led development of phototoxicity alternative test method using reconstructed human epidermis model (KeraSkin™).

Kang, Nam-Hee / Kim, So-Hee / Kim, Joohwan

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2024 Volume 188, Page(s) 114698

Abstract: Phototoxicity is an acute toxic reaction induced by topical skin exposure to photoreactive chemicals followed by exposure to environmental light and thus chemicals that absorb UV are recommended to be evaluated for phototoxic potential. There are ... ...

Abstract	Phototoxicity is an acute toxic reaction induced by topical skin exposure to photoreactive chemicals followed by exposure to environmental light and thus chemicals that absorb UV are recommended to be evaluated for phototoxic potential. There are currently three internationally harmonized alternative test methods for phototoxicity. One of them is the in vitro Phototoxicity: RhE Phototoxicity test method (OECD TG498). Korean center for the Validation of Alternative Methods (KoCVAM) developed an in vitro phototoxicity test method using a KeraSkin™ reconstructed human epidermis model (KeraSkin™ Phototoxicity Assay) as a 'me-too' test method of OECD TG498. For the development and optimization of KeraSkin™ Phototoxicity Assay, the following test chemicals were used: 6 proficiency chemicals in OECD TG498 (3 phototoxic and 3 non-phototoxic), 6 reference chemicals in OECD Performance Standard No. 356 (excluding the proficiency test chemicals, 3 phototoxic and 3 non-phototoxic) and 13 additional chemicals (7 phototoxic and 6 non-phototoxic). Based on the test results generated from the test chemicals above, the overall predictive capacity of KeraSkin™ Phototoxicity Assay was calculated. In particular, the assay exhibited 100 % accuracy, 100 % sensitivity, and 100 % specificity. Therefore, it fulfills the requirements to be included as a 'me-too' test method in OECD TG498.
MeSH term(s)	Humans ; Epidermis/drug effects ; Epidermis/radiation effects ; Dermatitis, Phototoxic ; Animal Testing Alternatives/methods ; Ultraviolet Rays ; Toxicity Tests/methods ; Models, Biological
Language	English
Publishing date	2024-04-26
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2024.114698
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Article ; Online: Ovarian tumor deubiquitinase 6A regulates cell proliferation via deubiquitination of nucleolin and caspase‑7.

Kim, So-Hee / Baek, Kwang-Hyun

International journal of oncology

2022 Volume 61, Issue 4

Abstract: Most proteins maintain protein homeostasis via post‑translational modifications, including the ubiquitin‑proteasome system. Deubiquitinating enzymes (DUBs) have essential intercellular roles, such as responses to DNA damage, proteolysis and apoptosis. ... ...

Abstract	Most proteins maintain protein homeostasis via post‑translational modifications, including the ubiquitin‑proteasome system. Deubiquitinating enzymes (DUBs) have essential intercellular roles, such as responses to DNA damage, proteolysis and apoptosis. Therefore, it is important to understand DUB‑related diseases to identify DUBs that target abnormally regulated proteins in cells. Ovarian tumor deubiquitinase 6A (OTUD6A) was previously reported as a downregulated DUB in HCT116 cells with p53 knockdown. Therefore, it was expected that the relationship between OTUD6A and p53 would affect cell proliferation. In the present study, putative substrates of OTUD6A related to the p53 signaling pathway were identified. Application of liquid chromatography‑tandem mass spectrometry and proteomic analysis led to the identification of nucleolin (known to bind p53) as a binding protein. In addition, immunoprecipitation studies determined that caspase‑7, an apoptotic protein, is associated with p53 signaling and is regulated by OTUD6A. It was further identified that OTUD6A regulates the protein stability of nucleolin, but not caspase‑7. It was also demonstrated that OTUD6A acts as a respective DUB through the deubiquitination of K48‑linked polyubiquitin chain of nucleolin and the K63‑linked polyubiquitin chain of caspase‑7. Furthermore, overexpression of OTUD6A induced cell proliferation via enhancing cell cycle progression of MCF7 cells. Taken together, OTUD6A may be proposed as a target for anticancer therapy.
MeSH term(s)	Caspase 7/metabolism ; Cell Proliferation ; Deubiquitinating Enzymes/genetics ; Deubiquitinating Enzymes/metabolism ; Female ; Humans ; Ovarian Neoplasms/genetics ; Phosphoproteins/metabolism ; Polyubiquitin/genetics ; Polyubiquitin/metabolism ; Proteomics ; RNA-Binding Proteins/metabolism ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism ; Ubiquitination ; Nucleolin
Chemical Substances	Phosphoproteins ; RNA-Binding Proteins ; Tumor Suppressor Protein p53 ; Polyubiquitin (120904-94-1) ; Deubiquitinating Enzymes (EC 3.4.19.12) ; OTUD6A protein, human (EC 3.4.19.12) ; CASP7 protein, human (EC 3.4.22.-) ; Caspase 7 (EC 3.4.22.-)
Language	English
Publishing date	2022-09-09
Publishing country	Greece
Document type	Journal Article
ZDB-ID	1154403-x
ISSN	1791-2423 ; 1019-6439
ISSN (online)	1791-2423
ISSN	1019-6439
DOI	10.3892/ijo.2022.5417
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Article ; Online: Regulation of Cancer Metabolism by Deubiquitinating Enzymes: The Warburg Effect.

Kim, So-Hee / Baek, Kwang-Hyun

International journal of molecular sciences

2021 Volume 22, Issue 12

Abstract: Cancer is a disorder of cell growth and proliferation, characterized by different metabolic pathways within normal cells. The Warburg effect is a major metabolic process in cancer cells that affects the cellular responses, such as proliferation and ... ...

Abstract	Cancer is a disorder of cell growth and proliferation, characterized by different metabolic pathways within normal cells. The Warburg effect is a major metabolic process in cancer cells that affects the cellular responses, such as proliferation and apoptosis. Various signaling factors down/upregulate factors of the glycolysis pathway in cancer cells, and these signaling factors are ubiquitinated/deubiquitinated via the ubiquitin-proteasome system (UPS). Depending on the target protein, DUBs act as both an oncoprotein and a tumor suppressor. Since the degradation of tumor suppressors and stabilization of oncoproteins by either negative regulation by E3 ligases or positive regulation of DUBs, respectively, promote tumorigenesis, it is necessary to suppress these DUBs by applying appropriate inhibitors or small molecules. Therefore, we propose that the DUBs and their inhibitors related to the Warburg effect are potential anticancer targets.
MeSH term(s)	Animals ; Apoptosis ; Deubiquitinating Enzymes/metabolism ; Humans ; Neoplasms/metabolism ; Neoplasms/pathology ; Ubiquitination ; Warburg Effect, Oncologic
Chemical Substances	Deubiquitinating Enzymes (EC 3.4.19.12)
Language	English
Publishing date	2021-06-08
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22126173
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Article ; Online: Interconnected channels through polypropylene and cellulose acetate by utilizing lactic acid for stable separators.

Kim, So Hee / Kang, Sang Wook

Chemical communications (Cambridge, England)

2021 Volume 57, Issue 71, Page(s) 8965–8968

Abstract: In this study, an eco-friendly and inexpensive cellulose acetate (CA) separator was fabricated and a method of making a single film by combining a polypropylene (PP) film and cellulose was proposed. The CA solution was coated on the PP film with a doctor ...

Abstract	In this study, an eco-friendly and inexpensive cellulose acetate (CA) separator was fabricated and a method of making a single film by combining a polypropylene (PP) film and cellulose was proposed. The CA solution was coated on the PP film with a doctor blade and water treatment was applied to the bonded polymer to create interconnected pores and completely bond the CA onto the PP. In addition, lactic acid was added to CA to induce a plasticizing effect for abundant pore formation. The binding was confirmed using FT-IR and SEM, and the pore size generated from the CA side was found to be less than 1 μm on average. TGA was used to measure the thermal stability of the connected polymers.
MeSH term(s)	Cellulose/analogs & derivatives ; Cellulose/chemistry ; Lactic Acid/chemistry ; Membranes, Artificial ; Polypropylenes/chemistry ; Porosity
Chemical Substances	Membranes, Artificial ; Polypropylenes ; Lactic Acid (33X04XA5AT) ; acetylcellulose (3J2P07GVB6) ; Cellulose (9004-34-6)
Language	English
Publishing date	2021-09-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/d1cc02955j
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Article: Inhibitory effects of non-thermal atmospheric plasma on

Kim, So Hee / Park, Sung-Hee / Min, Sung Gi / Park, Shin Young

Heliyon

2023 Volume 9, Issue 9, Page(s) e19575

Abstract: Food-borne bacteria have frequently been detected ... ...

Abstract	Food-borne bacteria have frequently been detected in
Language	English
Publishing date	2023-09-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e19575
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Article ; Online: Diverse methods of reducing and confirming false-positive results of loop-mediated isothermal amplification assays: A review.

Kim, So-Hee / Lee, So-Young / Kim, Unji / Oh, Se-Wook

Analytica chimica acta

2023 Volume 1280, Page(s) 341693

Abstract: Loop-mediated isothermal amplification (LAMP), a rapid and sensitive isothermal nucleic acid amplification method, is a promising alternative to other molecular amplification techniques due to its superior specificity and sensitivity. However, due to ... ...

Abstract	Loop-mediated isothermal amplification (LAMP), a rapid and sensitive isothermal nucleic acid amplification method, is a promising alternative to other molecular amplification techniques due to its superior specificity and sensitivity. However, due to primer dimerization, LAMP results in nonspecific and nontemplate amplification. And during the amplification confirmation process, there is carry-over contamination. These factors can result in false-positive results that overestimate the amount of DNA, preventing accurate detection. This review outlined several techniques for reducing false-positive LAMP results before amplification and confirming false-positive results after amplification. Before the amplification step, DNA polymerase activity can be decreased with organic additives such as dimethyl sulfoxide, betaine, and pullulan to prevent nonspecific amplification. The enzyme uracil-DNA-glycosylase (UDG) can eliminate false-positive results caused by carry-over contamination, and the hot-start effect with gold nanoparticles can reduce nonspecific amplification. When confirming false-positive results using clustered regularly interspaced short palindromic repeats, guide RNA accurately detects LAMP amplification, allowing differentiation from nonspecific amplification. By confirming amplification, the colorimetric change in the deoxyribozyme (DNAzyme) formed by the reaction of the G-quadruplex sequence of the LAMP amplicon and hemin can distinguish false-positive results. Lateral flow immunoassay can distinguish false-positive results by accurately recognizing hybridized probes to LAMP amplicons.
MeSH term(s)	Gold ; Metal Nanoparticles ; Nucleic Acid Amplification Techniques/methods ; DNA/genetics ; Sensitivity and Specificity
Chemical Substances	Gold (7440-57-5) ; DNA (9007-49-2)
Language	English
Publishing date	2023-08-07
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	1483436-4
ISSN	1873-4324 ; 0003-2670
ISSN (online)	1873-4324
ISSN	0003-2670
DOI	10.1016/j.aca.2023.341693
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Article: Interconnected channels through polypropylene and cellulose acetate by utilizing lactic acid for stable separators

Kim, So Hee / Kang, Sang Wook

Chemical communications. 2021 Sept. 6, v. 57, no. 71

2021

Abstract	In this study, an eco-friendly and inexpensive cellulose acetate (CA) separator was fabricated and a method of making a single film by combining a polypropylene (PP) film and cellulose was proposed. The CA solution was coated on the PP film with a doctor blade and water treatment was applied to the bonded polymer to create interconnected pores and completely bond the CA onto the PP. In addition, lactic acid was added to CA to induce a plasticizing effect for abundant pore formation. The binding was confirmed using FT-IR and SEM, and the pore size generated from the CA side was found to be less than 1 μm on average. TGA was used to measure the thermal stability of the connected polymers.
Keywords	cellulose ; cellulose acetate ; lactic acid ; polypropylenes ; porosity ; thermal stability ; water treatment
Language	English
Dates of publication	2021-0906
Size	p. 8965-8968.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/d1cc02955j
Database	NAL-Catalogue (AGRICOLA)

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Article: Preparation of highly stable cellulose separator by incorporation of lactic acid

Kim, So Hee / Kang, Sang Wook

Cellulose. 2021 Oct., v. 28, no. 15

2021

Abstract: In this study, a porous membrane consisting of lactic acid and cellulose acetate was fabricated using a low-cost and pro-environmental process. In order to solve the thermostability of the membrane, cellulose acetate, which has higher melting point than ... ...

Abstract	In this study, a porous membrane consisting of lactic acid and cellulose acetate was fabricated using a low-cost and pro-environmental process. In order to solve the thermostability of the membrane, cellulose acetate, which has higher melting point than polypropylene (PP) and polyethylene (PE), was used as the polymer matrix. The wetting action of the lactic acid induced a plasticization effect in the cellulose acetate chain, forming several small pores during the hydrostatic treatment. The surface and cross-section of the membranes were observed using scanning electron microscope. The abundant pores were observed in the plasticized area on the surface. The interaction between cellulose acetate and lactic acid was investigated using Fourier transform infrared spectroscopy. In C=O and C–O peak, the peak shift was observed depending on whether or not the water pressure was treated, and the interaction of CA and lactic acid was confirmed. Furthermore, thermogravimetric analysis was performed to determine the thermal stability of the membrane and the removal of lactic acid by the hydrostatic treatment. As a result, thermal stability of CA polymer became weakened due to plasticization of lactic acid, but showed similar stability to neat CA after hydraulic treatment. In addition, the optimum composition and performance of the composite were investigated via water flux measurement. Furthermore, the average pore diameter and porosity of the cellulose acetate/lactic acid membrane measured using a porosimeter was 0.38 μm and 65.3%, respectively.
Keywords	Fourier transform infrared spectroscopy ; cellulose ; cellulose acetate ; lactic acid ; polyethylene ; polypropylenes ; porosity ; thermal stability ; thermogravimetry
Language	English
Dates of publication	2021-10
Size	p. 10055-10063.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	1496831-9
ISSN	1572-882X ; 0969-0239
ISSN (online)	1572-882X
ISSN	0969-0239
DOI	10.1007/s10570-021-04144-7
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Article: Role of AMPK in Regulation of Oxaliplatin-Resistant Human Colorectal Cancer.

Park, Sun Young / Chung, Ye Seo / Park, So Yeon / Kim, So Hee

Biomedicines

2022 Volume 10, Issue 11

Abstract: Oxaliplatin is a platinum analog that can interfere with DNA replication and transcription. Continuous exposure to oxaliplatin results in chemoresistance; however, this mechanism is not well known. In this study, oxaliplatin-resistant (OR) colorectal ... ...

Abstract	Oxaliplatin is a platinum analog that can interfere with DNA replication and transcription. Continuous exposure to oxaliplatin results in chemoresistance; however, this mechanism is not well known. In this study, oxaliplatin-resistant (OR) colorectal cancer (CRC) cells of HCT116, HT29, SW480 and SW620 were established by gradually increasing the drug concentration to 2.5 μM. The inhibitory concentrations of cell growth by 50% (IC
Language	English
Publishing date	2022-10-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10112690
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