Artikel ; Online: γ-Cyclodextrin-polyurethane copolymer adsorbent for selective removal of endotoxin from DNA solution.
2013 Band 443, Heft 1, Seite(n) 41–45
Abstract: Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that ... ...
| Abstract | Copolymer particles for removal of endotoxins (lipopolysaccharides, LPSs) were prepared by suspension copolymerization of γ-cyclodextrin (CyD) and 1,6-hexamethylenediisocyanate. The LPS-removing activity of the copolymer particles was compared with that of poly(ε-lysine)-immobilized Cellufine (cationic adsorbent) or polystyrene particles (hydrophobic adsorbent) by a batch method. When DNA was present in solution with LPSs under physiological conditions (pH 6.0, ionic strength of μ = 0.05-0.8), LPS-removing activity of the cationic or hydrophobic adsorbent was unsatisfactory because both the DNA and the LPSs were adsorbed onto each adsorbent. By contrast, the copolymer particles with γ-CyD cavity (CyD content: 14-20 mol%) could selectively remove LPSs from a DNA solution (50 μg ml(-1), pH 6.0, and μ = 0.05-0.2) containing LPSs (15 EU ml(-1)) without the adsorption of DNA. The residual concentration of LPSs in the treated DNA solution was below 0.1 EU ml(-1), and the recovery of DNA was 99%. |
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| Mesh-Begriff(e) | Adsorption ; Animals ; Cyanates/chemistry ; DNA/isolation & purification ; Escherichia coli/chemistry ; Hydrogen-Ion Concentration ; Isocyanates ; Lipopolysaccharides/isolation & purification ; Male ; Microspheres ; Osmolar Concentration ; Polymerization ; Polyurethanes/chemistry ; Salmon ; Sensitivity and Specificity ; Solid Phase Extraction ; Solutions ; Spectroscopy, Fourier Transform Infrared ; Spermatozoa/chemistry ; gamma-Cyclodextrins/chemistry |
| Chemische Substanzen | Cyanates ; Isocyanates ; Lipopolysaccharides ; Polyurethanes ; Solutions ; gamma-Cyclodextrins ; 1,6-hexamethylene diisocyanate (0I70A3I1UF) ; DNA (9007-49-2) ; gamma-cyclodextrin (KZJ0BYZ5VA) |
| Sprache | Englisch |
| Erscheinungsdatum | 2013-12-01 |
| Erscheinungsland | United States |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1110-1 |
| ISSN | 1096-0309 ; 0003-2697 |
| ISSN (online) | 1096-0309 |
| ISSN | 0003-2697 |
| DOI | 10.1016/j.ab.2013.08.010 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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