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Article ; Online: Dynamic regulation of LINC complex composition and function across tissues and contexts.

King, Megan C

2023 Volume 597, Issue 22, Page(s) 2823–2832

Abstract: The concept of mechanotransduction to the nucleus through a direct force transmission mechanism has fascinated cell biologists for decades. Central to such a mechanism is the linker of nucleoskeleton and cytoskeleton (LINC) complex, which spans the ... ...

Abstract	The concept of mechanotransduction to the nucleus through a direct force transmission mechanism has fascinated cell biologists for decades. Central to such a mechanism is the linker of nucleoskeleton and cytoskeleton (LINC) complex, which spans the nuclear envelope to couple the cytoplasmic cytoskeleton to the nuclear lamina. In reality, there is not one LINC complex identity, but instead, a family of protein configurations of varied composition that exert both shared and unique functions. Regulated expression of LINC complex components, splice variants, and mechanoresponsive protein turnover mechanisms together shape the complement of LINC complex forms present in a given cell type. Disrupting specific gene(s) encoding LINC complex components therefore gives rise to a range of organismal defects. Moreover, evidence suggests that the mechanical environment remodels LINC complexes, providing a feedback mechanism by which cellular context influences the integration of the nucleus into the cytoskeleton. In particular, evidence for crosstalk between the nuclear and cytoplasmic intermediate filament networks communicated through the LINC complex represents an emerging theme in this active area of ongoing investigation.
MeSH term(s)	Mechanotransduction, Cellular/physiology ; Cytoskeleton/metabolism ; Microtubules/metabolism ; Nuclear Matrix/metabolism ; Nuclear Envelope ; Cell Nucleus/metabolism
Language	English
Publishing date	2023-10-24
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1002/1873-3468.14757
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Article ; Online: It is time to reconsider the culture of volunteerism in undergraduate research.

King, Megan C

Journal of cell science

2023 Volume 136, Issue 24

Language	English
Publishing date	2023-12-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.261865
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Article ; Online: Dynamic regulation of LINC complex composition and function across tissues and contexts

King, Megan C.

FEBS Letters. 2023 Nov., v. 597, no. 22 p.2823-2832

2023

Abstract	The concept of mechanotransduction to the nucleus through a direct force transmission mechanism has fascinated cell biologists for decades. Central to such a mechanism is the linker of nucleoskeleton and cytoskeleton (LINC) complex, which spans the nuclear envelope to couple the cytoplasmic cytoskeleton to the nuclear lamina. In reality, there is not one LINC complex identity, but instead, a family of protein configurations of varied composition that exert both shared and unique functions. Regulated expression of LINC complex components, splice variants, and mechanoresponsive protein turnover mechanisms together shape the complement of LINC complex forms present in a given cell type. Disrupting specific gene(s) encoding LINC complex components therefore gives rise to a range of organismal defects. Moreover, evidence suggests that the mechanical environment remodels LINC complexes, providing a feedback mechanism by which cellular context influences the integration of the nucleus into the cytoskeleton. In particular, evidence for crosstalk between the nuclear and cytoplasmic intermediate filament networks communicated through the LINC complex represents an emerging theme in this active area of ongoing investigation.
Keywords	complement ; cytoskeleton ; genes ; mechanotransduction ; nuclear lamina ; nuclear membrane ; protein metabolism
Language	English
Dates of publication	2023-11
Size	p. 2823-2832.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	REVIEW
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1002/1873-3468.14757
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Article ; Online: Nuclear pore complexes feel the strain.

Lusk, C Patrick / King, Megan C

Molecular cell

2021 Volume 81, Issue 24, Page(s) 4962–4963

Abstract: A cryo-electron tomography structure of the human nuclear pore complex captured in cellulo by Schuller, Wojtynek et al. reveals that nuclear envelope tension expands the central transport channel and imposes asymmetry in the pore membrane. ...

Abstract	A cryo-electron tomography structure of the human nuclear pore complex captured in cellulo by Schuller, Wojtynek et al. reveals that nuclear envelope tension expands the central transport channel and imposes asymmetry in the pore membrane.
MeSH term(s)	Humans ; Nuclear Envelope ; Nuclear Pore ; Nuclear Pore Complex Proteins
Chemical Substances	Nuclear Pore Complex Proteins
Language	English
Publishing date	2021-12-16
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	1415236-8
ISSN	1097-4164 ; 1097-2765
ISSN (online)	1097-4164
ISSN	1097-2765
DOI	10.1016/j.molcel.2021.11.029
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Article ; Online: Elasticity of spheres with buckled surfaces.

Tian, Yingzhen / McCarthy, Megan / King, Megan / Mochrie, S G J

Physical review. E

2023 Volume 107, Issue 6-2, Page(s) 65003

Abstract: The buckling instabilities of core-shell systems, comprising an interior elastic sphere, attached to an exterior shell, have been proposed to underlie myriad biological morphologies. To fully discuss such systems, however, it is important to properly ... ...

Abstract	The buckling instabilities of core-shell systems, comprising an interior elastic sphere, attached to an exterior shell, have been proposed to underlie myriad biological morphologies. To fully discuss such systems, however, it is important to properly understand the elasticity of the spherical core. Here, by exploiting well-known properties of the solid harmonics, we present a simple, direct method for solving the linear elastic problem of spheres and spherical voids with surface deformations, described by a real spherical harmonic. We calculate the corresponding bulk elastic energies, providing closed-form expressions for any values of the spherical harmonic degree (l), Poisson ratio, and shear modulus. We find that the elastic energies are independent of the spherical harmonic index (m). Using these results, we revisit the buckling instability experienced by a core-shell system comprising an elastic sphere, attached within a membrane of fixed area, that occurs when the area of the membrane sufficiently exceeds the area of the unstrained sphere [C. Fogle et al., Phys. Rev. E 88, 052404 (2013)1539-375510.1103/PhysRevE.88.052404]. We determine the phase diagram of the core-shell sphere's shape, specifying what value of l is realized as a function of the area mismatch and the core-shell elasticity. We also determine the shape phase diagram for a spherical void bounded by a fixed-area membrane.
Language	English
Publishing date	2023-07-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2844562-4
ISSN	2470-0053 ; 2470-0045
ISSN (online)	2470-0053
ISSN	2470-0045
DOI	10.1103/PhysRevE.107.065003
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Article ; Online: Hidden cargo: The impact of historical shipping trade on the recent-past and contemporary non-native flora of northeastern United States.

Schmidt, Ryan J / King, Megan R / Aronson, Myla F J / Struwe, Lena

American journal of botany

2023 Volume 110, Issue 9, Page(s) e16224

Abstract: Premise: Understanding establishment and spread of non-native plants is important in the face of a homogenizing global flora. While many studies focus on successful, invasive species, fewer have studied failed plant introductions. Until the early 1900s, ...

Abstract	Premise: Understanding establishment and spread of non-native plants is important in the face of a homogenizing global flora. While many studies focus on successful, invasive species, fewer have studied failed plant introductions. Until the early 1900s, large quantities of ship ballast, often containing foreign plant propagules, were deposited in New Jersey (USA). The resulting ballast flora is documented in extensive herbarium records, providing us a unique opportunity to analyze successes and failures of novel plant species introductions. Methods: We used digitized specimens from 75 herbaria to study 264 non-native species introduced into New Jersey through 19th century ballast deposition. We used spatial (density-based clustering; HDBSCAN) and temporal analyses of species retention and geographic spread to quantify disappearance rate, survival, and dispersion through time and define trajectory groups. Results: Four distinct trajectory groups were identified: waif (only present during import; 32% of species), short-term (disappeared quickly; 20%), established-limited spread (survives locally, 30%), and established-widespread (widespread, 18%). Species disappearance rate was highest during ballast deposition and decreased soon after deposition stopped around 1900. Spatial patterns showed a strong association with 19th century railroads for inland dispersal from ports. The disappearance rate and spatial analyses are robust to herbarium collection bias. Conclusions: This study using New Jersey as a model is one of the few documenting multispecies successes and failures in inadvertent plant introductions. Results reveal distinct trends in species establishment and geographic spread and highlight the utility of herbarium specimens in answering questions that span large time scales.
MeSH term(s)	Ships ; Plants ; Introduced Species ; New England ; New Jersey
Language	English
Publishing date	2023-09-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2935-x
ISSN	1537-2197 ; 0002-9122
ISSN (online)	1537-2197
ISSN	0002-9122
DOI	10.1002/ajb2.16224
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Article ; Online: Integrating mechanical signals into cellular identity.

Carley, Emma / King, Megan C / Guo, Shangqin

Trends in cell biology

2022 Volume 32, Issue 8, Page(s) 669–680

Abstract: The large arrays of cell types in a multicellular organism are defined by their stereotypic size and/or morphology, and, for cells in vivo, by their anatomic positions. Historically, this identity-structure-function correlation was conceptualized as ... ...

Abstract	The large arrays of cell types in a multicellular organism are defined by their stereotypic size and/or morphology, and, for cells in vivo, by their anatomic positions. Historically, this identity-structure-function correlation was conceptualized as arising from distinct gene expression programs that dictate how cells appear and behave. However, a growing number of studies suggest that a cell's mechanical state is also an important determinant of its identity, both in lineage-committed cells and in pluripotent stem cells. Defining the mechanism by which mechanical inputs influence complex cellular programs remains an area of ongoing investigation. Here, we discuss how the cytoskeleton actively participates in instructing the response of the nucleus and genome to integrate mechanical and biochemical inputs, with a primary focus on the role of the actomyosin-LINC (linker of nucleoskeleton and cytoskeleton) complex axis.
MeSH term(s)	Actomyosin/metabolism ; Cell Nucleus/metabolism ; Cytoskeleton/metabolism ; Humans ; Mechanotransduction, Cellular/physiology ; Microtubules/metabolism
Chemical Substances	Actomyosin (9013-26-7)
Language	English
Publishing date	2022-03-23
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	30122-x
ISSN	1879-3088 ; 0962-8924
ISSN (online)	1879-3088
ISSN	0962-8924
DOI	10.1016/j.tcb.2022.02.006
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Article ; Online: Going nuclear: Recent developments, cutting-edge tools, and new paradigms.

Belmont, Andrew S / King, Megan C

Current opinion in cell biology

2020 Volume 64, Page(s) iii–v

Language	English
Publishing date	2020-07-13
Publishing country	England
Document type	Editorial
ZDB-ID	1026381-0
ISSN	1879-0410 ; 0955-0674
ISSN (online)	1879-0410
ISSN	0955-0674
DOI	10.1016/j.ceb.2020.06.001
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Article ; Online: Hidden cargo: The impact of historical shipping trade on the recent‐past and contemporary non‐native flora of northeastern United States

Schmidt, Ryan J. / King, Megan R. / Aronson, Myla F. J. / Struwe, Lena

American Journal of Botany. 2023 Sept., v. 110, no. 9 p.e16224-

2023

Abstract: PREMISE: Understanding establishment and spread of non‐native plants is important in the face of a homogenizing global flora. While many studies focus on successful, invasive species, fewer have studied failed plant introductions. Until the early 1900s, ... ...

Abstract	PREMISE: Understanding establishment and spread of non‐native plants is important in the face of a homogenizing global flora. While many studies focus on successful, invasive species, fewer have studied failed plant introductions. Until the early 1900s, large quantities of ship ballast, often containing foreign plant propagules, were deposited in New Jersey (USA). The resulting ballast flora is documented in extensive herbarium records, providing us a unique opportunity to analyze successes and failures of novel plant species introductions. METHODS: We used digitized specimens from 75 herbaria to study 264 non‐native species introduced into New Jersey through 19th century ballast deposition. We used spatial (density‐based clustering; HDBSCAN) and temporal analyses of species retention and geographic spread to quantify disappearance rate, survival, and dispersion through time and define trajectory groups. RESULTS: Four distinct trajectory groups were identified: waif (only present during import; 32% of species), short‐term (disappeared quickly; 20%), established–limited spread (survives locally, 30%), and established–widespread (widespread, 18%). Species disappearance rate was highest during ballast deposition and decreased soon after deposition stopped around 1900. Spatial patterns showed a strong association with 19th century railroads for inland dispersal from ports. The disappearance rate and spatial analyses are robust to herbarium collection bias. CONCLUSIONS: This study using New Jersey as a model is one of the few documenting multispecies successes and failures in inadvertent plant introductions. Results reveal distinct trends in species establishment and geographic spread and highlight the utility of herbarium specimens in answering questions that span large time scales.
Keywords	flora ; herbaria ; imports ; introduced species ; invasive species ; models ; New Jersey
Language	English
Dates of publication	2023-09
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2935-x
ISSN	1537-2197 ; 0002-9122
ISSN (online)	1537-2197
ISSN	0002-9122
DOI	10.1002/ajb2.16224
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Article ; Online: Homology-directed repair involves multiple strand invasion cycles in fission yeast.

Vines, Amanda J / Cox, Kenneth / Leland, Bryan A / King, Megan C

Molecular biology of the cell

2022 Volume 33, Issue 4, Page(s) ar30

Abstract: Homology-directed repair of DNA double-strand breaks (DSBs) represents a highly faithful pathway. Non-crossover repair dominates in mitotically growing cells, likely through a preference for synthesis-dependent strand annealing (SDSA). How homology- ... ...

Abstract	Homology-directed repair of DNA double-strand breaks (DSBs) represents a highly faithful pathway. Non-crossover repair dominates in mitotically growing cells, likely through a preference for synthesis-dependent strand annealing (SDSA). How homology-directed repair mechanism choice is orchestrated in time and space is not well understood. Here, we develop a microscopy-based assay in living fission yeast to determine the dynamics and kinetics of an engineered, site-specific interhomologue repair event. We observe highly efficient homology search and homology-directed repair in this system. Surprisingly, the initial distance between the DSB and the donor sequence does not correlate with the duration of repair. Instead, we observe that repair often involves multiple site-specific and Rad51-dependent colocalization events between the DSB and donor sequence. Upon loss of the RecQ helicase Rqh1 (BLM in humans) we observe rapid repair possibly involving a single strand invasion event, suggesting that multiple strand invasion cycles antagonized by Rqh1 could reflect ongoing SDSA. However, failure to colocalize with the donor sequence and execute repair is also more likely in
MeSH term(s)	DNA Breaks, Double-Stranded ; DNA Helicases/metabolism ; DNA Repair ; DNA Replication ; Humans ; Recombinational DNA Repair ; Schizosaccharomyces/metabolism ; Schizosaccharomyces pombe Proteins/metabolism
Chemical Substances	Schizosaccharomyces pombe Proteins ; DNA Helicases (EC 3.6.4.-) ; Rqh1 protein, S pombe (EC 5.99.-)
Language	English
Publishing date	2022-01-26
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1098979-1
ISSN	1939-4586 ; 1059-1524
ISSN (online)	1939-4586
ISSN	1059-1524
DOI	10.1091/mbc.E20-07-0433
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