LIVIVO - Search results -

Search results

Result 1 - 10 of total 209

Search options

Article: Direct pollination of Zea mays ovules in vitro with Z. mays, Z. mexicana and Sorghum bicolor pollen.

Dhaliwal, S / King, P J

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

2013 Volume 53, Issue 1, Page(s) 43–46

Abstract: Sorghum (Sorghum bicolor) pollen tubes penetrated and grew in corn (Zea mays) styles. The limited length of the sorghum pollen tubes (3-5 mm) and the absence of stigmatic hairs on the basal (5-10 mm) section of the corn styles prevented effective ... ...

Abstract	Sorghum (Sorghum bicolor) pollen tubes penetrated and grew in corn (Zea mays) styles. The limited length of the sorghum pollen tubes (3-5 mm) and the absence of stigmatic hairs on the basal (5-10 mm) section of the corn styles prevented effective pollination in vivo and in vitro. Normal fertilisation occurred after in vitro pollination of exposed corn ovules with either corn or teosinte (Zea mexicana) pollen. Six per cent of corn ovules pollinated directly with sorghum pollen responded by rapid, massive growth of nucellar tissue.
Language	English
Publishing date	2013-12-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2170-2
ISSN	1432-2242 ; 0040-5752
ISSN (online)	1432-2242
ISSN	0040-5752
DOI	10.1007/BF00273135
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1692: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article: Biochemical selection of immature, haploid embryos of Zea mays L.

Dhaliwal, H S / King, P J

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

2013 Volume 55, Issue 6, Page(s) 257–262

Abstract: A method was devised for the biochemical selection of immature, haploid Zea mays embryos using Adh1 (-) and either the Stock 6 or indeterminate gametophyte (ig in W23) high haploid-inducing systems. Haploid (Adh1 (-)) embryos survived exposure to levels ... ...

Abstract	A method was devised for the biochemical selection of immature, haploid Zea mays embryos using Adh1 (-) and either the Stock 6 or indeterminate gametophyte (ig in W23) high haploid-inducing systems. Haploid (Adh1 (-)) embryos survived exposure to levels of allyl alcohol which killed diploid (Adh1 (+)/Adh1 (-)) embryos. Of the total surviving embryos which were examined cytologically 15% (using ig) and 22% (using Stock 6) were haploid. In two experiments with Stock 6, 100% of the surviving embryos were haploid. To obtain maximum effectiveness of Stock 6 and ig, Adh1 (-) was transferred to stock 6 and W23 backgrounds. Immature, haploid embryos are being used to develop haploid, morphogenic tissue cultures of Zea mays.
Language	English
Publishing date	2013-12-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2170-2
ISSN	1432-2242 ; 0040-5752
ISSN (online)	1432-2242
ISSN	0040-5752
DOI	10.1007/BF00265362
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1692: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: A temperature-sensitive auxin auxotroph not deficient in indole-3-acetic acid.

Oetiker, J / Gebhardt, C / King, P J

Planta

2013 Volume 180, Issue 2, Page(s) 220–228

Abstract: A temperature-sensitive variant of Hyoscyamus muticus L. expressing a lethal phenotype in both cultured cells and regenerated plants has been shown to be a conditional auxin auxotroph with an absolute requirement for an exogenous auxin at temperatures ... ...

Abstract	A temperature-sensitive variant of Hyoscyamus muticus L. expressing a lethal phenotype in both cultured cells and regenerated plants has been shown to be a conditional auxin auxotroph with an absolute requirement for an exogenous auxin at temperatures above 30° C but not at lower temperatures. The requirement was satisfied by indole-3-acetic acid (IAA) and all active auxin analogous tested. Despite this dependence on exogenous auxin at high temperatures, the variant nevertheless synthesises IAA under these conditions and contains a pool of free IAA equal to that of the wild type. Furthermore, there is no depletion of the cellular IAA pool on incubation at high temperature in the absence of added auxin, even during the expression of the auxin-auxotrophic phenotype. The characteristics of this variant indicate that auxin auxotrophy can be lethal at the plant level and raises questions about the cellular function and mode of action of auxin.
Language	English
Publishing date	2013-11-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00194000
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus : II. Auxotrophic and temperature-sensitive clones.

Gebhardt, C / Schnebli, V / King, P J

Planta

2013 Volume 153, Issue 1, Page(s) 81–89

Abstract: The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated ...

Abstract	The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two are auxotrophic for histidine, one clone for tryptophan and three clones for nicotinamide. Two clones that grow only in presence of a group of amino acids including glutamine and asparagine are also ClO 3 (-) resistant. Two further clones have as yet undefined amino-acid requirements. Two temperature-sensitive clones were found, one of which stops growing at the restrictive temperature of 32°C and the other undergoes chlorosis and accumulates an insoluble brown pigment. All clones expressed consistently the variant phenotypes in many retests and characterisation experiments over more than one year. Shoots have been regenerated from the nicotinamide- and histidine-requiring clones and from one temperaturesensitive clone. Two control (wild-type) morphogenic clones were used: one green and the other more variably pigmented and showing some growth stimulation in presence of medium supplements.
Language	English
Publishing date	2013-11-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00385321
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Isolation of biochemical mutants using haploid mesophyll protosplasts of Hyoscyamus muticus : IV. Biochemical characterisation of nitrate non-utilizing clones.

Fankhauser, H / Bucher, F / King, P J

Planta

2013 Volume 160, Issue 5, Page(s) 415–421

Abstract: Four nitrate non-utilizing clones of Hyoscyamus muticus, obtained by a total isolation method, possess all the known characteristics of cnx-type nitrate-reductase-deficient variants: 1) strict dependence on a reduced nitrogen source such as a mixture of ... ...

Abstract	Four nitrate non-utilizing clones of Hyoscyamus muticus, obtained by a total isolation method, possess all the known characteristics of cnx-type nitrate-reductase-deficient variants: 1) strict dependence on a reduced nitrogen source such as a mixture of amino acids; 2) chlorate resistance; 3) normal nitrate uptake; 4) lack of nitrate-reductase and xanthine-dehydrogenase activities, but presence of cytochrome-c-reductase and nitrite-reductase activities; 5) in vitro nitrate-reductase complementation with a molybdenum cofactor source. Two of the clones (MA-2 and I2D12) are molybdate repairable in vivo, whilst the other two clones (VIC2 and XIVE9) are not.
Language	English
Publishing date	2013-11-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00429757
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus : I. A NO 3 (-) non-utilizing clone.

Strauss, A / Bucher, F / King, P J

Planta

2013 Volume 153, Issue 1, Page(s) 75–80

Abstract: A population of 3070 clones derived from N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-treated mesophyll protoplasts of haploid Hyoscyamus muticus was tested for amino-acid auxotrophy without enrichment. One clone (MA-2) was stably and specifically ... ...

Abstract	A population of 3070 clones derived from N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-treated mesophyll protoplasts of haploid Hyoscyamus muticus was tested for amino-acid auxotrophy without enrichment. One clone (MA-2) was stably and specifically dependent on casein hydrolysate and could be fed also by a number of single amino acids or by other reduced nitrogen sources. MA-2 was found to be chlorate resistant and devoid of in vivo nitrate reductase activity under inductive conditions. Permissive and restrictive growth conditions for MA-2 were investigated more closely and media were found promoting morphogenesis. Selection and testing of clones were complicated by an unspecific growth stimulation of some wild type cultures by amino acids, thiamine and m-inositol.
Language	English
Publishing date	2013-11-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00385320
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Isolation and cryopreservation of O-methylthreonine-resistant Rosa cell lines altered in the feedback sensitivity of L-threonine deaminase.

Strauss, A / Fankhauser, H / King, P J

Planta

2013 Volume 163, Issue 4, Page(s) 554–562

Abstract: O-Methylthreonine (OMT) inhibits the growth of plated Rosa cells (ID50≃6·10(-6)M). Isoleucine is able to reverse efficiently and specifically this OMT toxicity. From OMT-resistant colonies occurring at a frequency of 1.58·10(-7) variants per cell plated ... ...

Abstract	O-Methylthreonine (OMT) inhibits the growth of plated Rosa cells (ID50≃6·10(-6)M). Isoleucine is able to reverse efficiently and specifically this OMT toxicity. From OMT-resistant colonies occurring at a frequency of 1.58·10(-7) variants per cell plated at 10(-4)M OMT, the variant strains OMT(R)-1 and OMT(R)-2 were isolated, cloned via protoplasts and characterized. Both variants were ten times more resistant to OMT than the wildtype and were cross-resistant to another isoleucine analog, DL-4-thiaisoleucine. The resistant variants retained their resistance after storage for three years in liquid nitrogen. Both resistant strains were stable for several months when subcultured in the absence of OMT although it was shown in a reconstitution experiment that wildtype cells overgrow OMT(R)-2 variant cells if co-cultivated for many passages in drug-free medium. One case of instability was observed upon long-term subculturing in drug-free medium: the strain OMT(R)-1D(*) partially lost phenotypic properties. Resistance to OMT was followed qualitatively by a new method based on inhibition-zone formation in cell suspensions plated in agar medium. The OMT-resistant variants showed a reduction in sensitivity of the enzyme L-threonine deaminase to feedback inhibition by isoleucine, a decreased stability of L-threonine deaminase when stored at-18°C or incubated at +55°C and a two- to threefold increase of the free isoleucine pool within the cells. The genetical events and the biochemical mechanisms which might lead to the observed stable and biochemically defined character are discussed with particular reference to the high ploidy level of the Rosa cell line.
Language	English
Publishing date	2013-11-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00392713
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Metabolic events in synchronised cell cultures of Acer pseudoplatanus L.

King, P J / Cox, B J / Fowler, M W / Street, H E

Planta

2014 Volume 117, Issue 2, Page(s) 109–122

Abstract: Cell division was synchronised in 4-litre batch cultures of Acer pseudoplatanus L. by starvation and regrowth. Up to five consecutive cell cycles were observed in each culture. Mitosis and cytokinesis were synchronised within 0.2 cell cycles. ... ...

Abstract	Cell division was synchronised in 4-litre batch cultures of Acer pseudoplatanus L. by starvation and regrowth. Up to five consecutive cell cycles were observed in each culture. Mitosis and cytokinesis were synchronised within 0.2 cell cycles. Accumulation of extractable DNA was discontinuous and separate from cytokinesis. Correction for the degree of synchrony in the population gave: G1=13-37 h, S=15 h, G2=14-19 h and M=0.9-1.3 h. Thymidine kinase activity and [(14)C]thymidine incorporation were highest during S-phase. A peak of activity of aspartate transcarbamoylase occurred during G2. Peaks in succinate dehydrogenase activity and respiration rate were observed at the initiation of DNA synthesis and just prior to mitosis. The activity of glucose-6-phosphate dehydrogenase doubled in one step during the cell cycle. Total RNA and protein accumulated continuously through the cell cycle; the final rate being twice that observed initially.
Language	English
Publishing date	2014-01-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00390793
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: A cytokinin-resistant mutant of Nicotiana plumbaginifolia is wilty.

Blonstein, A D / Parry, A D / Horgan, R / King, P J

Planta

2013 Volume 183, Issue 2, Page(s) 244–250

Abstract: Selection for cytokinin resistance by incubating M2 seed of Nicotiana plumbaginifolia, after ethylmethanesulphonate mutagenesis, on 20 μM 6-benzylami-nopurine resulted in the isolation of a monogenic, recessive mutant, CKR1. Germination of the mutant is ... ...

Abstract	Selection for cytokinin resistance by incubating M2 seed of Nicotiana plumbaginifolia, after ethylmethanesulphonate mutagenesis, on 20 μM 6-benzylami-nopurine resulted in the isolation of a monogenic, recessive mutant, CKR1. Germination of the mutant is less sensitive to cytokinin inhibition than the wild type, and leaf development of the mutant occurs at cytokinin concentrations inhibitory to the wild type. Germination of the mutant is also resistant to auxin but not to abscisic acid. Three other traits jointly inherited with cytokinin resistance in the F2 are lack of root branching, precocious germination and wiltiness. The wilty phenotype is the consequence of the failure of stomatal closure during water stress.
Language	English
Publishing date	2013-11-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00197795
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 46/303: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Proline: A Novel Cryoprotectant for the Freeze Preservation of Cultured Cells of Zea mays L.

Withers, L A / King, P J

Plant physiology

2006 Volume 64, Issue 5, Page(s) 675–678

Abstract: Proline is an effective cryoprotectant for the storage of cultured cells of Zea mays L. in liquid N(2). Increased freeze tolerance can be achieved by pregrowth for 3 to 4 days in medium containing proline. Cells cryoprotected with proline have an ... ...

Abstract	Proline is an effective cryoprotectant for the storage of cultured cells of Zea mays L. in liquid N(2). Increased freeze tolerance can be achieved by pregrowth for 3 to 4 days in medium containing proline. Cells cryoprotected with proline have an increased recovery potential when compared with cells cryoprotected with dimethylsulfoxide and glycerol. They also show a reduced postthaw viability loss and greater tolerance of a range of postthaw culture conditions. It is suggested that the mechanism of action of proline may be similar to that in its putative role of conferring protection against natural stresses. It may be protecting the cell against solution effects caused by dehydration during freezing. These findings are discussed in relation to other freeze tolerance enhancing treatments.
Language	English
Publishing date	2006-04-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	208914-2
ISSN	1532-2548 ; 0032-0889
ISSN (online)	1532-2548
ISSN	0032-0889
DOI	10.1104/pp.64.5.675
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 1193: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED