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Artikel ; Online: Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation.

Miloro, Fabio / Kis, András / Havelda, Zoltán / Dalmadi, Ágnes

2024 Band 43, Heft 4, Seite(n) 96

Abstract: Key message: Barley AGO4 proteins complement expressional changes of epigenetically regulated genes in Arabidopsis ago4-3 mutant and show a distinct affinity for the 5' terminal nucleotide of small RNAs, demonstrating functional conservation and ... ...

Abstract	Key message: Barley AGO4 proteins complement expressional changes of epigenetically regulated genes in Arabidopsis ago4-3 mutant and show a distinct affinity for the 5' terminal nucleotide of small RNAs, demonstrating functional conservation and divergence. The function of Argonaute 4 (AGO4) in Arabidopsis thaliana has been extensively characterized; however, its role in monocots, which have large genomes abundantly supplemented with transposable elements (TEs), remains elusive. The study of barley AGO4 proteins can provide insights into the conserved aspects of RNA-directed DNA methylation (RdDM) and could also have further applications in the field of epigenetics or crop improvement. Bioinformatic analysis of RNA sequencing data identified two active AGO4 genes in barley, HvAGO4a and HvAGO4b. These genes function similar to AtAGO4 in an Arabidopsis heterologous complementation system, primarily binding to 24-nucleotide long small RNAs (sRNAs) and triggering methylation at specific target loci. Like AtAGO4, HvAGO4B exhibits a preference for binding sRNAs with 5' adenine residue, while also accepting 5' guanine, uracil, and cytosine residues. In contrast, HvAGO4A selectively binds only sRNAs with a 5' adenine residue. The diverse binding capacity of barley AGO4 proteins is reflected in TE-derived sRNAs and in their varying abundance. Both barley AGO4 proteins effectively restore the levels of extrachromosomal DNA and transcript abundancy of the heat-activated ONSEN retrotransposon to those observed in wild-type Arabidopsis plants. Our study provides insight into the distinct binding specificities and involvement in TE regulation of barley AGO4 proteins in Arabidopsis by heterologous complementation.
Mesh-Begriff(e)	Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Hordeum/genetics ; Hordeum/metabolism ; RNA, Small Interfering/genetics ; Nucleotides/metabolism ; Adenine/metabolism ; DNA Methylation/genetics ; RNA, Plant/genetics
Chemische Substanzen	Arabidopsis Proteins ; RNA, Small Interfering ; Nucleotides ; Adenine (JAC85A2161) ; RNA, Plant
Sprache	Englisch
Erscheinungsdatum	2024-03-13
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	8397-5
ISSN	1432-203X ; 0721-085X ; 0721-7714
ISSN (online)	1432-203X
ISSN	0721-085X ; 0721-7714
DOI	10.1007/s00299-024-03177-z
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Reducing flood risk by effective use of flood‐peak polders: A case study of the Tisza River

Ungvári, Gábor / Kis, András

Journal of flood risk management. 2022 Sept., v. 15, no. 3

2022

Abstract: Between 1998 and 2006 a series of extreme flood events took place on the Tisza River and its tributaries. In Hungary, this triggered the development of flood‐peak polders as a more cost‐efficient solution of defense compared to raising the dikes. The ... ...

Abstract	Between 1998 and 2006 a series of extreme flood events took place on the Tisza River and its tributaries. In Hungary, this triggered the development of flood‐peak polders as a more cost‐efficient solution of defense compared to raising the dikes. The recent analysis applies Monte‐Carlo simulation‐based quantified risk calculations with a cost–benefit type comparison. Results indicate that compared to the originally planned, 100‐year return frequency flood that threats to topple the levees, lower flood levels already provide economic justification for polder use. Apart from the optimal timing of opening the floodgates, the controlled inundation of polders requires the consideration of its cost–benefit effects as well. The development of the economic decision‐support system for the controlled use of the flood‐peak polders along the Tisza River provides an insight into the efficiency gains that a more informed, quantitative economic analysis can offer in risk reduction. The analysis reveals the potential for more efficient management of flood polders. The decision support of controlled polder inundation includes all the necessary information elements for the cross‐sectoral comparability of impacts that is the foundation for any multi‐purpose land management scheme that enables nature‐based solutions.
Schlagwörter	case studies ; cost effectiveness ; decision support systems ; polders ; risk ; risk reduction ; rivers ; Hungary
Sprache	Englisch
Erscheinungsverlauf	2022-09
Erscheinungsort	Blackwell Publishing Ltd
Dokumenttyp	Artikel
Anmerkung	JOURNAL ARTICLE
ZDB-ID	2430376-8
ISSN	1753-318X
ISSN	1753-318X
DOI	10.1111/jfr3.12823
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids.

Mihók, Edit / Polgári, Dávid / Lenykó-Thegze, Andrea / Makai, Diána / Fábián, Attila / Ali, Mohammad / Kis, András / Sepsi, Adél / Sági, László

Frontiers in plant science

2024 Band 15, Seite(n) 1324817

Abstract: Incorporating the centromere-specific histone H3 protein CENH3 into the centromeric nucleosomes is indispensable for accurate centromere function and balanced chromosome segregation in most eukaryotes, including higher plants. In the cell nuclei of ... ...

Abstract	Incorporating the centromere-specific histone H3 protein CENH3 into the centromeric nucleosomes is indispensable for accurate centromere function and balanced chromosome segregation in most eukaryotes, including higher plants. In the cell nuclei of interspecific hybrids, divergent centromeric DNAs cohabit and lead the corresponding parental chromosomes through the mitotic and meiotic cell divisions. Depending on the transmission of the parental chromosomes carrying the CENH3-encoding genes, CENH3 proteins from one or both parents may be present in these hybrids. The incorporation of parental CENH3 proteins into the divergent centromeres and their role in the chromosome elimination process in interspecific hybrids is still poorly understood. Here, we produced wheat × barley F1 hybrids that carried different combinations of barley chromosomes with genes encoding for either one (αCENH3) or both barley CENH3 protein variants (α- and βCENH3). We generated specific antibodies distinguishing between the wheat CENH3 proteins and barley αCENH3 and applied them together with FISH probes to detect the precise pattern of parental CENH3 deposition into the wheat and barley centromeric nucleosomes. Analysis of somatic and meiotic nuclei of the wheat × barley hybrids revealed the plasticity of the maternal (wheat) CENH3 proteins to become incorporated into the paternal (barley) centromeric nucleosomes. However, no evidence for paternal CENH3 plasticity was detected in this study. The significance of the unilateral centromere plasticity and possible patterns of CENH3 incorporation into centromeres in interspecific hybrids are discussed.
Sprache	Englisch
Erscheinungsdatum	2024-01-19
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2024.1324817
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Correction: Rapid and cost-effective molecular karyotyping in wheat, barley, and their crossprogeny by chromosome-specific multiplex PCR.

Ali, Mohammad / Polgári, Dávid / Sepsi, Adél / Kontra, Levente / Dalmadi, Ágnes / Havelda, Zoltán / Sági, László / Kis, András

Plant methods

2024 Band 20, Heft 1, Seite(n) 55

Sprache	Englisch
Erscheinungsdatum	2024-04-20
Erscheinungsland	England
Dokumenttyp	Published Erratum
ZDB-ID	2203723-8
ISSN	1746-4811
ISSN	1746-4811
DOI	10.1186/s13007-024-01179-2
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR.

Ali, Mohammad / Polgári, Dávid / Sepsi, Adél / Kontra, Levente / Dalmadi, Ágnes / Havelda, Zoltán / Sági, László / Kis, András

Plant methods

2024 Band 20, Heft 1, Seite(n) 37

Abstract: Background: Interspecific hybridisation is a powerful tool for increasing genetic diversity in plant breeding programmes. Hexaploid wheat (Triticum aestivum, 2n = 42) × barley (Hordeum vulgare, 2n = 14) intergeneric hybrids can contribute to the ... ...

Abstract	Background: Interspecific hybridisation is a powerful tool for increasing genetic diversity in plant breeding programmes. Hexaploid wheat (Triticum aestivum, 2n = 42) × barley (Hordeum vulgare, 2n = 14) intergeneric hybrids can contribute to the transfer of agronomically useful traits by creating chromosome addition or translocation lines as well as full hybrids. Information on the karyotype of hybrid progenies possessing various combinations of wheat and barley chromosomes is thus essential for the subsequent breeding steps. Since the standard technique of chromosome in situ hybridisation is labour-intensive and requires specific skills. a routine, cost-efficient, and technically less demanding approach is beneficial both for research and breeding. Results: We developed a Multiplex Polymerase Chain Reaction (MPCR) method to identify individual wheat and barley chromosomes. Chromosome-specific primer pairs were designed based on the whole genome sequences of 'Chinese Spring' wheat and 'Golden Promise' barley as reference cultivars. A pool of potential primers was generated by applying a 20-nucleotide sliding window with consecutive one-nucleotide shifts on the reference genomes. After filtering for optimal primer properties and defined amplicon sizes to produce an ordered ladder-like pattern, the primer pool was manually curated and sorted into four MPCR primer sets for the wheat A, B, and D sub-genomes, and for the barley genome. The designed MPCR primer sets showed high chromosome specificity in silico for the genome sequences of all 18 wheat and barley cultivars tested. The MPCR primers proved experimentally also chromosome-specific for the reference cultivars as well as for 13 additional wheat and four barley genotypes. Analyses of 16 wheat × barley F1 hybrid plants demonstrated that the MPCR primer sets enable the fast and one-step detection of all wheat and barley chromosomes. Finally, the established genotyping system was fully corroborated with the standard genomic in situ hybridisation (GISH) technique. Conclusions: Wheat and barley chromosome-specific MPCR offers a fast, labour-friendly, and versatile alternative to molecular cytogenetic detection of individual chromosomes. This method is also suitable for the high-throughput analysis of distinct (sub)genomes, and, in contrast to GISH, can be performed with any tissue type. The designed primer sets proved to be highly chromosome-specific over a wide range of wheat and barley genotypes as well as in wheat × barley hybrids. The described primer design strategy can be extended to many species with precise genome sequence information.
Sprache	Englisch
Erscheinungsdatum	2024-03-05
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2203723-8
ISSN	1746-4811
ISSN	1746-4811
DOI	10.1186/s13007-024-01162-x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: The Feasibility of Cooperation to Comply with Land Use Change Obligations in the Marosszög Area of South Hungary

Ungvári Gábor / Jolánkai Zsolt / Kis András / Kozma Zsolt

Journal of Environmental Geography, Vol 11, Iss 3-4, Pp 37-

2018 Band 47

Abstract: In many years excess water inundations generate a major obstacle to farming in the lowland part of Hungary, including the Marosszög area. Diverting water to large distances requires an infrastructure that is costly to develop and maintain. Alternatively, ...

Abstract	In many years excess water inundations generate a major obstacle to farming in the lowland part of Hungary, including the Marosszög area. Diverting water to large distances requires an infrastructure that is costly to develop and maintain. Alternatively, low-lying local land segments could be withdrawn from cultivation and utilized to collect the surplus water. The Ecological Focus Area (EFA) requirement of the EU points to the same direction: it requires that 5% of arable land is converted to other, ecologically more beneficial uses. During the research project it was tested if it is feasible to apply a novel economic policy instrument, an auction to trade land use change obligations, to achieve the EFA requirement in a cost effective way through the cooperation of farmers, while also creating a practical solution to manage the seasonal surplus water cover on land. The research was carried out in an interdisciplinary way: a dynamically coupled fully integrated hydrological model, including surface and subsurface modules, was applied by engineers to better understand the interconnections of land use, local hydrology and the role of the water diversion infrastructure; while a pilot auction exercise was conducted by economists with the participation of farmers to understand if cost reductions can be achieved through cooperation, as opposed to individual fulfilment of EFA obligations. The analysis also revealed which segments of the water diversion network are economic to maintain. It was confirmed that it is possible to improve local water management and satisfy the EFA requirements at a reduced cost if appropriate economic incentives are applied to trigger the cooperation of farmers.
Schlagwörter	inland excess water ; land use adaptation ; agriculture ; economic instruments ; water management infrastructure ; auction ; Environmental sciences ; GE1-350
Thema/Rubrik (Code)	333
Sprache	Englisch
Erscheinungsdatum	2018-11-01T00:00:00Z
Verlag	University of Szeged
Dokumenttyp	Artikel ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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Artikel ; Online: Electrically tunable dipolar interactions between layer-hybridized excitons.

Erkensten, Daniel / Brem, Samuel / Perea-Causín, Raül / Hagel, Joakim / Tagarelli, Fedele / Lopriore, Edoardo / Kis, Andras / Malic, Ermin

Nanoscale

2023 Band 15, Heft 26, Seite(n) 11064–11071

Abstract: Transition-metal dichalcogenide bilayers exhibit a rich exciton landscape including layer-hybridized excitons, ...

Abstract	Transition-metal dichalcogenide bilayers exhibit a rich exciton landscape including layer-hybridized excitons,
Sprache	Englisch
Erscheinungsdatum	2023-07-06
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/d3nr01049j
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Electrical detection of the flat-band dispersion in van der Waals field-effect structures.

Pasquale, Gabriele / Lopriore, Edoardo / Sun, Zhe / Čerņevičs, Kristiāns / Tagarelli, Fedele / Watanabe, Kenji / Taniguchi, Takashi / Yazyev, Oleg V / Kis, Andras

Nature nanotechnology

2023 Band 18, Heft 12, Seite(n) 1416–1422

Abstract: Two-dimensional flat-band systems have recently attracted considerable interest due to the rich physics unveiled by emergent phenomena and correlated electronic states at van Hove singularities. However, the difficulties in electrically detecting the ... ...

Abstract	Two-dimensional flat-band systems have recently attracted considerable interest due to the rich physics unveiled by emergent phenomena and correlated electronic states at van Hove singularities. However, the difficulties in electrically detecting the flat-band position in field-effect structures are slowing down the investigation of their properties. In this work, we use indium selenide (InSe) as a flat-band system due to a van Hove singularity at the valence-band edge in a few-layer form of the material without the requirement of a twist angle. We investigate tunnelling photocurrents in gated few-layer InSe structures and relate them to ambipolar transport and photoluminescence measurements. We observe an appearance of a sharp change in tunnelling mechanisms due to the presence of the van Hove singularity at the flat band. We further corroborate our findings by studying tunnelling currents as a reliable probe for the flat-band position up to room temperature. Our results create an alternative approach to studying flat-band systems in heterostructures of two-dimensional materials.
Sprache	Englisch
Erscheinungsdatum	2023-08-17
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2254964-X
ISSN	1748-3395 ; 1748-3387
ISSN (online)	1748-3395
ISSN	1748-3387
DOI	10.1038/s41565-023-01489-x
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Disorder-induced bulk photovoltaic effect in a centrosymmetric van der Waals material.

Cheon, Cheol-Yeon / Sun, Zhe / Cao, Jiang / Gonzalez Marin, Juan Francisco / Tripathi, Mukesh / Watanabe, Kenji / Taniguchi, Takashi / Luisier, Mathieu / Kis, Andras

NPJ 2D materials and applications

2023 Band 7, Heft 1, Seite(n) 74

Abstract: Sunlight is widely seen as one of the most abundant forms of renewable energy, with photovoltaic cells based on pn junctions being the most commonly used platform attempting to harness it. Unlike in conventional photovoltaic cells, the bulk photovoltaic ... ...

Abstract	Sunlight is widely seen as one of the most abundant forms of renewable energy, with photovoltaic cells based on pn junctions being the most commonly used platform attempting to harness it. Unlike in conventional photovoltaic cells, the bulk photovoltaic effect (BPVE) allows for the generation of photocurrent and photovoltage in a single material without the need to engineer a pn junction and create a built-in electric field, thus offering a solution that can potentially exceed the Shockley-Queisser efficiency limit. However, it requires a material with no inversion symmetry and is therefore absent in centrosymmetric materials. Here, we demonstrate that breaking the inversion symmetry by structural disorder can induce BPVE in ultrathin PtSe
Sprache	Englisch
Erscheinungsdatum	2023-11-21
Erscheinungsland	England
Dokumenttyp	Journal Article
ISSN	2397-7132
ISSN (online)	2397-7132
DOI	10.1038/s41699-023-00435-8
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Targeted mutations in the GW2.1 gene modulate grain traits and induce yield loss in barley.

Kis, András / Polgári, Dávid / Dalmadi, Ágnes / Ahmad, Imtiaz / Rakszegi, Marianna / Sági, László / Csorba, Tibor / Havelda, Zoltán

Plant science : an international journal of experimental plant biology

2023 Band 340, Seite(n) 111968

Abstract: Grain Width and Weight 2 (GW2) is an E3-ubiquitin ligase-encoding gene that negatively regulates the size and weight of the grain in cereal species. Therefore, disabling GW2 gene activity was suggested for enhancing crop productivity. We show here that ... ...

Abstract	Grain Width and Weight 2 (GW2) is an E3-ubiquitin ligase-encoding gene that negatively regulates the size and weight of the grain in cereal species. Therefore, disabling GW2 gene activity was suggested for enhancing crop productivity. We show here that CRISPR/Cas-mediated mutagenesis of the barley GW2.1 homologue results in the development of elongated grains and increased protein content. At the same time, GW2.1 loss of function induces a significant grain yield deficit caused by reduced spike numbers and low grain setting. We also show that the converse effect caused by GW2.1 absence on crop yield and protein content is largely independent of cultivation conditions. These findings indicate that the barley GW2.1 gene is necessary for the optimization between yield and grain traits. Altogether, our data show that the loss of GW2.1 gene activity in barley is associated with pleiotropic effects negatively affecting the development of generative organs and consequently the grain production. Our findings contribute to the better understanding of grain development and the utilisation of GW2.1 control in quantitative and qualitative genetic improvement of barley.
Mesh-Begriff(e)	Edible Grain/genetics ; Hordeum/genetics ; Phenotype ; Mutation
Sprache	Englisch
Erscheinungsdatum	2023-12-27
Erscheinungsland	Ireland
Dokumenttyp	Journal Article
ZDB-ID	742010-9
ISSN	1873-2259 ; 0168-9452
ISSN (online)	1873-2259
ISSN	0168-9452
DOI	10.1016/j.plantsci.2023.111968
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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