LIVIVO - Search results -

Search results

Result 1 - 10 of total 19

Search options

Article: Patient-Derived Organoids of Colorectal Cancer: A Useful Tool for Personalized Medicine.

Kiwaki, Takumi / Kataoka, Hiroaki

2022 Volume 12, Issue 5

Abstract: Colorectal cancer is one of the most important malignancies worldwide, with high incidence and mortality rates. Several studies have been conducted using two-dimensional cultured cell lines; however, these cells do not represent a study model of patient ... ...

Abstract	Colorectal cancer is one of the most important malignancies worldwide, with high incidence and mortality rates. Several studies have been conducted using two-dimensional cultured cell lines; however, these cells do not represent a study model of patient tumors very well. In recent years, advancements in three-dimensional culture methods have facilitated the establishment of patient-derived organoids, which have become indispensable for molecular biology-related studies of colorectal cancer. Patient-derived organoids are useful in both basic science and clinical practice; they can help predict the sensitivity of patients with cancer to chemotherapy and radiotherapy and provide the right treatment to the right patient. Regarding precision medicine, combining gene panel testing and organoid-based screening can increase the effectiveness of medical care. In this study, we review the development of three-dimensional culture methods and present the most recent information on the clinical application of patient-derived organoids. Moreover, we discuss the problems and future prospects of organoid-based personalized medicine.
Language	English
Publishing date	2022-04-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662248-8
ISSN	2075-4426
ISSN	2075-4426
DOI	10.3390/jpm12050695
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium

Kawaguchi, Makiko / Kataoka, Hiroaki / Kiwaki, Takumi / Weiting, Liang / Nagata, Sayaka / Kitamura, Kazuo / Fukushima, Tsuyoshi

FEBS Open Bio. 2023 Apr., v. 13, no. 4, p. 713-723

2023 , Page(s) 713–723

Abstract: Adrenomedullin (AM) is a peptide with pleiotropic physiological functions that attenuates intestinal mucosal inflammation. However, the mechanism underpinning mucosal protection by AM is not fully understood, and its effect on intestinal epithelial cells ...

Abstract	Adrenomedullin (AM) is a peptide with pleiotropic physiological functions that attenuates intestinal mucosal inflammation. However, the mechanism underpinning mucosal protection by AM is not fully understood, and its effect on intestinal epithelial cells remains unclear. Here, we investigated the effects of AM on junctional molecules in primary‐cultured murine intestinal epithelial cells and discovered that AM upregulates claudin‐4 expression. In a mouse model of dextran sulfate sodium‐induced colitis, AM administration also enhanced claudin‐4 expression and accelerated mucosal regeneration. Furthermore, AM reversed TNFα‐mediated downregulation of claudin‐4 and loss of cell–cell adhesion of the HCT116 human intestinal epithelial cell line in vitro. These results indicate that AM may enhance intestinal epithelial integrity by upregulating claudin‐4 expression.
Keywords	cell adhesion ; cell lines ; colitis ; dextran sulfate ; epithelial cells ; epithelium ; humans ; inflammation ; intestines ; mice ; models ; peptides
Language	English
Dates of publication	2023-04
Size	p. 713-723
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2651702-4
ISSN	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13577
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin-4 expression in the colonic epithelium.

Kawaguchi, Makiko / Kataoka, Hiroaki / Kiwaki, Takumi / Weiting, Liang / Nagata, Sayaka / Kitamura, Kazuo / Fukushima, Tsuyoshi

FEBS open bio

2023 Volume 13, Issue 4, Page(s) 713–723

Abstract	Adrenomedullin (AM) is a peptide with pleiotropic physiological functions that attenuates intestinal mucosal inflammation. However, the mechanism underpinning mucosal protection by AM is not fully understood, and its effect on intestinal epithelial cells remains unclear. Here, we investigated the effects of AM on junctional molecules in primary-cultured murine intestinal epithelial cells and discovered that AM upregulates claudin-4 expression. In a mouse model of dextran sulfate sodium-induced colitis, AM administration also enhanced claudin-4 expression and accelerated mucosal regeneration. Furthermore, AM reversed TNFα-mediated downregulation of claudin-4 and loss of cell-cell adhesion of the HCT116 human intestinal epithelial cell line in vitro. These results indicate that AM may enhance intestinal epithelial integrity by upregulating claudin-4 expression.
MeSH term(s)	Mice ; Humans ; Animals ; Adrenomedullin/adverse effects ; Adrenomedullin/metabolism ; Claudin-4 ; Colitis/chemically induced ; Epithelium/metabolism
Chemical Substances	Adrenomedullin (148498-78-6) ; Claudin-4
Language	English
Publishing date	2023-02-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2651702-4
ISSN	2211-5463 ; 2211-5463
ISSN (online)	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13577
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Primary cardiac angiosarcoma directly invading the right lung: An autopsy report.

Kiwaki, Takumi / Ishihara, Akira / Toyama, Takanori / Kusaka, Hiroaki / Kataoka, Hiroaki

Clinical case reports

2021 Volume 9, Issue 8, Page(s) e04582

Abstract: We presented a difficult-to-diagnose case of cardiac angiosarcoma. The patient presented pericardial effusion, but cytology of the effusion was negative. Because cytological detection of angiosarcoma cells is difficult, a possibility of malignancy should ...

Abstract	We presented a difficult-to-diagnose case of cardiac angiosarcoma. The patient presented pericardial effusion, but cytology of the effusion was negative. Because cytological detection of angiosarcoma cells is difficult, a possibility of malignancy should not be excluded with negative cytological examination. Biopsy of cardiac mass is the best way for diagnosis.
Language	English
Publishing date	2021-08-21
Publishing country	England
Document type	Case Reports
ZDB-ID	2740234-4
ISSN	2050-0904
ISSN	2050-0904
DOI	10.1002/ccr3.4582
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Epithelioid Angiomyolipoma with Tumor Thrombus into Inferior Vena Cava Presurgically Treated with Combination Therapy of Pembrolizumab and Axitinib: A Case Report.

Kawasoe, Chihiro / Miyamoto, Yoshi / Ito, Kaoru / Murashima, Takaya / Nagai, Takahiro / Takamori, Hiroki / Kiwaki, Takumi / Kamimura, Toshio / Mukai, Shoichiro / Kamoto, Toshiyuki

Research and reports in urology

2023 Volume 15, Page(s) 447–452

Abstract: Epithelioid angiomyolipoma (EAML) is a rare variant of AML with malignant potential. It is occasionally difficult to distinguish EAML from renal cell carcinoma (RCC) on imaging. A 72-year-old woman was admitted to our hospital for the treatment of a left ...

Abstract	Epithelioid angiomyolipoma (EAML) is a rare variant of AML with malignant potential. It is occasionally difficult to distinguish EAML from renal cell carcinoma (RCC) on imaging. A 72-year-old woman was admitted to our hospital for the treatment of a left renal tumor with relatively high blood flow and a tumor thrombus extending to the inferior vena cava, suggesting RCC. The patient underwent presurgical combination therapy with axitinib and pembrolizumab. This treatment significantly shortened the thrombus, and radical nephrectomy was performed. The pathological findings were compatible with EAML, and the treatment effects were observed. We report a case treated pre-surgically with a combined therapy of pembrolizumab and axitinib, with a favorable response as a treatment option for EAML.
Language	English
Publishing date	2023-10-05
Publishing country	England
Document type	Case Reports
ZDB-ID	2649530-2
ISSN	2253-2447
ISSN	2253-2447
DOI	10.2147/RRU.S425887
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Possible role of combined therapy targeting MET and pro-HGF activation for renal cell carcinoma: analysis by human HGF-producing SCID mice.

Fujii, Masato / Akioka, Takahiro / Kimura, Shoichi / Nagai, Takahiro / Kiwaki, Takumi / Fukushima, Tsuyoshi / Mukai, Shoichiro / Kamoto, Toshiyuki

Human cell

2023 Volume 36, Issue 2, Page(s) 775–785

Abstract: MET is a high-affinity receptor tyrosine kinase of HGF (hepatocyte growth factor). HGF is secreted as an inactive single-chain precursor (pro-HGF), which requires proteolytic activation for conversion to an active form. HGF activator inhibitor (HAI)-2 is ...

Abstract	MET is a high-affinity receptor tyrosine kinase of HGF (hepatocyte growth factor). HGF is secreted as an inactive single-chain precursor (pro-HGF), which requires proteolytic activation for conversion to an active form. HGF activator inhibitor (HAI)-2 is a transmembrane Kunitz-type serine protease inhibitor, which inhibits all pro-HGF-activating enzymes. In RCC, increased expression of MET and decreased expression of HAI-2 were reported to be poor prognostic factors. In the current study, we tried to inhibit the growth of RCC cells by dual inhibition of both MET phosphorylation and pro-HGF-activation using MET inhibitor and HAI-2 overexpression. A transgenic mouse model which expressed human HGF (HGF mouse) was used for in vivo analysis to evaluate the HGF/MET signaling axis accurately. Initially, doxycycline-induced HAI-2 overexpression RCC cells (786-O-HAI2) were prepared. The cells were cultured with pro-HGF, and inhibitory effect of MET inhibitor (SCC244) and HAI-2 was evaluated by phosphorylation of MET and cell proliferation. Next, the cells were subcutaneously implanted to HGF mice and the growth inhibition was determined by SCC244 and HAI-2. Single use of each inhibitor showed significant inhibition in MET phosphorylation, migration and proliferation of 786-O-HAI2 cells; however, the strongest effect was observed by combined use of both inhibitors. Although in vivo analysis also showed apparent downregulation of MET phosphorylation and growth inhibition in combined treatment, statistical significance was not observed compared with single use of MET inhibitor. Combined treatment with MET-TKI and HAI-2 suggested to consider as a candidate for new strong therapy for RCC.
MeSH term(s)	Humans ; Animals ; Mice ; Carcinoma, Renal Cell ; Hepatocyte Growth Factor/metabolism ; Mice, SCID ; Membrane Glycoproteins/metabolism ; Serine Endopeptidases/metabolism ; Trypsin Inhibitor, Kunitz Soybean/metabolism ; Proteinase Inhibitory Proteins, Secretory/metabolism ; Kidney Neoplasms ; Proto-Oncogene Proteins c-met/metabolism
Chemical Substances	pro-hepatocyte growth factor ; Hepatocyte Growth Factor (67256-21-7) ; Membrane Glycoproteins ; Serine Endopeptidases (EC 3.4.21.-) ; Trypsin Inhibitor, Kunitz Soybean (9088-41-9) ; Proteinase Inhibitory Proteins, Secretory ; Proto-Oncogene Proteins c-met (EC 2.7.10.1) ; HGF protein, human
Language	English
Publishing date	2023-01-28
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1149134-6
ISSN	1749-0774 ; 0914-7470
ISSN (online)	1749-0774
ISSN	0914-7470
DOI	10.1007/s13577-023-00857-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3676: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Malignant pheochromocytoma with liver invasion treated successfully by combined retroperitoneal laparoscopic control of arterial in-flow followed by open hepatectomy: A case report.

Otsuka, Isamu / Kida, Kazutaka / Terada, Naoki / Kiwaki, Takumi / Nanashima, Atsushi / Kamoto, Toshiyuki

International journal of surgery case reports

2021 Volume 81, Page(s) 105763

Abstract: Introduction: Pheochromocytoma surgery is generally challenging for surgeons and anesthesiologists for cardiovascular complications.: Presentation of case: A 54-year-old Japanese man was found to have a large right pheochromocytoma infiltrating the ... ...

Abstract	Introduction: Pheochromocytoma surgery is generally challenging for surgeons and anesthesiologists for cardiovascular complications. Presentation of case: A 54-year-old Japanese man was found to have a large right pheochromocytoma infiltrating the posterior part of his liver and vena cava and multiple lung metastases. After retroperitoneal laparoscopic dissection of the dorsal side of the tumor and ligation of the feeding vessels, total resection of the primary tumor, extended posterior sectional hepatectomy, and partial vena cava resection were performed by open surgery via a thoracoabdominal approach. Abundant congestive bleeding with instability of vital signs occurred during transection. It could be finally controlled by dissect the remnant feeding artery in the inmost space. Prior control of arterial in-flow enabled successful completion of the planned surgical procedure. The patient has now survived for 27 months since resection of the primary lesion. Conclusion: Ligation of the feeding arteries to this hypervascular catecholamine-releasing tumor via a retroperitoneal laparoscopic approach prior to performing combined organ resection facilitated successful excision of this large malignant pheochromocytoma.
Language	English
Publishing date	2021-03-13
Publishing country	Netherlands
Document type	Case Reports
ISSN	2210-2612
ISSN	2210-2612
DOI	10.1016/j.ijscr.2021.105763
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Cutaneous metastasis emerging during chemotherapy and progressing during immunotherapy for urothelial carcinoma.

Otsuka, Isamu / Ueno, Takashi / Terada, Naoki / Mukai, Shoichiro / Fukushima, Tsuyoshi / Kiwaki, Takumi / Nagano, Masafumi

IJU case reports

2021 Volume 4, Issue 6, Page(s) 363–366

Abstract: Introduction: Cutaneous metastasis of urothelial carcinoma is uncommon. We experienced a case of cutaneous metastasis that emerged during chemotherapy and progressed rapidly during immunotherapy for bladder tumor with lymph node metastasis.: Case ... ...

Abstract	Introduction: Cutaneous metastasis of urothelial carcinoma is uncommon. We experienced a case of cutaneous metastasis that emerged during chemotherapy and progressed rapidly during immunotherapy for bladder tumor with lymph node metastasis. Case presentation: A 77-year-old female patient received chemotherapy using gemcitabine and cisplatin for bladder urothelial carcinoma with para-aortic and pelvic lymph node metastases (clinical stage T2N1M1). After eight courses of chemotherapy, skin rash appeared in the lower abdomen with leg edema caused by multiple lymphadenopathy. Skin biopsy revealed cutaneous metastasis of urothelial carcinoma. The cutaneous tumor progressed rapidly and the patient died 5 weeks after the start of second-line pembrolizumab treatment. Conclusion: A patient with cutaneous metastasis of urothelial carcinoma that emerged during chemotherapy had poor prognosis and lack of efficacy of an immune checkpoint inhibitor.
Language	English
Publishing date	2021-07-18
Publishing country	Australia
Document type	Case Reports
ISSN	2577-171X
ISSN (online)	2577-171X
DOI	10.1002/iju5.12350
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Insufficiency of hepatocyte growth factor activator inhibitor-1 confers lymphatic invasion of tongue carcinoma cells.

Izumi, Aya / Yamamoto, Koji / Kawaguchi, Makiko / Yamashita, Fumiki / Fukushima, Tsuyoshi / Kiwaki, Takumi / Tanaka, Hiroyuki / Yamashita, Yoshihiro / Kataoka, Hiroaki

Cancer science

2022 Volume 113, Issue 6, Page(s) 2179–2193

Abstract: Hepatocyte growth factor (HGF) activator inhibitor type-1 (HAI-1), encoded by the SPINT1 gene, is a transmembrane protease inhibitor that regulates membrane-anchored serine proteases, particularly matriptase. Here, we explored the role of HAI-1 in tongue ...

Abstract	Hepatocyte growth factor (HGF) activator inhibitor type-1 (HAI-1), encoded by the SPINT1 gene, is a transmembrane protease inhibitor that regulates membrane-anchored serine proteases, particularly matriptase. Here, we explored the role of HAI-1 in tongue squamous cell carcinoma (TSCC) cells. An immunohistochemical study of HAI-1 in surgically resected TSCC revealed the cell surface immunoreactivity of HAI-1 in the main portion of the tumor. The immunoreactivity decreased in the infiltrative front, and this decrease correlated with enhanced lymphatic invasion as judged by podoplanin immunostaining. In vitro homozygous deletion of SPINT1 (HAI-1KO) in TSCC cell lines (HSC3 and SAS) suppressed the cell growth rate but significantly enhanced invasion in vitro. The loss of HAI-1 resulted in enhanced pericellular activities of proteases, such as matriptase and urokinase-type plasminogen activator, which induced activation of HGF/MET signaling in the co-culture with pro-HGF-expressing fibroblasts and plasminogen-dependent plasmin generation, respectively. The enhanced plasminogen-dependent plasmin generation was abrogated partly by matriptase silencing. Culture supernatants of HAI-1KO cells had enhanced potency for converting the proform of vascular endothelial growth factor-C (VEGF-C), a lymphangiogenesis factor, into the mature form in a plasminogen-dependent manner. Furthermore, HGF significantly stimulated VEGF-C expression in TSCC cells. Orthotopic xenotransplantation into nude mouse tongue revealed enhanced lymphatic invasion of HAI-1KO TSCC cells compared to control cells. Our results suggest that HAI-1 insufficiency leads to dysregulated pericellular protease activity, which eventually orchestrates robust activation of protease-dependent growth factors, such as HGF and VEGF-C, in a tumor microenvironment to contribute to TSCC progression.
MeSH term(s)	Animals ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/pathology ; Fibrinolysin/genetics ; Homozygote ; Humans ; Mice ; Plasminogen/genetics ; Proteinase Inhibitory Proteins, Secretory/genetics ; Proteinase Inhibitory Proteins, Secretory/metabolism ; Sequence Deletion ; Serine Endopeptidases ; Tongue Neoplasms/genetics ; Tongue Neoplasms/pathology ; Tumor Microenvironment ; Vascular Endothelial Growth Factor C/genetics
Chemical Substances	Proteinase Inhibitory Proteins, Secretory ; SPINT1 protein, human ; Vascular Endothelial Growth Factor C ; Plasminogen (9001-91-6) ; Serine Endopeptidases (EC 3.4.21.-) ; Fibrinolysin (EC 3.4.21.7)
Language	English
Publishing date	2022-04-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2115647-5
ISSN	1349-7006 ; 1349-7006
ISSN (online)	1349-7006
ISSN	1349-7006
DOI	10.1111/cas.15346
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Role of the polycystic kidney disease domain in matriptase chaperone activity and localization of hepatocyte growth factor activator inhibitor‐1

Yamashita, Fumiki / Kaieda, Takashi / Shimomura, Takeshi / Kawaguchi, Makiko / Lin, Chen‐Yong / Johnson, Michael D. / Tanaka, Hiroyuki / Kiwaki, Takumi / Fukushima, Tsuyoshi / Kataoka, Hiroaki

FEBS journal. 2022 June, v. 289, no. 12 p.3422-3439

2022

Abstract: Hepatocyte growth factor activator inhibitor‐1 (HAI‐1, also known as SPINT1) is an inhibitor of matriptase, a type‐2 transmembrane protease widely expressed in epithelial cells. HAI‐1 also functions as a chaperone to maintain the processing and ... ...

Abstract	Hepatocyte growth factor activator inhibitor‐1 (HAI‐1, also known as SPINT1) is an inhibitor of matriptase, a type‐2 transmembrane protease widely expressed in epithelial cells. HAI‐1 also functions as a chaperone to maintain the processing and localization of matriptase required for epithelial integrity. However, mechanisms underpinning the chaperone function remain to be elucidated. Here, we show that the first Kunitz domain (KD1) and the adjacent polycystic kidney disease (PKD) domain‐like internal domain of HAI‐1 are essential for the chaperone function. In HEK293T cells, which do not express endogenous HAI‐1 or matriptase, forced matriptase overexpression was unsuccessful unless sufficient HAI‐1 was co‐expressed. Among mutant HAI‐1 constructs, HAI‐1 with inactivation mutation in KD1 (HAI‐1mKD1) or HAI‐1 lacking the PKD domain (HAI‐1dPKD) was unable to support matriptase expression, and neither mutant formed a complex with activated matriptase. Matriptase did not localize to the cell surface when co‐expressed with HAI‐1dPKD. Moreover, HAI‐1dPKD accumulated in the cytoplasm of HEK293T and HaCaT cells rather than localizing to the cell surface, presumably due to misfolding as judged by altered antibody recognition. On the other hand, activationlocked and activity‐incompetent matriptase were stable and readily overexpressed and localized to the cell surface without HAI‐1. Therefore, the observed matriptase instability was caused by its own catalytic activity in the absence of inhibitory HAI‐1. The matriptase chaperone function of HAI‐1 is thus mediated primarily by the inhibition of undesired intracellular matriptase activity, and the PKD domain is essential for the proper folding and trafficking of inhibitory HAI‐1 and its chaperone function.
Keywords	antibodies ; catalytic activity ; cytoplasm ; epithelium ; hepatocyte growth factor ; mutants ; mutation ; polycystic kidney diseases ; proteinases
Language	English
Dates of publication	2022-06
Size	p. 3422-3439.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	REVIEW
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/febs.16348
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 2006/704: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED