LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Back to previous search Search history
Advanced search

Your last searches

  1. AU="Klomkliew, Pavit"
  2. AU="Kitra, V"
  3. AU="Madaan, Nirupam"
  4. AU=Huang Xiaojia
  5. AU="Halder, Dibakar"
  6. AU="Leduc, Charles A"
  7. AU="Yoo, Jiwoon"
  8. AU="Davis, Sangeetha P"
  9. AU="Groesdonk, H."
  10. AU=Calorini Lido AU=Calorini Lido
  11. AU="Marina Umansky Sommer"
  12. AU="Al-Yousofy, Fayed"
  13. AU="Martínez-Peláez, Rafael"
  14. AU="Joumaa, Ranim"
  15. AU="Miranda, Alejandro"
  16. AU="Asselin, Yanick"
  17. AU="Napierala, Eric"
  18. AU="Hanna, S R"
  19. AU=Klukova Ludmila
  20. AU="Androutsakos, Theodoros"
  21. AU="Vrzal, Radim"
  22. AU="Carlo Gambacorti-Passerini"
  23. AU="Mahjabeen, Faria"
  24. AU="Roberto Giovannoni"
  25. AU="Rajendra Damu Patil"
  26. AU="Santos-García, Irene"
  27. AU="Josiah Willock, Robina"
  28. AU="Ciacci, Joseph"
  29. AU="Barker, A"
  30. AU="Chris Baraloto"
  31. AU="Wagner, Lloyd"
  32. AU="Jandrić, Petar Hayes"

Search results

Result 1 - 8 of total 8

Search options

  1. Article ; Online: "Nano COVID-19": Nanopore sequencing of spike gene to identify SARS-CoV-2 variants of concern.

    Nimsamer, Pattaraporn / Sawaswong, Vorthon / Klomkliew, Pavit / Kaewsapsak, Pornchai / Puenpa, Jiratchaya / Poovorawan, Yong / Payungporn, Sunchai

    Experimental biology and medicine (Maywood, N.J.)

    2023  Volume 248, Issue 20, Page(s) 1841–1849

    Abstract: Coronavirus disease 2019 (COVID-19) is a worldwide pandemic infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). World Health Organization (WHO) has defined the viral variants of concern (VOC) which cause more severe ...

    Abstract Coronavirus disease 2019 (COVID-19) is a worldwide pandemic infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). World Health Organization (WHO) has defined the viral variants of concern (VOC) which cause more severe disease, higher transmissibility, and reduced vaccine efficacy. In this study, the "Nano COVID-19" workflow based on Oxford nanopore sequencing of the full-length spike gene combined with flexible data analysis options was developed to identify SARS-CoV-2 VOCs. The primers were designed to cover the full-length spike gene and can amplify all VOC strains. The results of VOC identification based on phylogenetic analysis of the full-length spike gene were comparable to the whole genome sequencing (WGS). Compared to the standard VOC identification pipeline, the fast analysis based on Read Assignment, Mapping, and Phylogenetic Analysis in Real Time (RAMPART) and the user-friendly method based on EPI2ME yielded 89.3% and 97.3% accuracy, respectively. The EPI2ME pipeline is recommended for researchers without bioinformatic skills, whereas RAMPART is more suitable for bioinformaticians. This workflow provides a cost-effective, simplified pipeline with a rapid turnaround time. Furthermore, it is portable to point-of-care SARS-CoV-2 VOC identification and compatible with large-scale analysis. Therefore, "Nano COVID-19" is an alternative viral epidemic screening and transmission tracking workflow.
    MeSH term(s) Humans ; SARS-CoV-2/genetics ; COVID-19/diagnosis ; Nanopore Sequencing ; Phylogeny ; Mutation
    Language English
    Publishing date 2023-09-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 4015-0
    ISSN 1535-3699 ; 1525-1373 ; 0037-9727
    ISSN (online) 1535-3699 ; 1525-1373
    ISSN 0037-9727
    DOI 10.1177/15353702231190931
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.111: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Exploration of the Gut Microbiome in Thai Patients with Major Depressive Disorder Shows a Specific Bacterial Profile with Depletion of the

    Maes, Michael / Vasupanrajit, Asara / Jirakran, Ketsupar / Klomkliew, Pavit / Chanchaem, Prangwalai / Tunvirachaisakul, Chavit / Payungporn, Sunchai

    Cells

    2023  Volume 12, Issue 9

    Abstract: Maes et al. (2008) published the first paper demonstrating that major depressive disorder (MDD) is accompanied by abnormalities in the microbiota-gut-brain axis, as evidenced by elevated serum IgM/IgA to lipopolysaccharides (LPS) of Gram-negative ... ...

    Abstract Maes et al. (2008) published the first paper demonstrating that major depressive disorder (MDD) is accompanied by abnormalities in the microbiota-gut-brain axis, as evidenced by elevated serum IgM/IgA to lipopolysaccharides (LPS) of Gram-negative bacteria, such as
    MeSH term(s) Humans ; Depressive Disorder, Major ; Gastrointestinal Microbiome/genetics ; Ruminococcus ; Lipopolysaccharides/metabolism ; Southeast Asian People ; Biomarkers
    Chemical Substances Lipopolysaccharides ; Biomarkers
    Language English
    Publishing date 2023-04-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12091240
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Comparative analysis of gut microbiota between common (Macaca fascicularis fascicularis) and Burmese (M. f. aurea) long-tailed macaques in different habitats.

    Muhammad, Raza / Klomkliew, Pavit / Chanchaem, Prangwalai / Sawaswong, Vorthon / Kaikaew, Titiporn / Payungporn, Sunchai / Malaivijitnond, Suchinda

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 14950

    Abstract: The environment has an important effect on the gut microbiota-an essential part of the host's health-and is strongly influenced by the dietary pattern of the host as these together shape the composition and functionality of the gut microbiota in humans ... ...

    Abstract The environment has an important effect on the gut microbiota-an essential part of the host's health-and is strongly influenced by the dietary pattern of the host as these together shape the composition and functionality of the gut microbiota in humans and other animals. This study compared the gut microbiota of Macaca fascicularis fascicularis and M. f. aurea in mangrove and island populations using 16S rRNA gene sequencing on a nanopore platform to investigate the effect of the environment and/or diet. The results revealed that the M. f. fascicularis populations that received anthropogenic food exhibited a higher richness and evenness of gut microbiota than the M. f. aurea populations in different habitats. Firmicutes and Bacteroidetes were the two most abundant bacterial phyla in the gut microbiota of both these subspecies; however, the relative abundance of these phyla was significantly higher in M. f. aurea than in M. f. fascicularis. This variation in the gut microbiota between the two subspecies in different habitats mostly resulted from the differences in their diets. Moreover, the specific adaptation of M. f. aurea to different environments with a different food availability had a significant effect on their microbial composition.
    MeSH term(s) Animals ; Diet ; Ecosystem ; Gastrointestinal Microbiome/genetics ; Macaca fascicularis/genetics ; Macaca fascicularis/microbiology ; RNA, Ribosomal, 16S/genetics ; Species Specificity
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2023-09-11
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-42220-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: In vitro

    Khamwut, Ariya / Klomkliew, Pavit / Jumpathong, Watthanachai / Kaewsapsak, Pornchai / Chanchaem, Prangwalai / Sivapornnukul, Pavaret / Chantanakat, Kridsana / T-Thienprasert, Nattanan Panjaworayan / Payungporn, Sunchai

    Biomedical reports

    2023  Volume 19, Issue 4, Page(s) 70

    Abstract: Breast cancer is a leading cause of cancer-related deaths worldwide. Moreover, standard treatments are limited, so new alternative treatments are required. Thai traditional formulary medicine (TTFM) utilizes certain herbs to treat different diseases due ... ...

    Abstract Breast cancer is a leading cause of cancer-related deaths worldwide. Moreover, standard treatments are limited, so new alternative treatments are required. Thai traditional formulary medicine (TTFM) utilizes certain herbs to treat different diseases due to their dominant properties including anti-fungal, anti-bacterial, antigenotoxic, anti-inflammatory and anti-cancer actions. However, very little is known about the anti-cancer properties of TTFM against breast cancer cells and the underlying molecular mechanism has not been elucidated. Therefore, the present study, evaluated the metabolite profiles of TTFM extracts, the anti-cancer activities of TTFM extracts, their effects on the apoptosis pathway and associated gene expression profiles. Liquid chromatography with tandem mass spectroscopy analysis identified a total of 226 compounds within the TTFM extracts. Several of these compounds have been previously shown to have an anti-cancer effect in certain cancer types. The MTT results demonstrated that the TTFM extracts significantly reduced the cell viability of the breast cancer 4T1 and MDA-MB-231 cell lines. Moreover, an apoptosis assay, demonstrated that the TTFM extracts significantly increased the proportion of apoptotic cells. Furthermore, the RNA-sequencing results demonstrated that 25 known genes were affected by TTFM treatment in 4T1 cells. TTFM treatment significantly up-regulated
    Language English
    Publishing date 2023-08-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2763624-0
    ISSN 2049-9442 ; 2049-9434
    ISSN (online) 2049-9442
    ISSN 2049-9434
    DOI 10.3892/br.2023.1652
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Tissue and Plasma-Based Highly Sensitive Blocker Displacement Amplicon Nanopore Sequencing for EGFR Mutations in Lung Cancer.

    Akkhasutthikun, Patinya / Kaewsapsak, Pornchai / Nimsamer, Pattaraporn / Klomkliew, Pavit / Visedthorn, Suthida / Chanchaem, Pragwalai / Teerapakpinyo, Chinachote / Payungporn, Sunchai / Luangdilok, Sutima

    Cancer research and treatment

    2023  Volume 56, Issue 2, Page(s) 455–463

    Abstract: Purpose: The epidermal growth factor receptor (EGFR) mutation is a widely prevalent oncogene driver in non-small cell lung cancer (NSCLC) in East Asia. The detection of EGFR mutations is a standard biomarker test performed routinely in patients with ... ...

    Abstract Purpose: The epidermal growth factor receptor (EGFR) mutation is a widely prevalent oncogene driver in non-small cell lung cancer (NSCLC) in East Asia. The detection of EGFR mutations is a standard biomarker test performed routinely in patients with NSCLC for the selection of targeted therapy. Here, our objective was to develop a portable new technique for detecting EGFR (19Del, T790M, and L858R) mutations based on Nanopore sequencing.
    Materials and methods: The assay employed a blocker displacement amplification (BDA)-based polymerase chain reaction (PCR) technique combined with Nanopore sequencing to detect EGFR mutations. Mutant and wild-type EGFR clones were generated from DNA from H1650 (19Del heterozygous) and H1975 (T790M and L858R heterozygous) lung cancer cell lines. Then, they were mixed to assess the performance of this technique for detecting low variant allele frequencies (VAFs). Subsequently, formalin-fixed, paraffin-embedded (FFPE) tissue and cell-free DNA (cfDNA) from patients with NSCLC were used for clinical validation.
    Results: The assay can detect low VAF at 0.5% mutant mixed in wild-type EGFR. Using FFPE DNA, the concordance rates of EGFR 19Del, T790M, and L858R mutations between our method and Cobas real-time PCR were 98.46%, 100%, and 100%, respectively. For cfDNA, the concordance rates of EGFR 19Del, T790M, and L858R mutations between our method and droplet digital PCR were 94.74%, 100%, and 100%, respectively.
    Conclusion: The BDA amplicon Nanopore sequencing is a highly accurate and sensitive method for the detection of EGFR mutations in clinical specimens.
    MeSH term(s) Humans ; Carcinoma, Non-Small-Cell Lung/diagnosis ; Carcinoma, Non-Small-Cell Lung/genetics ; Cell-Free Nucleic Acids/genetics ; DNA, Neoplasm ; ErbB Receptors/genetics ; Lung Neoplasms/diagnosis ; Lung Neoplasms/genetics ; Mutation ; Nanopore Sequencing/methods ; Protein Kinase Inhibitors/therapeutic use ; Real-Time Polymerase Chain Reaction
    Chemical Substances Cell-Free Nucleic Acids ; DNA, Neoplasm ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1) ; Protein Kinase Inhibitors
    Language English
    Publishing date 2023-11-20
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2133613-1
    ISSN 2005-9256 ; 1598-2998
    ISSN (online) 2005-9256
    ISSN 1598-2998
    DOI 10.4143/crt.2023.1108
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 7043: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Adverse childhood experiences and reoccurrence of illness impact the gut microbiome, which affects suicidal behaviours and the phenome of major depression: towards enterotypic phenotypes.

    Maes, Michael / Vasupanrajit, Asara / Jirakran, Ketsupar / Klomkliew, Pavit / Chanchaem, Prangwalai / Tunvirachaisakul, Chavit / Plaimas, Kitiporn / Suratanee, Apichat / Payungporn, Sunchai

    Acta neuropsychiatrica

    2023  Volume 35, Issue 6, Page(s) 328–345

    Abstract: The first publication demonstrating that major depressive disorder (MDD) is associated with alterations in the gut microbiota appeared in 2008 ( ... ...

    Abstract The first publication demonstrating that major depressive disorder (MDD) is associated with alterations in the gut microbiota appeared in 2008 (Maes
    MeSH term(s) Humans ; Depressive Disorder, Major/genetics ; Gastrointestinal Microbiome/genetics ; Depression ; Feces/microbiology ; Adverse Childhood Experiences ; Suicidal Ideation ; Phenotype
    Language English
    Publishing date 2023-04-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 1154361-9
    ISSN 1601-5215 ; 0924-2708
    ISSN (online) 1601-5215
    ISSN 0924-2708
    DOI 10.1017/neu.2023.21
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3708: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques.

    Sawaswong, Vorthon / Chanchaem, Prangwalai / Klomkliew, Pavit / Rotcheewaphan, Suwatchareeporn / Meesawat, Suthirote / Kemthong, Taratorn / Kaewparuehaschai, Mutchamon / Noradechanon, Kirana / Ekatat, Monya / Kanitpun, Reka / Srilohasin, Prapaporn / Warit, Saradee / Chaiprasert, Angkana / Malaivijitnond, Suchinda / Payungporn, Sunchai

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3404

    Abstract: Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study ... ...

    Abstract Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis, but the study was limited. The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB (-) control, TB (+) latent and TB (+) active groups. The differential abundance analysis showed that Haemophilus hemolyticus was decreased, while Prevotella species were increased in the pharyngeal microbiome of TB (+) macaques. In addition, Eubacterium coprostanoligenes in the gut was enriched in TB (+) macaques. Alteration of these bacteria might affect immune regulation and TB severity, but details of mechanisms should be further explored and validated. In summary, microbiota may be associated with host immune regulation and affect TB progression. The findings suggested the potential mechanisms of host-microbes interaction, which may improve the understanding of the role of microbiota and help develop therapeutics for TB in the future.
    MeSH term(s) Animals ; Nanopores ; Tuberculosis/microbiology ; Gastrointestinal Microbiome/genetics ; Microbiota/genetics ; Macaca fascicularis/genetics ; RNA, Ribosomal, 16S/genetics
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-02-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-53880-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Gut bacteriome and metabolome of Ascaris lumbricoides in patients.

    Klomkliew, Pavit / Sawaswong, Vorthon / Chanchaem, Prangwalai / Nimsamer, Pattaraporn / Adisakwattana, Poom / Phuphisut, Orawan / Tipthara, Phornpimon / Tarning, Joel / Payungporn, Sunchai / Reamtong, Onrapak

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 19524

    Abstract: The most frequent intestinal helminth infections in humans are attributed to Ascaris lumbricoides, and there are concerns over the anthelminthic resistance of this species. The gut microbiota has essential roles in host physiology. Therefore, discovering ...

    Abstract The most frequent intestinal helminth infections in humans are attributed to Ascaris lumbricoides, and there are concerns over the anthelminthic resistance of this species. The gut microbiota has essential roles in host physiology. Therefore, discovering host-parasite-microbiota interactions could help develop alternative helminthiasis treatments. Additionally, these interactions are modulated by functional metabolites that can reveal the mechanisms of infection and disease progression. Thus, we aimed to investigate bacteriomes in the gut of helminths and fecal samples of patients via next-generation sequencing. Our results showed that infection intensity was associated with the bacterial composition of helminth guts but not with the intestinal bacteriome of human hosts. Moreover, the metabolomes of A. lumbricoides in the heavy and light ascariasis cases were characterized using ultra-high performance liquid chromatography/time-of-flight mass spectrometry. Increased levels of essential biomolecules, such as amino acids, lipids, and nucleotide precursors, were found in the guts of helminths isolated from heavily infected patients, implying that these metabolites are related to egg production and ascariasis pathogenicity. These findings are the first step towards a more complete understanding of the mechanisms by which the bacteriome of helminth guts affect their colonization and may reveal novel and more effective approaches to parasitic disease therapy.
    MeSH term(s) Humans ; Animals ; Ascaris lumbricoides ; Ascariasis ; Gastrointestinal Microbiome ; Helminthiasis/parasitology ; Helminths ; Feces/parasitology ; Metabolome
    Language English
    Publishing date 2022-11-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-23608-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top