LIVIVO - Suchergebnisse -

Suchergebnis

Treffer 1 - 10 von insgesamt 17

Suchoptionen

Dissertation / Habilitation ; Online: The potential of tailor-made resistant starch type 3 as a health-beneficial ingredient for the gut

Klostermann, Cynthia E.

2023

Schlagwörter	Life Science
Sprache	Englisch
Verlag	Wageningen University
Erscheinungsland	nl
Dokumenttyp	Dissertation / Habilitation ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Artikel ; Online: The action of endo-xylanase and endo-glucanase on cereal cell wall polysaccharides and its implications for starch digestion kinetics in an in vitro poultry model.

Kouzounis, Dimitrios / Nguyen, Khoa A / Klostermann, Cynthia E / Soares, Natalia / Kabel, Mirjam A / Schols, Henk A

Carbohydrate polymers

2024 Band 331, Seite(n) 121861

Abstract: Endo-xylanase and endo-glucanase are supplemented to poultry diets in order to improve nutrient digestion and non-starch polysaccharide (NSP) fermentation. Here, the action of these enzymes on alcohol insoluble solids (AIS) from wheat and maize grains as ...

Abstract	Endo-xylanase and endo-glucanase are supplemented to poultry diets in order to improve nutrient digestion and non-starch polysaccharide (NSP) fermentation. Here, the action of these enzymes on alcohol insoluble solids (AIS) from wheat and maize grains as well as its implications for starch digestion in milled grains were evaluated in vitro, under conditions mimicking the poultry digestive tract. For wheat AIS, GH11 endo-xylanase depolymerized soluble arabinoxylan (AX) during the gizzard phase, and proceeded to release insoluble AX under small intestine conditions. At the end of the in vitro digestion (480 min), the endo-xylanase, combined with a GH7 endo-β-1,4-glucanase, released 30.5 % of total AX and 18.1 % of total glucan in the form of arabinoxylo- and gluco-oligosaccharides, as detected by HPAEC-PAD and MALDI-TOF-MS. For maize AIS, the combined enzyme action released 2.2 % and 7.0 % of total AX and glucan, respectively. Analogous in vitro digestion experiments of whole grains demonstrated that the enzymatic release of oligomers coincided with altered grain microstructure, as examined by SEM. In the present study, cell wall hydrolysis did not affect in vitro starch digestion kinetics for cereal grains. This study contributes to understanding the action of feed enzymes on cereal NSP under conditions mimicking the poultry digestive tract.
Mesh-Begriff(e)	Animals ; Starch/analysis ; Edible Grain/chemistry ; Poultry ; Polysaccharides/analysis ; Diet ; Glucans/analysis ; Digestion ; Cell Wall ; Animal Feed/analysis ; Endo-1,4-beta Xylanases
Chemische Substanzen	Starch (9005-25-8) ; Polysaccharides ; Glucans ; Endo-1,4-beta Xylanases (EC 3.2.1.8)
Sprache	Englisch
Erscheinungsdatum	2024-01-27
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2024.121861
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Über subito bestellen

Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Details ▾
- Volltext online
- Kostenpflichtig bestellen

Artikel ; Online: Presence of digestible starch impacts

Klostermann, Cynthia E / Endika, Martha F / Kouzounis, Dimitrios / Buwalda, Piet L / de Vos, Paul / Zoetendal, Erwin G / Bitter, Johannes H / Schols, Henk A

Food & function

2024 Band 15, Heft 1, Seite(n) 223–235

Abstract: Starch is an important energy source for humans. Starch escaping digestion in the small intestine will transit to the colon to be fermented by gut microbes. Many gut microbes express α-amylases that can degrade soluble starch, but only a few are able to ... ...

Abstract	Starch is an important energy source for humans. Starch escaping digestion in the small intestine will transit to the colon to be fermented by gut microbes. Many gut microbes express α-amylases that can degrade soluble starch, but only a few are able to degrade intrinsic resistant starch (RS), which is insoluble and highly resistant to digestion (≥80% RS). We studied the
Mesh-Begriff(e)	Infant ; Adult ; Humans ; Resistant Starch/metabolism ; Fermentation ; Starch/metabolism ; Feces/microbiology ; Acetates ; Digestion
Chemische Substanzen	Resistant Starch ; Starch (9005-25-8) ; Acetates
Sprache	Englisch
Erscheinungsdatum	2024-01-02
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2612033-1
ISSN	2042-650X ; 2042-6496
ISSN (online)	2042-650X
ISSN	2042-6496
DOI	10.1039/d3fo01763j
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Verfügbar in ZB MED Bonn

Z 7872: Hefte anzeigen

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾
- Im ZB MED-Bestand
- Kostenpflichtig bestellen

Artikel ; Online: The action of endo-xylanase and endo-glucanase on cereal cell wall polysaccharides and its implications for starch digestion kinetics in an in vitro poultry model

Kouzounis, Dimitrios / Nguyen, Khoa A. / Klostermann, Cynthia E. / Soares, Natalia / Kabel, Mirjam A. / Schols, Henk A.

Carbohydrate Polymers

2024 Band 331

Abstract	Endo-xylanase and endo-glucanase are supplemented to poultry diets in order to improve nutrient digestion and non-starch polysaccharide (NSP) fermentation. Here, the action of these enzymes on alcohol insoluble solids (AIS) from wheat and maize grains as well as its implications for starch digestion in milled grains were evaluated in vitro, under conditions mimicking the poultry digestive tract. For wheat AIS, GH11 endo-xylanase depolymerized soluble arabinoxylan (AX) during the gizzard phase, and proceeded to release insoluble AX under small intestine conditions. At the end of the in vitro digestion (480 min), the endo-xylanase, combined with a GH7 endo-β-1,4-glucanase, released 30.5 % of total AX and 18.1 % of total glucan in the form of arabinoxylo- and gluco-oligosaccharides, as detected by HPAEC-PAD and MALDI-TOF-MS. For maize AIS, the combined enzyme action released 2.2 % and 7.0 % of total AX and glucan, respectively. Analogous in vitro digestion experiments of whole grains demonstrated that the enzymatic release of oligomers coincided with altered grain microstructure, as examined by SEM. In the present study, cell wall hydrolysis did not affect in vitro starch digestion kinetics for cereal grains. This study contributes to understanding the action of feed enzymes on cereal NSP under conditions mimicking the poultry digestive tract.
Schlagwörter	Endo-glucanase ; Endo-xylanase ; Feed enzymes ; In vitro digestion ; Prebiotic oligosaccharides ; Starch digestion kinetics
Thema/Rubrik (Code)	660
Sprache	Englisch
Erscheinungsland	nl
Dokumenttyp	Artikel ; Online
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Über subito bestellen

Details ▾
- Volltext online
- Kostenpflichtig bestellen

Artikel ; Online: Presence of digestible starch impacts in vitro fermentation of resistant starch

Klostermann, Cynthia E. / Endika, Martha F. / Kouzounis, Dimitrios / Buwalda, Piet L. / de Vos, Paul / Zoetendal, Erwin G. / Bitter, Johannes H. / Schols, Henk A.

Food and Function

2024 Band 15, Heft 1

Abstract	Starch is an important energy source for humans. Starch escaping digestion in the small intestine will transit to the colon to be fermented by gut microbes. Many gut microbes express α-amylases that can degrade soluble starch, but only a few are able to degrade intrinsic resistant starch (RS), which is insoluble and highly resistant to digestion (≥80% RS). We studied the in vitro fermentability of eight retrograded starches (RS-3 preparations) differing in rapidly digestible starch content (≥70%, 35-50%, ≤15%) by a pooled adult faecal inoculum and found that fermentability depends on the digestible starch fraction. Digestible starch was readily fermented yielding acetate and lactate, whereas resistant starch was fermented much slower generating acetate and butyrate. Primarily Bifidobacterium increased in relative abundance upon digestible starch fermentation, whereas resistant starch fermentation also increased relative abundance of Ruminococcus and Lachnospiraceae. The presence of small fractions of total digestible starch (±25%) within RS-3 preparations influenced the fermentation rate and microbiota composition, after which the resistant starch fraction was hardly fermented. By short-chain fatty acid quantification, we observed that six individual faecal inocula obtained from infants and adults were able to ferment digestible starch, whereas only one adult faecal inoculum was fermenting intrinsic RS-3. This suggests that, in contrast to digestible starch, intrinsic RS-3 is only fermentable when specific microbes are present. Our data illustrates that awareness is required for the presence of digestible starch during in vitro fermentation of resistant starch, since such digestible fraction might influence and overrule the evalution of the prebiotic potential of resistant starches.
Schlagwörter	Life Science
Thema/Rubrik (Code)	660
Sprache	Englisch
Erscheinungsland	nl
Dokumenttyp	Artikel ; Online
ZDB-ID	2612033-1
ISSN	2042-650X ; 2042-6496
ISSN (online)	2042-650X
ISSN	2042-6496
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Verfügbar in ZB MED Bonn

Z 7872: Hefte anzeigen

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾

Artikel: Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin

Verkhnyatskaya, Stella A / Kong, Chunli / Klostermann, Cynthia E / Schols, Henk A / de Vos, Paul / Walvoort, Marthe T. C

Food & function. 2021 June 8, v. 12, no. 11

2021

Abstract: Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features. Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs). Especially ... ...

Abstract	Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features. Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs). Especially fucosylated hMOs have received attention for their anti-adhesive effects on pathogens, preventing attachment to the intestine and infection. Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called “mimics”) are interesting compounds to produce and evaluate for their biological effects. Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs. We establish that DFβCD is not digested by α-amylase and also resistant to fermentation by microbial enzymes from a 9 month-old infant inoculum. In addition, we reveal that DFβCD blocks adhesion of enterotoxigenic E. coli (ETEC) to Caco-2 cells, especially when DFβCD is pre-incubated with ETEC prior to addition to the Caco-2 cells. This suggests that DFβCD functions through a decoy effect. We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.
Schlagwörter	adhesion ; alpha-amylase ; biological assessment ; breast milk ; digestion ; enterotoxigenic Escherichia coli ; fermentation ; infant formulas ; inoculum ; intestines ; oligosaccharides ; pathogens ; polysaccharides
Sprache	Englisch
Erscheinungsverlauf	2021-0608
Umfang	p. 5018-5026.
Erscheinungsort	The Royal Society of Chemistry
Dokumenttyp	Artikel
Anmerkung	NAL-AP-2-clean
ZDB-ID	2612033-1
ISSN	2042-650X ; 2042-6496
ISSN (online)	2042-650X
ISSN	2042-6496
DOI	10.1039/d1fo00830g
Datenquelle	NAL Katalog (AGRICOLA)

Zusatzmaterialien

Verfügbar in ZB MED Bonn

Z 7872: Hefte anzeigen

Über subito bestellen

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Details ▾
- Im ZB MED-Bestand
- Kostenpflichtig bestellen

Artikel ; Online: From

Duran, Katharina / Kohlstedt, Michael / van Erven, Gijs / Klostermann, Cynthia E / America, Antoine H P / Bakx, Edwin / Baars, Johan J P / Gorissen, Antonie / de Visser, Ries / de Vries, Ronald P / Wittmann, Christoph / Comans, Rob N J / Kuyper, Thomas W / Kabel, Mirjam A

Science advances

2024 Band 10, Heft 16, Seite(n) eadl3419

Abstract: Plant biomass conversion by saprotrophic fungi plays a pivotal role in terrestrial carbon (C) cycling. The general consensus is that fungi metabolize carbohydrates, while lignin is only degraded and mineralized to ... ...

Abstract	Plant biomass conversion by saprotrophic fungi plays a pivotal role in terrestrial carbon (C) cycling. The general consensus is that fungi metabolize carbohydrates, while lignin is only degraded and mineralized to CO
Mesh-Begriff(e)	Lignin/metabolism ; Carbon/metabolism ; Mycelium/metabolism ; Carbohydrates ; Amino Acids ; Agaricus
Chemische Substanzen	Lignin (9005-53-2) ; Carbon (7440-44-0) ; Carbohydrates ; Amino Acids
Sprache	Englisch
Erscheinungsdatum	2024-04-19
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	2810933-8
ISSN	2375-2548 ; 2375-2548
ISSN (online)	2375-2548
ISSN	2375-2548
DOI	10.1126/sciadv.adl3419
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Buch ; Online: RS-3 fermentation adult

Klostermann, Cynthia E. / Endika, Martha F. / Kouzounis, Dimitrios / Buwalda, Piet L. / de Vos, Paul / Zoetendal, Erwin G. / Bitter, Johannes H. / Schols, Henk A.

2023

Abstract: Twelve novel resistant starch type 3 substrates differing in crystal type (A/B), alpha-1,4 glucan chain length (degree of polymerization 14 - 76) and molecular weight distribution (1.08 - 2.11) were fermented in vitro using an inoculum of pooled faeces ... ...

Abstract	Twelve novel resistant starch type 3 substrates differing in crystal type (A/B), alpha-1,4 glucan chain length (degree of polymerization 14 - 76) and molecular weight distribution (1.08 - 2.11) were fermented in vitro using an inoculum of pooled faeces obtained from four healthy adults. A selection of substrates was also treated with pancreatin to remove an easily digestible fraction, prior to in vitro fermentation using the same pooled adult faecal inoculum. Twelve novel resistant starch type 3 substrates differing in crystal type (A/B), alpha-1,4 glucan chain length (degree of polymerization 14 - 76) and molecular weight distribution (1.08 - 2.11) were fermented in vitro using an inoculum of pooled faeces obtained from four healthy adults. A selection of substrates was also treated with pancreatin to remove an easily digestible fraction, prior to in vitro fermentation using the same pooled adult faecal inoculum.
Schlagwörter	starch type 3 fermentation
Verlag	Wageningen University & Research
Erscheinungsland	nl
Dokumenttyp	Buch ; Online
Datenquelle	BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

Volltext online

Volltext online

Zusatzmaterialien

Fernleihe an ZB MED

Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Artikel: Microbiota-dependent influence of prebiotics on the resilience of infant gut microbiota to amoxicillin/clavulanate perturbation in an

Endika, Martha F / Barnett, David J M / Klostermann, Cynthia E / Schols, Henk A / Arts, Ilja C W / Penders, John / Nauta, Arjen / Smidt, Hauke / Venema, Koen

Frontiers in microbiology

2023 Band 14, Seite(n) 1131953

Abstract: Antibiotic exposure disturbs the developing infant gut microbiota. The capacity of the gut microbiota to recover from this disturbance (resilience) depends on the type of antibiotic. In this study, infant gut microbiota was exposed to a combination of ... ...

Abstract	Antibiotic exposure disturbs the developing infant gut microbiota. The capacity of the gut microbiota to recover from this disturbance (resilience) depends on the type of antibiotic. In this study, infant gut microbiota was exposed to a combination of amoxicillin and clavulanate (amoxicillin/clavulanate) in an
Sprache	Englisch
Erscheinungsdatum	2023-05-18
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1131953
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Zusatzmaterialien

Über subito bestellen

Details ▾
- Kostenpflichtig bestellen

Artikel ; Online: Crystal type, chain length and polydispersity impact the resistant starch type 3 immunomodulatory capacity via Toll-like receptors.

Silva Lagos, Luis / Klostermann, Cynthia E / López-Velázquez, Gabriel / Fernández-Lainez, Cynthia / Leemhuis, Hans / Oudhuis, A A C M Lizette / Buwalda, Piet / Schols, Henk A / de Vos, Paul

Carbohydrate polymers

2023 Band 324, Seite(n) 121490

Abstract: Food ingredients that can activate and improve immunological defense, against e.g., pathogens, have become a major field of research. Resistant starches (RSs) can resist enzymes in the upper gastrointestinal (GI) tract and induce health benefits. RS-3 ... ...

Abstract	Food ingredients that can activate and improve immunological defense, against e.g., pathogens, have become a major field of research. Resistant starches (RSs) can resist enzymes in the upper gastrointestinal (GI) tract and induce health benefits. RS-3 physicochemical characteristics such as chain length (DP), A- or B-type crystal, and polydispersity index (PI) might be crucial for immunomodulation by activating human toll-like receptors (hTLRs). We hypothesize that crystal type, DP and PI, alone or in combination, impact the recognition of RS-3 preparations by hTLRs leading to different RS-3 immunomodulatory effects. We studied the activation of hTLR2, hTLR4, and hTLR5 by 0.5, 1 and 2 mg/mL of RS-3. We found strong activation of hTLR2-dependent NF-kB activation with PI <1.25, DP 18 as an A- or B-type crystal. At different doses, NF-kB activation was increased from 6.8 to 7.1 and 10-fold with A-type and 6.2 to 10.2 and 14.4-fold with B-type. This also resulted in higher cytokine production in monocytes. Molecular docking, using amylose-A and B, demonstrated that B-crystals bind hTLR2 promoting hTLR2-1 dimerization, supporting the stronger effects of B-type crystals. Immunomodulatory effects of RS-3 are predominantly hTLR2-dependent, and activation can be tailored by managing crystallinity, chain length, and PI.
Mesh-Begriff(e)	Humans ; Resistant Starch ; NF-kappa B/metabolism ; Molecular Docking Simulation ; Toll-Like Receptors ; Amylose/chemistry ; Starch/chemistry
Chemische Substanzen	Resistant Starch ; NF-kappa B ; Toll-Like Receptors ; Amylose (9005-82-7) ; Starch (9005-25-8)
Sprache	Englisch
Erscheinungsdatum	2023-10-13
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2023.121490
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

Volltext online

Zugriff für angemeldete ZB MED Nutzerinnen und Nutzer

Zusatzmaterialien

Über subito bestellen

Details ▾
- Volltext online
- Kostenpflichtig bestellen

Zum Seitenanfang

Ihre letzten Suchen

Volltext online

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Volltext online

Zusatzmaterialien

Kategorien

Über subito bestellen

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Bonn

Über subito bestellen

Fernleihe an ZB MED

Volltext online

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Bonn

Über subito bestellen

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Verfügbar in ZB MED Bonn

Über subito bestellen

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Fernleihe an ZB MED

Zusatzmaterialien

Kategorien

Über subito bestellen

Volltext online

Zusatzmaterialien

Kategorien

Über subito bestellen