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  1. Article ; Online: Experiences in reverse sequence esophagectomy: a promising alternative for esophageal cancer surgery.

    Lin, Chih-Hung / Chuang, Cheng-Yen / Ko, Jiunn-Liang / Hsu, Chung-Ping

    Surgical endoscopy

    2023  Volume 37, Issue 9, Page(s) 6749–6760

    Abstract: Objectives: McKeown esophagectomy is a standard and significant component of multimodality therapy in esophageal cancer, however, experience in switching the resection and reconstruction sequence in esophageal cancer surgery is not available. Here, we ... ...

    Abstract Objectives: McKeown esophagectomy is a standard and significant component of multimodality therapy in esophageal cancer, however, experience in switching the resection and reconstruction sequence in esophageal cancer surgery is not available. Here, we have retrospectively reviewed the experience of reverse sequencing procedure at our institute.
    Methods: We retrospectively reviewed 192 patients who had undergone minimally invasive esophagectomy (MIE) with McKeown esophagectomy between August 2008 and Dec 2015. The patient's demographics and relevant variables were evaluated. The overall survival (OS) and disease-free survival (DFS) were analyzed.
    Results: Among the 192 patients, 119 (61.98%) received the reverse sequence MIE (the reverse group) and 73 patients (38.02%) received the standard operation (the standard group). Both patient groups had similar demographics. There were no inter-group differences existed in blood loss, hospital stay, conversion rate, resection margin status, operative complication, and mortality. The reverse group had shorter total operation time (469.83 ± 75.03 vs 523.63 ± 71.93, p < 0.001) and thoracic operation time (181.22 ± 42.79 vs 230.41 ± 51.93, p < 0.001). The 5-year OS and DFS for both groups were similar (44.77% and 40.53% in the reverse group vs 32.66% and 29.42% in the standard group, p = 0.252 and 0.261, respectively). Similar results were observed even after propensity matching.
    Conclusions: The reverse sequence procedure had shorter operation times, especially in the thoracic phase. The reverse sequence MIE is a safe and useful procedure when postoperative morbidity, mortality, and oncological outcomes are considered.
    MeSH term(s) Humans ; Treatment Outcome ; Esophagectomy/methods ; Retrospective Studies ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Postoperative Complications/surgery ; Esophageal Neoplasms ; Minimally Invasive Surgical Procedures/methods
    Language English
    Publishing date 2023-05-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-023-10120-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Genistein protects against ultraviolet B-induced wrinkling and photoinflammation in in vitro and in vivo models.

    Tang, Sheau-Chung / Hsiao, Yu-Ping / Ko, Jiunn-Liang

    Genes & nutrition

    2022  Volume 17, Issue 1, Page(s) 4

    Abstract: Background: Chronic exposure to ultraviolet (UV) rays causes severe skin damage by inducing oxidative stress and inflammation. Identifying a safe and natural substance for skin protection is a crucial research goal.: Objective: The aim of this study ... ...

    Abstract Background: Chronic exposure to ultraviolet (UV) rays causes severe skin damage by inducing oxidative stress and inflammation. Identifying a safe and natural substance for skin protection is a crucial research goal.
    Objective: The aim of this study was to clarify the effects of genistein on skin inflammation and photoaging by using 3 models (humans: skin parameters; animals: wrinkle formation; and cells: anti-inflammatory effects).
    Methods: Food frequency questionnaire data and serum and skin parameter data from 120 volunteers (a group with a genistein-rich diet [RG group] and a control group). Human keratinocytes were pretreated with genistein before ultraviolet B (UVB) irradiation. Genistein was topically applied to the dorsal skin of rats.
    Results: The blood samples of the RG group had lower serum uric acid levels and blood urea nitrogen levels. The dynamic elasticity level in the RG group was higher than that in the controls. Genistein pretreatment suppressed the expression of proinflammatory cytokines (CXCL1, IL-1, MIF, and PLANH1) and the proteins released by UVB-treated keratinocytes. Topical application of genistein to the dorsal skin of rats reduced the severity of UVB-induced wrinkling. Both intake and topical application of genistein combated UVB-induced inflammation and aging.
    Conclusions: Genistein could be used as a safe and natural compound for use in novel anti-inflammatory agents for topical application. The experimental design procedure, including the skin parameter and blood serum measurements of 137 participants. Genistein-rich compounds provide protection against UVB-induced inflammation, as determined using in vitro and in vivo animal model experiments.
    Language English
    Publishing date 2022-02-24
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2416599-2
    ISSN 1865-3499 ; 1555-8932
    ISSN (online) 1865-3499
    ISSN 1555-8932
    DOI 10.1186/s12263-022-00706-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Pilot production of a sensitive ELISA kit and an immunochromatographic strip for rapid detecting citrinin in fermented rice.

    Wu, Shih-Wei / Ko, Jiunn-Liang / Liu, Biing-Hui / Yu, Feng-Yih

    RSC advances

    2022  Volume 12, Issue 31, Page(s) 19981–19989

    Abstract: Citrinin (CTN) is a mycotoxin primarily produced ... ...

    Abstract Citrinin (CTN) is a mycotoxin primarily produced by
    Language English
    Publishing date 2022-07-08
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d2ra02823a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Exposure to nickel chloride induces epigenetic modification on detoxification enzyme glutathione S-transferase M2.

    Kang, Yu-Ting / Yang, Wan-Jung / Huang, Hsu Chih / Tang, Sheau-Chung / Ko, Jiunn-Liang

    Environmental toxicology

    2023  Volume 39, Issue 3, Page(s) 1729–1736

    Abstract: Nickel (Ni) is a human carcinogen with genotoxic and epigenotoxic effects. Environmental and occupational exposure to Ni increases the risk of cancer and chronic inflammatory diseases. Our previous findings indicate that Ni alters gene expression through ...

    Abstract Nickel (Ni) is a human carcinogen with genotoxic and epigenotoxic effects. Environmental and occupational exposure to Ni increases the risk of cancer and chronic inflammatory diseases. Our previous findings indicate that Ni alters gene expression through epigenetic regulation, specifically impacting E-cadherin and angiopoietin-like 4 (ANGPTL4), involved in epithelial-mesenchymal transition and migration. GST-M2, a member of the glutathione S-transferase (GST) enzyme family, plays a crucial role in cellular defense against oxidative damage and has been increasingly associated with cancer. GST-M2 overexpression inhibits lung cancer invasion and metastasis in vitro and in vivo. Hypermethylation of its promoter in cancer cells reduces gene expression, correlating with poor prognosis in non-small-cell lung cancer patients. The impact of Ni on GST-M2 remains unclear. We will investigate whether nickel exerts regulatory effects on GST-M2 through epigenetic modifications. Additionally, metformin, an antidiabetic drug, is being studied as a chemopreventive agent against nickel-induced damage. Our findings indicate that nickel chloride (NiCl
    MeSH term(s) Humans ; Nickel ; Carcinoma, Non-Small-Cell Lung/genetics ; Epigenesis, Genetic ; Lung Neoplasms/pathology ; Glutathione Transferase/metabolism ; Metformin
    Chemical Substances nickel chloride (696BNE976J) ; Nickel (7OV03QG267) ; Glutathione Transferase (EC 2.5.1.18) ; Metformin (9100L32L2N)
    Language English
    Publishing date 2023-12-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1463449-1
    ISSN 1522-7278 ; 1520-4081
    ISSN (online) 1522-7278
    ISSN 1520-4081
    DOI 10.1002/tox.24055
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Multiplexed transcriptional profiling of Dermatophagoides house dust mites allergens in human epithelium cells.

    Lin, Chia-Yang / Cheng, Chun-Wen / Ko, Jiunn-Liang / Lue, Ko-Huang / Liu, Yu-Fan

    Environmental toxicology

    2023  Volume 39, Issue 4, Page(s) 2229–2239

    Abstract: Allergic asthma, a chronic disease characterized by airway inflammation, poses a significant public health concern. It is well-established that house dust mites (HDMs) are common inducers of allergic responses in individuals, particularly children. In ... ...

    Abstract Allergic asthma, a chronic disease characterized by airway inflammation, poses a significant public health concern. It is well-established that house dust mites (HDMs) are common inducers of allergic responses in individuals, particularly children. In central Taiwan, our research team observed that over 80% of allergic children exhibited sensitization to various HDMs species. This investigation aims to bridge the gap between these observations and a better understanding of the early fundamental mechanisms for preventing allergic diseases. Specifically, our study delves into the impact of crude extracts of HDMs on human epithelial BEAS-2B cells. Our findings, based on RNA sequencing (RNA-seq) analysis, shed light on how three major Dermatophagoides HDMs allergens activate a common Toll-like receptor signaling pathway in human epithelial cells within a 4-h treatment. During this process, the nuclear transcription factor NF-κB translocated into the cell nucleus within 30 min of allergen stimulation, triggering the expression of pro-inflammatory genes such as IL-6 and IL-8 over 4 h. Additionally, when the cells were treated with specific Dermatophagoides microceras (Der m) allergens, it resulted in the upregulation of genes that regulate type 1 diabetes mellitus (T1DM) signaling pathways. This led to the mediation of IL-12A inflammation. Furthermore, there was an increase in gene sets associated with cilia function and the microtubule cytoskeleton in human epithelial cells after treatment with a combination of Der m allergens and Dexamethasone. Additionally, OMICs analysis was conducted to examine the effects of HDMs allergenic stimulation on human epidermal cells. We aimed to improve our understanding of the molecular mechanisms within cells and identify potential targets and natural products in the treatment of asthma caused by HDMs allergens.
    MeSH term(s) Child ; Animals ; Humans ; Hypersensitivity ; Allergens/analysis ; Asthma/genetics ; Pyroglyphidae ; Epithelium/chemistry ; Inflammation ; Dust
    Chemical Substances Allergens ; Dust
    Language English
    Publishing date 2023-12-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1463449-1
    ISSN 1522-7278 ; 1520-4081
    ISSN (online) 1522-7278
    ISSN 1520-4081
    DOI 10.1002/tox.24109
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Pilot production of a sensitive ELISA kit and an immunochromatographic strip for rapid detecting citrinin in fermented rice

    Wu, Shih-Wei / Ko, Jiunn-Liang / Liu, Biing-Hui / Yu, Feng-Yih

    RSC advances. 2022 July 08, v. 12, no. 31

    2022  

    Abstract: Citrinin (CTN) is a mycotoxin primarily produced by Monascus species. Excess consumption of CTN may lead to nephrotoxicity and hepatotoxicity. A pilot study for commercial production of competitive direct enzyme-linked immunosorbent assay (cdELISA) kit ... ...

    Abstract Citrinin (CTN) is a mycotoxin primarily produced by Monascus species. Excess consumption of CTN may lead to nephrotoxicity and hepatotoxicity. A pilot study for commercial production of competitive direct enzyme-linked immunosorbent assay (cdELISA) kit and an immunochromatographic strip (immunostrip) for screening CTN in red yeast rice is established in this study. The coating antibody and the CTN–horse radish peroxidase (HRP) concentrations were optimized to increase the sensitivity and specificity of cdELISA kit. The conjugation methods/ratios of CTN to HRP as well as the long-term stability of kit components were also evaluated. The IC₅₀ and detection limit of the ELISA kit were determined to be 4.1 and 0.2 ng mL⁻¹, respectively. Analysis of 20 red yeast rice samples using ELISA kits revealed the contamination levels of CTN from 64 to 29 404 ng g⁻¹. The on-site rapid detection of CTN with the immunostrip showed that CTN levels in seven samples exceeded the regulatory limit of 5 ppm. Additionally, the coefficient correlation between the results of HPLC and ELISA kits of 20 samples was 0.96. Sensitive and convenient tools at commercial levels for detection of CTN contamination in food are established herein to protect the health of the public.
    Keywords Monascus ; antibodies ; citrinin ; detection limit ; enzyme-linked immunosorbent assay ; fermentation ; hepatotoxicity ; immunoaffinity chromatography ; nephrotoxicity ; peroxidase ; radishes ; rapid methods ; red yeast rice ; rice
    Language English
    Dates of publication 2022-0708
    Size p. 19981-19989.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/d2ra02823a
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Genistein protects against ultraviolet B–induced wrinkling and photoinflammation in in vitro and in vivo models

    Tang, Sheau-Chung / Hsiao, Yu-Ping / Ko, Jiunn-Liang

    Genes Nutr. 2022 Dec., v. 17, no. 1 p.4-4

    2022  

    Abstract: BACKGROUND: Chronic exposure to ultraviolet (UV) rays causes severe skin damage by inducing oxidative stress and inflammation. Identifying a safe and natural substance for skin protection is a crucial research goal. OBJECTIVE: The aim of this study was ... ...

    Abstract BACKGROUND: Chronic exposure to ultraviolet (UV) rays causes severe skin damage by inducing oxidative stress and inflammation. Identifying a safe and natural substance for skin protection is a crucial research goal. OBJECTIVE: The aim of this study was to clarify the effects of genistein on skin inflammation and photoaging by using 3 models (humans: skin parameters; animals: wrinkle formation; and cells: anti-inflammatory effects). METHODS: Food frequency questionnaire data and serum and skin parameter data from 120 volunteers (a group with a genistein-rich diet [RG group] and a control group). Human keratinocytes were pretreated with genistein before ultraviolet B (UVB) irradiation. Genistein was topically applied to the dorsal skin of rats. RESULTS: The blood samples of the RG group had lower serum uric acid levels and blood urea nitrogen levels. The dynamic elasticity level in the RG group was higher than that in the controls. Genistein pretreatment suppressed the expression of proinflammatory cytokines (CXCL1, IL-1, MIF, and PLANH1) and the proteins released by UVB-treated keratinocytes. Topical application of genistein to the dorsal skin of rats reduced the severity of UVB-induced wrinkling. Both intake and topical application of genistein combated UVB-induced inflammation and aging. CONCLUSIONS: Genistein could be used as a safe and natural compound for use in novel anti-inflammatory agents for topical application. The experimental design procedure, including the skin parameter and blood serum measurements of 137 participants. Genistein-rich compounds provide protection against UVB-induced inflammation, as determined using in vitro and in vivo animal model experiments.
    Keywords animal models ; blood serum ; chronic exposure ; experimental design ; food frequency questionnaires ; genistein ; humans ; inflammation ; interleukin-1 ; irradiation ; keratinocytes ; oxidative stress ; photoaging ; topical application ; ultraviolet radiation ; urea nitrogen ; uric acid
    Language English
    Dates of publication 2022-12
    Size p. 4.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2416599-2
    ISSN 1865-3499 ; 1555-8932
    ISSN (online) 1865-3499
    ISSN 1555-8932
    DOI 10.1186/s12263-022-00706-x
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Effect of combination treatment with Lactobacillus rhamnosus and corticosteroid in reducing airway inflammation in a mouse asthma model.

    Voo, Pit-Yee / Wu, Chia-Ta / Sun, Hai-Lun / Ko, Jiunn-Liang / Lue, Ko-Haung

    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

    2022  Volume 55, Issue 4, Page(s) 766–776

    Abstract: Background: Asthma is a complex multifactorial chronic airway inflammatory disease with diverse phenotypes and levels of severity and is associated with significant health and economic burden. In a certain population of asthma patients, the symptoms ... ...

    Abstract Background: Asthma is a complex multifactorial chronic airway inflammatory disease with diverse phenotypes and levels of severity and is associated with significant health and economic burden. In a certain population of asthma patients, the symptoms cannot be well controlled with steroid. There has been long standing interest in the use of probiotics for treating allergic diseases. The purpose of this study is to investigate whether the combination of Lactobacillus rhamnosus GG (LGG) with prednisolone could reduce the dosage of glucocorticoid in controlling airway inflammation in a murine model for allergic asthma.
    Material and methods: We used Der p 2-sensitized asthma model in female BALB/c mice. The animals were treated with 75 μl or 50 μl oral prednisolone or combination treatment of these two doses of oral prednisolone with LGG. Airway hyperresponsiveness, serum specific IgE/IgG1/IgG2a, infiltrating inflammatory cells in lung and cytokines were assessed.
    Results: Compared to 75 μl prednisolone, a lower dose of prednisolone with 50 μl was less satisfactory in suppressing airway hyperresponsives, serum IgE and IgG1, Th2 cytokines and inflammatory cytokines such as IL-6, IL-8 and IL-17 as well as infiltrating inflammatory cells. However, combination of 50 μl prednisolone and LGG decreased airway resistance and serum IgE and IgG1, inhibited the production of IL-4, IL-5, IL-6, IL-8, IL-13 and IL-17, upregulated serum IgG2a and enhanced Th1 immune response.
    Conclusions: LGG may reduce the dosage of prednisolone and thus may be beneficial in the treatment of asthma.
    MeSH term(s) Adrenal Cortex Hormones ; Animals ; Asthma ; Cytokines ; Disease Models, Animal ; Female ; Humans ; Immunoglobulin E ; Immunoglobulin G ; Inflammation ; Interleukin-17 ; Interleukin-6 ; Interleukin-8 ; Lactobacillus rhamnosus ; Mice ; Mice, Inbred BALB C ; Ovalbumin ; Prednisolone
    Chemical Substances Adrenal Cortex Hormones ; Cytokines ; Immunoglobulin G ; Interleukin-17 ; Interleukin-6 ; Interleukin-8 ; Immunoglobulin E (37341-29-0) ; Ovalbumin (9006-59-1) ; Prednisolone (9PHQ9Y1OLM)
    Language English
    Publishing date 2022-04-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1497590-7
    ISSN 1995-9133 ; 1684-1182 ; 0253-2662
    ISSN (online) 1995-9133
    ISSN 1684-1182 ; 0253-2662
    DOI 10.1016/j.jmii.2022.03.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Hydroxychloroquine repairs burn damage through the Wnt/β-catenin pathway.

    Tang, Sheau-Chung / Lu, Chun-Te / Ko, Jiunn-Liang / Lin, Cheng-Hui / Hsiao, Yu-Ping

    Chemico-biological interactions

    2022  Volume 370, Page(s) 110309

    MeSH term(s) Humans ; Hydroxychloroquine ; beta Catenin/metabolism ; Wnt Signaling Pathway ; Wnt Proteins/metabolism ; Burns
    Chemical Substances Hydroxychloroquine (4QWG6N8QKH) ; beta Catenin ; Wnt Proteins
    Language English
    Publishing date 2022-12-17
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 218799-1
    ISSN 1872-7786 ; 0009-2797
    ISSN (online) 1872-7786
    ISSN 0009-2797
    DOI 10.1016/j.cbi.2022.110309
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: β-catenin inhibitor ICG-001 suppress cell cycle progression and induce autophagy in endometrial cancer cells.

    Hsin, I-Lun / Wu, Pei-Ju / Tang, Sheau-Chung / Ou, Chu-Chyn / Chang, Hui-Yi / Shen, Huang-Pin / Ko, Jiunn-Liang / Wang, Po-Hui

    Journal of cellular physiology

    2023  Volume 238, Issue 10, Page(s) 2440–2450

    Abstract: The incidence of endometrial cancer has been rising in recent years. Gene mutation and high protein expression of β-catenin are commonly detected in endometrioid endometrial cancer. ICG-001 is a β-catenin inhibitor via blocking the complex formation of β- ...

    Abstract The incidence of endometrial cancer has been rising in recent years. Gene mutation and high protein expression of β-catenin are commonly detected in endometrioid endometrial cancer. ICG-001 is a β-catenin inhibitor via blocking the complex formation of β-catenin and cAMP response element-binding protein (CREB)-binding protein (CBP). This study aims to investigate the effect of ICG-001 on endometrial cancer inhibition. First, endometrial carcinoma patient-derived xenograft (PDX)-derived organoids and primary cells were used to verify the inhibiting ability of ICG-001 on endometrial cancer. Furthermore, endometrial cancer cell lines were used to investigate the anticancer mechanism of ICG-001. Using MTT assay and tumor spheroid formation assay, ICG-001 significantly reduced the cell viability of HEC-59 and HEC-1A cells. ICG-001 enhanced cisplatin-mediated cytotoxicity. ICG-001 decreased cancer stem cell sphere formation. ICG-001 decreased the protein expressions of CD44, hexokinase 2 (HK2), and cyclin A. ICG-001 lowered the cell cycle progression by flow cytometer analysis. Autophagy, but no apoptosis, was activated by ICG-001 in endometrial cancer cells. Autophagy inhibition by ATG5 silencing enhanced ICG-001-mediated suppression of cell viability, tumor spheroid formation, and protein expression of cyclin A and CD44. This study clarified the mechanism and revealed the clinical potential of ICG-001 against endometrial cancer.
    Language English
    Publishing date 2023-09-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3116-1
    ISSN 1097-4652 ; 0021-9541
    ISSN (online) 1097-4652
    ISSN 0021-9541
    DOI 10.1002/jcp.31103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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