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  1. AU="Kohsar, Matin"
  2. AU="Ella Brandt"
  3. AU="Jiwei Wang"
  4. AU="Croda, Júlio Henrique Rosa"
  5. AU="Arkun, Ani"
  6. AU="Fan-tao KONG"
  7. AU="Field, R C"
  8. AU="Coombes, Brandon J"
  9. AU="Garay, Raúl O."
  10. AU="Jingfeng Xue"
  11. AU="Kelliher, Christina M"
  12. AU="Busby, Marjorie G"

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  1. Artikel: Alternative Übertragungswege von Arboviren

    Kohsar, Matin / Kreuels, Benno

    Flugmedizin · Tropenmedizin · Reisemedizin - FTR

    2021  Band 28, Heft 01, Seite(n) 8–8

    Abstract: Das Denguevirus (DENV) ist ein humanpathogenes Virus aus der Familie der Flaviviren, welches schwere Verlaufsformen verursachen kann. Eine kausale Therapie existiert nicht, seit 2015 ist ein Impfstoff verfügbar, der allerdings ausschließlich für die ... ...

    Abstract Das Denguevirus (DENV) ist ein humanpathogenes Virus aus der Familie der Flaviviren, welches schwere Verlaufsformen verursachen kann. Eine kausale Therapie existiert nicht, seit 2015 ist ein Impfstoff verfügbar, der allerdings ausschließlich für die Impfung der Bevölkerung von hochendemischen Gebieten empfohlen wird 1. Die Übertragung erfolgt, wie bei dem verwandten Zikavirus (ZIKV), primär über Stiche von infizierten Aedes-Mücken. Nachdem bei ZIKV die sexuelle Transmission als relevante Übertragungsroute nachgewiesen wurde, stellt sich die Frage, ob auch DENV sexuell übertragen wird.:
    Sprache Deutsch
    Erscheinungsdatum 2021-02-01
    Verlag Georg Thieme Verlag KG
    Erscheinungsort Stuttgart ; New York
    Dokumenttyp Artikel
    ZDB-ID 2277004-5
    ISSN 1864-175X ; 1864-4538
    ISSN (online) 1864-175X
    ISSN 1864-4538
    DOI 10.1055/a-1253-9975
    Datenquelle Thieme Verlag

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  2. Artikel ; Online: Human hepatitis D virus-specific T cell epitopes.

    Kohsar, Matin / Landahl, Johanna / Neumann-Haefelin, Christoph / Schulze Zur Wiesch, Julian

    JHEP reports : innovation in hepatology

    2021  Band 3, Heft 4, Seite(n) 100294

    Abstract: HDV is a small, defective RNA virus that requires the HBsAg of HBV for its assembly, release, and transmission. Chronic HBV/HDV infection often has a severe clinical outcome and is difficult to treat. The important role of a robust virus-specific T cell ... ...

    Abstract HDV is a small, defective RNA virus that requires the HBsAg of HBV for its assembly, release, and transmission. Chronic HBV/HDV infection often has a severe clinical outcome and is difficult to treat. The important role of a robust virus-specific T cell response for natural viral control has been established for many other chronic viral infections, but the exact role of the T cell response in the control and progression of chronic HDV infection is far less clear. Several recent studies have characterised HDV-specific CD4+ and CD8+ T cell responses on a peptide level. This review comprehensively summarises all HDV-specific T cell epitopes described to date and describes our current knowledge of the role of T cells in HDV infection. While we now have better tools to study the adaptive anti-HDV-specific T cell response, further efforts are needed to define the HLA restriction of additional HDV-specific T cell epitopes, establish additional HDV-specific MHC tetramers, understand the degree of cross HDV genotype reactivity of individual epitopes and understand the correlation of the HBV- and HDV-specific T cell response, as well as the breadth and specificity of the intrahepatic HDV-specific T cell response.
    Sprache Englisch
    Erscheinungsdatum 2021-04-23
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Review
    ISSN 2589-5559
    ISSN (online) 2589-5559
    DOI 10.1016/j.jhepr.2021.100294
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Pathogens associated with hospitalization due to acute lower respiratory tract infections in children in rural Ghana: a case-control study.

    Krumkamp, Ralf / Kohsar, Matin / Nolte, Kolja / Hogan, Benedikt / Eibach, Daniel / Jaeger, Anna / Akenten, Charity Wiafe / Drosten, Christian / Boahen, Kennedy Gyau / Sarpong, Nimako / Eckerle, Isabella / Binger, Tabea / Owusu-Dabo, Ellis / May, Jürgen / Kreuels, Benno

    Scientific reports

    2023  Band 13, Heft 1, Seite(n) 2443

    Abstract: Respiratory infections are one of the most common causes of death among children under the age of five years. Data on prevalence and relevance of specific organisms in African children are still lacking. This case-control-study investigated prevalence ... ...

    Abstract Respiratory infections are one of the most common causes of death among children under the age of five years. Data on prevalence and relevance of specific organisms in African children are still lacking. This case-control-study investigated prevalence and relevance of specific organisms in Ghanaian children admitted to hospital with symptoms of lower respiratory tract infection (LRTI). Pharyngeal swabs were taken and tested by PCR for 19 respiratory isolates. Adjusted odds ratios (aORs) were calculated to estimate associations between isolates and admission with LRTI. Population attributable fractions (PAFs) were calculated to assess the proportion of LRTI cases due to a particular pathogen. The study included 327 cases and 562 controls. We found associations between detection and admission for LRTI for influenza (aOR 98.6; 95% confidence interval (CI) 20.0-1789.6), respiratory syncytial virus (aOR 40.2; 95% CI 7.2-758.6), H. influenzae (aOR 4.1; 95% CI 2.2-7.9) and S. pneumoniae (aOR 2.4; 95% CI 1.7-3.4). PAFs ≥ 10% were observed for S. pneumoniae (30%; 95% CI 26-42), H. influenzae (10%; 95% CI 2-19) and influenza (10%; 95% CI 2-18). This study highlights the need for heightened surveillance and development of effective vaccines for respiratory pathogens other than SARS-CoV-2 in the future.
    Mesh-Begriff(e) Humans ; Child ; Infant ; Child, Preschool ; Ghana/epidemiology ; Influenza, Human/epidemiology ; Case-Control Studies ; COVID-19 ; SARS-CoV-2 ; Respiratory Tract Infections/epidemiology ; Respiratory Syncytial Virus, Human ; Streptococcus pneumoniae ; Haemophilus influenzae ; Hospitalization ; Respiratory Syncytial Virus Infections/epidemiology
    Sprache Englisch
    Erscheinungsdatum 2023-02-10
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-29410-5
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: High Rates of Liver Cirrhosis and Hepatocellular Carcinoma in Chronic Hepatitis B Patients with Metabolic and Cardiovascular Comorbidities.

    Bockmann, Jan-Hendrik / Kohsar, Matin / Murray, John M / Hamed, Vanessa / Dandri, Maura / Lüth, Stefan / Lohse, Ansgar W / Schulze-Zur-Wiesch, Julian

    Microorganisms

    2021  Band 9, Heft 5

    Abstract: Background: The prevalence of metabolic and cardiovascular diseases is rising worldwide. However, little is known about the impact of such disorders on hepatic disease progression in chronic hepatitis B (CHB) during the era of potent nucleo(s)tide ... ...

    Abstract Background: The prevalence of metabolic and cardiovascular diseases is rising worldwide. However, little is known about the impact of such disorders on hepatic disease progression in chronic hepatitis B (CHB) during the era of potent nucleo(s)tide analogues (NAs).
    Methods: We retrospectively analyzed a single-center cohort of 602 CHB patients, comparing the frequency of liver cirrhosis at baseline and incidences of liver-related events during follow-up (hepatocellular carcinoma, liver transplantation and liver-related death) between CHB patients with a history of diabetes, obesity, hypertension or coronary heart disease (CHD).
    Results: Rates of cirrhosis at baseline and liver-related events during follow-up (median follow-up time: 2.51 years; NA-treated: 37%) were substantially higher in CHB patients with diabetes (11/23; 3/23), obesity (6/13; 2/13), CHD (7/11; 2/11) or hypertension (15/43; 4/43) compared to CHB patients without the indicated comorbidities (26/509; 6/509). Multivariate analysis identified diabetes as the most significant predictor for cirrhosis (
    Conclusion: The combination of metabolic diseases and CHB is associated with substantially increased rates of liver cirrhosis and secondary liver-related events compared to CHB alone, indicating that hepatitis B patients with metabolic comorbidities warrant particular attention in disease surveillance and evaluation of treatment indication.
    Sprache Englisch
    Erscheinungsdatum 2021-04-30
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9050968
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: High Rates of Liver Cirrhosis and Hepatocellular Carcinoma in Chronic Hepatitis B Patients with Metabolic and Cardiovascular Comorbidities

    Bockmann, Jan-Hendrik / Kohsar, Matin / Murray, John M / Hamed, Vanessa / Dandri, Maura / Lüth, Stefan / Lohse, Ansgar W / Schulze-zur-Wiesch, Julian

    Microorganisms. 2021 Apr. 30, v. 9, no. 5

    2021  

    Abstract: Background: The prevalence of metabolic and cardiovascular diseases is rising worldwide. However, little is known about the impact of such disorders on hepatic disease progression in chronic hepatitis B (CHB) during the era of potent nucleo(s)tide ... ...

    Abstract Background: The prevalence of metabolic and cardiovascular diseases is rising worldwide. However, little is known about the impact of such disorders on hepatic disease progression in chronic hepatitis B (CHB) during the era of potent nucleo(s)tide analogues (NAs). Methods: We retrospectively analyzed a single-center cohort of 602 CHB patients, comparing the frequency of liver cirrhosis at baseline and incidences of liver-related events during follow-up (hepatocellular carcinoma, liver transplantation and liver-related death) between CHB patients with a history of diabetes, obesity, hypertension or coronary heart disease (CHD). Results: Rates of cirrhosis at baseline and liver-related events during follow-up (median follow-up time: 2.51 years; NA-treated: 37%) were substantially higher in CHB patients with diabetes (11/23; 3/23), obesity (6/13; 2/13), CHD (7/11; 2/11) or hypertension (15/43; 4/43) compared to CHB patients without the indicated comorbidities (26/509; 6/509). Multivariate analysis identified diabetes as the most significant predictor for cirrhosis (p = 0.0105), while comorbidities did not correlate with liver-related events in pre-existing cirrhosis. Conclusion: The combination of metabolic diseases and CHB is associated with substantially increased rates of liver cirrhosis and secondary liver-related events compared to CHB alone, indicating that hepatitis B patients with metabolic comorbidities warrant particular attention in disease surveillance and evaluation of treatment indication.
    Schlagwörter chronic hepatitis B ; coronary disease ; death ; diabetes ; disease progression ; disease surveillance ; hepatoma ; hypertension ; liver cirrhosis ; liver transplant ; multivariate analysis ; obesity
    Sprache Englisch
    Erscheinungsverlauf 2021-0430
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9050968
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel ; Online: Correction: Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19.

    Heide, Janna / Schulte, Sophia / Kohsar, Matin / Brehm, Thomas Theo / Herrmann, Marissa / Karsten, Hendrik / Marget, Matthias / Peine, Sven / Johansson, Alexandra M / Sette, Alessandro / Lütgehetmann, Marc / Kwok, William W / Sidney, John / Schulze Zur Wiesch, Julian

    PLoS pathogens

    2022  Band 18, Heft 1, Seite(n) e1010220

    Abstract: This corrects the article DOI: 10.1371/journal.ppat.1009842.]. ...

    Abstract [This corrects the article DOI: 10.1371/journal.ppat.1009842.].
    Sprache Englisch
    Erscheinungsdatum 2022-01-05
    Erscheinungsland United States
    Dokumenttyp Published Erratum
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1010220
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Tecovirimat therapy for severe monkeypox infection: Longitudinal assessment of viral titers and clinical response pattern-A first case-series experience.

    Hermanussen, Lennart / Grewe, Ilka / Tang, Hui Ting / Nörz, Dominik / Bal, Lukas C / Pfefferle, Susanne / Unger, Stefan / Hoffmann, Christian / Berzow, Dirk / Kohsar, Matin / Aepfelbacher, Martin / Lohse, Ansgar W / Addo, Marylyn M / Lütgehetmann, Marc / Schulze Zur Wiesch, Julian / Schmiedel, Stefan

    Journal of medical virology

    2022  Band 95, Heft 1, Seite(n) e28181

    Mesh-Begriff(e) Humans ; Mpox (monkeypox)/diagnosis ; Mpox (monkeypox)/drug therapy
    Sprache Englisch
    Erscheinungsdatum 2022-10-20
    Erscheinungsland United States
    Dokumenttyp Letter
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.28181
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

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  8. Artikel ; Online: Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19.

    Heide, Janna / Schulte, Sophia / Kohsar, Matin / Brehm, Thomas Theo / Herrmann, Marissa / Karsten, Hendrik / Marget, Matthias / Peine, Sven / Johansson, Alexandra M / Sette, Alessandro / Lütgehetmann, Marc / Kwok, William W / Sidney, John / Schulze Zur Wiesch, Julian

    PLoS pathogens

    2021  Band 17, Heft 9, Seite(n) e1009842

    Abstract: The aim of this study was to define the breadth and specificity of dominant SARS-CoV-2-specific T cell epitopes using a comprehensive set of 135 overlapping 15-mer peptides covering the SARS-CoV-2 envelope (E), membrane (M) and nucleoprotein (N) in a ... ...

    Abstract The aim of this study was to define the breadth and specificity of dominant SARS-CoV-2-specific T cell epitopes using a comprehensive set of 135 overlapping 15-mer peptides covering the SARS-CoV-2 envelope (E), membrane (M) and nucleoprotein (N) in a cohort of 34 individuals with acute (n = 10) and resolved (n = 24) COVID-19. Following short-term virus-specific in vitro cultivation, the single peptide-specific CD4+ T cell response of each patient was screened using enzyme linked immuno spot assay (ELISpot) and confirmed by single-peptide intracellular cytokine staining (ICS) for interferon-γ (IFN-γ) production. 97% (n = 33) of patients elicited one or more N, M or E-specific CD4+ T cell responses and each patient targeted on average 21.7 (range 0-79) peptide specificities. Overall, we identified 10 N, M or E-specific peptides that showed a response frequency of more than 36% and five of them showed high binding affinity to multiple HLA class II binders in subsequent in vitro HLA binding assays. Three peptides elicited CD4+ T cell responses in more than 55% of all patients, namely Mem_P30 (aa146-160), Mem_P36 (aa176-190), both located within the M protein, and Ncl_P18 (aa86-100) located within the N protein. These peptides were further defined in terms of length and HLA restriction. Based on this epitope and restriction data we developed a novel DRB*11 tetramer (Mem_aa145-164) and examined the ex vivo phenotype of SARS-CoV-2-specific CD4+ T cells in one patient. This detailed characterization of single T cell peptide responses demonstrates that SARS-CoV-2 infection universally primes a broad T cell response directed against multiple specificities located within the N, M and E structural protein.
    Mesh-Begriff(e) Acute Disease ; Adult ; Aged ; CD4-Positive T-Lymphocytes/immunology ; COVID-19/immunology ; Cohort Studies ; Coronavirus Envelope Proteins/immunology ; Coronavirus Nucleocapsid Proteins/immunology ; Enzyme-Linked Immunospot Assay ; Epitopes, T-Lymphocyte/immunology ; Female ; Humans ; Male ; Middle Aged ; Phosphoproteins/immunology ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus/immunology ; Survivors ; T-Cell Antigen Receptor Specificity ; Viral Matrix Proteins/immunology
    Chemische Substanzen Coronavirus Envelope Proteins ; Coronavirus Nucleocapsid Proteins ; Epitopes, T-Lymphocyte ; Phosphoproteins ; Spike Glycoprotein, Coronavirus ; Viral Matrix Proteins ; envelope protein, SARS-CoV-2 ; membrane protein, SARS-CoV-2 ; nucleocapsid phosphoprotein, SARS-CoV-2 ; spike protein, SARS-CoV-2
    Sprache Englisch
    Erscheinungsdatum 2021-09-16
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1009842
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Defining the CD39/CD73 Axis in SARS-CoV-2 Infection: The CD73

    Ahmadi, Parimah / Hartjen, Philip / Kohsar, Matin / Kummer, Silke / Schmiedel, Stefan / Bockmann, Jan-Hendrik / Fathi, Anahita / Huber, Samuel / Haag, Friedrich / Schulze Zur Wiesch, Julian

    Cells

    2020  Band 9, Heft 8

    Abstract: The ectonucleotidases CD39 and CD73 regulate immune responses by balancing extracellular ATP and adenosine in inflammation and are likely to be involved in the pathophysiology of COVID-19. Here, we analyzed CD39 and CD73 on different lymphocyte ... ...

    Abstract The ectonucleotidases CD39 and CD73 regulate immune responses by balancing extracellular ATP and adenosine in inflammation and are likely to be involved in the pathophysiology of COVID-19. Here, we analyzed CD39 and CD73 on different lymphocyte populations in a small cohort of COVID-19 patients and in healthy individuals. We describe a significantly lower level of expression of CD73 on cytotoxic lymphocyte populations, including CD8
    Mesh-Begriff(e) 5'-Nucleotidase/metabolism ; Adenosine/metabolism ; Adult ; Aged ; Apyrase/metabolism ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/enzymology ; Coronavirus Infections/immunology ; Female ; GPI-Linked Proteins/metabolism ; Granzymes/metabolism ; Humans ; Inflammation/enzymology ; Inflammation/immunology ; Interferon-gamma/metabolism ; Killer Cells, Natural/immunology ; Male ; Middle Aged ; Natural Killer T-Cells/immunology ; Pandemics ; Perforin/metabolism ; Pneumonia, Viral/enzymology ; Pneumonia, Viral/immunology ; SARS-CoV-2 ; Signal Transduction/immunology ; T-Lymphocytes, Cytotoxic/immunology ; T-Lymphocytes, Cytotoxic/metabolism ; Tumor Necrosis Factor-alpha/metabolism
    Chemische Substanzen GPI-Linked Proteins ; Tumor Necrosis Factor-alpha ; Perforin (126465-35-8) ; Interferon-gamma (82115-62-6) ; 5'-Nucleotidase (EC 3.1.3.5) ; NT5E protein, human (EC 3.1.3.5) ; Granzymes (EC 3.4.21.-) ; Apyrase (EC 3.6.1.5) ; ENTPD1 protein, human (EC 3.6.1.5) ; Adenosine (K72T3FS567)
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-07-22
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells9081750
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Control of SARS-CoV-2 infection in rituximab-treated neuroimmunological patients.

    Woo, Marcel S / Steins, David / Häußler, Vivien / Kohsar, Matin / Haag, Friedrich / Elias-Hamp, Birte / Heesen, Christoph / Lütgehetmann, Marc / Schulze Zur Wiesch, Julian / Friese, Manuel A

    Journal of neurology

    2020  Band 268, Heft 1, Seite(n) 5–7

    Mesh-Begriff(e) Adult ; Aged ; Antibodies, Viral/blood ; B-Lymphocytes ; COVID-19/blood ; COVID-19/complications ; COVID-19/therapy ; Female ; Humans ; Immunologic Factors/therapeutic use ; Multiple Sclerosis, Relapsing-Remitting/drug therapy ; Neuromyelitis Optica/drug therapy ; Rituximab/therapeutic use
    Chemische Substanzen Antibodies, Viral ; Immunologic Factors ; Rituximab (4F4X42SYQ6)
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-07-11
    Erscheinungsland Germany
    Dokumenttyp Case Reports ; Letter
    ZDB-ID 187050-6
    ISSN 1432-1459 ; 0340-5354 ; 0012-1037 ; 0939-1517 ; 1619-800X
    ISSN (online) 1432-1459
    ISSN 0340-5354 ; 0012-1037 ; 0939-1517 ; 1619-800X
    DOI 10.1007/s00415-020-10046-8
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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