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  1. AU="Koji Ueda"
  2. AU="Pillas, Diana J"
  3. AU="Thomson, Jason J"
  4. AU="Mitra, Kalyan"
  5. AU="Sanjay Desai"
  6. AU=Cox David J AU=Cox David J
  7. AU="Grebenok, Robert J."
  8. AU="Blackburne, Brittney"
  9. AU="Bortoleti, Bruna Taciane da Silva"
  10. AU="Ehrbar, Martin"
  11. AU="Lepre, Davide"
  12. AU="Olszewska, Zuzanna"
  13. AU="Vojta, Leslie"
  14. AU=Wickstrom Eric AU=Wickstrom Eric
  15. AU="Gangavarapu, Sridevi"
  16. AU="Hussein, Hazem Abdelwaheb"
  17. AU=Cai Yixin AU=Cai Yixin
  18. AU="Hüls, Anke"
  19. AU="Poondru, Srinivasu"
  20. AU="Coca, Daniel"
  21. AU="Lebeau, Paul"
  22. AU="Dehghani, Sedigheh"
  23. AU="Ishibashi, Kenji"
  24. AU="Xu, Yanhua"
  25. AU="Matera, Katarzyna"
  26. AU="Ait-Ouarab, Slimane"
  27. AU="Nicola, Coppede"
  28. AU="Dewitt, John M"
  29. AU="Sorin M. Dudea"
  30. AU="Tanusha D. Ramdin"
  31. AU="Hao, Zehui"
  32. AU="Chauhan, Aman"

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  1. Artikel ; Online: Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers

    Toshifumi Hara / Yuuki Tominaga / Koji Ueda / Keichiro Mihara / Kazuyoshi Yanagihara / Yoshifumi Takei

    Biochemistry and Biophysics Reports, Vol 30, Iss , Pp 101255- (2022)

    2022  

    Abstract: Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can ... ...

    Abstract Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can lead to various diseases including cancers, little is known about the relationship between methyltransferase and cancers. Here we aimed to understand the role of methyltransferase in cancer metastasis. We found that elevated methyltransferase-like 9 (METTL9) is closely associated with the acquisition of metastatic activity in human scirrhous gastric cancers. The stable knockdown of METTL9 via an shRNA vector technique in our original metastatic cells from scirrhous gastric cancer patients significantly inhibited migration and invasion. In metastatic cells, METTL9 protein is predominantly localized in mitochondria, and the METTL9 knockdown significantly reduced mitochondrial Complex I activity. METTL9 can be a candidate of molecular targets to inhibit peritoneal dissemination of scirrhous gastric cancers. This report is the first to describe the relationship between METTL9 and cancer metastasis.
    Schlagwörter Scirrhous gastric cancer ; Peritoneal dissemination ; Methyltransferase-like (METTL) gene ; Short hairpin RNA ; Molecular targeting therapy against cancer metastasis ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Thema/Rubrik (Code) 612
    Sprache Englisch
    Erscheinungsdatum 2022-07-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Specimen-specific drift of densities defines distinct subclasses of extracellular vesicles from human whole saliva.

    Satoshi Yamamoto / Kohji Okamura / Risa Fujii / Takamasa Kawano / Koji Ueda / Yasutomo Yajima / Kiyotaka Shiba

    PLoS ONE, Vol 16, Iss 4, p e

    2021  Band 0249526

    Abstract: Extracellular vesicles (EVs) in body fluids constitute heterogenous populations, which mirror their diverse parental cells as well as distinct EV-generation pathways. Various methodologies have been proposed to differentiate EVs in order to deepen the ... ...

    Abstract Extracellular vesicles (EVs) in body fluids constitute heterogenous populations, which mirror their diverse parental cells as well as distinct EV-generation pathways. Various methodologies have been proposed to differentiate EVs in order to deepen the current understanding of EV biology. Equilibrium density-gradient centrifugation has often been used to separate EVs based on their buoyant densities; however, the standard conditions used for the method do not necessarily allow all EVs to move to their equilibrium density positions, which complicates the categorization of EVs. Here, by prolonging ultracentrifugation time to 96 h and fractionating EVs both by floating up or spinning down directions, we allowed 111 EV-associated protein markers from the whole saliva of three healthy volunteers to attain equilibrium. Interestingly, the determined buoyant densities of the markers drifted in a specimen-specific manner, and drift patterns differentiated EVs into at least two subclasses. One class carried classical exosomal markers, such as CD63 and CD81, and the other was characterized by the molecules involved in membrane remodeling or vesicle trafficking. Distinct patterns of density drift may represent the differences in generation pathways of EVs.
    Schlagwörter Medicine ; R ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: The Patterning and Proportion of Charged Residues in the Arginine-Rich Mixed-Charge Domain Determine the Membrane-Less Organelle Targeted by the Protein

    Tamami Miyagi / Rio Yamazaki / Koji Ueda / Satoshi Narumi / Yuhei Hayamizu / Hiroshi Uji-i / Masahiko Kuroda / Kohsuke Kanekura

    International Journal of Molecular Sciences, Vol 23, Iss 14, p

    2022  Band 7658

    Abstract: Membrane-less organelles (MLOs) are formed by biomolecular liquid–liquid phase separation (LLPS). Proteins with charged low-complexity domains (LCDs) are prone to phase separation and localize to MLOs, but the mechanism underlying the distributions of ... ...

    Abstract Membrane-less organelles (MLOs) are formed by biomolecular liquid–liquid phase separation (LLPS). Proteins with charged low-complexity domains (LCDs) are prone to phase separation and localize to MLOs, but the mechanism underlying the distributions of such proteins to specific MLOs remains poorly understood. Recently, proteins with Arg-enriched mixed-charge domains (R-MCDs), primarily composed of R and Asp (D), were found to accumulate in nuclear speckles via LLPS. However, the process by which R-MCDs selectively incorporate into nuclear speckles is unknown. Here, we demonstrate that the patterning of charged amino acids and net charge determines the targeting of specific MLOs, including nuclear speckles and the nucleolus, by proteins. The redistribution of R and D residues from an alternately sequenced pattern to uneven blocky sequences caused a shift in R-MCD distribution from nuclear speckles to the nucleolus. In addition, the incorporation of basic residues in the R-MCDs promoted their localization to the MLOs and their apparent accumulation in the nucleolus. The R-MCD peptide with alternating amino acids did not undergo LLPS, whereas the blocky R-MCD peptide underwent LLPS with affinity to RNA, acidic poly-Glu, and the acidic nucleolar protein nucleophosmin, suggesting that the clustering of R residues helps avoid their neutralization by D residues and eventually induces R-MCD migration to the nucleolus. Therefore, the distribution of proteins to nuclear speckles requires the proximal positioning of D and R for the mutual neutralization of their charges.
    Schlagwörter liquid–liquid phase separation ; membrane-less organelle ; nuclear speckle ; nucleolus ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 612
    Sprache Englisch
    Erscheinungsdatum 2022-07-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges

    Tamami Miyagi / Koji Ueda / Masahiro Sugimoto / Takuya Yagi / Daisuke Ito / Rio Yamazaki / Satoshi Narumi / Yuhei Hayamizu / Hiroshi Uji-i / Masahiko Kuroda / Kohsuke Kanekura

    iScience, Vol 26, Iss 6, Pp 106957- (2023)

    2023  

    Abstract: Summary: Arginine-rich dipeptide repeat proteins (R-DPRs), poly(PR) and poly(GR), translated from the hexanucleotide repeat expansion in the amyotrophic lateral sclerosis (ALS)-causative C9ORF72 gene, contribute significantly to pathogenesis of ALS. ... ...

    Abstract Summary: Arginine-rich dipeptide repeat proteins (R-DPRs), poly(PR) and poly(GR), translated from the hexanucleotide repeat expansion in the amyotrophic lateral sclerosis (ALS)-causative C9ORF72 gene, contribute significantly to pathogenesis of ALS. Although both R-DPRs share many similarities, there are critical differences in their subcellular localization, phase separation, and toxicity mechanisms. We analyzed localization, protein-protein interactions, and phase separation of R-DPR variants and found that sufficient segregation of arginine charges is necessary for nucleolar distribution. Proline not only efficiently separated the charges, but also allowed for weak, but highly multivalent binding. In contrast, because of its high flexibility, glycine cannot fully separate the charges, and poly(GR) behaves similarly to the contiguous arginines, being trapped in the cytoplasm. We conclude that the amino acid that spaces the arginine charges determines the strength and multivalency of the binding, leading to differences in localization and toxicity mechanisms.
    Schlagwörter Molecular biology ; Neuroscience ; Science ; Q
    Thema/Rubrik (Code) 571
    Sprache Englisch
    Erscheinungsdatum 2023-06-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
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  5. Artikel ; Online: Identification of Novel Senescent Markers in Small Extracellular Vesicles

    Tomoka Misawa / Kazuhiro Hitomi / Kenichi Miyata / Yoko Tanaka / Risa Fujii / Masatomo Chiba / Tze Mun Loo / Aki Hanyu / Hiroko Kawasaki / Hisaya Kato / Yoshiro Maezawa / Koutaro Yokote / Asako J. Nakamura / Koji Ueda / Nobuo Yaegashi / Akiko Takahashi

    International Journal of Molecular Sciences, Vol 24, Iss 2421, p

    2023  Band 2421

    Abstract: Senescent cells exhibit several typical features, including the senescence-associated secretory phenotype (SASP), promoting the secretion of various inflammatory proteins and small extracellular vesicles (EVs). SASP factors cause chronic inflammation, ... ...

    Abstract Senescent cells exhibit several typical features, including the senescence-associated secretory phenotype (SASP), promoting the secretion of various inflammatory proteins and small extracellular vesicles (EVs). SASP factors cause chronic inflammation, leading to age-related diseases. Recently, therapeutic strategies targeting senescent cells, known as senolytics, have gained attention; however, noninvasive methods to detect senescent cells in living organisms have not been established. Therefore, the goal of this study was to identify novel senescent markers using small EVs (sEVs). sEVs were isolated from young and senescent fibroblasts using three different methods, including size-exclusion chromatography, affinity column for phosphatidylserine, and immunoprecipitation using antibodies against tetraspanin proteins, followed by mass spectrometry. Principal component analysis revealed that the protein composition of sEVs released from senescent cells was significantly different from that of young cells. Importantly, we identified ATP6V0D1 and RTN4 as novel markers that are frequently upregulated in sEVs from senescent and progeria cells derived from patients with Werner syndrome. Furthermore, these two proteins were significantly enriched in sEVs from the serum of aged mice. This study supports the potential use of senescent markers from sEVs to detect the presence of senescent cells in vivo.
    Schlagwörter biomarker ; cellular senescence ; EV proteomics ; SASP ; Werner syndrome ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2023-01-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Dosimetric comparison of five different radiotherapy treatment planning approaches for locally advanced non-small cell lung cancer with sequential plan changes.

    Saito, Masahide / Komiyama, Takafumi / Marino, Kan / Aoki, Shinichi / Akita, Tomoko / Matsuda, Masaki / Sano, Naoki / Suzuki, Hidekazu / Koji, Ueda / Nemoto, Hikaru / Onishi, Hiroshi

    Thoracic cancer

    2023  Band 14, Heft 35, Seite(n) 3445–3452

    Abstract: Background: The purpose of this study was to compare the dosimetric characteristics of five different treatment planning techniques for locally advanced non-small cell lung cancer (LA-NSCLC) with sequential plan changes.: Methods: A total of 13 stage ...

    Abstract Background: The purpose of this study was to compare the dosimetric characteristics of five different treatment planning techniques for locally advanced non-small cell lung cancer (LA-NSCLC) with sequential plan changes.
    Methods: A total of 13 stage III NSCLC patients were enrolled in this study. These patients had both computed tomography (CT) images for initial and boost treatment plans. The latter CT images were taken if tumor shrinkage was observed after 2 weeks of treatment. The prescription dose was 60 Gy/30 Fr (initial: 40 Gy/20 Fr, and boost: 20 Gy/10 Fr). Five techniques (forward-planed 3-dimensional conformal radiotherapy [F-3DCRT] on both CT images, inverse-planned 3DCRT [I-3DCRT] on both CT images, volumetric modulated arc therapy [VMAT] on both CT images, F-3DCRT on initial CT plus VMAT on boost CT [bVMAT], and hybrid of fixed intensity-modulated radiotherapy [IMRT] beams and VMAT beams on both CT images [hybrid]) were recalculated for all patients. The accumulated doses between initial and boost plans were compared among all treatment techniques.
    Results: The conformity indexes (CI) of the planning target volume (PTV) of the five planning techniques were 0.34 ± 0.10, 0.57 ± 0.10, 0.86 ± 0.08, 0.61 ± 0.12, and 0.83 ± 0.11 for F-3DCRT, I-3DCRT, VMAT, bVMAT, and hybrid, respectively. In the same manner, lung volumes receiving >20 Gy (V
    Conclusion: The IMRT/VMAT hybrid technique for LA-NSCLC patients improved target CI and reduced lung doses. Furthermore, if IMRT was not available initially, starting with 3DCRT might be beneficial as demonstrated in the bVMAT procedure of this study.
    Mesh-Begriff(e) Humans ; Carcinoma, Non-Small-Cell Lung/radiotherapy ; Carcinoma, Non-Small-Cell Lung/etiology ; Radiotherapy Planning, Computer-Assisted/methods ; Radiotherapy Dosage ; Lung Neoplasms/radiotherapy ; Lung Neoplasms/etiology ; Radiotherapy, Conformal/methods
    Sprache Englisch
    Erscheinungsdatum 2023-10-16
    Erscheinungsland Singapore
    Dokumenttyp Journal Article
    ZDB-ID 2625856-0
    ISSN 1759-7714 ; 1759-7706
    ISSN (online) 1759-7714
    ISSN 1759-7706
    DOI 10.1111/1759-7714.15137
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Development and Evaluation of a Robust Sandwich Immunoassay System Detecting Serum WFA-Reactive IgA1 for Diagnosis of IgA Nephropathy

    Yuta Uenoyama / Atsushi Matsuda / Kazune Ohashi / Koji Ueda / Misaki Yokoyama / Takuya Kyoutou / Kouji Kishi / Youichi Takahama / Masaaki Nagai / Takaaki Ohbayashi / Osamu Hotta / Hideki Matsuzaki

    International Journal of Molecular Sciences, Vol 23, Iss 5165, p

    2022  Band 5165

    Abstract: Aberrant glycosylation of IgA1 is involved in the development of IgA nephropathy (IgAN). There are many reports of IgAN markers focusing on the glycoform of IgA1. None have been clinically applied as a routine test. In this study, we established an ... ...

    Abstract Aberrant glycosylation of IgA1 is involved in the development of IgA nephropathy (IgAN). There are many reports of IgAN markers focusing on the glycoform of IgA1. None have been clinically applied as a routine test. In this study, we established an automated sandwich immunoassay system for detecting aberrant glycosylated IgA1, using Wisteria floribunda agglutinin (WFA) and anti-IgA1 monoclonal antibody. The diagnostic performance as an IgAN marker was evaluated. The usefulness of WFA for immunoassays was investigated by lectin microarray. A reliable standard for quantitative immunoassay measurements was designed by modifying a purified IgA1 substrate. A validation study using multiple serum specimens was performed using the established WFA-antibody sandwich automated immunoassay. Lectin microarray results showed that WFA specifically recognized N -glycans of agglutinated IgA1 in IgAN patients. The constructed IgA1 standard exhibited a wide dynamic range and high reactivity. In the validation study, serum WFA-reactive IgA1 (WFA+-IgA1) differed significantly between healthy control subjects and IgAN patients. The findings indicate that WFA is a suitable lectin that specifically targets abnormal agglutinated IgA1 in serum. We also describe an automated immunoassay system for detecting WFA+-IgA1, focusing on N-glycans.
    Schlagwörter IgA nephropathy ; immunocomplex ; glyco-diagnosis ; lectin ; lectin microarray ; automated immunoassay ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Sprache Englisch
    Erscheinungsdatum 2022-05-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Comprehensive Proteomic Profiling of Vitreous Humor in Ocular Sarcoidosis Compared with Other Vitreoretinal Diseases

    Hiroyuki Komatsu / Yoshihiko Usui / Kinya Tsubota / Risa Fujii / Takefumi Yamaguchi / Kazuichi Maruyama / Ryo Wakita / Masaki Asakage / Hiroyuki Shimizu / Naoyuki Yamakawa / Naoya Nezu / Koji Ueda / Hiroshi Goto

    Journal of Clinical Medicine, Vol 11, Iss 3606, p

    2022  Band 3606

    Abstract: Ocular sarcoidosis is an inflammatory disease that manifests as uveitis, and is often difficult to distinguish from other forms of uveitis based on nonspecific findings alone. Comprehensive proteomic analyses of vitreous humor using LC-MS/MS were ... ...

    Abstract Ocular sarcoidosis is an inflammatory disease that manifests as uveitis, and is often difficult to distinguish from other forms of uveitis based on nonspecific findings alone. Comprehensive proteomic analyses of vitreous humor using LC-MS/MS were performed in each patient with ocular sarcoidosis, vitreoretinal lymphoma (VRL), and controls with epiretinal membrane or macular hole. Differential expression proteins (DEPs) were identified by comparing with VRL and controls, and functional pathway analysis was performed. The candidate biomarker proteins for ocular sarcoidosis were validated using enzyme-linked immunosorbent assay. A total of 1590 proteins were identified in all samples. Of these, 290 and 174 DEPs were detected in vitreous of ocular sarcoidosis compared with controls and VRL, respectively. Enrichment pathway analysis revealed that pathways related to the immune system were most upregulated. Validation of two candidate biomarkers for ocular sarcoidosis, neutrophil gelatinase-associated lipocalin (NGAL) and junctional adhesion molecules B (JAMB), confirmed upregulated NGAL and JAMB protein expressions in ocular sarcoidosis compared to controls and VRL. The results of this study revealed that altered vitreous protein expression levels may discriminate ocular sarcoidosis from other uveitis diseases. Vitreous NGAL and JAMB are potential biomarkers and may serve as an auxiliary tool for the diagnosis of ocular sarcoidosis.
    Schlagwörter proteomics analysis ; ocular sarcoidosis ; uveitis ; vitreoretinal diseases ; vitreoretinal lymphoma ; Medicine ; R
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2022-06-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel ; Online: Polarity protein SCRIB interacts with SLC3A2 to regulate proliferation and tamoxifen resistance in ER+ breast cancer

    Yasuhiro Saito / Shiori Matsuda / Naomi Ohnishi / Keiko Endo / Sanae Ashitani / Maki Ohishi / Ayano Ueno / Masaru Tomita / Koji Ueda / Tomoyoshi Soga / Senthil K. Muthuswamy

    Communications Biology, Vol 5, Iss 1, Pp 1-

    2022  Band 10

    Abstract: A complex involving polarity protein SCRIB and the leucine amino acid transporter SLC7A5 promotes cell proliferation and tamoxifen resistance in estrogen receptor-positive breast cancer cells. ...

    Abstract A complex involving polarity protein SCRIB and the leucine amino acid transporter SLC7A5 promotes cell proliferation and tamoxifen resistance in estrogen receptor-positive breast cancer cells.
    Schlagwörter Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2022-05-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: TGF-β1 increases cellular invasion of colorectal neuroendocrine carcinoma cell line through partial epithelial-mesenchymal transition

    Norihiko Sasaki / Seiichi Shinji / Yuuki Shichi / Toshiyuki Ishiwata / Tomio Arai / Takeshi Yamada / Goro Takahashi / Ryo Ohta / Hiromichi Sonoda / Akihisa Matsuda / Takuma Iwai / Kohki Takeda / Kazuhide Yonaga / Koji Ueda / Sho Kuriyama / Toshimitsu Miyasaka / Hiroshi Yoshida

    Biochemistry and Biophysics Reports, Vol 30, Iss , Pp 101239- (2022)

    2022  

    Abstract: Epithelial–mesenchymal transition (EMT) plays a pivotal role in cancer progression and metastasis in many types of malignancies, including colorectal cancer. Although the importance of EMT is also considered in colorectal neuroendocrine carcinoma (NEC), ... ...

    Abstract Epithelial–mesenchymal transition (EMT) plays a pivotal role in cancer progression and metastasis in many types of malignancies, including colorectal cancer. Although the importance of EMT is also considered in colorectal neuroendocrine carcinoma (NEC), its regulatory mechanisms have not been elucidated. We recently established a human colorectal NEC cell line, SS-2. In this study, we aimed to clarify whether these cells were sensitive to transforming growth factor beta 1 (TGF-β1) and whether EMT could be induced through TGF-β1/Smad signaling, with the corresponding NEC cell-specific changes in invasiveness. In SS-2 cells, activation of TGF-β1 signaling, as indicated by phosphorylation of Smad2/3, was dose-dependent, demonstrating that SS-2 cells were responsive to TGF-β1. Analysis of EMT markers showed that mRNA levels changed with TGF-β1 treatment and that E-cadherin, an EMT marker, was expressed in cell-cell junctions even after TGF-β1 treatment. Invasion assays showed that TGF-β1-treated SS-2 cells invaded more rapidly than non-treated cells, and these cells demonstrated increased metalloproteinase activity and cell adhesion. Among integrins involved in cell-to-matrix adhesion, α2-integrin was exclusively upregulated in TGF-β1-treated SS-2 cells, but not in other colon cancer cell lines, and adhesion and invasion were inhibited by an anti-α2-integrin blocking antibody. Our findings suggest that α2-integrin may represent a novel therapeutic target for the metastasis of colorectal NEC cells.
    Schlagwörter Neuroendocrine carcinoma ; EMT ; TGF-β1 ; α2-integrin ; Adhesion ; Invasion ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Sprache Englisch
    Erscheinungsdatum 2022-07-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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