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Article ; Online: Hydrogen Peroxide Production of Individual Nanosecond Pulsed Discharges Submerged in Water of Elevated Conductivity.

Rataj, Raphael / Werneburg, Matthias / Below, Harald / Kolb, Juergen F

Chemphyschem : a European journal of chemical physics and physical chemistry

2023 Volume 24, Issue 19, Page(s) e202300143

Abstract: The production of hydrogen peroxide ( ... ...

Abstract	The production of hydrogen peroxide (H
Language	English
Publishing date	2023-08-10
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2025223-7
ISSN	1439-7641 ; 1439-4235
ISSN (online)	1439-7641
ISSN	1439-4235
DOI	10.1002/cphc.202300143
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields.

Wolff, Christina M / Kolb, Juergen F / Bekeschus, Sander

Biomedicines

2022 Volume 10, Issue 12

Abstract: In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake ... ...

Abstract	In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.
Language	English
Publishing date	2022-11-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10123084
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Article ; Online: Analysis of microstructural parameters of trabecular bone based on electrical impedance spectroscopy and deep neural networks.

Wei, Wenzuo / Shi, Fukun / Kolb, Juergen F

Bioelectrochemistry (Amsterdam, Netherlands)

2022 Volume 148, Page(s) 108232

Abstract: The potential of electrical impedance spectroscopy (EIS) was demonstrated for the investigation of microstructural properties of osseous tissue. Therefore, a deep neural network (DNN) was implemented for a sensitive assessment of different structural ... ...

Abstract	The potential of electrical impedance spectroscopy (EIS) was demonstrated for the investigation of microstructural properties of osseous tissue. Therefore, a deep neural network (DNN) was implemented for a sensitive assessment of different structural features that were derived on the basis of dielectric parameters, especially relative permittivities, recorded over a frequency range from 40 Hz to 5 MHz. The advantages of the developed method over conventional approaches, including equivalent circuit models (ECMs), linear regression and effective medium approximation (EMA), is the comprehensive quantification of bone morphologies by several microstructural parameters simultaneously, such as bone volume fraction (BV/TV), bone surface-volume-ratio (BS/BV), structure model index (SMI), trabecular number (Tb.N) and trabecular thickness (Tb.Th). The comparison of predictions of the DNN with an analysis of µCT-images confirmed a high accuracy for different microstructural parameters, which was indicated by corresponding Pearson correlation coefficients, especially for Tb.Th (r = 0.89) and BS/BV (r = 0.80). Concurrently, the approach was able to unambiguously discriminate anatomically similar bone regions (femoral head, greater trochanter and femoral neck) and therefore was capable to determine the morphological status of osseous tissue in detail. The classification was more discriminative than one based on classical linear discriminant analysis (LDA), due to the distinguishing features extracted by the DNN model. Accordingly, the method and model can serve as a potential tool for evaluating bone quality and bone status.
MeSH term(s)	Cancellous Bone/diagnostic imaging ; Dielectric Spectroscopy ; Femur/diagnostic imaging ; Neural Networks, Computer
Language	English
Publishing date	2022-08-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2022.108232
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Article ; Online: Enhanced resolution impedimetric analysis of cell responses from the distribution of relaxation times.

Shi, Fukun / Kolb, Juergen F

Biosensors & bioelectronics

2020 Volume 157, Page(s) 112149

Abstract: A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis ...

Abstract	A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis for the distribution of relaxation times (DRT). The DRT method provides high resolution for the identification of different polarization processes without a priori assumptions, as they are needed by more conventional approaches, such as an evaluation by equivalent circuit models. Accordingly, the physical properties of cells and their changes due to external stimuli can be uncovered and visualized and dispersion mechanisms introduced by Schwan et al. clearly identified. These are in particular relaxation processes at about 100 kHz that are associated with cell membrane characteristics and dominating respective changes of the distribution function for epithelial cell monolayers after exposure. A relatively moderate evolution at about 10 kHz may represent the polarization of extracellular matrices. Relaxation processes at around 1 MHz were suggested to be associated with intracellular changes. Conversely, the distribution of relaxation times can aid the optimization of the experimental design with respect to intended responses by an external stimulus.
MeSH term(s)	Algorithms ; Animals ; Biosensing Techniques/methods ; Cell Line ; Cell Membrane/chemistry ; Dielectric Spectroscopy/methods ; Electricity ; Epithelial Cells/chemistry ; Epithelial Cells/cytology ; Liver/chemistry ; Liver/cytology ; Normal Distribution ; Rats
Language	English
Publishing date	2020-03-30
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2020.112149
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Article: Enhanced resolution impedimetric analysis of cell responses from the distribution of relaxation times

Shi, Fukun / Kolb, Juergen F

Biosensors & bioelectronics. 2020 June 01, v. 157

2020

Abstract	A universal strategy for the sensitive investigation of cell responses to external stimuli, in particular nanosecond pulsed electric fields (nsPEFs), was developed based on electrical impedance spectroscopy (EIS) in combination with a multi-peak analysis for the distribution of relaxation times (DRT). The DRT method provides high resolution for the identification of different polarization processes without a priori assumptions, as they are needed by more conventional approaches, such as an evaluation by equivalent circuit models. Accordingly, the physical properties of cells and their changes due to external stimuli can be uncovered and visualized and dispersion mechanisms introduced by Schwan et al. clearly identified. These are in particular relaxation processes at about 100 kHz that are associated with cell membrane characteristics and dominating respective changes of the distribution function for epithelial cell monolayers after exposure. A relatively moderate evolution at about 10 kHz may represent the polarization of extracellular matrices. Relaxation processes at around 1 MHz were suggested to be associated with intracellular changes. Conversely, the distribution of relaxation times can aid the optimization of the experimental design with respect to intended responses by an external stimulus.
Keywords	biosensors ; cell membranes ; dielectric spectroscopy ; epithelial cells ; experimental design ; models ; physical properties ; pulsed electric fields
Language	English
Dates of publication	2020-0601
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2020.112149
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

Biomedicines : open access journal

2022 Volume 10, Issue , Nr. 12

Abstract	In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients. publishedVersion
Keywords	apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
Subject code	610
Language	English
Publisher	Basel : MDPI
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

Biomedicines : open access journal

2022 Volume 10, Issue , Nr. 12

Abstract	In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients. publishedVersion
Keywords	apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
Subject code	610
Language	English
Publisher	Basel : MDPI
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

Biomedicines : open access journal

2022 Volume 10, Issue , Nr. 12

Abstract	In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients. publishedVersion
Keywords	apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
Subject code	610
Language	English
Publisher	Basel : MDPI
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

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Article ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

Biomedicines : open access journal

2022 Volume 10, Issue , Nr. 12

Abstract	In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients. publishedVersion
Keywords	apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
Subject code	610
Language	English
Publisher	Basel : MDPI
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Wolff, Christina M. / Kolb, Juergen F. / Bekeschus, Sander

Biomedicines : open access journal

2022 Volume 10, Issue , Nr. 12

Abstract	In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients. publishedVersion
Keywords	apoptosis ; kINPen ; leukemia ; plasma medicine ; reactive oxygen species ; ROS ; 610 ; 570
Subject code	610
Language	English
Publisher	Basel : MDPI
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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