Article ; Online: SAF-A mutants disrupt chromatin structure through dominant negative effects on RNAs associated with chromatin.
Mammalian genome : official journal of the International Mammalian Genome Society
2021 Volume 33, Issue 2, Page(s) 366–381
Abstract: Here we provide a brief review of relevant background before presenting results of our investigation into the interplay between scaffold attachment factor A (SAF-A), chromatin-associated RNAs, and DNA condensation. SAF-A, also termed heterogenous nuclear ...
Abstract | Here we provide a brief review of relevant background before presenting results of our investigation into the interplay between scaffold attachment factor A (SAF-A), chromatin-associated RNAs, and DNA condensation. SAF-A, also termed heterogenous nuclear protein U (hnRNP U), is a ubiquitous nuclear scaffold protein that was implicated in XIST RNA localization to the inactive X-chromosome (Xi) but also reported to maintain open DNA packaging in euchromatin. Here we use several means to perturb SAF-A and examine potential impacts on the broad association of RNAs on euchromatin, and on chromatin compaction. SAF-A has an N-terminal DNA binding domain and C-terminal RNA binding domain, and a prominent model has been that the protein provides a single-molecule bridge between XIST RNA and chromatin. Here analysis of the impact of SAF-A on broad RNA-chromatin interactions indicate greater biological complexity. We focus on SAF-A's role with repeat-rich C |
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MeSH term(s) | Chromatin/genetics ; Euchromatin ; Nuclear Proteins/genetics ; RNA, Long Noncoding/genetics ; X Chromosome |
Chemical Substances | Chromatin ; Euchromatin ; Nuclear Proteins ; RNA, Long Noncoding |
Language | English |
Publishing date | 2021-12-02 |
Publishing country | United States |
Document type | Journal Article ; Research Support, N.I.H., Extramural |
ZDB-ID | 1058547-3 |
ISSN | 1432-1777 ; 0938-8990 |
ISSN (online) | 1432-1777 |
ISSN | 0938-8990 |
DOI | 10.1007/s00335-021-09935-8 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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