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Article ; Online: Human pluripotent stem cells: An alternative for 3D in vitro modelling of skin disease.

Khurana, Preeti / Kolundzic, Nikola / Flohr, Carsten / Ilic, Dusko

2021 Volume 30, Issue 11, Page(s) 1572–1587

Abstract: To effectively study the skin and its pathology, various platforms have been used to date, with in vitro 3D skin models being considered the future gold standard. These models have generally been engineered from primary cell lines. However, their short ... ...

Abstract	To effectively study the skin and its pathology, various platforms have been used to date, with in vitro 3D skin models being considered the future gold standard. These models have generally been engineered from primary cell lines. However, their short life span leading to the use of various donors, imposes issues with genetic variation. Human pluripotent stem cell (hPSC)-technology holds great prospects as an alternative to the use of primary cell lines to study the pathophysiology of human skin diseases. This is due to their potential to generate an unlimited number of genetically identical skin models that closely mimic the complexity of in vivo human skin. During the past decade, researchers have therefore started to use human embryonic and induced pluripotent stem cells (hESC/iPSC) to derive skin resident-like cells and components. These have subsequently been used to engineer hPSC-derived 3D skin models. In this review, we focus on the advantages, recent developments, and future perspectives in using hPSCs as an alternative cell source for modelling human skin diseases in vitro.
MeSH term(s)	Cell Culture Techniques, Three Dimensional ; Cell Line ; Humans ; Models, Biological ; Pluripotent Stem Cells ; Skin Diseases/pathology
Language	English
Publishing date	2021-06-04
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1130936-2
ISSN	1600-0625 ; 0906-6705
ISSN (online)	1600-0625
ISSN	0906-6705
DOI	10.1111/exd.14358
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Article ; Online: Epidermal Basement Membrane Substitutes for Bioengineering of Human Epidermal Equivalents.

Kolundzic, Nikola / Khurana, Preeti / Crumrine, Debra / Celli, Anna / Mauro, Theodora M / Ilic, Dusko

JID innovations : skin science from molecules to population health

2021 Volume 2, Issue 2, Page(s) 100083

Abstract: Epidermal basement membrane, a tightly packed network of extracellular matrix (ECM) components, is a source of physical, chemical, and biological factors required for the structural and functional homeostasis of the epidermis. Variations within the ECM ... ...

Abstract	Epidermal basement membrane, a tightly packed network of extracellular matrix (ECM) components, is a source of physical, chemical, and biological factors required for the structural and functional homeostasis of the epidermis. Variations within the ECM create distinct environments, which can affect the property of cells in the basal layer of the epidermis and subsequently affect keratinocyte differentiation and stratification. Very little attention has been paid to mimicking basement membrane in organotypic cultures. In this study, using parameters outlined in a consensus on the quality standard of organotypic models suitable for dermatological research, we have evaluated three basement membrane substitutes. We compared fibronectin with three complex three-dimensional matrices: Matrigel, decellularized dermal fibroblast‒produced and ‒assembled ECM, and a dry human amniotic membrane. Our results suggest that Matrigel is not a suitable substrate for human epidermal equivalent culture, whereas the two other complex three-dimensional substitutes, decellularized dermal fibroblast‒produced and ‒assembled ECM and dry human amniotic membrane, were superior to single layer fibronectin coating. Human epidermal equivalents cultured on either decellularized dermal fibroblast‒produced and ‒assembled ECM or on dry human amniotic membrane generated hemidesmosomes, whereas those on fibronectin did not. In addition, human epidermal equivalent cultured on decellularized dermal fibroblast‒produced and ‒assembled ECM and on dry human amniotic membrane can be maintained in culture 4 days longer than human epidermal equivalent cultured on fibronectin without compromising the barrier function.
Language	English
Publishing date	2021-12-07
Publishing country	Netherlands
Document type	Journal Article
ISSN	2667-0267
ISSN (online)	2667-0267
DOI	10.1016/j.xjidi.2021.100083
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Article ; Online: mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions.

Jeriha, Jakob / Kolundzic, Nikola / Khurana, Preeti / Perez-Dominguez, Andrea / Ilic, Dusko

Methods in molecular biology (Clifton, N.J.)

2020 Volume 2454, Page(s) 665–674

Abstract: Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast ... ...

Abstract	Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).
MeSH term(s)	Cell Differentiation/genetics ; Cellular Reprogramming/genetics ; Fibroblasts ; Induced Pluripotent Stem Cells ; RNA, Messenger/genetics ; Regenerative Medicine
Chemical Substances	RNA, Messenger
Language	English
Publishing date	2020-06-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/7651_2020_302
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Article ; Online: Human embryos from induced pluripotent stem cell-derived gametes: ethical and quality considerations.

Ilic, Dusko / Ogilvie, Caroline / Noli, Laila / Kolundzic, Nikola / Khalaf, Yacoub

Regenerative medicine

2017 Volume 12, Issue 6, Page(s) 681–691

Abstract: Protocols for successful differentiation of male and female gametes from induced pluripotent stem cells have been published. Although culture of precursor cells in a natural microenvironment remains necessary to achieve terminal differentiation, the ... ...

Abstract	Protocols for successful differentiation of male and female gametes from induced pluripotent stem cells have been published. Although culture of precursor cells in a natural microenvironment remains necessary to achieve terminal differentiation, the creation of human preimplantation embryos from induced pluripotent stem cell-derived gametes is technically feasible. Such embryos could provide a solution to the scarcity of human cleavage-stage embryos donated for research. Here, we discuss current technology, major research-related ethical concerns and propose the norms that would assure the quality and reliability of such embryos.
MeSH term(s)	Animals ; Cell Differentiation ; DNA Methylation ; Embryo, Mammalian/cytology ; Gametogenesis ; Gene Expression Profiling ; Humans ; Induced Pluripotent Stem Cells ; Mice ; MicroRNAs/metabolism ; Research Embryo Creation/ethics ; Research Embryo Creation/methods
Chemical Substances	MicroRNAs
Language	English
Publishing date	2017
Publishing country	England
Document type	Journal Article
ZDB-ID	2274500-2
ISSN	1746-076X ; 1746-0751
ISSN (online)	1746-076X
ISSN	1746-0751
DOI	10.2217/rme-2017-0052
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Zs.A 6627: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats.

Khurana, Preeti / Kolundzic, Nikola / Rogar, Marija / Hobbs, Carl / Wong, X F Colin C / Common, John E A / Ilic, Dusko / Liovic, Mirjana

Stem cell research

2020 Volume 45, Page(s) 101827

Abstract: We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on ... ...

Abstract	We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle keratinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics technology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.
MeSH term(s)	Cell Differentiation ; Filaggrin Proteins ; Humans ; Induced Pluripotent Stem Cells/metabolism ; Intermediate Filament Proteins/genetics ; Intermediate Filament Proteins/metabolism ; Male ; Stem Cell Research ; Tandem Repeat Sequences
Chemical Substances	FLG protein, human ; Filaggrin Proteins ; Intermediate Filament Proteins
Language	English
Publishing date	2020-04-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2393143-7
ISSN	1876-7753 ; 1873-5061
ISSN (online)	1876-7753
ISSN	1873-5061
DOI	10.1016/j.scr.2020.101827
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Article ; Online: Markers for Ca

Jeriha, Jakob / Kolundzic, Nikola / Khurana, Preeti / Gordon, Mitch / Celli, Anna / Mauro, Theodora M / Ilic, Dusko

Experimental dermatology

2020 Volume 29, Issue 12, Page(s) 1238–1242

Abstract: Differentiation of normal human keratinocytes (NHK) grown in vitro as a monolayer to confluency can be triggered with an acute increase in concentration of extracellular ... ...

Abstract	Differentiation of normal human keratinocytes (NHK) grown in vitro as a monolayer to confluency can be triggered with an acute increase in concentration of extracellular Ca
MeSH term(s)	Biomarkers/metabolism ; Calcium/pharmacology ; Cell Differentiation/drug effects ; Cells, Cultured ; Cornified Envelope Proline-Rich Proteins/genetics ; Epidermis/metabolism ; Gene Expression/drug effects ; Humans ; In Vitro Techniques ; Intercellular Signaling Peptides and Proteins/genetics ; Intercellular Signaling Peptides and Proteins/metabolism ; Keratinocytes/metabolism ; Keratinocytes/physiology ; Proteins/genetics ; RNA, Messenger/metabolism ; Zonula Occludens-1 Protein/metabolism
Chemical Substances	Biomarkers ; CDSN protein, human ; Cornified Envelope Proline-Rich Proteins ; Intercellular Signaling Peptides and Proteins ; KPRP protein, human ; LCE1C protein, human ; Proteins ; RNA, Messenger ; SPRR4 protein, human ; TJP1 protein, human ; Zonula Occludens-1 Protein ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2020-10-07
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1130936-2
ISSN	1600-0625 ; 0906-6705
ISSN (online)	1600-0625
ISSN	0906-6705
DOI	10.1111/exd.14199
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Article: Prospects for the Use of Induced Pluripotent Stem Cells in Animal Conservation and Environmental Protection.

Stanton, Morgan M / Tzatzalos, Evangeline / Donne, Matthew / Kolundzic, Nikola / Helgason, Ingvar / Ilic, Dusko

Stem cells translational medicine

2018 Volume 8, Issue 1, Page(s) 7–13

Abstract: Stem cells are unique cell populations able to copy themselves exactly as well as specialize into new cell types. Stem cells isolated from early stages of embryo development are pluripotent, i.e., can be differentiated into multiple different cell types. ...

Abstract	Stem cells are unique cell populations able to copy themselves exactly as well as specialize into new cell types. Stem cells isolated from early stages of embryo development are pluripotent, i.e., can be differentiated into multiple different cell types. In addition, scientists have found a way of reverting specialized cells from an adult into an embryonic-like state. These cells, that are as effective as cells isolated from early embryos, are termed induced pluripotent stem cells (iPSCs). The potency of iPSC technology is recently being employed by researchers aimed at helping wildlife and environmental conservation efforts. Ambitious attempts using iPSCs are being made to preserve endangered animals as well as reanimate extinct species, merging science fiction with reality. Other research to sustain natural resources and promote animal welfare are exploring iPSCs for laboratory grown animal products without harm to animals offering unorthodox options for creating meat, leather, and fur. There is great potential in iPSC technology and what can be achieved in consumerism, animal welfare, and environmental protection and conservation. Here, we discuss current research in the field of iPSCs and how these research groups are attempting to achieve their goals. Stem Cells Translational Medicine 2019;8:7-13.
MeSH term(s)	Animal Welfare ; Animals ; Biotechnology ; Conservation of Natural Resources ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism
Language	English
Publishing date	2018-09-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2642270-0
ISSN	2157-6580 ; 2157-6564
ISSN (online)	2157-6580
ISSN	2157-6564
DOI	10.1002/sctm.18-0047
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Article ; Online: Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype.

Kolundzic, Nikola / Khurana, Preeti / Hobbs, Carl / Rogar, Marija / Ropret, Sandra / Törmä, Hans / Ilic, Dusko / Liovic, Mirjana

Stem cell research

2019 Volume 37, Page(s) 101424

Abstract: We have generated MLi002-A, a new induced pluripotent stem cell (iPSC) line derived from keratinocytes of a skin punch biopsy of a female patient with the severe epidermolysis bullosa simplex Dowling-Meara phenotype and the keratin K5 E475G mutation. ... ...

Abstract	We have generated MLi002-A, a new induced pluripotent stem cell (iPSC) line derived from keratinocytes of a skin punch biopsy of a female patient with the severe epidermolysis bullosa simplex Dowling-Meara phenotype and the keratin K5 E475G mutation. Keratinocytes were reprogrammed using non-integrating Sendai virus vectors, and xeno-free culture conditions were used throughout. The characterization of MLi002-A cell line consisted of molecular karyotyping, mutation screening using restriction enzyme digestion and Sanger sequencing, and testing of the pluripotency and differentiation potentials by immunofluorescence of associated markers both in vitro and in vivo. This is the first iPSC model of EB Simplex.
MeSH term(s)	Cell Differentiation ; Cells, Cultured ; Cellular Reprogramming ; Epidermolysis Bullosa Simplex/genetics ; Epidermolysis Bullosa Simplex/pathology ; Female ; Heterozygote ; Humans ; Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/pathology ; Keratin-5/genetics ; Keratinocytes/metabolism ; Keratinocytes/pathology ; Mutation ; Phenotype
Chemical Substances	KRT5 protein, human ; Keratin-5
Language	English
Publishing date	2019-03-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2393143-7
ISSN	1876-7753 ; 1873-5061
ISSN (online)	1876-7753
ISSN	1873-5061
DOI	10.1016/j.scr.2019.101424
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Article ; Online: IGFBP-3/transferrin/transferrin receptor 1 complexes as principal mediators of IGFBP-3 delivery to colon cells in non-cancer and cancer tissues.

Miljuš, Goran / Malenković, Vesna / Đukanović, Blagoje / Kolundžić, Nikola / Nedić, Olgica

Experimental and molecular pathology

2015 Volume 98, Issue 3, Page(s) 431–438

Abstract: Purpose: The aim of this work was to study the involvement of IGFBP-3/Tf complexes in the pathology of colorectal carcinoma (CRC), quantify them, investigate their relation to iron concentration and binding to transferrin receptor (TfR) in colon tissue ( ...

Abstract	Purpose: The aim of this work was to study the involvement of IGFBP-3/Tf complexes in the pathology of colorectal carcinoma (CRC), quantify them, investigate their relation to iron concentration and binding to transferrin receptor (TfR) in colon tissue (non-cancer and cancer), and to assess the priority of this pathway for internalization of IGFBP-3. Methods: The presence of IGFBP-3/Tf complexes was analyzed in sera from healthy persons and patients with CRC, and in colon tissue by immunoblotting. Complexes were immunoprecipitated, quantified by immunoassay and structurally characterized by immunoblotting, lectin blotting and mass spectrometry. Complexes which interacted with colon cells were immunoprecipitated with anti-TfR1 antibody and studied. Colon tissue slides were subjected to immunohistochemical analysis. Results: The concentration of IGFBP-3/Tf complexes was three times lower in patients with CRC. They were increasingly carbonylated, sialylated, contained more Galβ4GlcNAc units, expressed altered charge density and increased affinity for metal ions. Immunoprecipitation experiments revealed more TfR1 on membranes than in cytosol of colon cells, also more in cancer than non-cancer tissue. TfR1 on membranes were less occupied with IGFBP-3/Tf complexes than in cytosol. Immunofluorescent staining indicated a remarkable degree of co-localization of IGFBP-3 and TfR1, evenly distributed in non-cancer tissue and both evenly and cell surface concentrated in cancer tissue. Conclusions: Increased expression of TfR1 on colon cell membranes in patients with CRC compensates for the reduced extracellular availability of IGFBP-3/Tf and TfR1 is the principal binding partner of extracellular IGFBP-3. IGFBP-3/Tf complexes in patients with CRC exhibit increased affinity for iron ions.
MeSH term(s)	Adult ; Aged ; Antigens, CD/metabolism ; Carcinoma/diagnosis ; Carcinoma/metabolism ; Case-Control Studies ; Cell Membrane/metabolism ; Colonic Neoplasms/diagnosis ; Colonic Neoplasms/metabolism ; Cytosol/metabolism ; Female ; Humans ; Insulin-Like Growth Factor Binding Protein 1/metabolism ; Iron/metabolism ; Male ; Middle Aged ; Protein Binding ; Protein Transport ; Receptors, Transferrin/metabolism ; Transferrin/metabolism
Chemical Substances	Antigens, CD ; CD71 antigen ; IGFBP1 protein, human ; Insulin-Like Growth Factor Binding Protein 1 ; Receptors, Transferrin ; Transferrin ; Iron (E1UOL152H7)
Language	English
Publishing date	2015-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	207655-x
ISSN	1096-0945 ; 0014-4800
ISSN (online)	1096-0945
ISSN	0014-4800
DOI	10.1016/j.yexmp.2015.03.035
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Article ; Online: Molecular forms of galectin-1 from human placenta and trophoblast cells

Ćujić Danica / Bojić-Trbojević Žanka / Kolundžić Nikola / Kadoya Toshihiko / Vićovac Ljiljana

Journal of the Serbian Chemical Society, Vol 80, Iss 2, Pp 159-

2015 Volume 169

Abstract: Galectin-1 (gal-1) is the best studied member of the galectin family of the human placenta assumed to play important roles in pregnancy. Standard isolation of gal-1 from human placenta using lactose extraction and affinity chromatography in the presence ... ...

Abstract	Galectin-1 (gal-1) is the best studied member of the galectin family of the human placenta assumed to play important roles in pregnancy. Standard isolation of gal-1 from human placenta using lactose extraction and affinity chromatography in the presence of a reducing agent, produced several known forms of gal-1, which were compared to the recombinant human gal-1 (rhgal-1) and oxidized recombinant human gal-1 (Ox-gal-1). Isolated placental gal-1 retained lectin binding activity, evidenced by hemagglutination and dot blot lectin assays. Characterization of the forms present by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS), based on hydrophilic interactions or immunorecognition, provided a sensitive tool for detection of the fine differences among the diverse molecular forms. The forms detected included previously established biologically active oxidized gal-1 and reduced gal-1, as well as some other currently uncharacterized (less investigate forms. [Projekat Ministarstva nauke Republike Srbije, br. 173004]
Keywords	galectin-1 ; placenta ; molecular form ; SELDI-TOF MS ; Chemistry ; QD1-999
Language	English
Publishing date	2015-01-01T00:00:00Z
Publisher	Serbian Chemical Society
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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