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  1. Article ; Online: Anti-skin-aging effects of human ceramides via collagen and fibrillin expression in dermal fibroblasts.

    Sugahara, Yurika / Komorisono, Masahiko / Kuwajima, Mami / Yoshikawa, Shuichi / Yoshida, Shiori / Maeda, Kazuhisa

    Bioscience, biotechnology, and biochemistry

    2022  Volume 86, Issue 9, Page(s) 1240–1246

    Abstract: Intercellular lipids comprise mainly ceramides, known to enhance the barrier function of the stratum corneum. However, the activities of ceramides inside the skin have not yet been fully elucidated. Here we examined how the human ceramide mixture (HC123) ...

    Abstract Intercellular lipids comprise mainly ceramides, known to enhance the barrier function of the stratum corneum. However, the activities of ceramides inside the skin have not yet been fully elucidated. Here we examined how the human ceramide mixture (HC123) functions in the dermis. We treated human skin fibroblasts with HC123-expressed fibroblast growth factor (FGF), transforming growth factor-β (TGF-β), collagen I, and fibrillin. We found that HC123 promoted the formation of collagen fibers and microfibrils (fibrillin) which affect the elasticity of the skin. We also confirmed that the gene expression of collagen and fibrillin is promoted via TGF-β and FGF2, respectively. We then investigated the permeability of HC123 for external use, in pursuit of evidence that HC123 may exert an anti-aging effect by penetrating into the dermis, activating fibroblasts, and promoting the production of collagen fibers and elastin-related microfibrils.
    MeSH term(s) Cells, Cultured ; Ceramides/metabolism ; Ceramides/pharmacology ; Collagen/metabolism ; Fibrillin-1/genetics ; Fibrillin-1/metabolism ; Fibrillin-2/metabolism ; Fibrillins/metabolism ; Fibroblasts ; Humans ; Transforming Growth Factor beta/metabolism
    Chemical Substances Ceramides ; Fibrillin-1 ; Fibrillin-2 ; Fibrillins ; Transforming Growth Factor beta ; Collagen (9007-34-5)
    Language English
    Publishing date 2022-07-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 1106450-x
    ISSN 1347-6947 ; 0916-8451
    ISSN (online) 1347-6947
    ISSN 0916-8451
    DOI 10.1093/bbb/zbac107
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4647: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Evaluation of intercellular lipid lamellae in the stratum corneum by polarized microscopy.

    Asada, Naoki / Morita, Ryo / Kamiji, Rikae / Kuwajima, Mami / Komorisono, Masahiko / Yamamura, Tatsuo / Ono, Naoaki / Kanaya, Shigehiko / Yoshikawa, Shuichi

    Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

    2021  Volume 28, Issue 3, Page(s) 391–401

    Abstract: Background: Intercellular lipids contain a lamellar structure that glows in polarized images. It could be expected that the intercellular lipid content be estimated from the luminance values calculated from polarized images of stratum corneum strips. ... ...

    Abstract Background: Intercellular lipids contain a lamellar structure that glows in polarized images. It could be expected that the intercellular lipid content be estimated from the luminance values calculated from polarized images of stratum corneum strips. Therefore, we attempted to develop a method for simple and rapid evaluation of the intercellular lipid content through a procedure. Herein, we demonstrated a relationship between the luminance value and the amount of ceramides, one of the main components of intercellular lipids.
    Materials and methods: The stratum corneum was collected from the forearm using slides with a pure rubber-based adhesive, which did not produce unnecessary luminescence under polarizing conditions. Images were analyzed using luminance indices. The positive secondary ion peak images were obtained using the time of flight-secondary ion mass spectrometry; the polarized and brightfield images were obtained using a polarized microscope. The ceramide and protein amount was measured by high-performance liquid chromatography and bicinchoninic acid protein assay after microscope imaging. Images and quantitative values were used to construct evaluation models based on a convolutional neural network (CNN).
    Results: There was a correlation between the highlighted areas of the polarized image to overlap with the area where ceramide-derived peak was detected. Evaluation of the CNN-based model of the polarized images predicted the amount of ceramides per unit of stratum corneum.
    Conclusion: The method proposed in the study enabled a large number of specimens to provide a simple, rapid, and efficient evaluation of the intercellular lipid content.
    MeSH term(s) Ceramides/analysis ; Chromatography, High Pressure Liquid ; Epidermis/metabolism ; Humans ; Microscopy
    Chemical Substances Ceramides
    Language English
    Publishing date 2021-11-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1229160-2
    ISSN 1600-0846 ; 0909-752X ; 1397-1344
    ISSN (online) 1600-0846
    ISSN 0909-752X ; 1397-1344
    DOI 10.1111/srt.13109
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4278: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Analysis of the rice mutant dwarf and gladius leaf 1. Aberrant katanin-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling.

    Komorisono, Masahiko / Ueguchi-Tanaka, Miyako / Aichi, Ikuko / Hasegawa, Yasuko / Ashikari, Motoyuki / Kitano, Hidemi / Matsuoka, Makoto / Sazuka, Takashi

    Plant physiology

    2005  Volume 138, Issue 4, Page(s) 1982–1993

    Abstract: Molecular genetic studies of plant dwarf mutants have indicated that gibberellin (GA) and brassinosteroid (BR) are two major factors that determine plant height; dwarf mutants that are caused by other defects are relatively rare, especially in monocot ... ...

    Abstract Molecular genetic studies of plant dwarf mutants have indicated that gibberellin (GA) and brassinosteroid (BR) are two major factors that determine plant height; dwarf mutants that are caused by other defects are relatively rare, especially in monocot species. Here, we report a rice (Oryza sativa) dwarf mutant, dwarf and gladius leaf 1 (dgl1), which exhibits only minimal response to GA and BR. In addition to the dwarf phenotype, dgl1 produces leaves with abnormally rounded tip regions. Positional cloning of DGL1 revealed that it encodes a 60-kD microtubule-severing katanin-like protein. The protein was found to be important in cell elongation and division, based on the observed cell phenotypes. GA biosynthetic genes are up-regulated in dgl1, but the expression of BR biosynthetic genes is not enhanced. The enhanced expression of GA biosynthetic genes in dgl1 is not caused by inappropriate GA signaling because the expression of these genes was repressed by GA3 treatment, and degradation of the rice DELLA protein SLR1 was triggered by GA3 in this mutant. Instead, aberrant microtubule organization caused by the loss of the microtubule-severing function of DGL1 may result in enhanced expression of GA biosynthetic genes in that enhanced expression was also observed in a BR-deficient mutant with aberrant microtubule organization. These results suggest that the function of DGL1 is important for cell and organ elongation in rice, and aberrant DGL1-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling.
    MeSH term(s) Chromosome Mapping ; Chromosomes, Plant ; Gene Expression Regulation, Plant ; Genes, Plant ; Gibberellins/biosynthesis ; Microtubules/genetics ; Microtubules/physiology ; Mutation ; Oryza/genetics ; Oryza/physiology ; Phenotype ; Phylogeny ; Plant Leaves/anatomy & histology ; Plant Leaves/physiology ; Plant Proteins/biosynthesis ; Signal Transduction/genetics ; Signal Transduction/physiology ; Steroids ; Up-Regulation
    Chemical Substances Gibberellins ; Plant Proteins ; Steroids
    Language English
    Publishing date 2005-07-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1104/pp.105.062968
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 1193: Show issues

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