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Article ; Online: The Ca2+ concentration impacts the cytokine production of mouse and human lymphoid cells and the polarization of human macrophages in vitro.

Eskiocak, Yusuf Cem / Ayyildiz, Zeynep Ozge / Gunalp, Sinem / Korkmaz, Asli / Helvaci, Derya Goksu / Dogan, Yavuz / Sag, Duygu / Wingender, Gerhard

PloS one

2023 Volume 18, Issue 2, Page(s) e0282037

Abstract: Various aspects of the in vitro culture conditions can impact the functional response of immune cells. For example, it was shown that a Ca2+ concentration of at least 1.5 mM during in vitro stimulation is needed for optimal cytokine production by ... ...

Abstract	Various aspects of the in vitro culture conditions can impact the functional response of immune cells. For example, it was shown that a Ca2+ concentration of at least 1.5 mM during in vitro stimulation is needed for optimal cytokine production by conventional αβ T cells. Here we extend these findings by showing that also unconventional T cells (invariant Natural Killer T cells, mucosal-associated invariant T cells, γδ T cells), as well as B cells, show an increased cytokine response following in vitro stimulation in the presence of elevated Ca2+ concentrations. This effect appeared more pronounced with mouse than with human lymphoid cells and did not influence their survival. A similarly increased cytokine response due to elevated Ca2+ levels was observed with primary human monocytes. In contrast, primary human monocyte-derived macrophages, either unpolarized (M0) or polarized into M1 or M2 macrophages, displayed increased cell death in the presence of elevated Ca2+ concentrations. Furthermore, elevated Ca2+ concentrations promoted phenotypic M1 differentiation by increasing M1 markers on M1 and M2 macrophages and decreasing M2 markers on M2 macrophages. However, the cytokine production of macrophages, again in contrast to the lymphoid cells, was unaltered by the Ca2+ concentration. In summary, our data demonstrate that the Ca2+ concentration during in vitro cultures is an important variable to be considered for functional experiments and that elevated Ca2+ levels can boost cytokine production by both mouse and human lymphoid cells.
MeSH term(s)	Humans ; Calcium/metabolism ; Macrophages/metabolism ; Cytokines/metabolism ; Monocytes/metabolism ; Cell Differentiation ; Lymphocytes/metabolism
Chemical Substances	Calcium (SY7Q814VUP) ; Cytokines
Language	English
Publishing date	2023-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0282037
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Article ; Online: Sudarshan Kriya Yoga Breathing and a Meditation Program for Burnout Among Physicians: A Randomized Clinical Trial.

Korkmaz, Asli / Bernhardsen, Guro Pauck / Cirit, Burcu / Koprucu Suzer, Gayem / Kayan, Hale / Biçmen, Hülya / Tahra, Muratcan / Suner, Asli / Lehto, Soili Marianne / Sag, Duygu / Saatcioglu, Fahri

JAMA network open

2024 Volume 7, Issue 1, Page(s) e2353978

Abstract: Importance: Physicians are exposed to high stress and strain that results in burnout, which affects them, their families, their patients, and the entire health care system; thus, there is an urgent need to develop methods to increase the resiliency of ... ...

Abstract	Importance: Physicians are exposed to high stress and strain that results in burnout, which affects them, their families, their patients, and the entire health care system; thus, there is an urgent need to develop methods to increase the resiliency of physicians. Sudarshan Kriya Yoga (SKY) is a comprehensive yoga breathing and meditation-based program that is a potential approach to mitigate physician burnout. Objective: To determine whether SKY can reduce psychological distress and improve wellness in physicians. Design, setting, and participants: This randomized clinical trial assessed the potential efficacy of SKY compared with a stress management education (SME) training as control. This study was conducted online from November 11, 2021, to March 14, 2022, and included physicians from Turkey, Germany, and Dubai. Both the SKY and the SME control groups received 1.5 hours of training for 3 consecutive days via a group video conference call. Participants were physicians willing to do some form of relaxation exercise everyday for 2 months. Exclusion criteria included presence of major illness and maintaining a regular mind-body program practice. Statistical analysis took place from March to November 2023. Interventions: Participants were randomly assigned 1:1 into 2 groups-the SKY group or the SME (control) group-using a computer algorithm. After the 3-day instruction period, the participants in the SKY group practiced for approximately 30 minutes per day on their own and participated in a weekly 1-hour, group-based online follow-up practice. After the 3-day instruction period, participants in the SME group reviewed and applied the notes from stress management education training at their initiative and had a weekly 1-hour group-based online follow-up session. Main outcomes and measures: The primary outcomes were stress and depression (measured by the 42-item Depression, Anxiety, and Stress Scale [DASS-42]) and insomnia measured by the Regensburg Insomnia Scale (RIS) with primary end point at 8 weeks. Secondary outcomes included anxiety (DASS-42); optimism (Life Orientation Test-Revised [LOT-R]); professional fulfillment, work exhaustion, interpersonal disengagement, and overall burnout (Professional Fulfillment Index [PFI]); and self-reported professional errors (Self-Reported Professional Error Questionnaire). Results: This study included 129 participants (SME, 63 participants [48.9%]; SKY, 66 participants [51.1%]; 115 females [89.2%]; 14 males [10.8%]; mean [SD] age, 46.2 [9.0] years). Compared with the SME control group, participants in the SKY group had significantly decreased stress on the DASS-42 at posttraining (difference, -6.8 points; 95% CI, -9.6 to -4.1 points; P = .006) and at postintervention (difference, -6.0 points; 95% CI, -8.8 to -3.3 points; P = .03), significantly decreased depression at posttraining (difference, -5.7 points; 95% CI, -8.6 to -2.8 points; P < .001) and postintervention (difference, -5.4 points; 95% CI, -8.3 to -2.5 points; P < .001), and significantly decreased anxiety at postintervention. In addition, there was a significant decrease in insomnia from baseline to postintervention in the SKY group (difference, -0.3 points; 95% CI, -2.3 to 1.7 points; P = .01). The SKY group also showed significantly increased professional fulfillment as well as significant decreases in work exhaustion, interpersonal disengagement, and burnout. There was no effect on self-reported medical errors. Conclusions and relevance: In this randomized clinical trial, physicians who regularly practiced SKY throughout a 2-month period experienced improvements in wellness and decreased burnout. These data suggest that SKY may be an effective, practical, and safe strategy to increase wellness and mitigate burnout in physicians. Trial registration: ClinicalTrials.gov Identifier: NCT05956470.
MeSH term(s)	Female ; Male ; Humans ; Middle Aged ; Meditation ; Yoga ; Sleep Initiation and Maintenance Disorders ; Burnout, Psychological ; Respiration
Language	English
Publishing date	2024-01-02
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article
ISSN	2574-3805
ISSN (online)	2574-3805
DOI	10.1001/jamanetworkopen.2023.53978
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Article ; Online: Effect of stimulation time on the expression of human macrophage polarization markers.

Unuvar Purcu, Duygu / Korkmaz, Asli / Gunalp, Sinem / Helvaci, Derya Goksu / Erdal, Yonca / Dogan, Yavuz / Suner, Asli / Wingender, Gerhard / Sag, Duygu

PloS one

2022 Volume 17, Issue 3, Page(s) e0265196

Abstract: Macrophages are highly plastic cells that can polarize into functionally distinct subsets in vivo and in vitro in response to environmental signals. The development of protocols to model macrophage polarization in vitro greatly contributes to our ... ...

Abstract	Macrophages are highly plastic cells that can polarize into functionally distinct subsets in vivo and in vitro in response to environmental signals. The development of protocols to model macrophage polarization in vitro greatly contributes to our understanding of macrophage biology. Macrophages are divided into two main groups: Pro-inflammatory M1 macrophages (classically activated) and anti-inflammatory M2 macrophages (alternatively activated), based on several key surface markers and the production of inflammatory mediators. However, the expression of these common macrophage polarization markers is greatly affected by the stimulation time used. Unfortunately, there is no consensus yet regarding the optimal stimulation times for particular macrophage polarization markers in in vitro experiments. This situation is problematic, (i) as analysing a particular marker at a suboptimal time point can lead to false-negative results, and (ii) as it clearly impedes the comparison of different studies. Using human monocyte-derived macrophages (MDMs) in vitro, we analysed how the expression of the main polarization markers for M1 (CD64, CD86, CXCL9, CXCL10, HLA-DR, IDO1, IL1β, IL12, TNF), M2a (CD200R, CD206, CCL17, CCL22, IL-10, TGM2), and M2c (CD163, IL-10, TGFβ) macrophages changes over time at mRNA and protein levels. Our data establish the most appropriate stimulation time for the analysis of the expression of human macrophage polarization markers in vitro. Providing such a reference guide will likely facilitate the investigation of macrophage polarization and its reproducibility.
MeSH term(s)	Biomarkers/metabolism ; Humans ; Interleukin-10/metabolism ; Macrophage Activation ; Macrophages/metabolism ; Reproducibility of Results
Chemical Substances	Biomarkers ; Interleukin-10 (130068-27-8)
Language	English
Publishing date	2022-03-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0265196
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Article ; Online: Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury.

Korkmaz, Asli / Kolankaya, Dürdane

Canadian journal of surgery. Journal canadien de chirurgie

2012 Volume 56, Issue 1, Page(s) 6–14

Abstract: Background: Nitric oxide (NO) seems to play an important role during renal ischemia/reperfusion (I/R) injury. We investigated whether rutin inhibits inducible nitric oxide synthase (iNOS) and reduces 3-nitrotyrosine (3-NT) formation in the kidneys of ... ...

Abstract	Background: Nitric oxide (NO) seems to play an important role during renal ischemia/reperfusion (I/R) injury. We investigated whether rutin inhibits inducible nitric oxide synthase (iNOS) and reduces 3-nitrotyrosine (3-NT) formation in the kidneys of rats during I/R. Methods: Wistar albino rats were nephrectomized unilaterally and, 2 weeks later, subjected to 45 minutes of left renal pedicle occlusion followed by 3 hours of reperfusion. We intraperitoneally administered L-N6-(1-iminoethyl)lysine (L-NIL; 3 mg/kg) for 30 minutes or rutin (1 g/kg) for 60 minutes before I/R. After reperfusion, kidney samples were taken for immunohistochemical analysis of iNOS and 3-NT. We measured plasma nitrite/nitrate and cyclic guanosine monophosphate (cGMP) to evaluate NO levels. Results: Ischemia/reperfusion caused plasma cGMP to increase significantly. Similarly, plasma nitrite/nitrate was elevated in the I/R group compared with the control group. Histochemical staining was positive for iNOS and 3-NT in the I/R group. Pretreatment with L-NIL or rutin significantly mitigated the elevation of plasma cGMP and nitrite/nitrate. These changes in biochemical parameters were also associated with changes in immunohistochemical appearance. Pretreatment with L-NIL or rutin significantly decreased the incidence and severity of iNOS and 3-NT formation in the kidney tissues. Conclusion: Our findings suggest that high activity of iNOS causes renal I/R injury, and that rutin exerts protective effects, probably by inhibiting iNOS.
MeSH term(s)	Animals ; Biomarkers/blood ; Cyclic GMP/blood ; Enzyme Inhibitors/administration & dosage ; Enzyme Inhibitors/pharmacology ; Immunohistochemistry ; Infusions, Parenteral ; Kidney/drug effects ; Kidney/enzymology ; Kidney/metabolism ; Lysine/analogs & derivatives ; Lysine/pharmacology ; Male ; Nephrectomy ; Nitrates/blood ; Nitric Oxide Synthase Type II/antagonists & inhibitors ; Nitric Oxide Synthase Type II/metabolism ; Nitrites/blood ; Protective Agents/administration & dosage ; Protective Agents/pharmacology ; Rats ; Rats, Wistar ; Reperfusion Injury/enzymology ; Reperfusion Injury/metabolism ; Reperfusion Injury/prevention & control ; Rutin/administration & dosage ; Rutin/pharmacology ; Tyrosine/analogs & derivatives ; Tyrosine/drug effects ; Tyrosine/metabolism
Chemical Substances	Biomarkers ; Enzyme Inhibitors ; N(6)-(1-iminoethyl)lysine ; Nitrates ; Nitrites ; Protective Agents ; 3-nitrotyrosine (3604-79-3) ; Tyrosine (42HK56048U) ; Rutin (5G06TVY3R7) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Cyclic GMP (H2D2X058MU) ; Lysine (K3Z4F929H6)
Language	English
Publishing date	2012-11-28
Publishing country	Canada
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	410651-9
ISSN	1488-2310 ; 0008-428X
ISSN (online)	1488-2310
ISSN	0008-428X
DOI	10.1503/cjs.004811
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Article ; Online: Bacterially activated B-cells drive T cell differentiation towards Tr1 through PD-1/PD-L1 expression.

Said, Sawsan Sudqi / Barut, Guliz Tuba / Mansur, Nesteren / Korkmaz, Asli / Sayi-Yazgan, Ayca

Molecular immunology

2018 Volume 96, Page(s) 48–60

Abstract: Regulatory B cells (Bregs) play a crucial role in immunological tolerance primarily through the production of IL-10 in many diseases including autoimmune disorders, allergy, infectious diseases, and cancer. To date, various Breg subsets with overlapping ... ...

Abstract	Regulatory B cells (Bregs) play a crucial role in immunological tolerance primarily through the production of IL-10 in many diseases including autoimmune disorders, allergy, infectious diseases, and cancer. To date, various Breg subsets with overlapping phenotypes have been identified. However, the roles of Bregs in Helicobacter infection are largely unknown. In the present study, we investigate the phenotype and function of Helicobacter -stimulated B cells. Our results demonstrate that Helicobacter felis -stimulated IL-10- producing B cells (Hf
MeSH term(s)	Animals ; B-Lymphocyte Subsets/immunology ; B7-H1 Antigen/immunology ; Cell Differentiation/immunology ; Helicobacter Infections/immunology ; Helicobacter felis ; Lymphocyte Activation/immunology ; Mice ; Mice, Inbred C57BL ; Programmed Cell Death 1 Receptor/immunology ; T-Lymphocyte Subsets/immunology
Chemical Substances	B7-H1 Antigen ; Cd274 protein, mouse ; Pdcd1 protein, mouse ; Programmed Cell Death 1 Receptor
Language	English
Publishing date	2018-02-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	424427-8
ISSN	1872-9142 ; 0161-5890
ISSN (online)	1872-9142
ISSN	0161-5890
DOI	10.1016/j.molimm.2018.02.010
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Article ; Online: Protective effect of rutin on the ischemia/reperfusion induced damage in rat kidney.

Korkmaz, Asli / Kolankaya, Dürdane

The Journal of surgical research

2010 Volume 164, Issue 2, Page(s) 309–315

Abstract: Reactive oxygen species (ROS) are suggested to participate in ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effect of rutin, a bioflavonoid, in I/R induced renal injury. Wistar albino rats were unilaterally ... ...

Abstract	Reactive oxygen species (ROS) are suggested to participate in ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effect of rutin, a bioflavonoid, in I/R induced renal injury. Wistar albino rats were unilaterally nephrectomized, and 2 wk later they were subjected to 45 min of left renal pedicle occlusion followed by 3h of reperfusion. Either rutin (1g/kg) or saline was administrated (i.p.) 1h prior to ischemia. At the end of the reperfusion period, kidney samples were taken for determination of renal malondialdehyde (MDA) and glutathione (GSH) levels, manganese-superoxide dismutase (MnSOD) activity and histological examination. Serum creatinine, blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) concentrations were measured for the evaluation of renal function. I/R caused a significant decrease in GSH level and MnSOD activity, which was accompanied by a significant increase in MDA level of kidney tissues. Similarly, serum BUN and creatinine levels, as well as LDH were elevated in the I/R group compared with the control group. Pretreatment of rats with rutin (1g/kg/ i.p.) significantly attenuated renal dysfunction, reduced elevated MDA levels, and restored the depleted MnSOD activity and GSH levels. These beneficial changes in the biochemical parameters were also associated with parallel changes in histopathological appearance. These findings suggest that ROS play a causal role in I/R induced renal injury, and that rutin exerts renal-protective effects, probably by inhibiting ROS and antioxidant activities.
MeSH term(s)	Animals ; Blood Urea Nitrogen ; Creatinine/metabolism ; Glutathione/metabolism ; Kidney Diseases/metabolism ; Kidney Diseases/pathology ; Kidney Diseases/prevention & control ; Kidney Diseases/surgery ; L-Lactate Dehydrogenase/blood ; Lipid Peroxidation ; Male ; Malondialdehyde/metabolism ; Necrosis ; Nephrectomy ; Oxidative Stress ; Rats ; Rats, Wistar ; Reperfusion ; Reperfusion Injury/prevention & control ; Rutin/therapeutic use ; Superoxide Dismutase/metabolism
Chemical Substances	Malondialdehyde (4Y8F71G49Q) ; Rutin (5G06TVY3R7) ; Creatinine (AYI8EX34EU) ; L-Lactate Dehydrogenase (EC 1.1.1.27) ; Superoxide Dismutase (EC 1.15.1.1) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2010-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80170-7
ISSN	1095-8673 ; 0022-4804
ISSN (online)	1095-8673
ISSN	0022-4804
DOI	10.1016/j.jss.2009.03.022
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Zs.A 178: Show issues

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Article ; Online: The protective effects of ascorbic acid against renal ischemia-reperfusion injury in male rats.

Korkmaz, Asli / Kolankaya, Dürdane

Renal failure

2009 Volume 31, Issue 1, Page(s) 36–43

Abstract: There is increasing evidence to suggest that toxic oxygen radicals play an essential role in the pathogenesis of ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effects of ascorbic acid (AA) in I/R-induced ... ...

Abstract	There is increasing evidence to suggest that toxic oxygen radicals play an essential role in the pathogenesis of ischemia/reperfusion (I/R) injury in the kidney. This study was designed to investigate the effects of ascorbic acid (AA) in I/R-induced renal injury in rats. Thirty two male Sprague-Dawley rats were divided equally into four groups: group 1 (control; dissection of the right renal pedicle without nephrectomy), group 2 (sham operated; unilateral nephrectomy), group 3 (I/R; unilateral nephrectomy + I/R); and group 4 (AA+I/R; unilateral nephrectomy and I/R treated with ascorbic acid, 250mg kg(-1) i.p., for one hour prior to ischemia). On the 15th day following nephrectomy, groups 3 and 4 were subjected to 45 min of renal pedicle occlusion followed by 3 h of reperfusion. At the end of the treatment period, kidney samples were taken for histological examination or determination of the renal malondialdehyde (MDA) and glutathione (GSH) levels. Serum creatinine, blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) concentrations were measured for the evaluation of renal function. I/R caused a significant decrease in GSH level, which was accompanied with a significant increase in MDA level of kidney tissues. Similarly, serum BUN and creatinine levels, as well as LDH, were elevated in the I/R group as compared to the control group. In group four, AA treatment reversed all the changes in these biochemical indices, as well as histopathological alterations normally induced by I/R. The findings imply that reactive oxygen species play a causal role in I/R-induced renal injury, and that AA exerts renoprotective effects, probably by radical scavenging and antioxidant activities.
MeSH term(s)	Acute Kidney Injury/etiology ; Acute Kidney Injury/pathology ; Acute Kidney Injury/prevention & control ; Animals ; Antioxidants/therapeutic use ; Ascorbic Acid/therapeutic use ; Blood Urea Nitrogen ; Glutathione/metabolism ; Lipid Peroxidation/physiology ; Male ; Malondialdehyde/metabolism ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury/drug therapy ; Reperfusion Injury/metabolism ; Reperfusion Injury/pathology
Chemical Substances	Antioxidants ; Malondialdehyde (4Y8F71G49Q) ; Glutathione (GAN16C9B8O) ; Ascorbic Acid (PQ6CK8PD0R)
Language	English
Publishing date	2009
Publishing country	England
Document type	Journal Article
ZDB-ID	632949-4
ISSN	1525-6049 ; 0886-022X
ISSN (online)	1525-6049
ISSN	0886-022X
DOI	10.1080/08860220802546271
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Zs.A 1331: Show issues

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Article ; Online: Anzer honey prevents N-ethylmaleimide-induced liver damage in rats.

Korkmaz, Asli / Kolankaya, Dürdane

Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie

2009 Volume 61, Issue 4, Page(s) 333–337

Abstract: N-ethylmaleimide (NEM) is a sulphydryl blocker which impairs the sulphydryl dependent antioxidant system (mainly glutathione) in the body by alkylating endogenous sulphydryls. This study was designed to investigate the effects of Anzer honey on NEM- ... ...

Abstract	N-ethylmaleimide (NEM) is a sulphydryl blocker which impairs the sulphydryl dependent antioxidant system (mainly glutathione) in the body by alkylating endogenous sulphydryls. This study was designed to investigate the effects of Anzer honey on NEM-induced liver injury in rats. Thirty female Wistar albino rats were divided equally into three groups. Group 1: control; Group 2: NEM; Group 3: Anzer honey+NEM. NEM (0.075mg kg(-1)) was given to both group 2 and 3 administered subcutaneously (s.c.) for 30 days. The animals in the Anzer honey+NEM group were treated with Anzer honey at a dose of 0.275g kg(-1), (p.o.) at 1h prior to every NEM injection. At the end of the 30 day treatment period, liver samples were taken for determination of the glutathione levels and histological examination. NEM treatment alone caused a significant reduction of the liver glutathione levels in group 2. Furthermore, NEM treatment caused congestion and mononuclear cell infiltration in the liver when compared to the control group. In group 3, Anzer honey treatment reversed all the changes in glutathione level, as well as histopathological alterations, normally induced by NEM. The findings imply that depletion of glutathione concentration plays a causal role in NEM-induced liver injury, and that the hepatoprotective effect of Anzer honey may be mediated through sulfhydryl-sensitive processes. They further imply that it may also possess antioxidant properties.
MeSH term(s)	Animals ; Antioxidants/analysis ; Antioxidants/therapeutic use ; Chemical and Drug Induced Liver Injury/etiology ; Chemical and Drug Induced Liver Injury/pathology ; Chemical and Drug Induced Liver Injury/prevention & control ; Ethylmaleimide/toxicity ; Female ; Glutathione/metabolism ; Honey/analysis ; Liver/drug effects ; Liver/metabolism ; Liver/pathology ; Oxidative Stress/drug effects ; Rats ; Rats, Wistar ; Reactive Oxygen Species/metabolism ; Sulfhydryl Reagents/toxicity
Chemical Substances	Antioxidants ; Reactive Oxygen Species ; Sulfhydryl Reagents ; Glutathione (GAN16C9B8O) ; Ethylmaleimide (O3C74ACM9V)
Language	English
Publishing date	2009-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1107321-4
ISSN	1618-1433 ; 0940-2993
ISSN (online)	1618-1433
ISSN	0940-2993
DOI	10.1016/j.etp.2008.07.005
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Zs.A 455: Show issues

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Article ; Online: Vitamin C coadministration augments bisphenol A, nonylphenol, and octylphenol induced oxidative damage on kidney of rats.

Korkmaz, Aslı / Aydoğan, Müfide / Kolankaya, Dürdane / Barlas, Nurhayat

Environmental toxicology

2011 Volume 26, Issue 4, Page(s) 325–337

Abstract: The aim of this study was to investigate whether bisphenol A (BPA), nonylphenol (NP), and octylphenol (OP) induce oxidative stress on the kidney tissue of male rats and whether coadministration of vitamin C, an antioxidant, can prevent any possible ... ...

Abstract	The aim of this study was to investigate whether bisphenol A (BPA), nonylphenol (NP), and octylphenol (OP) induce oxidative stress on the kidney tissue of male rats and whether coadministration of vitamin C, an antioxidant, can prevent any possible oxidative stress. The Wistar male rats were divided into seven groups, including control, BPA, NP, OP, BPA+C, NP + C, OP +C. BPA, NP, and OP (25 mg/kg/day) was administered alone; vitamin C (60 mg/kg/day) was administered along with BPA, OP, and NP to the rats for 50 days. There was a decrease in serum concentration of blood urea nitrogen (BUN) in NP and OP groups compared with control group. Vitamin C coadministration with BPA, NP, and OP did not produce significant increase in BUN concentration in BPA +C, NP+ C, and OP + C group as compared with BPA, NP, and OP groups, respectively. The lowest serum creatinine activity and the highest lactate dehydrogenase (LDH) activity was present in kidney of BPA+C, NP+C and OP+C groups compared with BPA, NP, and OP groups. The malondialdehyde (MDA) levels were significantly higher while glutathione (GSH) levels were lower in treatment groups than controls. Furthermore, an increase was observed in MDA levels whereas a decrease was observed in GSH levels in BPA+ C, NP + C, and OP+ C groups compared with BPA, NP, and OP groups, respectively. These finding are in accordance with immunohistochemical staining of MDA and GSH. Histopathological examination of the kidneys of rats in BPA, OP, NP, BPA+ C, NP + C, and OP+ C groups revealed necrotic lesions, congestion, and mononuclear cell infiltration. In conclusion BPA, NP, and OP might induce oxidative damage in kidney of rats. In addition, coadministration of vitamin C with BPA, NP, and OP to male rats augments this damage in the kidney of male rats.
MeSH term(s)	Animals ; Antioxidants/pharmacology ; Ascorbic Acid/pharmacology ; Benzhydryl Compounds ; Body Weight/drug effects ; Catalase/metabolism ; Creatinine/blood ; Endocrine Disruptors/toxicity ; Glutathione/metabolism ; Glutathione Peroxidase/metabolism ; Kidney/drug effects ; Kidney/metabolism ; Kidney/pathology ; Male ; Malondialdehyde/metabolism ; Organ Size/drug effects ; Oxidative Stress/drug effects ; Phenols/toxicity ; Rats ; Rats, Wistar ; Serum Albumin/metabolism ; Thiobarbituric Acid Reactive Substances/metabolism
Chemical Substances	Antioxidants ; Benzhydryl Compounds ; Endocrine Disruptors ; Phenols ; Serum Albumin ; Thiobarbituric Acid Reactive Substances ; octylphenol ; Malondialdehyde (4Y8F71G49Q) ; nonylphenol (79F6A2ILP5) ; Creatinine (AYI8EX34EU) ; Catalase (EC 1.11.1.6) ; Glutathione Peroxidase (EC 1.11.1.9) ; Glutathione (GAN16C9B8O) ; bisphenol A (MLT3645I99) ; Ascorbic Acid (PQ6CK8PD0R)
Language	English
Publishing date	2011-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	1463449-1
ISSN	1522-7278 ; 1520-4081
ISSN (online)	1522-7278
ISSN	1520-4081
DOI	10.1002/tox.20556
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Article: Vitamin C coadministration augments bisphenol A, nonylphenol, and octylphenol induced oxidative damage on kidney of rats

Korkmaz, Aslı / Aydoğan, Müfide / Kolankaya, Dürdane / Barlas, Nurhayat

Environmental toxicology. 2011 Aug., v. 26, no. 4

2011

Abstract	The aim of this study was to investigate whether bisphenol A (BPA), nonylphenol (NP), and octylphenol (OP) induce oxidative stress on the kidney tissue of male rats and whether coadministration of vitamin C, an antioxidant, can prevent any possible oxidative stress. The Wistar male rats were divided into seven groups, including control, BPA, NP, OP, BPA+C, NP + C, OP +C. BPA, NP, and OP (25 mg/kg/day) was administered alone; vitamin C (60 mg/kg/day) was administered along with BPA, OP, and NP to the rats for 50 days. There was a decrease in serum concentration of blood urea nitrogen (BUN) in NP and OP groups compared with control group. Vitamin C coadministration with BPA, NP, and OP did not produce significant increase in BUN concentration in BPA +C, NP+ C, and OP + C group as compared with BPA, NP, and OP groups, respectively. The lowest serum creatinine activity and the highest lactate dehydrogenase (LDH) activity was present in kidney of BPA+C, NP+C and OP+C groups compared with BPA, NP, and OP groups. The malondialdehyde (MDA) levels were significantly higher while glutathione (GSH) levels were lower in treatment groups than controls. Furthermore, an increase was observed in MDA levels whereas a decrease was observed in GSH levels in BPA+ C, NP + C, and OP+ C groups compared with BPA, NP, and OP groups, respectively. These finding are in accordance with immunohistochemical staining of MDA and GSH. Histopathological examination of the kidneys of rats in BPA, OP, NP, BPA+ C, NP + C, and OP+ C groups revealed necrotic lesions, congestion, and mononuclear cell infiltration. In conclusion BPA, NP, and OP might induce oxidative damage in kidney of rats. In addition, coadministration of vitamin C with BPA, NP, and OP to male rats augments this damage in the kidney of male rats.
Keywords	ascorbic acid ; bisphenol A ; blood ; creatinine ; glutathione ; histopathology ; immunohistochemistry ; kidneys ; lactate dehydrogenase ; malondialdehyde ; oxidative stress ; rats ; urea nitrogen
Language	English
Dates of publication	2011-08
Size	p. 325-337.
Publishing place	Wiley Subscription Services, Inc., A Wiley Company
Document type	Article
ZDB-ID	1463449-1
ISSN	1522-7278 ; 1520-4081
ISSN (online)	1522-7278
ISSN	1520-4081
DOI	10.1002/tox.20556
Database	NAL-Catalogue (AGRICOLA)

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