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  1. Article ; Online: Photo-Cross-Linked Probe-Modified Magnetic Particles for the Selective and Reliable Recovery of Nucleic Acids.

    Yajima, Shuto / Koto, Ayako / Koda, Maho / Sakamoto, Hiroaki / Takamura, Eiichiro / Suye, Shin-Ichiro

    ACS omega

    2022  Volume 7, Issue 15, Page(s) 12701–12706

    Abstract: Polymerase chain reaction (PCR) assays are used to diagnose various infectious diseases such as Coronavirus disease 2019 by detecting the nucleic acids of the pathogen. However, in practice, the yield of the extraction process and the inhibition of the ... ...

    Abstract Polymerase chain reaction (PCR) assays are used to diagnose various infectious diseases such as Coronavirus disease 2019 by detecting the nucleic acids of the pathogen. However, in practice, the yield of the extraction process and the inhibition of the reverse transcription reaction and PCR by foreign substances reduce the sensitivity and may yield false negative results. The sensitivity of the PCR test can be improved by using technologies that can reliably capture the target nucleic acid and remove foreign substances. In this study, we developed photo-cross-linkable probe-modified magnetic particles (PPMPs) for the sequence-specific recovery of target nucleic acids using photo-cross-linkable artificial nucleic acid probes and magnetic particles. Nucleic acid probes modified with photo-cross-linkable artificial nucleic acids can hybridize with the target nucleic acids in a sequence-specific manner and then securely capture the target nucleic acids by UV irradiation-mediated covalent bonding. Then the target nucleic acid is detected by trapping the target-bound probe on the surface of the magnetic particles and subjecting these collected magnetic particles to PCR. Recovery of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N gene pseudo-DNA (120 bp) was performed using PPMPs. We confirmed that the PPMPs captured the target consistently even after washes were done with denaturing agents and surfactants. Even in the presence of foreign DNA fragments, PPMPs were able to specifically recover the target DNA. This method allows for a more accurate detection by recovering only the target DNA for PCR. Hence, PPMPs can be successfully used for PCR-mediated detection of SARS-CoV-2 and other pathogens whose nucleic acid sequences are known.
    Language English
    Publishing date 2022-04-06
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.1c07012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Selective miR-21 detection technology based on photocrosslinkable artificial nucleic acid-modified magnetic particles and hybridization chain reaction.

    Watanabe, Yui / Yajima, Shuto / Koda, Maho / Kinjou, Ayumu / Koto, Ayako / Takamura, Eiichiro / Sakamoto, Hiroaki / Suye, Shin-Ichiro

    Biosensors & bioelectronics

    2023  Volume 247, Page(s) 115920

    Abstract: Recently, microRNA (miRNA) detection in blood has attracted attention as a new early detection technology for cancer. The extraction of target miRNA is a necessary preliminary step for detection; however, currently, most extraction methods extract all ... ...

    Abstract Recently, microRNA (miRNA) detection in blood has attracted attention as a new early detection technology for cancer. The extraction of target miRNA is a necessary preliminary step for detection; however, currently, most extraction methods extract all RNA from the blood, which limits the detection selectivity. Therefore, a method for the selective extraction and detection of target miRNA from blood is very important. In this study, we utilized photocrosslinkable artificial nucleic acids and the hybridization chain reaction (HCR) in an attempt to improve upon the current standard method RT-qPCR, which is hampered by problems with primer design and enzymatic amplification. By introducing photocrosslinkable artificial nucleic acids to oligonucleotide probes modified with magnetic particles with a sequence complementary to that of the target miRNA and irradiating them with light, covalent bonds were formed between the target miRNA and the oligonucleotide probes. These tight covalent bonds enabled the capture of miRNA in blood, and intensive washing ensured that only the target miRNA were extracted. After extraction, two types of DNA (H1 and H2) modified with fluorescent dyes were added and the fluorescence signals were amplified by the HCR in the presence of the target miRNA bound to the photocrosslinkable artificial nucleic acids, allowing for isothermal and enzyme-free miRNA detection. The novel method is suitable for selective miRNA detection in real blood samples. Because the reaction proceeds isothermally and no specialized equipment is used for washing, this detection technology is simple and selective and suitable for application to point-of-care technology using microfluidic devices.
    MeSH term(s) Nucleic Acids ; Oligonucleotide Probes ; Biosensing Techniques/methods ; Nucleic Acid Hybridization/methods ; MicroRNAs/genetics ; Magnetic Phenomena
    Chemical Substances Nucleic Acids ; Oligonucleotide Probes ; MicroRNAs
    Language English
    Publishing date 2023-12-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2023.115920
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article: DNA photo-cross-linking using a pyranocarbazole-modified oligodeoxynucleotide with a d-threoninol linker

    Fujimoto, Kenzo / Yamaguchi, Tsubasa / Inatsugi, Takahiro / Takamura, Masahiko / Ishimaru, Isao / Koto, Ayako / Nakamura, Shigetaka

    RSC advances. 2019 Sept. 27, v. 9, no. 53

    2019  

    Abstract: An alternative photo-cross-linker having a d-threoninol skeleton instead of the 2′-deoxyribose backbone in 3-cyanovinylcarbazole (CᴺⱽK) was investigated to improve the photoreactivity of photo-cross-linkers; the photo-cross-linking rate of 3- ... ...

    Abstract An alternative photo-cross-linker having a d-threoninol skeleton instead of the 2′-deoxyribose backbone in 3-cyanovinylcarbazole (CᴺⱽK) was investigated to improve the photoreactivity of photo-cross-linkers; the photo-cross-linking rate of 3-cyanovinylcarbazole with d-threoninol (CᴺⱽD) was found to be greater than that of CᴺⱽK. Therefore, in this study, a novel photo-cross-linker having pyranocarbazole (ᴾCX) and d-threoninol instead of the 2′-deoxyribose backbone in ᴾCX (ᴾCXD) was developed. The ᴾCXD in double-stranded DNA photo-cross-linked to a pyrimidine base at the −1 position of a complementary strand similar to ᴾCX. Furthermore, the photoreactivity of ᴾCXD was significantly higher than that of ᴾCX. The introduction of d-threoninol improved the reactivity of pyranocarbazole to cytosine, the use of ᴾCXD may extend the applicability of the photo-cross-linking reaction for DNA manipulation. In particular, this novel photo-cross-linker can contribute to the photochemical regulation of gene expression or biological events in a living cell.
    Keywords DNA ; cytosine ; gene expression regulation ; oligodeoxyribonucleotides ; photochemistry
    Language English
    Dates of publication 2019-0927
    Size p. 30693-30697.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/c9ra06145b
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: DNA photo-cross-linking using a pyranocarbazole-modified oligodeoxynucleotide with a d-threoninol linker.

    Fujimoto, Kenzo / Yamaguchi, Tsubasa / Inatsugi, Takahiro / Takamura, Masahiko / Ishimaru, Isao / Koto, Ayako / Nakamura, Shigetaka

    RSC advances

    2019  Volume 9, Issue 53, Page(s) 30693–30697

    Abstract: An alternative photo-cross-linker having a d-threoninol skeleton instead of the 2'-deoxyribose backbone in 3-cyanovinylcarbazole ( ...

    Abstract An alternative photo-cross-linker having a d-threoninol skeleton instead of the 2'-deoxyribose backbone in 3-cyanovinylcarbazole (
    Language English
    Publishing date 2019-09-27
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/c9ra06145b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Development of Biofuel Cell Using a Complex of Highly Oriented Immobilized His-Tagged Enzyme and Carbon Nanotube Surface Through a Pyrene Derivative.

    Sakamoto, Hiroaki / Koto, Ayako / Takamura, Ei-Ichiro / Asakawa, Hitoshi / Fukuma, Takeshi / Satomura, Takenori / Suye, Shin-Ichiro

    Journal of nanoscience and nanotechnology

    2019  Volume 19, Issue 6, Page(s) 3551–3557

    Abstract: For increasing the output of biofuel cells, increasing the cooperation between enzyme reaction and electron transfer on the electrode surface is essential. Highly oriented immobilization of enzymes onto a carbon nanotube (CNT) with a large specific ... ...

    Abstract For increasing the output of biofuel cells, increasing the cooperation between enzyme reaction and electron transfer on the electrode surface is essential. Highly oriented immobilization of enzymes onto a carbon nanotube (CNT) with a large specific surface area and excellent conductivity would increase the potential for their application as biosensors and biofuel cells, by utilizing the electron transfer between the electrode-molecular layer. In this study, we prepared a CNT-enzyme complex with highly oriented immobilization of enzyme onto the CNT surface. The complex showed excellent electrical characteristics, and could be used to develop biodevices that enable efficient electron transfer. Multi-walled carbon nanotubes (MWCNT) were dispersed by pyrene butyric acid
    MeSH term(s) Bioelectric Energy Sources ; Biosensing Techniques ; Electrodes ; Enzymes, Immobilized ; Glucose ; Nanotubes, Carbon ; Pyrenes
    Chemical Substances Enzymes, Immobilized ; Nanotubes, Carbon ; Pyrenes ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2019-02-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1533-4880
    ISSN 1533-4880
    DOI 10.1166/jnn.2019.16121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery.

    Sakamoto, Hiroaki / Uchii, Toshiki / Yamaguchi, Kayo / Koto, Ayako / Takamura, Ei-Ichiro / Satomura, Takenori / Sakuraba, Haruhiko / Ohshima, Toshihisa / Suye, Shin-Ichiro

    Biotechnology letters

    2015  Volume 37, Issue 7, Page(s) 1399–1404

    Abstract: Objectives: The life of biobatteries remains an issue due to loss of enzyme activity over time. In this study, we sought to develop a biobattery with a long life using a hyperthermophilic enzyme.: Results: We hypothesized that use of such ... ...

    Abstract Objectives: The life of biobatteries remains an issue due to loss of enzyme activity over time. In this study, we sought to develop a biobattery with a long life using a hyperthermophilic enzyme.
    Results: We hypothesized that use of such hyperthermophilic enzymes would allow for the biofuel cells to have a long battery life. Using pyrroloquinoline quinone-glucose dehydrogenase and the multicopper oxidase from Pyrobaculum aerophilum, we constructed an anode and cathode. The maximum output was 11 μW at 0.2 V, and the stability of the both electrode was maintained at 70 % after 14 days.
    Conclusion: The biofuel cells that use hyperthermophilic enzymes may prolong their life.
    MeSH term(s) Archaeal Proteins/metabolism ; Bioelectric Energy Sources ; Electrochemical Techniques/instrumentation ; Electrochemical Techniques/methods ; Electrodes ; Enzyme Stability ; Enzymes, Immobilized/metabolism ; Equipment Design ; Glucose 1-Dehydrogenase/metabolism ; Nanotubes, Carbon ; Oxidoreductases/metabolism ; PQQ Cofactor ; Pyrobaculum/enzymology
    Chemical Substances Archaeal Proteins ; Enzymes, Immobilized ; Nanotubes, Carbon ; PQQ Cofactor (72909-34-3) ; Oxidoreductases (EC 1.-) ; Glucose 1-Dehydrogenase (EC 1.1.1.47)
    Language English
    Publishing date 2015-07
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 423853-9
    ISSN 1573-6776 ; 0141-5492
    ISSN (online) 1573-6776
    ISSN 0141-5492
    DOI 10.1007/s10529-015-1819-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4564: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article: Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery

    Sakamoto, Hiroaki / Uchii, Toshiki / Yamaguchi, Kayo / Koto, Ayako / Takamura, Ei-ichiro / Satomura, Takenori / Sakuraba, Haruhiko / Ohshima, Toshihisa / Suye, Shin-ichiro

    Biotechnology letters. 2015 July, v. 37, no. 7

    2015  

    Abstract: OBJECTIVES: The life of biobatteries remains an issue due to loss of enzyme activity over time. In this study, we sought to develop a biobattery with a long life using a hyperthermophilic enzyme. RESULTS: We hypothesized that use of such ... ...

    Abstract OBJECTIVES: The life of biobatteries remains an issue due to loss of enzyme activity over time. In this study, we sought to develop a biobattery with a long life using a hyperthermophilic enzyme. RESULTS: We hypothesized that use of such hyperthermophilic enzymes would allow for the biofuel cells to have a long battery life. Using pyrroloquinoline quinone-glucose dehydrogenase and the multicopper oxidase from Pyrobaculum aerophilum, we constructed an anode and cathode. The maximum output was 11 μW at 0.2 V, and the stability of the both electrode was maintained at 70 % after 14 days. CONCLUSION: The biofuel cells that use hyperthermophilic enzymes may prolong their life.
    Keywords batteries ; electrodes ; enzyme activity ; enzymes ; microbial fuel cells
    Language English
    Dates of publication 2015-07
    Size p. 1399-1404.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 423853-9
    ISSN 1573-6776 ; 0141-5492
    ISSN (online) 1573-6776
    ISSN 0141-5492
    DOI 10.1007/s10529-015-1819-z
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4564: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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