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  1. AU="Kotp, Mohamed S"
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  1. Article ; Online: Sperm quality and testicular histopathology of Wistar albino male rats treated with hydroethanolic extract of

    El-Newary, Samah A / Aly, Mohamed S / Hameed, Amal R Abd El / Kotp, Mohamed S / Youssef, Abdelghany A / Ali, Naglaa A

    Pharmaceutical biology

    2022  Volume 60, Issue 1, Page(s) 282–293

    Abstract: Context: Cordia dichotoma: Objective: This study investigates the effect of : Materials and methods: 120 Wistar albino male rats were divided into four groups (: Results: After day 56, extract at 500 mg/kg significantly reduced sperm total ... ...

    Abstract Context: Cordia dichotoma
    Objective: This study investigates the effect of
    Materials and methods: 120 Wistar albino male rats were divided into four groups (
    Results: After day 56, extract at 500 mg/kg significantly reduced sperm total count, motility%, and alive%, to 47.60 ± 2.27 × 10
    Conclusions: Cordia
    MeSH term(s) Animals ; Anticholesteremic Agents/administration & dosage ; Anticholesteremic Agents/isolation & purification ; Anticholesteremic Agents/toxicity ; Cordia/chemistry ; Dose-Response Relationship, Drug ; Follicle Stimulating Hormone/metabolism ; Fruit ; Luteinizing Hormone/metabolism ; Male ; Plant Extracts/administration & dosage ; Plant Extracts/toxicity ; Rats ; Rats, Wistar ; Spermatozoa/drug effects ; Testis/drug effects ; Testis/pathology ; Testosterone/metabolism
    Chemical Substances Anticholesteremic Agents ; Plant Extracts ; Testosterone (3XMK78S47O) ; Luteinizing Hormone (9002-67-9) ; Follicle Stimulating Hormone (9002-68-0)
    Language English
    Publishing date 2022-02-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1440131-9
    ISSN 1744-5116 ; 1388-0209
    ISSN (online) 1744-5116
    ISSN 1388-0209
    DOI 10.1080/13880209.2021.2008455
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm.

    El-Seadawy, Islam E / Kotp, Mohamed S / El-Maaty, Amal M Abo / Fadl, Aya M / El-Sherbiny, Hossam R / Abdelnaby, Elshymaa A

    Tropical animal health and production

    2022  Volume 54, Issue 6, Page(s) 344

    Abstract: To increase rams' post-thaw semen quality following cryopreservation, this study used enriched Tris-based diluent with varying amounts of moringa leaf methanolic extract (MLME). The antioxidant activity, total phenolic, and total flavonoid content were ... ...

    Abstract To increase rams' post-thaw semen quality following cryopreservation, this study used enriched Tris-based diluent with varying amounts of moringa leaf methanolic extract (MLME). The antioxidant activity, total phenolic, and total flavonoid content were all assessed in MLME. The sperm of five healthy Awassi rams were collected, divided into 4 equal aliquots, and diluted [1:5; (v/v)] in Tris-citrate-glucose extender supplemented with 0.48, 0.56, and 0.64 mg MLME/ml or without MLME supplementation (control). The percentages of sperm total motility (STM, %), sperm progressive motility (SPM, %) and viability (V, %), abnormal morphology (AM, %), membrane functional integrity (MFI, %), and acrosome integrity (AI %) were measured. Malondialdehyde (MDA), nitric oxide (NO), ascorbic acid (AA), superoxide dismutase (SOD), glutathione peroxidase (GPx), total cholesterol (TC), low-density lipoproteins (LDL), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), zinc (Zn), and copper (Cu) were measured. The total phenolic gallic acid and flavonoid catechin (equivalent) contents were 19.78 mg/g and 11.94 mg/g, respectively. 2,2-Diphenyl-1-picrylhydrazyl (34.37 mM TE/g) and 2,2'-azino-bis/3-ethylbenzothiazoline-6-sulfonic acid (53.47 mM TE/g) were found in MLME. MLME had a 64.59 mM TE/g ferric-reducing power. In comparison to control, the addition of 0.64 mg/ml MLME to Tris-based extender resulted in the highest (P < 0.001) STM (55.22 ± 0.98), SPM (45.41 ± .70), SV (60.01 ± 1.05), MFI (75.23 ± 0.77), and AI (73.13 ± 0.72) and the lowest (P < 0.001) AM (21.34 ± 0.72) values. In comparison to the control, the addition of 0.56 mg/ml semen extender resulted in lower STM, SPM, SV, MFI, and AI with higher AM percentages. MDA (P = 0.03), NO (P = 0.012), CHO (P = 0.0001), and LDL (P = 0.004) were reduced by 0.64 mg/ml MLME, while AA (P = 0.017) and SOD (P = 0.0001) were elevated. In conclusion, the highest copper (P = 0.006) and lowest zinc concentrations in MLME (0.48 mg/ml extender) deteriorated the post-thaw semen quality, prompting us to suggest the addition of 0.64 mg MLME to rams' Tris-based semen extender.
    MeSH term(s) Alkaline Phosphatase ; Animals ; Antioxidants/pharmacology ; Ascorbic Acid/pharmacology ; Catechin/pharmacology ; Cholesterol ; Citrates/pharmacology ; Copper ; Cryopreservation/veterinary ; Cryoprotective Agents/pharmacology ; Dietary Supplements ; Gallic Acid/pharmacology ; Glucose/pharmacology ; Glutathione Peroxidase ; Lactate Dehydrogenases ; Lipoproteins, LDL/pharmacology ; Male ; Malondialdehyde ; Methanol/pharmacology ; Moringa ; Nitric Oxide ; Oxidants ; Plant Leaves ; Seeds ; Semen Analysis/veterinary ; Semen Preservation/methods ; Semen Preservation/veterinary ; Sheep ; Spermatozoa ; Sulfonic Acids/pharmacology ; Superoxide Dismutase ; Zinc/pharmacology
    Chemical Substances Antioxidants ; Citrates ; Cryoprotective Agents ; Lipoproteins, LDL ; Oxidants ; Sulfonic Acids ; Nitric Oxide (31C4KY9ESH) ; Malondialdehyde (4Y8F71G49Q) ; Gallic Acid (632XD903SP) ; Copper (789U1901C5) ; Catechin (8R1V1STN48) ; Cholesterol (97C5T2UQ7J) ; Lactate Dehydrogenases (EC 1.1.-) ; Glutathione Peroxidase (EC 1.11.1.9) ; Superoxide Dismutase (EC 1.15.1.1) ; Alkaline Phosphatase (EC 3.1.3.1) ; Glucose (IY9XDZ35W2) ; Zinc (J41CSQ7QDS) ; Ascorbic Acid (PQ6CK8PD0R) ; Methanol (Y4S76JWI15)
    Language English
    Publishing date 2022-10-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 603363-5
    ISSN 1573-7438 ; 0049-4747
    ISSN (online) 1573-7438
    ISSN 0049-4747
    DOI 10.1007/s11250-022-03344-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2159: Show issues Location:
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  3. Article ; Online: Sperm quality and testicular histopathology of Wistar albino male rats treated with hydroethanolic extract of Cordia dichotoma fruits

    El-Newary, Samah A. / Aly, Mohamed S. / Hameed, Amal R. Abd El / Kotp, Mohamed S. / Youssef, Abdelghany A. / Ali, Naglaa A.

    Pharmaceutical Biology. 2022 Dec. 31, v. 60, no. 1 p.282-293

    2022  

    Abstract: Cordia dichotoma Forst. (Boraginaceae) has potent pharmacological impact. Meanwhile, its effect on fertility is unclear. This study investigates the effect of Cordia fresh fruits hydroethanolic extract on fertility. 120 Wistar albino male rats were ... ...

    Abstract Cordia dichotoma Forst. (Boraginaceae) has potent pharmacological impact. Meanwhile, its effect on fertility is unclear. This study investigates the effect of Cordia fresh fruits hydroethanolic extract on fertility. 120 Wistar albino male rats were divided into four groups (n = 30). The first group was negative control, and the second, third, and fourth groups received 125, 250, and 500 mg extract/kg bodyweight for 56 days. After 56 days, Cordia force-feeding stopped, and all groups were kept under laboratory conditions for another month to study the recovering effect. After day 56, extract at 500 mg/kg significantly reduced sperm total count, motility%, and alive%, to 47.60 ± 2.27 × 10⁶ sperm/mL, 43.33% ± 1.49, and 63.67% ± 1.19, respectively, abnormalities% increased considerably (26.67% ± 0.54), compared to the negative control. Also, significant depletion on follicle-stimulating hormone (2.66 ± 0.21 mIU/L), luteinizing hormone (1.07 ± 0.06 mIU/L), and testosterone (2.69 ± 0.13 nmol/L) level was recorded, compared to the negative control. Cordia negative effect showed on histopathological studies of testes, prostate, and seminal vesicles. Fortunately, these adverse effects of Cordia recovered remarkably after stopping administration for one month. Cordia antifertility effect may be due to its hypocholesterolemic effect, where cholesterol, the steroid cycle precursor, was significantly reduced. This study can be incorporated in clinical research after being repeated on another small experimental animal, their offspring, and one large experimental animal, then going to a clinical study that we plan to do in the future.
    Keywords Cordia dichotoma ; albino ; antifertility effect ; biomedical research ; body weight ; cholesteremic effect ; cholesterol ; follicle-stimulating hormone ; histopathology ; laboratory animals ; luteinizing hormone ; males ; progeny ; sperm quality ; testes ; testosterone ; Seminal quality ; FSH and LH production ; histopathological studies
    Language English
    Dates of publication 2022-1231
    Size p. 282-293.
    Publishing place Taylor & Francis
    Document type Article ; Online
    ZDB-ID 1440131-9
    ISSN 1744-5116 ; 1388-0209
    ISSN (online) 1744-5116
    ISSN 1388-0209
    DOI 10.1080/13880209.2021.2008455
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1300: Show issues Location:
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  4. Article ; Online: The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm

    El-Seadawy, Islam E. / Kotp, Mohamed S. / El-Maaty, Amal M. Abo / Fadl, Aya M. / El-Sherbiny, Hossam R. / Abdelnaby, Elshymaa A.

    Trop Anim Health Prod. 2022 Dec., v. 54, no. 6 p.344-344

    2022  

    Abstract: To increase rams’ post-thaw semen quality following cryopreservation, this study used enriched Tris-based diluent with varying amounts of moringa leaf methanolic extract (MLME). The antioxidant activity, total phenolic, and total flavonoid content were ... ...

    Abstract To increase rams’ post-thaw semen quality following cryopreservation, this study used enriched Tris-based diluent with varying amounts of moringa leaf methanolic extract (MLME). The antioxidant activity, total phenolic, and total flavonoid content were all assessed in MLME. The sperm of five healthy Awassi rams were collected, divided into 4 equal aliquots, and diluted [1:5; (v/v)] in Tris-citrate-glucose extender supplemented with 0.48, 0.56, and 0.64 mg MLME/ml or without MLME supplementation (control). The percentages of sperm total motility (STM, %), sperm progressive motility (SPM, %) and viability (V, %), abnormal morphology (AM, %), membrane functional integrity (MFI, %), and acrosome integrity (AI %) were measured. Malondialdehyde (MDA), nitric oxide (NO), ascorbic acid (AA), superoxide dismutase (SOD), glutathione peroxidase (GPx), total cholesterol (TC), low-density lipoproteins (LDL), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), zinc (Zn), and copper (Cu) were measured. The total phenolic gallic acid and flavonoid catechin (equivalent) contents were 19.78 mg/g and 11.94 mg/g, respectively. 2,2-Diphenyl-1-picrylhydrazyl (34.37 mM TE/g) and 2,2′-azino-bis/3-ethylbenzothiazoline-6-sulfonic acid (53.47 mM TE/g) were found in MLME. MLME had a 64.59 mM TE/g ferric-reducing power. In comparison to control, the addition of 0.64 mg/ml MLME to Tris-based extender resulted in the highest (P < 0.001) STM (55.22 ± 0.98), SPM (45.41 ± .70), SV (60.01 ± 1.05), MFI (75.23 ± 0.77), and AI (73.13 ± 0.72) and the lowest (P < 0.001) AM (21.34 ± 0.72) values. In comparison to the control, the addition of 0.56 mg/ml semen extender resulted in lower STM, SPM, SV, MFI, and AI with higher AM percentages. MDA (P = 0.03), NO (P = 0.012), CHO (P = 0.0001), and LDL (P = 0.004) were reduced by 0.64 mg/ml MLME, while AA (P = 0.017) and SOD (P = 0.0001) were elevated. In conclusion, the highest copper (P = 0.006) and lowest zinc concentrations in MLME (0.48 mg/ml extender) deteriorated the post-thaw semen quality, prompting us to suggest the addition of 0.64 mg MLME to rams’ Tris-based semen extender.
    Keywords 2,2-diphenyl-1-picrylhydrazyl ; Awassi ; acrosome ; alkaline phosphatase ; antioxidant activity ; ascorbic acid ; catechin ; cholesterol ; copper ; cryopreservation ; freeze-thaw cycles ; gallic acid ; glutathione peroxidase ; lactate dehydrogenase ; leaves ; low density lipoprotein ; malondialdehyde ; nitric oxide ; rams ; semen extenders ; semen quality ; sperm quality ; superoxide dismutase ; viability ; zinc
    Language English
    Dates of publication 2022-12
    Size p. 344.
    Publishing place Springer Netherlands
    Document type Article ; Online
    ZDB-ID 603363-5
    ISSN 1573-7438 ; 0049-4747
    ISSN (online) 1573-7438
    ISSN 0049-4747
    DOI 10.1007/s11250-022-03344-y
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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