LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 66

Search options

  1. Article ; Online: The legacy of János Kovács: a lifelong devotion to advancing autophagy research.

    Kovács, Attila L / Lőw, Péter / Juhász, Gábor

    Autophagy

    2022  Volume 18, Issue 9, Page(s) 2017–2019

    MeSH term(s) Autophagy ; History, 20th Century ; Hungary
    Language English
    Publishing date 2022-06-23
    Publishing country United States
    Document type Editorial ; Historical Article
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.1080/15548627.2022.2091263
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6741: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: The application of traditional transmission electron microscopy for autophagy research in

    Kovács, Attila L

    Biophysics reports

    2015  Volume 1, Page(s) 99–105

    Abstract: Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature ... ...

    Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of
    Language English
    Publishing date 2015-10-23
    Publishing country China
    Document type Journal Article
    ISSN 2364-3439
    ISSN 2364-3439
    DOI 10.1007/s41048-015-0014-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: A simple method to estimate the number of autophagic elements by electron microscopic morphometry in real cellular dimensions.

    Kovács, Attila L

    BioMed research international

    2014  Volume 2014, Page(s) 578698

    Abstract: Autophagic elements typically appear as spherical bodies. During their life they undergo a series of changes (e.g., fusion, degradation of content, and swelling) which influence their size in a way that may be characteristic for cell type, stage of ... ...

    Abstract Autophagic elements typically appear as spherical bodies. During their life they undergo a series of changes (e.g., fusion, degradation of content, and swelling) which influence their size in a way that may be characteristic for cell type, stage of maturation, or various experimentally manipulated parameters. A simple and time efficient method is suggested here to use exactly calculated specific surface values and estimate average diameter and number of autophagic elements in real cellular dimensions. The method is based on the easiest morphometric determination of relative surface (surface density) and volume (volume density) data by electron microscopy. A series of data from real experimental samples of liver and exocrine pancreatic cells are offered to illustrate the potential of these measurements and calculations.
    MeSH term(s) Acinar Cells/ultrastructure ; Animals ; Autophagy ; Humans ; Microscopy, Electron, Transmission/methods ; Pancreas/ultrastructure
    Language English
    Publishing date 2014-07-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2014/578698
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis.

    Vedelek, Viktor / Kovács, Attila L / Juhász, Gábor / Alzyoud, Elham / Sinka, Rita

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 8422

    Abstract: The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila archipelago (ago) gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological ... ...

    Abstract The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila archipelago (ago) gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner such as cell proliferation, cellular differentiation, hypoxia-induced gene expression. Here we present a previously unknown tissue-specific role of Ago in spermatid differentiation. We identified a classical mutant of ago which is semi-lethal and male-sterile. During the characterization of ago function in testis, we found that ago plays role in spermatid development, following meiosis. We confirmed spermatogenesis defects by silencing ago by RNAi in testes. The ago mutants show multiple abnormalities in elongating and elongated spermatids, including aberration of the cyst morphology, malformed mitochondrial structures, and individualization defects. Additionally, we determined the subcellular localization of Ago protein with mCherry-Ago transgene in spermatids. Our findings highlight the potential roles of Ago in different cellular processes of spermatogenesis, like spermatid individualization, and regulation of mitochondrial morphology.
    MeSH term(s) Animals ; Cell Differentiation/genetics ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; F-Box Proteins/genetics ; F-Box Proteins/metabolism ; Genes, Tumor Suppressor ; Infertility, Male/genetics ; Male ; Mitochondria ; Mutation ; RNA Interference ; Spermatids/cytology ; Spermatogenesis/genetics ; Testis/cytology ; Testis/metabolism ; Ubiquitin-Protein Ligases/metabolism
    Chemical Substances Drosophila Proteins ; F-Box Proteins ; ago protein, Drosophila ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
    Language English
    Publishing date 2021-04-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-87656-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: A pioneer in the field of autophagy microscopy: Attila L Kovács.

    Kovács, Attila L / Klionsky, Daniel J

    Autophagy

    2013  Volume 9, Issue 12, Page(s) 1908–1921

    MeSH term(s) Animals ; Autophagy ; Caenorhabditis elegans/ultrastructure ; Drosophila melanogaster/ultrastructure ; Humans ; Hungary ; Microscopy, Electron, Transmission
    Language English
    Publishing date 2013-12
    Publishing country United States
    Document type Interview
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.4161/auto.26613
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6741: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Double membranes vs. lipid bilayers, and their significance for correct identification of macroautophagic structures.

    Eskelinen, Eeva-Liisa / Kovács, Attila L

    Autophagy

    2011  Volume 7, Issue 9, Page(s) 931–932

    MeSH term(s) Autophagy ; Microscopy, Electron ; Research Design
    Language English
    Publishing date 2011-09-01
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.4161/auto.7.9.16679
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6741: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Analysis of Drosophila Atg8 proteins reveals multiple lipidation-independent roles.

    Jipa, András / Vedelek, Viktor / Merényi, Zsolt / Ürmösi, Adél / Takáts, Szabolcs / Kovács, Attila L / Horváth, Gábor V / Sinka, Rita / Juhász, Gábor

    Autophagy

    2020  Volume 17, Issue 9, Page(s) 2565–2575

    Abstract: Yeast Atg8 and its homologs are involved in autophagosome biogenesis in all eukaryotes. These are the most widely used markers for autophagy thanks to the association of their lipidated forms with autophagic membranes. The Atg8 protein family expanded in ...

    Abstract Yeast Atg8 and its homologs are involved in autophagosome biogenesis in all eukaryotes. These are the most widely used markers for autophagy thanks to the association of their lipidated forms with autophagic membranes. The Atg8 protein family expanded in animals and plants, with most
    MeSH term(s) Animals ; Autophagy/genetics ; Autophagy-Related Protein 8 Family/genetics ; Autophagy-Related Protein 8 Family/metabolism ; Drosophila Proteins/genetics ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Microtubule-Associated Proteins/metabolism
    Chemical Substances Atg8a protein, Drosophila ; Autophagy-Related Protein 8 Family ; Drosophila Proteins ; Microtubule-Associated Proteins
    Language English
    Publishing date 2020-12-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2454135-7
    ISSN 1554-8635 ; 1554-8627
    ISSN (online) 1554-8635
    ISSN 1554-8627
    DOI 10.1080/15548627.2020.1856494
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6741: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.

    Laurinyecz, Barbara / Vedelek, Viktor / Kovács, Attila L / Szilasi, Kinga / Lipinszki, Zoltán / Slezák, Csilla / Darula, Zsuzsanna / Juhász, Gábor / Sinka, Rita

    PLoS genetics

    2019  Volume 15, Issue 2, Page(s) e1007987

    Abstract: Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and ... ...

    Abstract Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and organization is incompletely understood. The two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The molecular constituents and precise function of the paracrystalline material have not yet been revealed. Here we purified the paracrystalline material from mature sperm and identified by mass spectrometry Sperm-Leucylaminopeptidase (S-Lap) family members as important constituents of it. To study the function of S-Lap proteins we show the characterization of classical mutants and RNAi lines affecting of the S-Lap genes and the analysis of their mutant phenotypes. We show that the male sterile phenotype of the S-Lap mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives. Our work shows that S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative. Therefore, we propose that S-Lap proteins are important constituents of the paracrystalline material of Drosophila melanogaster sperm.
    MeSH term(s) Animals ; Animals, Genetically Modified ; Crystallization ; Drosophila Proteins/chemistry ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/enzymology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/physiology ; Fertility/genetics ; Fertility/physiology ; Genes, Insect ; Infertility, Male/enzymology ; Infertility, Male/genetics ; Leucyl Aminopeptidase/chemistry ; Leucyl Aminopeptidase/genetics ; Leucyl Aminopeptidase/metabolism ; Male ; Microscopy, Electron, Transmission ; Mitochondria/chemistry ; Mitochondria/enzymology ; Mitochondria/ultrastructure ; Mutation ; RNA Interference ; Spermatogenesis/genetics ; Spermatogenesis/physiology ; Spermatozoa/enzymology ; Spermatozoa/physiology ; Spermatozoa/ultrastructure
    Chemical Substances Drosophila Proteins ; Leucyl Aminopeptidase (EC 3.4.11.1)
    Language English
    Publishing date 2019-02-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1007987
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids.

    Cinege, Gyöngyi / Magyar, Lilla B / Kovács, Attila L / Lerner, Zita / Juhász, Gábor / Lukacsovich, David / Winterer, Jochen / Lukacsovich, Tamás / Hegedűs, Zoltán / Kurucz, Éva / Hultmark, Dan / Földy, Csaba / Andó, István

    Journal of innate immunity

    2021  Volume 14, Issue 4, Page(s) 335–354

    Abstract: Multinucleated giant hemocytes (MGHs) represent a novel type of blood cell in insects that participate in a highly efficient immune response against parasitoid wasps involving isolation and killing of the parasite. Previously, we showed that circulating ... ...

    Abstract Multinucleated giant hemocytes (MGHs) represent a novel type of blood cell in insects that participate in a highly efficient immune response against parasitoid wasps involving isolation and killing of the parasite. Previously, we showed that circulating MGHs have high motility and the interaction with the parasitoid rapidly triggers encapsulation. However, structural and molecular mechanisms behind these processes remained elusive. Here, we used detailed ultrastructural analysis and live cell imaging of MGHs to study encapsulation in Drosophila ananassae after parasitoid wasp infection. We found dynamic structural changes, mainly driven by the formation of diverse vesicular systems and newly developed complex intracytoplasmic membrane structures, and abundant generation of giant cell exosomes in MGHs. In addition, we used RNA sequencing to study the transcriptomic profile of MGHs and activated plasmatocytes 72 h after infection, as well as the uninduced blood cells. This revealed that differentiation of MGHs was accompanied by broad changes in gene expression. Consistent with the observed structural changes, transcripts related to vesicular function, cytoskeletal organization, and adhesion were enriched in MGHs. In addition, several orphan genes encoding for hemolysin-like proteins, pore-forming toxins of prokaryotic origin, were expressed at high level, which may be important for parasitoid elimination. Our results reveal coordinated molecular and structural changes in the course of MGH differentiation and parasitoid encapsulation, providing a mechanistic model for a powerful innate immune response.
    MeSH term(s) Animals ; Drosophila ; Hemocytes ; Host-Parasite Interactions ; Immunity, Innate ; Transcriptome ; Wasps/genetics
    Language English
    Publishing date 2021-12-03
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2454158-8
    ISSN 1662-8128 ; 1662-811X
    ISSN (online) 1662-8128
    ISSN 1662-811X
    DOI 10.1159/000520110
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6727: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Loss of Atg16 delays the alcohol-induced sedation response via regulation of Corazonin neuropeptide production in Drosophila.

    Varga, Kata / Nagy, Péter / Arsikin Csordás, Katarina / Kovács, Attila L / Hegedűs, Krisztina / Juhász, Gábor

    Scientific reports

    2016  Volume 6, Page(s) 34641

    Abstract: Autophagy defects lead to the buildup of damaged proteins and organelles, reduced survival during starvation and infections, hypersensitivity to stress and toxic substances, and progressive neurodegeneration. Here we show that, surprisingly, Drosophila ... ...

    Abstract Autophagy defects lead to the buildup of damaged proteins and organelles, reduced survival during starvation and infections, hypersensitivity to stress and toxic substances, and progressive neurodegeneration. Here we show that, surprisingly, Drosophila mutants lacking the core autophagy gene Atg16 are not only defective in autophagy but also exhibit increased resistance to the sedative effects of ethanol, unlike Atg7 or Atg3 null mutant flies. This mutant phenotype is rescued by the re-expression of Atg16 in Corazonin (Crz)-producing neurosecretory cells that are known to promote the sedation response during ethanol exposure, and RNAi knockdown of Atg16 specifically in these cells also delays the onset of ethanol-induced coma. We find that Atg16 and Crz colocalize within these neurosecretory cells, and both Crz protein and mRNA levels are decreased in Atg16 mutant flies. Thus, Atg16 promotes Crz production to ensure a proper organismal sedation response to ethanol.
    MeSH term(s) Animals ; Animals, Genetically Modified ; Autophagy/genetics ; Autophagy-Related Protein 7/deficiency ; Autophagy-Related Protein 7/genetics ; Autophagy-Related Proteins/deficiency ; Autophagy-Related Proteins/genetics ; Brain/cytology ; Brain/drug effects ; Brain/metabolism ; Drosophila Proteins/deficiency ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/drug effects ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Ethanol/pharmacology ; Gene Deletion ; Gene Expression Regulation ; Hypnotics and Sedatives/pharmacology ; Neuropeptides/genetics ; Neuropeptides/metabolism ; Neurosecretory Systems/cytology ; Neurosecretory Systems/drug effects ; Neurosecretory Systems/metabolism ; Protein Isoforms/deficiency ; Protein Isoforms/genetics ; Signal Transduction ; Time Factors
    Chemical Substances Atg16 protein, Drosophila ; Atg7 protein, Drosophila ; Autophagy-Related Proteins ; Crz protein, Drosophila ; Drosophila Proteins ; Hypnotics and Sedatives ; Neuropeptides ; Protein Isoforms ; Ethanol (3K9958V90M) ; Autophagy-Related Protein 7 (EC 6.2.1.45)
    Language English
    Publishing date 2016-10-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep34641
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top