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Artikel: Radiation-Induced Lymphopoenia and Treatment Outcome in Hereditary Breast Cancer Patients.

Argalácsová, Soňa / Křížová, Ľudmila / Matějů, Martin / Svobodová, Dominika / Vočka, Michal

2024 Band 69, Heft 3, Seite(n) 91–98

Abstract: Many breast cancer (BC) predisposition genes encode proteins involved in DNA damage repair (DDR). Identification of germline pathogenic va-riants (PV) in DDR genes raises the question whether their presence can influence the treatment outcomes and ... ...

Abstract	Many breast cancer (BC) predisposition genes encode proteins involved in DNA damage repair (DDR). Identification of germline pathogenic va-riants (PV) in DDR genes raises the question whether their presence can influence the treatment outcomes and potential radiation-induced toxicity in their carriers treated by adjuvant radiotherapy, which has not yet been answered conclusively. We retrospectively examined records of 213 BC patients treated by adjuvant radiotherapy, including 39 (18.3 %) BRCA1/2 PV carriers, 25 carriers (11.7 %) of PV in other breast cancer-predisposing genes, and 149 (70 %) non-carriers. Our goal was to examine 5-year disease-free survival (5y DFS) rates among the study groups and determine the impact of radiotherapy-induced lymphopoenia (RIL) on this outcome. While we found no significant difference in 5y DFS between non-carriers and carriers of BRCA mutations (86.4 % vs 78.4 % P = 0.24) or between non-carriers and other studied mutations (86.4 % vs 93.3 %; P = 0.27), respectively, we observed that the entire group of PV carriers had a significantly lower proportion of patients without RIL (P = 0.04) than the non-carriers. In contrast, subsequent analyses indicated a non-significant trend toward an increased 5y DFS in PV carriers with RIL. Our single-centre study indicated that the presence of PV in BC patients has an insignificant impact on DFS but can reduce the risk of RIL associated with adjuvant radiotherapy. It remains unclear whether this may result from the paradoxical activation of anti-tumour immunity in PV carriers with higher lymphocyte consumption resulting from higher immune effectiveness.
Mesh-Begriff(e)	Humans ; Female ; Breast Neoplasms/genetics ; Breast Neoplasms/radiotherapy ; BRCA1 Protein/genetics ; BRCA2 Protein/genetics ; Retrospective Studies ; Genetic Predisposition to Disease ; Treatment Outcome
Chemische Substanzen	BRCA1 protein, human ; BRCA1 Protein ; BRCA2 protein, human ; BRCA2 Protein
Sprache	Englisch
Erscheinungsdatum	2024-01-11
Erscheinungsland	Czech Republic
Dokumenttyp	Journal Article
ZDB-ID	419223-0
ISSN	0015-5500
ISSN	0015-5500
DOI	10.14712/fb2023069030091
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Microbiota as the unifying factor behind the hallmarks of cancer.

Benešová, Iva / Křížová, Ľudmila / Kverka, Miloslav

Journal of cancer research and clinical oncology

2023 Band 149, Heft 15, Seite(n) 14429–14450

Abstract: The human microbiota is a complex ecosystem that colonizes body surfaces and interacts with host organ systems, especially the immune system. Since the composition of this ecosystem depends on a variety of internal and external factors, each individual ... ...

Abstract	The human microbiota is a complex ecosystem that colonizes body surfaces and interacts with host organ systems, especially the immune system. Since the composition of this ecosystem depends on a variety of internal and external factors, each individual harbors a unique set of microbes. These differences in microbiota composition make individuals either more or less susceptible to various diseases, including cancer. Specific microbes are associated with cancer etiology and pathogenesis and several mechanisms of how they drive the typical hallmarks of cancer were recently identified. Although most microbes reside in the distal gut, they can influence cancer initiation and progression in distant tissues, as well as modulate the outcomes of established cancer therapies. Here, we describe the mechanisms by which microbes influence carcinogenesis and discuss their current and potential future applications in cancer diagnostics and management.
Sprache	Englisch
Erscheinungsdatum	2023-08-09
Erscheinungsland	Germany
Dokumenttyp	Journal Article ; Review
ZDB-ID	134792-5
ISSN	1432-1335 ; 0171-5216 ; 0084-5353 ; 0943-9382
ISSN (online)	1432-1335
ISSN	0171-5216 ; 0084-5353 ; 0943-9382
DOI	10.1007/s00432-023-05244-6
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Production of Bovine Equol-Enriched Milk: A Review

Křížová, Ludmila / Křešťáková, Veronika / Dadáková, Kateřina / Kašparovský, Tomáš

Animals. 2021 Mar. 08, v. 11, no. 3

2021

Abstract: Milk and dairy products are important sources of nutrients in the human diet because they contain a number of essential substances and other biologically active components. Many of these substances can be modified, and thus offer opportunities to use ... ...

Abstract	Milk and dairy products are important sources of nutrients in the human diet because they contain a number of essential substances and other biologically active components. Many of these substances can be modified, and thus offer opportunities to use milk and dairy products as functional food. Isoflavones are particularly important in human nutrition due to their diverse pharmacological and antioxidant properties. The clinical effectiveness of isoflavone-rich products is believed to be dependent on their ability to metabolize daidzein to equol, which may directly exert cancer preventive effects. However, only approximately 30–40% of humans are able to produce equol, while animals, in general, produce equol. Equol is the predominant product of bacterial metabolism of isoflavones and can be found in various amounts in some food of animal origin, especially in milk. Therefore, milk and dairy products can be considered to be sources of equol for humans who are not able to produce this metabolite. When the content of isoflavones in milk is to be modified, two groups of factors should be considered, i.e., dietary factors that include the source of isoflavones and the processing effects on feedstuffs and animal factors that include the intake of isoflavones, ruminal and postruminal changes, and the health and physiological status of animals. The approximate content of isoflavones in milk can be predicted using carry-over rates for different dietary sources or using a formula that describes the relationship between equol concentration in milk and formononetin intake. Processing and storage can affect the content and profile of isoflavones in milk and dairy products.
Schlagwörter	animal-based foods ; antioxidants ; cattle ; daidzein ; feeds ; formononetin ; functional foods ; human nutrition ; metabolism ; metabolites ; milk ; physiological state
Sprache	Englisch
Erscheinungsverlauf	2021-0308
Erscheinungsort	Multidisciplinary Digital Publishing Institute
Dokumenttyp	Artikel
Anmerkung	NAL-light
ISSN	2076-2615
DOI	10.3390/ani11030735
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: Alternative and Unconventional Feeds in Dairy Diets and Their Effect on Fatty Acid Profile and Health Properties of Milk Fat

Hadrová, Sylvie / Sedláková, Kateřina / Křížová, Ludmila / Malyugina, Svetlana

Animals. 2021 June 18, v. 11, no. 6

2021

Abstract: Milk fat is an important nutritional compound in the human diet. From the health point of view, some fatty acids (FAs), particularly long-chain PUFAs such as EPA and DHA, have been at the forefront of interest due to their antibacterial, antiviral, anti- ... ...

Abstract	Milk fat is an important nutritional compound in the human diet. From the health point of view, some fatty acids (FAs), particularly long-chain PUFAs such as EPA and DHA, have been at the forefront of interest due to their antibacterial, antiviral, anti-inflammatory, and anti-tumor properties, which play a positive role in the prevention of cardiovascular diseases (CVD), as well as linoleic and γ-linolenic acids, which play an important role in CVD treatment as essential components of phospholipids in the mitochondria of cell membranes. Thus, the modification of the FA profile—especially an increase in the concentration of polyunsaturated FAs and n-3 FAs in bovine milk fat—is desirable. The most effective way to achieve this goal is via dietary manipulations. The effects of various strategies in dairy nutrition have been thoroughly investigated; however, there are some alternative or unconventional feedstuffs that are often used for purposes other than basic feeding or modifying the fatty acid profiles of milk, such as tanniferous plants, herbs and spices, and algae. The use of these foods in dairy diets and their effects on milk fatty acid profile are reviewed in this article. The contents of selected individual FAs (atherogenic, rumenic, linoleic, α-linolenic, eicosapentaenoic, and docosahexaenoic acids) and their combinations; the contents of n3 and n6 FAs; n6/n3 ratios; and atherogenic, health-promoting and S/P indices were used as criteria for assessing the effect of these feeds on the health properties of milk fat.
Schlagwörter	docosahexaenoic acid ; eicosapentaenoic acid ; fatty acid composition ; feeds ; health promotion ; human nutrition ; milk ; milk fatty acids ; mitochondria ; phospholipids
Sprache	Englisch
Erscheinungsverlauf	2021-0618
Erscheinungsort	Multidisciplinary Digital Publishing Institute
Dokumenttyp	Artikel
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani11061817
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel: In sacco evaluation of ruminal degradability of isoflavones from full‐fat soybean and extracted soybean meal—A pilot study

Křížová, Ludmila / Němcová, Zuzana / Dadáková, Kateřina / Chrenková, Mária

Journal of animal physiology and animal nutrition. 2021 Sept., v. 105, no. 5

2021

Abstract: The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full‐fat soybean (GFFS) and solvent‐extracted soybean meal (SSBM) using the in sacco method. The experiment ...

Abstract	The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full‐fat soybean (GFFS) and solvent‐extracted soybean meal (SSBM) using the in sacco method. The experiment was carried out in three replications on ruminally cannulated sheep that were fed twice a day with a diet consisted of hay and supplemental mixture (6:4, DM basis). The nylon bags with 2 g feed samples ground to 2 mm were incubated in the rumen for 0, 2, 4, 8, 16 and 24 h. The effective degradability (ED) of DM, daidzein, genistein, glycitein and total isoflavones was calculated at outflow rate of 0.06 h. The ED of DM in GFFS was 77.8% and was higher than in SSBM being 71.8% (p < 0.001). The ED of daidzein (96.8%) and genistein (93.6%) was higher for SSBM compared with GFFS (93.9% and 92.8%, p < 0.001 and p = 0.003, respectively) while ED of glycitein was lower for SSBM than for GFFS (75.5 and 81.7%, respectively, p < 0.001). All isoflavones in the incubations were extensively degraded in the rumen, and regardless of dietary source, they were almost completely degraded after 16 h of incubation. Further, the disappearance patterns, that is the functions describing the time courses of the analyte disappearance, were assessed. The disappearance patterns of daidzein, genistein, glycitein and total isoflavones were similar and showed greater disappearance of mentioned isoflavones from SSBM compared to GFFS (p < 0.001 for daidzein, genistein and total isoflavones and p = 0.002 for glycitein). The study provides knowledge on the effect of processing on degradability of isoflavones in rumen that can be used to clarify the interrelationship between isoflavones and rumen microbiota.
Schlagwörter	animal nutrition ; animal physiology ; cannulas ; daidzein ; diet ; genistein ; glycitein ; hay ; nylon ; sheep ; soybean meal ; soybeans
Sprache	Englisch
Erscheinungsverlauf	2021-09
Umfang	p. 832-840.
Erscheinungsort	John Wiley & Sons, Ltd
Dokumenttyp	Artikel
Anmerkung	JOURNAL ARTICLE
ZDB-ID	232729-6
ISSN	0931-2439
ISSN	0931-2439
DOI	10.1111/jpn.13511
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: Feedborne Mycotoxins Beauvericin and Enniatins and Livestock Animals.

Křížová, Ludmila / Dadáková, Kateřina / Dvořáčková, Michaela / Kašparovský, Tomáš

Toxins

2021 Band 13, Heft 1

Abstract: Mycotoxins are secondary metabolites produced by several species of fungi, including ... ...

Abstract	Mycotoxins are secondary metabolites produced by several species of fungi, including the
Mesh-Begriff(e)	Animal Feed/toxicity ; Animals ; Depsipeptides/toxicity ; Edible Grain/toxicity ; Food Contamination/analysis ; Livestock ; Mycotoxins ; Terpenes
Chemische Substanzen	Depsipeptides ; Mycotoxins ; Terpenes ; enniatins ; fusaproliferin ; beauvericin (26S048LS2R)
Sprache	Englisch
Erscheinungsdatum	2021-01-05
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins13010032
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Alternative and Unconventional Feeds in Dairy Diets and Their Effect on Fatty Acid Profile and Health Properties of Milk Fat.

Hadrová, Sylvie / Sedláková, Kateřina / Křížová, Ludmila / Malyugina, Svetlana

Animals : an open access journal from MDPI

2021 Band 11, Heft 6

Abstract	Milk fat is an important nutritional compound in the human diet. From the health point of view, some fatty acids (FAs), particularly long-chain PUFAs such as EPA and DHA, have been at the forefront of interest due to their antibacterial, antiviral, anti-inflammatory, and anti-tumor properties, which play a positive role in the prevention of cardiovascular diseases (CVD), as well as linoleic and γ-linolenic acids, which play an important role in CVD treatment as essential components of phospholipids in the mitochondria of cell membranes. Thus, the modification of the FA profile-especially an increase in the concentration of polyunsaturated FAs and n-3 FAs in bovine milk fat-is desirable. The most effective way to achieve this goal is via dietary manipulations. The effects of various strategies in dairy nutrition have been thoroughly investigated; however, there are some alternative or unconventional feedstuffs that are often used for purposes other than basic feeding or modifying the fatty acid profiles of milk, such as tanniferous plants, herbs and spices, and algae. The use of these foods in dairy diets and their effects on milk fatty acid profile are reviewed in this article. The contents of selected individual FAs (atherogenic, rumenic, linoleic, α-linolenic, eicosapentaenoic, and docosahexaenoic acids) and their combinations; the contents of n3 and n6 FAs; n6/n3 ratios; and atherogenic, health-promoting and S/P indices were used as criteria for assessing the effect of these feeds on the health properties of milk fat.
Sprache	Englisch
Erscheinungsdatum	2021-06-18
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Review
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani11061817
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Production of Bovine Equol-Enriched Milk: A Review.

Křížová, Ludmila / Křešťáková, Veronika / Dadáková, Kateřina / Kašparovský, Tomáš

Animals : an open access journal from MDPI

2021 Band 11, Heft 3

Abstract	Milk and dairy products are important sources of nutrients in the human diet because they contain a number of essential substances and other biologically active components. Many of these substances can be modified, and thus offer opportunities to use milk and dairy products as functional food. Isoflavones are particularly important in human nutrition due to their diverse pharmacological and antioxidant properties. The clinical effectiveness of isoflavone-rich products is believed to be dependent on their ability to metabolize daidzein to equol, which may directly exert cancer preventive effects. However, only approximately 30-40% of humans are able to produce equol, while animals, in general, produce equol. Equol is the predominant product of bacterial metabolism of isoflavones and can be found in various amounts in some food of animal origin, especially in milk. Therefore, milk and dairy products can be considered to be sources of equol for humans who are not able to produce this metabolite. When the content of isoflavones in milk is to be modified, two groups of factors should be considered, i.e., dietary factors that include the source of isoflavones and the processing effects on feedstuffs and animal factors that include the intake of isoflavones, ruminal and postruminal changes, and the health and physiological status of animals. The approximate content of isoflavones in milk can be predicted using carry-over rates for different dietary sources or using a formula that describes the relationship between equol concentration in milk and formononetin intake. Processing and storage can affect the content and profile of isoflavones in milk and dairy products.
Sprache	Englisch
Erscheinungsdatum	2021-03-08
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article ; Review
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani11030735
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: In sacco evaluation of ruminal degradability of isoflavones from full-fat soybean and extracted soybean meal-A pilot study.

Křížová, Ludmila / Němcová, Zuzana / Dadáková, Kateřina / Chrenková, Mária

Journal of animal physiology and animal nutrition

2021 Band 105, Heft 5, Seite(n) 832–840

Abstract: The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full-fat soybean (GFFS) and solvent-extracted soybean meal (SSBM) using the in sacco method. The experiment ...

Abstract	The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full-fat soybean (GFFS) and solvent-extracted soybean meal (SSBM) using the in sacco method. The experiment was carried out in three replications on ruminally cannulated sheep that were fed twice a day with a diet consisted of hay and supplemental mixture (6:4, DM basis). The nylon bags with 2 g feed samples ground to 2 mm were incubated in the rumen for 0, 2, 4, 8, 16 and 24 h. The effective degradability (ED) of DM, daidzein, genistein, glycitein and total isoflavones was calculated at outflow rate of 0.06 h. The ED of DM in GFFS was 77.8% and was higher than in SSBM being 71.8% (p < 0.001). The ED of daidzein (96.8%) and genistein (93.6%) was higher for SSBM compared with GFFS (93.9% and 92.8%, p < 0.001 and p = 0.003, respectively) while ED of glycitein was lower for SSBM than for GFFS (75.5 and 81.7%, respectively, p < 0.001). All isoflavones in the incubations were extensively degraded in the rumen, and regardless of dietary source, they were almost completely degraded after 16 h of incubation. Further, the disappearance patterns, that is the functions describing the time courses of the analyte disappearance, were assessed. The disappearance patterns of daidzein, genistein, glycitein and total isoflavones were similar and showed greater disappearance of mentioned isoflavones from SSBM compared to GFFS (p < 0.001 for daidzein, genistein and total isoflavones and p = 0.002 for glycitein). The study provides knowledge on the effect of processing on degradability of isoflavones in rumen that can be used to clarify the interrelationship between isoflavones and rumen microbiota.
Mesh-Begriff(e)	Animals ; Diet/veterinary ; Isoflavones ; Pilot Projects ; Rumen ; Sheep ; Glycine max
Chemische Substanzen	Isoflavones
Sprache	Englisch
Erscheinungsdatum	2021-03-03
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	232729-6
ISSN	1439-0396 ; 0044-3565 ; 0931-2439
ISSN (online)	1439-0396
ISSN	0044-3565 ; 0931-2439
DOI	10.1111/jpn.13511
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Pembrolizumab as a first-line therapy in spinocellular esophageal cancer.

Křížová, Ludmila / Charvát, František / Petruželka, Luboš

Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti

2021 Band 34, Heft 1, Seite(n) 59–61

Abstract: Background: The treatment options in advanced stage of esophageal cancer are very limited. In recent years, a number of clinical trials have evaluated the effect of checkpoint inhibitors in this dia-gnosis.: Case: In our case report, we will ... ...

Titelübersetzung	Pembrolizumab v 1. linii terapie generalizovaného spinocelulárního karcinomu jícnu.
Abstract	Background: The treatment options in advanced stage of esophageal cancer are very limited. In recent years, a number of clinical trials have evaluated the effect of checkpoint inhibitors in this dia-gnosis. Case: In our case report, we will demonstrate the administration of pembrolizumab in first-line therapy in generalized squamous cell carcinoma of the esophagus with PD-L1 expression > 70%. Complete remission of the disease was achieved by this approach. Conclusion: Our case report shows the importance of investigating the expression of PD-L1 in advanced esophageal cancer. According to previous findings, immunotherapy, unlike chemotherapy, appears to have the potential to elicit a long-term response.
Mesh-Begriff(e)	Antibodies, Monoclonal, Humanized/therapeutic use ; Antineoplastic Agents, Immunological/therapeutic use ; B7-H1 Antigen ; Carcinoma, Squamous Cell/drug therapy ; Esophageal Neoplasms/drug therapy ; Humans ; Treatment Outcome
Chemische Substanzen	Antibodies, Monoclonal, Humanized ; Antineoplastic Agents, Immunological ; B7-H1 Antigen ; CD274 protein, human ; pembrolizumab (DPT0O3T46P)
Sprache	Englisch
Erscheinungsdatum	2021-03-03
Erscheinungsland	Czech Republic
Dokumenttyp	Case Reports ; Journal Article
ZDB-ID	1217739-8
ISSN	1802-5307 ; 0862-495X
ISSN (online)	1802-5307
ISSN	0862-495X
DOI	10.48095/ccko202159
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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