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  1. Article ; Online: Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca

    Carrisoza-Gaytan, Rolando / Kroll, Katharina T / Hiratsuka, Ken / Gupta, Navin R / Morizane, Ryuji / Lewis, Jennifer A / Satlin, Lisa M

    American journal of physiology. Cell physiology

    2023  Volume 324, Issue 3, Page(s) C757–C768

    Abstract: Kidney organoids cultured on adherent matrices in the presence of superfusate flow generate vascular networks and exhibit more mature podocyte and tubular compartments compared with static controls (Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, ...

    Abstract Kidney organoids cultured on adherent matrices in the presence of superfusate flow generate vascular networks and exhibit more mature podocyte and tubular compartments compared with static controls (Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, Miyoshi T, Mau D, Valerius MT, Ferrante T, Bonventre JV, Lewis JA, Morizane R.
    MeSH term(s) Animals ; Mice ; Calcium/metabolism ; Fura-2 ; Ion Channels/metabolism ; Kidney/metabolism ; Kidney Tubules/metabolism ; Calcium Signaling
    Chemical Substances Calcium (SY7Q814VUP) ; Fura-2 (TSN3DL106G) ; Ion Channels ; Piezo1 protein, mouse
    Language English
    Publishing date 2023-02-06
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 392098-7
    ISSN 1522-1563 ; 0363-6143
    ISSN (online) 1522-1563
    ISSN 0363-6143
    DOI 10.1152/ajpcell.00288.2022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery.

    Hiratsuka, Ken / Miyoshi, Tomoya / Kroll, Katharina T / Gupta, Navin R / Valerius, M Todd / Ferrante, Thomas / Yamashita, Michifumi / Lewis, Jennifer A / Morizane, Ryuji

    Science advances

    2022  Volume 8, Issue 38, Page(s) eabq0866

    Abstract: Organoids serve as a novel tool for disease modeling in three-dimensional multicellular contexts. Static organoids, however, lack the requisite biophysical microenvironment such as fluid flow, limiting their ability to faithfully recapitulate disease ... ...

    Abstract Organoids serve as a novel tool for disease modeling in three-dimensional multicellular contexts. Static organoids, however, lack the requisite biophysical microenvironment such as fluid flow, limiting their ability to faithfully recapitulate disease pathology. Here, we unite organoids with organ-on-a-chip technology to unravel disease pathology and develop therapies for autosomal recessive polycystic kidney disease.
    MeSH term(s) Drug Discovery ; Drugs, Investigational ; Humans ; Lab-On-A-Chip Devices ; Organoids/metabolism ; Polycystic Kidney, Autosomal Recessive/genetics ; Polycystic Kidney, Autosomal Recessive/metabolism ; Polycystic Kidney, Autosomal Recessive/pathology
    Chemical Substances Drugs, Investigational
    Language English
    Publishing date 2022-09-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abq0866
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Programming Cellular Alignment in Engineered Cardiac Tissue via Bioprinting Anisotropic Organ Building Blocks.

    Ahrens, John H / Uzel, Sebastien G M / Skylar-Scott, Mark / Mata, Mariana M / Lu, Aric / Kroll, Katharina T / Lewis, Jennifer A

    Advanced materials (Deerfield Beach, Fla.)

    2022  Volume 34, Issue 26, Page(s) e2200217

    Abstract: The ability to replicate the 3D myocardial architecture found in human hearts is a grand challenge. Here, the fabrication of aligned cardiac tissues via bioprinting anisotropic organ building blocks (aOBBs) composed of human induced pluripotent stem cell ...

    Abstract The ability to replicate the 3D myocardial architecture found in human hearts is a grand challenge. Here, the fabrication of aligned cardiac tissues via bioprinting anisotropic organ building blocks (aOBBs) composed of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) is reported. A bioink composed of contractile cardiac aOBBs is first generated and aligned cardiac tissue sheets with linear, spiral, and chevron features are printed. Next, aligned cardiac macrofilaments are printed, whose contractile force and conduction velocity increase over time and exceed the performance of spheroid-based cardiac tissues. Finally, the ability to spatially control the magnitude and direction of contractile force by printing cardiac sheets with different aOBB alignment is highlighted. This research opens new avenues to generating functional cardiac tissue with high cell density and complex cellular alignment.
    MeSH term(s) Bioprinting ; Humans ; Induced Pluripotent Stem Cells ; Myocardium ; Myocytes, Cardiac ; Printing, Three-Dimensional ; Tissue Engineering ; Tissue Scaffolds
    Language English
    Publishing date 2022-05-25
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1474949-X
    ISSN 1521-4095 ; 0935-9648
    ISSN (online) 1521-4095
    ISSN 0935-9648
    DOI 10.1002/adma.202200217
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Immune-infiltrated kidney organoid-on-chip model for assessing T cell bispecific antibodies.

    Kroll, Katharina T / Mata, Mariana M / Homan, Kimberly A / Micallef, Virginie / Carpy, Alejandro / Hiratsuka, Ken / Morizane, Ryuji / Moisan, Annie / Gubler, Marcel / Walz, Antje-Christine / Marrer-Berger, Estelle / Lewis, Jennifer A

    Proceedings of the National Academy of Sciences of the United States of America

    2023  Volume 120, Issue 35, Page(s) e2305322120

    Abstract: T cell bispecific antibodies (TCBs) are the focus of intense development for cancer immunotherapy. Recently, peptide-MHC (major histocompatibility complex)-targeted TCBs have emerged as a new class of biotherapeutics with improved specificity. These TCBs ...

    Abstract T cell bispecific antibodies (TCBs) are the focus of intense development for cancer immunotherapy. Recently, peptide-MHC (major histocompatibility complex)-targeted TCBs have emerged as a new class of biotherapeutics with improved specificity. These TCBs simultaneously bind to target peptides presented by the polymorphic, species-specific MHC encoded by the human leukocyte antigen (HLA) allele present on target cells and to the CD3 coreceptor expressed by human T lymphocytes. Unfortunately, traditional models for assessing their effects on human tissues often lack predictive capability, particularly for "on-target, off-tumor" interactions. Here, we report an immune-infiltrated, kidney organoid-on-chip model in which peripheral blood mononuclear cells (PBMCs) along with nontargeting (control) or targeting TCB-based tool compounds are circulated under flow. The target consists of the RMF peptide derived from the intracellular tumor antigen Wilms' tumor 1 (WT1) presented on HLA-A2 via a bivalent T cell receptor-like binding domain. Using our model, we measured TCB-mediated CD8
    MeSH term(s) Humans ; Antibodies, Bispecific ; HLA-A2 Antigen ; Leukocytes, Mononuclear ; Kidney ; Organoids
    Chemical Substances Antibodies, Bispecific ; HLA-A2 Antigen
    Language English
    Publishing date 2023-08-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2305322120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Acoustophoretic printing.

    Foresti, Daniele / Kroll, Katharina T / Amissah, Robert / Sillani, Francesco / Homan, Kimberly A / Poulikakos, Dimos / Lewis, Jennifer A

    Science advances

    2018  Volume 4, Issue 8, Page(s) eaat1659

    Abstract: Droplet-based printing methods are widely used in applications ranging from biological microarrays to additive manufacturing. However, common approaches, such as inkjet or electrohydrodynamic printing, are well suited only for materials with low ... ...

    Abstract Droplet-based printing methods are widely used in applications ranging from biological microarrays to additive manufacturing. However, common approaches, such as inkjet or electrohydrodynamic printing, are well suited only for materials with low viscosity or specific electromagnetic properties, respectively. While in-air acoustophoretic forces are material-independent, they are typically weak and have yet to be harnessed for printing materials. We introduce an acoustophoretic printing method that enables drop-on-demand patterning of a broad range of soft materials, including Newtonian fluids, whose viscosities span more than four orders of magnitude (0.5 to 25,000 mPa·s) and yield stress fluids (τ
    Language English
    Publishing date 2018-08-31
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.aat1659
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Flow-enhanced vascularization and maturation of kidney organoids in vitro.

    Homan, Kimberly A / Gupta, Navin / Kroll, Katharina T / Kolesky, David B / Skylar-Scott, Mark / Miyoshi, Tomoya / Mau, Donald / Valerius, M Todd / Ferrante, Thomas / Bonventre, Joseph V / Lewis, Jennifer A / Morizane, Ryuji

    Nature methods

    2019  Volume 16, Issue 3, Page(s) 255–262

    Abstract: Kidney organoids derived from human pluripotent stem cells have glomerular- and tubular-like compartments that are largely avascular and immature in static culture. Here we report an in vitro method for culturing kidney organoids under flow on ... ...

    Abstract Kidney organoids derived from human pluripotent stem cells have glomerular- and tubular-like compartments that are largely avascular and immature in static culture. Here we report an in vitro method for culturing kidney organoids under flow on millifluidic chips, which expands their endogenous pool of endothelial progenitor cells and generates vascular networks with perfusable lumens surrounded by mural cells. We found that vascularized kidney organoids cultured under flow had more mature podocyte and tubular compartments with enhanced cellular polarity and adult gene expression compared with that in static controls. Glomerular vascular development progressed through intermediate stages akin to those involved in the embryonic mammalian kidney's formation of capillary loops abutting foot processes. The association of vessels with these compartments was reduced after disruption of the endogenous VEGF gradient. The ability to induce substantial vascularization and morphological maturation of kidney organoids in vitro under flow opens new avenues for studies of kidney development, disease, and regeneration.
    MeSH term(s) Cells, Cultured ; Fibroblasts/cytology ; Human Umbilical Vein Endothelial Cells ; Humans ; In Vitro Techniques ; Kidney/blood supply ; Lab-On-A-Chip Devices ; Organ Culture Techniques ; Organoids/growth & development ; Printing, Three-Dimensional ; Tissue Engineering
    Language English
    Publishing date 2019-02-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/s41592-019-0325-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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