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  1. Book ; Online ; Thesis: Analysis of post-transcriptional regulation in Pseudomonas aeruginosa

    Krüger, Jonas [Verfasser]

    2022  

    Author's details vorgelegt von Jonas Krüger
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Leipzig ; Deutsche Nationalbibliothek
    Publishing place Frankfurt am Main
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Book ; Audio / Video ; Online: Forschungen zum Corona-Virus am Friedrich-Loeffler-Institut

    Krüger, Jonas

    2020  

    Abstract: Aufgrund der aktuellen Pandemie wird die Bedeutung von Forschungseinrichtungen wie dem Friedrich-Loeffler-Institut auf der Insel Riems erneut deutlich. Wir haben mit Prof. Dr. Dr. Mettenleiter, dem Präsidenten des Instituts, per Skype kommuniziert, um ... ...

    Abstract Aufgrund der aktuellen Pandemie wird die Bedeutung von Forschungseinrichtungen wie dem Friedrich-Loeffler-Institut auf der Insel Riems erneut deutlich. Wir haben mit Prof. Dr. Dr. Mettenleiter, dem Präsidenten des Instituts, per Skype kommuniziert, um mehr über die Arbeit der Einrichtung zu erfahren.
    Keywords Text ; ddc:630 ; Pressespiegel -- Friedrich-Loeffler-Institut -- Insel Riems -- Geschichte -- Forschung -- Coronavirus -- SARS-CoV-2 -- Covid-19 -- Tierversuch ; Frettchen ; covid19
    Language German
    Publishing date 2020-04-11
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: tRNA epitranscriptome determines pathogenicity of the opportunistic pathogen

    Krueger, Jonas / Preusse, Matthias / Oswaldo Gomez, Nicolas / Frommeyer, Yannick Noah / Doberenz, Sebastian / Lorenz, Anne / Kordes, Adrian / Grobe, Svenja / Müsken, Mathias / Depledge, Daniel P / Svensson, Sarah L / Weiss, Siegfried / Kaever, Volkhard / Pich, Andreas / Sharma, Cynthia M / Ignatova, Zoya / Häussler, Susanne

    Proceedings of the National Academy of Sciences of the United States of America

    2024  Volume 121, Issue 11, Page(s) e2312874121

    Abstract: The success of bacterial pathogens depends on the coordinated expression of virulence determinants. Regulatory circuits that drive pathogenesis are complex, multilayered, and incompletely understood. Here, we reveal that alterations in tRNA modifications ...

    Abstract The success of bacterial pathogens depends on the coordinated expression of virulence determinants. Regulatory circuits that drive pathogenesis are complex, multilayered, and incompletely understood. Here, we reveal that alterations in tRNA modifications define pathogenic phenotypes in the opportunistic pathogen
    MeSH term(s) Virulence/genetics ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/metabolism ; Proteomics ; RNA, Transfer/genetics ; RNA, Transfer/metabolism ; Anticodon ; Bacteria/metabolism
    Chemical Substances RNA, Transfer (9014-25-9) ; Anticodon
    Language English
    Publishing date 2024-03-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2312874121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Identification and Quantification of (t)RNA Modifications in Pseudomonas aeruginosa by Liquid Chromatography-Tandem Mass Spectrometry.

    Grobe, Svenja / Doberenz, Sebastian / Ferreira, Kevin / Krueger, Jonas / Brönstrup, Mark / Kaever, Volkhard / Häussler, Susanne

    Chembiochem : a European journal of chemical biology

    2019  Volume 20, Issue 11, Page(s) 1430–1437

    Abstract: Transfer RNA (tRNA) modifications impact the structure and function of tRNAs, thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined ... ...

    Abstract Transfer RNA (tRNA) modifications impact the structure and function of tRNAs, thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined role in adaptation to changing environments. In this study, we have explored tRNA modifications in P. aeruginosa through LC-MS/MS approaches. Neutral loss scanning (NLS) demonstrated the potential to identify previously unknown modifications, whereas multiple reaction monitoring (MRM) was able to detect modifications with high specificity and sensitivity. In this study, the MRM-based external calibration method allowed for quantification of the four canonical and 32 modified ribonucleosides, out of which 21 tRNA modifications were quantified in the total tRNA pool of P. aeruginosa PA14. We also purified the single tRNA isoacceptors tRNA-ArgUCU, tRNA-LeuCAA, and tRNA-TrpCCA and determined their specific modification patterns, both qualitatively and quantitatively. Deeper insights into the nature and dynamics of tRNA modifications in P. aeruginosa should pave the way for further studies on post-transcriptional gene regulation as a relatively unexplored molecular mechanism of controlling bacterial pathogenicity and mode of growth.
    MeSH term(s) Chromatography, Liquid/methods ; Pseudomonas aeruginosa/genetics ; RNA Processing, Post-Transcriptional ; RNA, Transfer/metabolism ; Ribonucleosides/metabolism ; Tandem Mass Spectrometry/methods
    Chemical Substances Ribonucleosides ; RNA, Transfer (9014-25-9)
    Language English
    Publishing date 2019-04-05
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.201800741
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  5. Article ; Online: Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa.

    Borgert, Sebastian Roman / Henke, Steffi / Witzgall, Florian / Schmelz, Stefan / Zur Lage, Susanne / Hotop, Sven-Kevin / Stephen, Steffi / Lübken, Dennis / Krüger, Jonas / Gomez, Nicolas Oswaldo / van Ham, Marco / Jänsch, Lothar / Kalesse, Markus / Pich, Andreas / Brönstrup, Mark / Häussler, Susanne / Blankenfeldt, Wulf

    Nature communications

    2022  Volume 13, Issue 1, Page(s) 7402

    Abstract: Pseudomonas aeruginosa is a major cause of nosocomial infections and also leads to severe exacerbations in cystic fibrosis or chronic obstructive pulmonary disease. Three intertwined quorum sensing systems control virulence of P. aeruginosa, with the rhl ...

    Abstract Pseudomonas aeruginosa is a major cause of nosocomial infections and also leads to severe exacerbations in cystic fibrosis or chronic obstructive pulmonary disease. Three intertwined quorum sensing systems control virulence of P. aeruginosa, with the rhl circuit playing the leading role in late and chronic infections. The majority of traits controlled by rhl transcription factor RhlR depend on PqsE, a dispensable thioesterase in Pseudomonas Quinolone Signal (PQS) biosynthesis that interferes with RhlR through an enigmatic mechanism likely involving direct interaction of both proteins. Here we show that PqsE and RhlR form a 2:2 protein complex that, together with RhlR agonist N-butanoyl-L-homoserine lactone (C4-HSL), solubilizes RhlR and thereby renders the otherwise insoluble transcription factor active. We determine crystal structures of the complex and identify residues essential for the interaction. To corroborate the chaperone-like activity of PqsE, we design stability-optimized variants of RhlR that bypass the need for C4-HSL and PqsE in activating PqsE/RhlR-controlled processes of P. aeruginosa. Together, our data provide insight into the unique regulatory role of PqsE and lay groundwork for developing new P. aeruginosa-specific pharmaceuticals.
    MeSH term(s) Virulence ; Pseudomonas aeruginosa/genetics ; Protein Folding ; Transcription Factors
    Chemical Substances N-butyrylhomoserine lactone ; Transcription Factors
    Language English
    Publishing date 2022-12-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-022-35030-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book: Das Geheimnis von El Escorial

    Krüger, Jonas Torsten

    (Carlsen ; 395)

    2006  

    Institution Real Monasterio de San Lorenzo de El Escorial
    Author's details Jonas Torsten Krüger
    Series title Carlsen ; 395
    Keywords Abenteuer ; Schulferien
    Language German
    Size 184 S, 19 cm
    Publisher Carlsen
    Publishing place Hamburg
    Document type Book
    ISBN 3551363951 ; 9783551363954
    Database Former special subject collection: coastal and deep sea fishing

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  7. Article ; Online: Identification and quantification of (t)RNA modifications in Pseudomonas aeruginosa by liquid chromatography-tandem mass spectrometry.

    Grobe, Svenja / Doberenz, Sebastian / Ferreira, Kevin / Krueger, Jonas / Brönstrup, Mark / Kaever, Volkhard / Häußler, Susanne

    Chembiochem : a European journal of chemical biology

    2019  

    Abstract: Transfer RNA (tRNA) modifications impact the structure and function of tRNAs thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined role ...

    Abstract Transfer RNA (tRNA) modifications impact the structure and function of tRNAs thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined role in the adaptation to changing environments. In this study, we explored tRNA modifications in P. aeruginosa using LC-MS/MS based approaches. Neutral Loss Scan (NLS) demonstrated the potential to identify previously unknown modifications, while Multiple Reaction Monitoring (MRM) can detect modifications with high specificity and sensitivity. In this study, the MRM-based external calibration method allowed for quantification of the 4 canonical and 32 modified ribonucleosides, of which 21 tRNA modifications were quantified in the total tRNA pool of P. aeruginosa PA14. We also purified the single tRNA isoacceptors tRNA-ArgUCU, tRNA-LeuCAA and tRNA-TrpCCA and determined, both qualitatively and quantitatively, their specific modification pattern. Deeper insights into the nature and dynamics of tRNA modifications in P. aeruginosa will pave the way for further studies on posttranscriptional gene regulation as a relatively unexplored molecular mechanism of controlling bacterial pathogenicity and life style.
    Keywords Mass spectrometry ; Pseudomonas aeruginosa ; tRNA ; tRNA modifications
    Subject code 420
    Language English
    Publishing date 2019-01-15
    Publisher Wiley-Blackwell
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  8. Article ; Online: Identification and quantification of (t)RNA modifications in Pseudomonas aeruginosa by liquid chromatography-tandem mass spectrometry.

    Grobe, Svenja / Doberenz, Sebastian / Ferreira, Kevin / Krueger, Jonas / Brönstrup, Mark / Kaever, Volkhard / Häußler, Susanne

    Chembiochem : a European journal of chemical biology

    2019  

    Abstract: Transfer RNA (tRNA) modifications impact the structure and function of tRNAs thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined role ...

    Abstract Transfer RNA (tRNA) modifications impact the structure and function of tRNAs thus affecting the efficiency and fidelity of translation. In the opportunistic pathogen Pseudomonas aeruginosa translational regulation plays an important but less defined role in the adaptation to changing environments. In this study, we explored tRNA modifications in P. aeruginosa using LC-MS/MS based approaches. Neutral Loss Scan (NLS) demonstrated the potential to identify previously unknown modifications, while Multiple Reaction Monitoring (MRM) can detect modifications with high specificity and sensitivity. In this study, the MRM-based external calibration method allowed for quantification of the 4 canonical and 32 modified ribonucleosides, of which 21 tRNA modifications were quantified in the total tRNA pool of P. aeruginosa PA14. We also purified the single tRNA isoacceptors tRNA-ArgUCU, tRNA-LeuCAA and tRNA-TrpCCA and determined, both qualitatively and quantitatively, their specific modification pattern. Deeper insights into the nature and dynamics of tRNA modifications in P. aeruginosa will pave the way for further studies on posttranscriptional gene regulation as a relatively unexplored molecular mechanism of controlling bacterial pathogenicity and life style.
    Keywords Mass spectrometry ; Pseudomonas aeruginosa ; tRNA ; tRNA modifications
    Subject code 420
    Language English
    Publishing date 2019-01-15
    Publisher Wiley-Blackwell
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Book ; Online: Arbeitsmarktspiegel

    Vom Berge, Philipp / Copestake, Silvina / Eberle, Johanna / Kaimer, Steffen / Klosterhuber, Wolfram / Krüger, Jonas / Trenkle, Simon / Zakrocki, Veronika

    Entwicklungen nach Einführung des Mindestlohns (Ausgabe 1)

    (IAB-Forschungsbericht ; 2016/1)

    2016  

    Abstract: On January 1, 2015 a statutory minimum wage was introduced in Germany. The Arbeitsmarktspiegel shows several important developments of the German labour market since its introduction. While overall employment in Germany continues to show a positive trend, ...

    Author's details Philipp vom Berge (IAB), Steffen Kaimer (IAB), Silvina Copestake (IAB), Johanna Eberle (IAB), Wolfram Klosterhuber (IAB), Jonas Krüger (IAB), Simon Trenkle (IAB), Veronika Zakrocki (IAB)
    Series title IAB-Forschungsbericht ; 2016/1
    Abstract On January 1, 2015 a statutory minimum wage was introduced in Germany. The Arbeitsmarktspiegel shows several important developments of the German labour market since its introduction. While overall employment in Germany continues to show a positive trend, the number of exclusively marginal part-time employees drops markedly at the turn of the year. This effect is strongest in industries and regions with a low average wage level. A little more than half of the decrease can be explained by persons changing directly into exclusively employment subject to social security. Yet, the data also show an increased amount of exits out of the labour market or at least out of the group of persons covered by the data. Transitions into unemployment play a rather secondary role in explaining the decline of marginal employment. The number of employees receiving additional benefits according to Social Code Book II slightly decreases after the introduction of the minimum wage. For this group, both the transitions into employment without benefit receipt as well as into exclusive benefit receipt are slightly increased.

    Zum 1. Januar 2015 wurde in Deutschland ein gesetzlicher Mindestlohn eingeführt. Der Arbeitsmarktspiegel beschreibt vor dem Hintergrund dieser Einführung wichtige Entwicklungen auf dem deutschen Arbeitsmarkt. Während die Gesamtbeschäftigung in Deutschland weiterhin einem positiven Trend folgt, sank die Zahl der ausschließlich geringfügig Beschäftigten zum Jahreswechsel 2014/2015 deutlich. Dieser Rückgang war in Branchen und Regionen mit niedrigem durchschnittlichem Lohnniveau am höchsten. Etwas mehr als die Hälfte des Rückgangs ist dadurch zu erklären, dass die betroffenen Personen direkt in ausschließlich sozialversicherungspflichtige Beschäftigung übergingen. Daneben verließen aber auch vermehrt Personen den Arbeitsmarkt bzw. tauchen in den Daten nicht länger auf. Übergänge in reine Arbeitslosigkeit spielen bei der Erklärung insgesamt eine eher untergeordnete Rolle. Die Zahl der Beschäftigten mit Bezug von SGB-II-Leistungen geht nach der Einführung des Mindestlohns leicht zurück. In geringem Umfang steigen zum Jahreswechsel die Übergänge sowohl in reine Beschäftigung ohne weiteren Leistungsbezug als auch in reinen Leistungsbezug unter Wegfall der Beschäftigung.
    Keywords Mindestlohn ; Beschäftigungseffekt ; Deutschland
    Language German ; English
    Size 1 Online-Ressource (circa 64 Seiten), Illustrationen
    Publisher Institut für Arbeitsmarkt- und Berufsforschung der Bundesagentur für Arbeit
    Publishing place Nürnberg
    Document type Book ; Online
    Note Zusammenfassung in englischer Sprache
    Database ECONomics Information System

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  10. Book ; Online: Arbeitsmarktspiegel

    Vom Berge, Philipp vom / Copestake, Silvina / Croxton, Daniela / Eberle, Johanna / Kaimer, Steffen / Klosterhuber, Wolfram / Krüger, Jonas

    Entwicklungen nach Einführung des Mindestlohns (Ausgabe 2)

    (IAB-Forschungsbericht ; 2016/12)

    2016  

    Abstract: Die zweite Ausgabe des Arbeitsmarktspiegels beschreibt anhand aktueller Daten bis einschließlich Januar 2016 wichtige Entwicklungen auf dem deutschen Arbeitsmarkt vor dem Hintergrund der Einführung des gesetzlichen Mindestlohns in Deutschland. Die ... ...

    Author's details Philipp vom Berge, Steffen Kaimer, Silvina Copestake, Daniela Croxton, Johanna Eberle, Wolfram Klosterhuber, Jonas Krüger
    Series title IAB-Forschungsbericht ; 2016/12
    Abstract Die zweite Ausgabe des Arbeitsmarktspiegels beschreibt anhand aktueller Daten bis einschließlich Januar 2016 wichtige Entwicklungen auf dem deutschen Arbeitsmarkt vor dem Hintergrund der Einführung des gesetzlichen Mindestlohns in Deutschland. Die Gesamtbeschäftigung steigt in 2015 weiter an. Die Zahl der ausschließlich geringfügig Beschäftigten, welche zum Jahreswechsel 2014/2015 saisonbereinigt um etwa 93.000 Personen sank, weist bis Ende des dritten Quartals 2015 eine rückläufige Tendenz auf. In den Wintermonaten könnte der Tiefpunkt dieser negativen Entwicklung erreicht sein. Ausgabe 2 des Arbeitsmarktspiegels zeigt erstmals, dass der Großteil der Übergänge aus ausschließlich geringfügiger in ausschließlich sozialversicherungspflichtige Beschäftigung im selben Betrieb erfolgt. Für beschäftigte SGB-II-Leistungsbezieher zeigt sich, dass nach Abklingen der ersten Reaktion auf die Einführung des Mindestlohns im Verlauf des Jahres 2015 die Anzahl der Übergänge in Beschäftigung ohne Leistungsbezug weiter zunimmt. Den Sprung aus der Bedürftigkeit von staatlichen Leistungen schaffen dabei vor allem sozialversicherungspflichtig beschäftigte Leistungsbezieher. Detailliert untersucht werden außerdem Beschäftigungsentwicklungen differenziert nach Geschlecht und Altersgruppen. Die erste Ausgabe des Arbeitsmarktspiegels erschien im Januar 2016 als IAB-Forschungsbericht 01/2016 (vom Berge et al., 2016). Dieser Forschungsbericht stellt eine aktualisierte und inhaltlich modifizierte Version dar.

    The second edition of the Arbeitsmarktspiegel describes important developments on the German labour market since the introduction of the minimum wage in Germany with latest data until January 2016. Overall employment continues to increase in 2015. The number of exclusively marginal employees, which decreased by a seasonally adjusted estimate of 93,000 persons at the turn of the year 2014/2015, shows a declining trend by the end of the third quarter of 2015. The bottom of this negative trend could be reached by winter. For the first time the second edition of the Arbeitsmarktspiegel shows that most of the transitions from exclusively marginal employment to exclusively employment covered by social security took place in the same establishment. With regard to employees receiving additional benefits according to Social Code Book II, the number of transitions into employment without benefit receipt further increases in the course of 2015 after their initial reaction following the introduction of the minimum wage. Especially those benefit recipients whose parallel employment is subject to social security are able to end eligibility for government benefits. Furthermore, the development of employment is examined in more detail by sex and age groups. The first edition of the Arbeitsmarktspiegel was released as IAB-Forschungsbericht 01/2016 (vom Berge et al., 2016) in January 2016. This research report represents an updated and substantially modified version.
    Language English
    Size 1 Online-Ressource (circa 78 Seiten), Illustrationen
    Publisher Institut für Arbeitsmarkt- und Berufsforschung der Bundesagentur für Arbeit
    Publishing place Nürnberg
    Document type Book ; Online
    Note Zusammenfassung in englischer Sprache
    Database ECONomics Information System

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