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  1. Article ; Online: A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST.

    Baleeva, Nadezhda S / Bogdanova, Yulia A / Goncharuk, Marina V / Sokolov, Anatolii I / Myasnyanko, Ivan N / Kublitski, Vadim S / Smirnov, Alexander Yu / Gilvanov, Aidar R / Goncharuk, Sergey A / Mineev, Konstantin S / Baranov, Mikhail S

    International journal of molecular sciences

    2024  Volume 25, Issue 5

    Abstract: NanoFAST is the smallest fluorogen-activating protein, consisting of only 98 amino acids, used as a genetically encoded fluorescent tag. Previously, only a single fluorogen with an orange color was revealed for this protein. In the present paper, using ... ...

    Abstract NanoFAST is the smallest fluorogen-activating protein, consisting of only 98 amino acids, used as a genetically encoded fluorescent tag. Previously, only a single fluorogen with an orange color was revealed for this protein. In the present paper, using rational mutagenesis and in vitro screening of fluorogens libraries, we expanded the color palette of this tag. We discovered that E46Q is one of the key substitutions enabling the range of possible fluorogens to be expanded. The introduction of this and several other substitutions has made it possible to use not only orange but also red and green fluorogens with the modified protein.
    MeSH term(s) Fluorescent Dyes/chemistry ; Proteins
    Chemical Substances Fluorescent Dyes ; Proteins
    Language English
    Publishing date 2024-03-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25053054
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Meta-CF

    Perfilov, Maxim M / Zaitseva, Elvira R / Baleeva, Nadezhda S / Kublitski, Vadim S / Smirnov, Alexander Yu / Bogdanova, Yulia A / Krasnova, Svetlana A / Myasnyanko, Ivan N / Mishin, Alexander S / Baranov, Mikhail S

    International journal of molecular sciences

    2023  Volume 24, Issue 12

    Abstract: In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a ... ...

    Abstract In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a pronounced solvent-dependent gradation of fluorescence intensity makes it possible to use these substances as fluorescent polarity sensors. In particular, we showed that one of the created compounds could be used for selective labeling of the endoplasmic reticulum of living cells.
    MeSH term(s) Green Fluorescent Proteins ; Solvents ; Coloring Agents ; Spectrometry, Fluorescence
    Chemical Substances Green Fluorescent Proteins (147336-22-9) ; Solvents ; Coloring Agents
    Language English
    Publishing date 2023-06-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24129923
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Total Synthesis of Racemic Thieno[3,2-

    Bolt, Yaroslav V / Dubinnyi, Maxim A / Litvinenko, Veronika V / Kotlobay, Alexey A / Belozerova, Olga A / Zagitova, Renata I / Shmygarev, Vladimir I / Yatskin, Oleg N / Guglya, Elena B / Kublitski, Vadim S / Baranov, Mikhail S / Yampolsky, Ilia V / Kaskova, Zinaida M / Tsarkova, Aleksandra S

    Organic letters

    2023  Volume 25, Issue 26, Page(s) 4892–4897

    Abstract: We report the first total synthesis of ... ...

    Abstract We report the first total synthesis of racemic
    Language English
    Publishing date 2023-06-27
    Publishing country United States
    Document type Journal Article
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.3c01696
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  4. Article ; Online: Proton-independent activation of acid-sensing ion channel 3 by an alkaloid, lindoldhamine, from Laurus nobilis.

    Osmakov, Dmitry I / Koshelev, Sergey G / Andreev, Yaroslav A / Dubinnyi, Maxim A / Kublitski, Vadim S / Efremov, Roman G / Sobolevsky, Alexander I / Kozlov, Sergey A

    British journal of pharmacology

    2018  Volume 175, Issue 6, Page(s) 924–937

    Abstract: Background and purpose: Acid-sensing ion channels (ASICs) play an important role in synaptic plasticity and learning, as well as in nociception and mechanosensation. ASICs are involved in pain and in neurological and psychiatric diseases, but their ... ...

    Abstract Background and purpose: Acid-sensing ion channels (ASICs) play an important role in synaptic plasticity and learning, as well as in nociception and mechanosensation. ASICs are involved in pain and in neurological and psychiatric diseases, but their therapeutic potential is limited by the lack of ligands activating them at physiological pH.
    Experimental approach: We extracted, purified and determined the structure of a bisbenzylisoquinoline alkaloid, lindoldhamine, (LIN) from laurel leaves. Its effect on ASIC3 channels were characterized, using two-electrode voltage-clamp electrophysiological recordings from Xenopus laevis oocytes.
    Key results: At pH 7.4 or higher, LIN activated a sustained, proton-independent, current through rat and human ASIC3 channels, but not rat ASIC1a or ASIC2a channels. LIN also potentiated proton-induced transient currents and promoted recovery from desensitization in human, but not rat, ASIC3 channels.
    Conclusions and implications: We describe a novel ASIC subtype-specific agonist LIN, which induced proton-independent activation of human and rat ASIC3 channels at physiological pH. LIN also acts as a positive allosteric modulator of human, but not rat, ASIC3 channels. This unique, species-selective, ligand of ASIC3, opens new avenues in studies of ASIC structure and function, as well as providing new approaches to drug design.
    MeSH term(s) Acid Sensing Ion Channels/drug effects ; Acid Sensing Ion Channels/metabolism ; Alkaloids/chemistry ; Alkaloids/isolation & purification ; Alkaloids/pharmacology ; Allosteric Regulation/drug effects ; Animals ; Female ; Humans ; Hydrogen-Ion Concentration ; Laurus/chemistry ; Oocytes ; Patch-Clamp Techniques ; Plant Leaves ; Protons ; Rats ; Species Specificity ; Xenopus laevis
    Chemical Substances ASIC3 protein, human ; ASIC3 protein, rat ; Acid Sensing Ion Channels ; Alkaloids ; Protons
    Language English
    Publishing date 2018-02-12
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80081-8
    ISSN 1476-5381 ; 0007-1188
    ISSN (online) 1476-5381
    ISSN 0007-1188
    DOI 10.1111/bph.14134
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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.146: Show issues Location:
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  5. Article: Sevanol and Its Analogues: Chemical Synthesis, Biological Effects and Molecular Docking.

    Belozerova, Olga A / Osmakov, Dmitry I / Vladimirov, Andrey / Koshelev, Sergey G / Chugunov, Anton O / Andreev, Yaroslav A / Palikov, Victor A / Palikova, Yulia A / Shaykhutdinova, Elvira R / Gvozd, Artem N / Dyachenko, Igor A / Efremov, Roman G / Kublitski, Vadim S / Kozlov, Sergey A

    Pharmaceuticals (Basel, Switzerland)

    2020  Volume 13, Issue 8

    Abstract: Among acid-sensing ion channels (ASICs), ASIC1a and ASIC3 subunits are the most widespread and prevalent in physiological and pathophysiological conditions. They participate in synaptic plasticity, learning and memory, as well as the perception of ... ...

    Abstract Among acid-sensing ion channels (ASICs), ASIC1a and ASIC3 subunits are the most widespread and prevalent in physiological and pathophysiological conditions. They participate in synaptic plasticity, learning and memory, as well as the perception of inflammatory and neurological pain, making these channels attractive pharmacological targets. Sevanol, a natural lignan isolated from
    Language English
    Publishing date 2020-07-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph13080163
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  6. Article ; Online: A novel type of luciferin from the Siberian luminous earthworm Fridericia heliota: structure elucidation by spectral studies and total synthesis.

    Petushkov, Valentin N / Dubinnyi, Maxim A / Tsarkova, Aleksandra S / Rodionova, Natalja S / Baranov, Mikhail S / Kublitski, Vadim S / Shimomura, Osamu / Yampolsky, Ilia V

    Angewandte Chemie (International ed. in English)

    2014  Volume 53, Issue 22, Page(s) 5566–5568

    Abstract: The structure elucidation and synthesis of the luciferin from the recently discovered luminous earthworm Fridericia heliota is reported. This luciferin is a key component of a novel ATP-dependent bioluminescence system. UV, fluorescence, NMR, and HRMS ... ...

    Abstract The structure elucidation and synthesis of the luciferin from the recently discovered luminous earthworm Fridericia heliota is reported. This luciferin is a key component of a novel ATP-dependent bioluminescence system. UV, fluorescence, NMR, and HRMS spectroscopy studies were performed on 0.005 mg of the isolated substance and revealed four isomeric structures that conform to spectral data. These isomers were chemically synthesized and one of them was found to produce light when reacted with a protein extract from F. heliota. The novel luciferin was found to have an unusual extensively modified peptidic nature, thus implying an unprecedented mechanism of action.
    MeSH term(s) Adenosine Triphosphate/metabolism ; Animals ; Isomerism ; Luminescent Agents/chemical synthesis ; Luminescent Agents/chemistry ; Luminescent Measurements ; Oligochaeta/metabolism ; Peptides/chemistry ; Siberia
    Chemical Substances Luminescent Agents ; Peptides ; Adenosine Triphosphate (8L70Q75FXE)
    Language English
    Publishing date 2014-05-26
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.201400529
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  7. Article ; Online: Bioluminescence chemistry of fireworm

    Kotlobay, Alexey A / Dubinnyi, Maxim A / Purtov, Konstantin V / Guglya, Elena B / Rodionova, Natalja S / Petushkov, Valentin N / Bolt, Yaroslav V / Kublitski, Vadim S / Kaskova, Zinaida M / Ziganshin, Rustam H / Nelyubina, Yulia V / Dorovatovskii, Pavel V / Eliseev, Igor E / Branchini, Bruce R / Bourenkov, Gleb / Ivanov, Igor A / Oba, Yuichi / Yampolsky, Ilia V / Tsarkova, Aleksandra S

    Proceedings of the National Academy of Sciences of the United States of America

    2019  Volume 116, Issue 38, Page(s) 18911–18916

    Abstract: ... Marine ... ...

    Abstract Marine polychaetes
    MeSH term(s) Animals ; Biosynthetic Pathways ; Color ; Indoles/chemistry ; Indoles/metabolism ; Luminescent Agents/chemistry ; Luminescent Agents/metabolism ; Luminescent Measurements ; Luminescent Proteins/metabolism ; Molecular Structure ; Oxidation-Reduction ; Polychaeta/chemistry ; Polychaeta/metabolism ; Pyrazines/chemistry ; Pyrazines/metabolism
    Chemical Substances Indoles ; Luminescent Agents ; Luminescent Proteins ; Pyrazines ; oxyluciferin (17297-78-8)
    Language English
    Publishing date 2019-08-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.1902095116
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
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