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Article ; Online: Enriching electroactive microorganisms from ferruginous lake waters - Mind the sulfate reducers!

Valero, Astolfo / Petrash, Daniel A / Kuchenbuch, Anne / Korth, Benjamin

Bioelectrochemistry (Amsterdam, Netherlands)

2024 Volume 157, Page(s) 108661

Abstract: Electroactive microorganisms are pivotal players in mineral transformation within redox interfaces characterized by pronounced oxygen and dissolved metal gradients. Yet, their systematic cultivation from such environments remains elusive. Here, we ... ...

Abstract	Electroactive microorganisms are pivotal players in mineral transformation within redox interfaces characterized by pronounced oxygen and dissolved metal gradients. Yet, their systematic cultivation from such environments remains elusive. Here, we conducted an anodic enrichment using anoxic ferruginous waters from a post-mining lake as inoculum. Weak electrogenicity (j = ∼5 µA cm
MeSH term(s)	Lakes/microbiology ; Sulfates ; Iron/chemistry ; Minerals ; Oxidation-Reduction ; Bacteria
Chemical Substances	Sulfates ; Iron (E1UOL152H7) ; Minerals
Language	English
Publishing date	2024-02-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2024.108661
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Article ; Online: Combining

Dzofou Ngoumelah, Daniel / Kuchenbuch, Anne / Harnisch, Falk / Kretzschmar, Jörg

Environmental science & technology

2023 Volume 57, Issue 6, Page(s) 2584–2594

Abstract: The combination of anaerobic digestion (AD) and microbial electrochemical technologies (METs) offers different opportunities to increase the efficiency and sustainability of AD processes. However, methanogenic archaea and/or particles may partially ... ...

Abstract	The combination of anaerobic digestion (AD) and microbial electrochemical technologies (METs) offers different opportunities to increase the efficiency and sustainability of AD processes. However, methanogenic archaea and/or particles may partially hinder combining MET and AD processes. Furthermore, it is unclear if the applied anode potential affects the activity and efficiency of electroactive microorganisms in AD-MET combinations as it is described for more controlled experimental conditions. In this study, we confirm that 6-week-old
MeSH term(s)	Bioelectric Energy Sources ; Geobacter ; Anaerobiosis ; Biofilms ; Electrodes
Language	English
Publishing date	2023-02-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.2c07574
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Article ; Online: Combining Geobacter spp. Dominated Biofilms and Anaerobic Digestion Effluents

Dzofou Ngoumelah, Daniel / Kuchenbuch, Anne / Harnisch, Falk / Kretzschmar, Jörg

The Effect of Effluent Composition and Electrode Potential on Biofilm Activity and Stability

2023

Keywords	Text ; ddc:333.7 ; anaerobic digestion ; applied anode potential ; Geobacter spp. dominated biofilm ; methanogens ; microbial electrochemical technologies
Language	English
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Redox Potential Heterogeneity in Fixed-Bed Electrodes Leads to Microbial Stratification and Inhomogeneous Performance.

Quejigo, Jose Rodrigo / Korth, Benjamin / Kuchenbuch, Anne / Harnisch, Falk

ChemSusChem

2021 Volume 14, Issue 4, Page(s) 1155–1165

Abstract: Bed electrodes provide high electrode area-to-volume ratios represent a promising configuration for transferring bioelectrochemical systems close to industrial applications. Nevertheless, the intrinsic electrical resistance leads to poor polarization ... ...

Abstract	Bed electrodes provide high electrode area-to-volume ratios represent a promising configuration for transferring bioelectrochemical systems close to industrial applications. Nevertheless, the intrinsic electrical resistance leads to poor polarization behavior. Therefore, the distribution of Geobacter spp. and their electrochemical performance within exemplary fixed-bed electrodes are investigated. A minimally invasive sampling system allows characterization of granules from different spatial locations of bed electrodes. Cyclic voltammetry of single granules (n=63) demonstrates that the major share of electroactivity (134.3 mA L
MeSH term(s)	Bioreactors/microbiology ; Electrochemistry/instrumentation ; Electrodes ; Geobacter/metabolism ; Oxidation-Reduction
Language	English
Publishing date	2021-01-19
Publishing country	Germany
Document type	Journal Article
ISSN	1864-564X
ISSN (online)	1864-564X
DOI	10.1002/cssc.202002611
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Article: Electrochemical Microwell Plate to Study Electroactive Microorganisms in Parallel and Real-Time.

Kuchenbuch, Anne / Frank, Ronny / Ramos, José Vazquez / Jahnke, Heinz-Georg / Harnisch, Falk

Frontiers in bioengineering and biotechnology

2022 Volume 9, Page(s) 821734

Abstract: Microbial resource mining of electroactive microorganism (EAM) is currently methodically hampered due to unavailable electrochemical screening tools. Here, we introduce an electrochemical microwell plate (ec-MP) composed of a 96 electrochemical deepwell ... ...

Abstract	Microbial resource mining of electroactive microorganism (EAM) is currently methodically hampered due to unavailable electrochemical screening tools. Here, we introduce an electrochemical microwell plate (ec-MP) composed of a 96 electrochemical deepwell plate and a recently developed 96-channel multipotentiostat. Using the ec-MP we investigated the electrochemical and metabolic properties of the EAM models
Language	English
Publishing date	2022-02-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2021.821734
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Article ; Online: Determining incremental coulombic efficiency and physiological parameters of early stage Geobacter spp. enrichment biofilms.

Korth, Benjamin / Kretzschmar, Jörg / Bartz, Manuel / Kuchenbuch, Anne / Harnisch, Falk

PloS one

2020 Volume 15, Issue 6, Page(s) e0234077

Abstract: Geobacter spp. enrichment biofilms were cultivated in batch using one-chamber and two-chamber bioelectrochemical reactors. Time-resolved substrate quantification was performed to derive physiological parameters as well as incremental coulombic efficiency ...

Abstract	Geobacter spp. enrichment biofilms were cultivated in batch using one-chamber and two-chamber bioelectrochemical reactors. Time-resolved substrate quantification was performed to derive physiological parameters as well as incremental coulombic efficiency (i.e., coulombic efficiency during one batch cycle, here every 6h) during early stage biofilm development. The results of one-chamber reactors revealed an intermediate acetate increase putatively due to the presence of acetogens. Total coulombic efficiencies of two-chamber reactors were considerable lower (19.6±8.3% and 49.3±13.2% for 1st and 2nd batch cycle, respectively) compared to usually reported values of mature Geobacter spp. enrichment biofilms presumably reflecting energetic requirements for biomass production (i.e., cells and extracellular polymeric substances) during early stages of biofilm development. The incremental coulombic efficiency exhibits considerable changes during batch cycles indicating shifts between phases of maximizing metabolic rates and maximizing biomass yield. Analysis based on Michaelis-Menten kinetics yielded maximum substrate uptake rates (vmax,Ac, vmax,I) and half-saturation concentration coefficients (KM,Ac,KM,I) based on acetate uptake or current production, respectively. The latter is usually reported in literature but neglects energy demands for biofilm growth and maintenance as well as acetate and electron storage. From 1st to 2nd batch cycle, vmax,Ac and KM,Ac, decreased from 0.0042-0.0051 mmol Ac- h-1 cm-2 to 0.0031-0.0037 mmol Ac- h-1 cm-2 and 1.02-2.61 mM Ac- to 0.28-0.42 mM Ac-, respectively. Furthermore, differences between KM,Ac/KM,I and vmax,Ac/vmax,I were observed providing insights into the physiology of Geobacter spp. enrichment biofilms. Notably, KM,I considerably scattered while vmax,Ac/vmax,I and KM,Ac remained rather stable indicating that acetate transport within biofilm only marginally affects reaction rates. The observed data variation mandates the requirement of a more detailed analysis with an improved experimental system, e.g., using flow conditions and a comparison with Geobacter spp. pure cultures.
MeSH term(s)	Acetates/analysis ; Acetates/metabolism ; Batch Cell Culture Techniques ; Biofilms/growth & development ; Biomass ; Chromatography, High Pressure Liquid ; Electron Transport ; Geobacter/metabolism ; Geobacter/physiology ; Kinetics
Chemical Substances	Acetates
Language	English
Publishing date	2020-06-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0234077
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Article ; Online: Label-Free Four-Dimensional Visualization of Anaerobically Growing Electroactive Biofilms.

Koch, Christin / Kuchenbuch, Anne / Marosvölgyi, Maria / Weisshart, Klaus / Harnisch, Falk

Cytometry. Part A : the journal of the International Society for Analytical Cytology

2020 Volume 97, Issue 7, Page(s) 737–741

Abstract: Light sheet fluorescence microscopy (LSFM) allows nondestructive, label-free and in vivo imaging of large specimen, even at nontransparent surfaces. We show that LSFM can be applied for label-free analyses of prokaryotes on the example of electroactive ... ...

Abstract	Light sheet fluorescence microscopy (LSFM) allows nondestructive, label-free and in vivo imaging of large specimen, even at nontransparent surfaces. We show that LSFM can be applied for label-free analyses of prokaryotes on the example of electroactive biofilms. Biofilm growth is linked to the production of current serving as measure of metabolic activity in vivo by monitoring with high spatial and temporal resolution. After 35 h of exponential growth, a homogeneous biofilm with a thickness of 9 μm was formed. This was followed by a stratification of the biofilm including the formation of 3D structures over the next 100 h. Light reflection was sufficient to visualize the biofilm structure and development over time and the terminal morphology was confirmed using fluorescence staining. This proof of concept on using LSFM for investigation of biofilms opens the door for its application in the entire field of microbial ecology. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC. on behalf of International Society for Advancement of Cytometry.
MeSH term(s)	Biofilms ; Microscopy, Fluorescence
Language	English
Publishing date	2020-06-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2099868-5
ISSN	1552-4930 ; 0196-4763 ; 1552-4922
ISSN (online)	1552-4930
ISSN	0196-4763 ; 1552-4922
DOI	10.1002/cyto.a.24169
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Zs.A 1688: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Long-term monitoring reveals stable and remarkably similar microbial communities in parallel full-scale biogas reactors digesting energy crops.

Lucas, Rico / Kuchenbuch, Anne / Fetzer, Ingo / Harms, Hauke / Kleinsteuber, Sabine

FEMS microbiology ecology

2015 Volume 91, Issue 3

Abstract: Biogas is an important renewable energy carrier. It is a product of stepwise anaerobic degradation of organic materials by highly diverse microbial communities forming complex interlinking metabolic networks. Knowledge about the microbial background of ... ...

Abstract	Biogas is an important renewable energy carrier. It is a product of stepwise anaerobic degradation of organic materials by highly diverse microbial communities forming complex interlinking metabolic networks. Knowledge about the microbial background of long-term stable process performance in full-scale reactors is crucial for rationally improving the efficiency and reliability of biogas plants. To generate such knowledge, in the present study three parallel mesophilic full-scale reactors fed exclusively with energy crops were sampled weekly over one year. Physicochemical process parameters were determined and the microbial communities were analysed by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting and 454-amplicon sequencing. For investigating the methanogenic community, a high-resolution T-RFLP approach based on the mcrA gene was developed by selecting restriction enzymes with improved taxonomic resolution compared to previous studies. Interestingly, no Methanosarcina-related generalists, but rather specialized hydrogenotrophic and acetoclastic methanogenic taxa were detected. In general, the microbial communities in the non-connected reactors were remarkably stable and highly similar indicating that identical environmental and process parameters resulted in identical microbial assemblages and dynamics. Practical implications such as flexible operation schemes comprising controlled variations of process parameters for an efficient microbial resource management under fluctuating process conditions are discussed.
MeSH term(s)	Actinobacteria/genetics ; Actinobacteria/metabolism ; Base Sequence ; Biofuels/microbiology ; Bioreactors/microbiology ; Chloroflexi/genetics ; Chloroflexi/metabolism ; Clostridium/genetics ; Clostridium/metabolism ; Crops, Agricultural/metabolism ; Crops, Agricultural/microbiology ; DNA Restriction Enzymes/genetics ; DNA, Archaeal/genetics ; Euryarchaeota/classification ; Euryarchaeota/genetics ; Euryarchaeota/metabolism ; Lactobacillus/genetics ; Lactobacillus/metabolism ; Microbiota/physiology ; Phylogeny ; Polymorphism, Restriction Fragment Length ; Reproducibility of Results ; Sequence Analysis, DNA ; Streptococcus/genetics ; Streptococcus/metabolism ; Zea mays/metabolism ; Zea mays/microbiology
Chemical Substances	Biofuels ; DNA, Archaeal ; DNA Restriction Enzymes (EC 3.1.21.-) ; endodeoxyribonuclease McrA (EC 3.1.21.-)
Language	English
Publishing date	2015-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	283722-5
ISSN	1574-6941 ; 0168-6496
ISSN (online)	1574-6941
ISSN	0168-6496
DOI	10.1093/femsec/fiv004
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Zs.A 2967: Show issues

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Article ; Online: Predicting and experimental evaluating bio-electrochemical synthesis - A case study with Clostridium kluyveri.

Koch, Christin / Kuchenbuch, Anne / Kracke, Frauke / Bernhardt, Paul V / Krömer, Jens / Harnisch, Falk

Bioelectrochemistry (Amsterdam, Netherlands)

2017 Volume 118, Page(s) 114–122

Abstract: Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central ... ...

Abstract	Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central for its further development is using appropriate electroactive microorganisms and their efficient extracellular electron transfer (EET) as well as wiring of the metabolism to EET. Among others, Clostridia are believed to represent electroactive microbes being highly promising for microbial electrosynthesis. We investigated the potential steps and challenges for the bio-electrochemical fermentation (electro-fermentation) of mid-chain organic acids using Clostridium kluyveri. Starting from a metabolic model the potential limitations of the metabolism as well as beneficial scenarios for electrochemical stimulation were identified and experimentally investigated. C. kluyveri was shown to not be able to exchange electrons with an electrode directly. Therefore, exogenous mediators (2-hydroxy-1,4-naphthoquinone, potassium ferrocyanide, neutral red, methyl viologen, methylene blue, and the macrocyclic cobalt hexaamine [Co(trans-diammac)]
MeSH term(s)	Clostridium kluyveri/drug effects ; Clostridium kluyveri/growth & development ; Clostridium kluyveri/metabolism ; Culture Media/chemistry ; Electrochemistry ; Electrodes ; Electron Transport ; Fatty Acids/biosynthesis ; Fatty Acids/chemistry ; Fermentation ; Hydrogen-Ion Concentration ; Models, Biological
Chemical Substances	Culture Media ; Fatty Acids
Language	English
Publishing date	2017-12
Publishing country	Netherlands
Document type	Evaluation Studies ; Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2017.07.009
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Article: Coupling electric energy and biogas production in anaerobic digesters – impacts on the microbiome

Koch, Christin / Harnisch, Falk / Kretzschmar, Jörg / Kuchenbuch, Anne / Liebetrau, Jan / Müller, Susann / Wedwitschka, Harald

RSC advances. 2015 Apr. 01, v. 5, no. 40

2015

Abstract: The combination of anaerobic digestion (AD) and microbial electrochemical technologies provides the opportunity to efficiently produce methane and electrical energy from complex biomass. Enhanced methane production and system stability have been reported ...

Abstract	The combination of anaerobic digestion (AD) and microbial electrochemical technologies provides the opportunity to efficiently produce methane and electrical energy from complex biomass. Enhanced methane production and system stability have been reported but the causes (electrolysis or microbial–electrochemical interactions) are less understood. Using the model substrate corn silage it is demonstrated that, for conditions allowing microbiome growth and adaptation, the methane yield of combined reactors remains constant (216 (±29) mL godm−1) while a second product, electrons (q = 14.4 (±0.8) kC, jmax = 1.34 mA cm−2 geometric current density), is also generated. The combined strategy allowed up to a 27% increase in total yield while the reactor community and its dynamics over time were not affected. A typical AD composition of Firmicutes, Bacteroidetes, Proteobacteria, and Synergistetes (bacteria) as well as Methanosarcina, Methanoculleus and Methanobacterium (archaea) was found in the bulk liquid. Specific enrichments of Geobacter (anode) and Methanobacterium (cathode) were of functional relevance.
Keywords	anaerobic digesters ; anaerobic digestion ; anodes ; bacteria ; Bacteroidetes ; biomass ; cathodes ; corn silage ; electric power ; electrochemistry ; electrolysis ; electrons ; Firmicutes ; gas production (biological) ; Geobacter ; geometry ; liquids ; methane ; methane production ; Methanobacterium ; Methanoculleus ; Methanosarcina ; microbiome ; Synergistetes
Language	English
Dates of publication	2015-0401
Size	p. 31329-31340.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c5ra03496e
Database	NAL-Catalogue (AGRICOLA)

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