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Article: Probing Effect of 6 MeV Electron Beam Irradiation on Haemoglobin Protein Using Spectroscopic Techniques.

Hinge, Sarika / Dhole, Sanjay / Banpurkar, Arun / Kulkarni, Gauri

Dose-response : a publication of International Hormesis Society

2024 Volume 22, Issue 2, Page(s) 15593258241240233

Abstract: In this work, we study the effect of 6 MeV electron beam irradiation on the physicochemical properties of lyophilized Human Haemoglobin A (HbA). Electron beams generated from Race Track Microtron accelerator with energy 6 MeV were used to irradiate HbA ... ...

Abstract	In this work, we study the effect of 6 MeV electron beam irradiation on the physicochemical properties of lyophilized Human Haemoglobin A (HbA). Electron beams generated from Race Track Microtron accelerator with energy 6 MeV were used to irradiate HbA at fluences of 5 × 10
Language	English
Publishing date	2024-04-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2440820-7
ISSN	1559-3258
ISSN	1559-3258
DOI	10.1177/15593258241240233
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality.

Kulkarni, Gauri / Apostolou, Athanasia / Ewart, Lorna / Lucchesi, Carolina / Kasendra, Magdalena

Journal of visualized experiments : JoVE

2022 , Issue 183

Abstract: The intestinal mucosa is a complex physical and biochemical barrier that fulfills a myriad of important functions. It enables the transport, absorption, and metabolism of nutrients and xenobiotics while facilitating a symbiotic relationship with ... ...

Abstract	The intestinal mucosa is a complex physical and biochemical barrier that fulfills a myriad of important functions. It enables the transport, absorption, and metabolism of nutrients and xenobiotics while facilitating a symbiotic relationship with microbiota and restricting the invasion of microorganisms. Functional interaction between various cell types and their physical and biochemical environment is vital to establish and maintain intestinal tissue homeostasis. Modeling these complex interactions and integrated intestinal physiology in vitro is a formidable goal with the potential to transform the way new therapeutic targets and drug candidates are discovered and developed. Organoids and Organ-on-a-Chip technologies have recently been combined to generate human-relevant intestine chips suitable for studying the functional aspects of intestinal physiology and pathophysiology in vitro. Organoids derived from the biopsies of the small (duodenum) and large intestine are seeded into the top compartment of an organ chip and then successfully expand as monolayers while preserving the distinct cellular, molecular, and functional features of each intestinal region. Human intestine tissue-specific microvascular endothelial cells are incorporated in the bottom compartment of the organ chip to recreate the epithelial-endothelial interface. This novel platform facilitates luminal exposure to nutrients, drugs, and microorganisms, enabling studies of intestinal transport, permeability, and host-microbe interactions. Here, a detailed protocol is provided for the establishment of intestine chips representing the human duodenum (duodenum chip) and colon (colon chip), and their subsequent culture under continuous flow and peristalsis-like deformations. We demonstrate methods for assessing drug metabolism and CYP3A4 induction in duodenum chip using prototypical inducers and substrates. Lastly, we provide a step-by-step procedure for the in vitro modeling of interferon gamma (IFNγ)-mediated barrier disruption (leaky gut syndrome) in a colon chip, including methods for evaluating the alteration of paracellular permeability, changes in cytokine secretion, and transcriptomic profiling of the cells within the chip.
MeSH term(s)	Endothelial Cells ; Humans ; Intestinal Mucosa/metabolism ; Lab-On-A-Chip Devices ; Organoids ; Technology
Language	English
Publishing date	2022-05-05
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/63724
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Article ; Online: Investigating alterations in the cellular envelope of Staphylococcus aureus in simulated microgravity using a random positioning machine.

Singh, Sandhya / Vidyasagar, Pandit B / Kulkarni, Gauri R

Life sciences in space research

2021 Volume 30, Page(s) 1–8

Abstract: Continuous rotation of liquid bacterial culture in random positioning machine (RPM) causes formation of a colloidal bacterial culture in the culture tube, due to lack of sedimentation and convection. Interestingly, similar colloidal bacterial cultures ... ...

Abstract	Continuous rotation of liquid bacterial culture in random positioning machine (RPM) causes formation of a colloidal bacterial culture in the culture tube, due to lack of sedimentation and convection. Interestingly, similar colloidal bacterial cultures can also be seen in suspended bacterial cultures in a spaceflight environment. Thus, as a consequence of no sedimentation, an alteration in the microenvironment of each bacterial cell in simulated microgravity is introduced, compared to the bacterial culture grown in normal gravity wherein they sediment slowly at the bottom of the culture tube. Apparently, a bacterial cell can sense changes in its environment through various receptors and sensors present at its surface, thus it can be speculated that this change in its microenvironment might induce changes in its cell wall and cell surface properties. In our study, changes in growth kinetics, cell wall constitution using FTIR (Fourier Transform Infrared Spectroscopy), cell surface hydrophobicity, autoaggregation ability and antibiotic susceptibility of Staphylococcus aureus NCIM 2079 strain, in simulated microgravity (using RPM) was studied in detail. Noteworthy alterations in its growth kinetics, cell wall constitution, cell surface hydrophobicity, autoaggregation ability and antibiotic susceptibility especially to Erythromycin and Clindamycin were observed. Our data suggests that microgravity may cause alterations in the cellular envelope of planktonic S.aureus cultures.
MeSH term(s)	Cell Wall ; Space Flight ; Staphylococcus aureus ; Weightlessness ; Weightlessness Simulation
Language	English
Publishing date	2021-04-06
Publishing country	Netherlands
Document type	Journal Article
ISSN	2214-5532
ISSN (online)	2214-5532
DOI	10.1016/j.lssr.2021.04.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Flow cytometry analysis of SH-SY-5Y cells treated with MN304 nanoparticles

Beedkar, Anuradha Narendra / Kulkarni, Gauri Rajendra

BIOINFOLET. 2021 Dec., v. 18, no. 4

2021

Abstract: Effect of Mn₃O₄nanoparticles on SH-SY-5Y neuroblastoma cell line was studied. SH-SY-5Y cell line was treated with different concentrations of Mn₃O₄ nanoparticles for 24 hours. Toxicity of Mn₃O₄ nanoparticles was quantified by MTT assay, Annexin Pi ... ...

Abstract	Effect of Mn₃O₄nanoparticles on SH-SY-5Y neuroblastoma cell line was studied. SH-SY-5Y cell line was treated with different concentrations of Mn₃O₄ nanoparticles for 24 hours. Toxicity of Mn₃O₄ nanoparticles was quantified by MTT assay, Annexin Pi apoptosis assay, Cell Cycle Analysis, ROS assay and microscopic observations. It was observed that Mn₃O₄ nanoparticles are not toxic to SH-SY-5Y cell line at lower concentration. However decreased cell viability and cell death by necrosis at higher concentration of Mn₃O₄ nanoparticles proposes appropriate quantity of nanoparticles have potential in biomedical application.
Keywords	apoptosis ; cell cycle ; cell lines ; cell viability ; flow cytometry ; necrosis ; toxicity ; toxicity testing
Language	English
Dates of publication	2021-12
Size	p. 490-496.
Publishing place	Dr. Anil M. Mungikar
Document type	Article
ISSN	0976-4755
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Comparative studies on measurement of membrane potential of bacterial cells treated with ZnO nanoparticles by Spectrofluorometry, fluorescence microscopy and flowcytometry.

Khater, Maya / Khater, Sagar S / Gholap, Haribhau / Patil, Rajendra / Kulkarni, Gauri

Journal of microbiological methods

2020 Volume 173, Page(s) 105920

Abstract: Many methods are developed to assess antimicrobial action of ZnO nanoparticles (NPs). A large number of methods associated with the use of fluorescent probes are developed, including Spectrofluorometry, fluorescence microscopy, and cytometry. In this ... ...

Abstract	Many methods are developed to assess antimicrobial action of ZnO nanoparticles (NPs). A large number of methods associated with the use of fluorescent probes are developed, including Spectrofluorometry, fluorescence microscopy, and cytometry. In this study, flowcytometry, Spectrofluorometry and fluorescent microscopy was used to measure membrane potential variation of E. coli and S. aureus cells treated with two different sizes of zinc oxide (ZnO) NPs and were compared with conventional methods. In order to estimate change in membrane potential, E. coli and S. aureus cells were treated with iopnophore agent carbonyl cyanide m-chlorophenylhydrazone (CCCP) and membrane potential was evaluated using fluorescent probe 3,3'-Diethyloxacarbocyanine, iodide (DIOC
MeSH term(s)	Anti-Bacterial Agents ; Bacteria/drug effects ; Bacteria/metabolism ; Escherichia coli ; Flow Cytometry/methods ; Membrane Potentials/physiology ; Microbial Sensitivity Tests ; Microscopy, Fluorescence/methods ; Nanoparticles/chemistry ; Reactive Oxygen Species/metabolism ; Spectrometry, Fluorescence/methods ; Staphylococcus aureus ; Zinc Oxide/pharmacology
Chemical Substances	Anti-Bacterial Agents ; Reactive Oxygen Species ; Zinc Oxide (SOI2LOH54Z)
Language	English
Publishing date	2020-04-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604916-3
ISSN	1872-8359 ; 0167-7012
ISSN (online)	1872-8359
ISSN	0167-7012
DOI	10.1016/j.mimet.2020.105920
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Article ; Online: Synthesis of α3β4 Nicotinic Acetylcholine Receptor Modulators Derived from Aristoquinoline That Reduce Reinstatement of Cocaine-Seeking Behavior.

Rusali, Lisa E / Lopez-Hernandez, Ana M / Kremiller, Kyle M / Kulkarni, Gauri C / Gour, Abhishek / Straub, Carolyn J / Argade, Malaika D / Peters, Christian J / Sharma, Abhisheak / Toll, Lawrence / Cippitelli, Andrea / Riley, Andrew P

Journal of medicinal chemistry

2023 Volume 67, Issue 1, Page(s) 529–542

Abstract: Growing evidence suggests that inhibition of the α3β4 nicotinic acetylcholine receptor (nAChR) represents a promising therapeutic strategy to treat cocaine use disorder. Recently, aristoquinoline ( ...

Abstract	Growing evidence suggests that inhibition of the α3β4 nicotinic acetylcholine receptor (nAChR) represents a promising therapeutic strategy to treat cocaine use disorder. Recently, aristoquinoline (
MeSH term(s)	Animals ; Receptors, Nicotinic ; Cocaine ; Alkaloids/pharmacology ; Alkaloids/therapeutic use ; Drug-Seeking Behavior ; Nicotinic Antagonists/pharmacology ; Nicotinic Antagonists/therapeutic use ; Quinolines
Chemical Substances	aristoquinoline ; Receptors, Nicotinic ; Cocaine (I5Y540LHVR) ; Alkaloids ; Nicotinic Antagonists ; Quinolines
Language	English
Publishing date	2023-12-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	218133-2
ISSN	1520-4804 ; 0022-2623
ISSN (online)	1520-4804
ISSN	0022-2623
DOI	10.1021/acs.jmedchem.3c01758
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Zs.B 151: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Examining the dynamics of consumer interest and live performance event ticket sales in the presence of a critical industry-wide event

Tseng, Peggy H / Kulkarni, Gauri

Review of marketing science Bd. 11.2013, Article 1, S. 33-53

2013

Author's details	Peggy H. Tseng and Gauri Kulkarni
Keywords	online search ; consumer interest ; ticket sales ; fixed effects
Language	English
Size	Online-Ressource, graph. Darst.
Publisher	De Gruyter
Publishing place	Berlin
Document type	Article ; Online
Note	IMD-Felder maschinell generiert
ZDB-ID	2130373-3
ISSN	1546-5616
DOI	10.1515/roms-2012-0002
Database	ECONomics Information System

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Article ; Online: Hitting the sweet spot.

Kulkarni, Gauri / Wadsworth, William G

Nature methods

2012 Volume 9, Issue 5, Page(s) 451, 453

MeSH term(s)	Animals ; Animals, Genetically Modified ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/immunology ; Epitopes/analysis ; Epitopes/genetics ; Epitopes/immunology ; Green Fluorescent Proteins/analysis ; Green Fluorescent Proteins/chemistry ; Green Fluorescent Proteins/genetics ; Heparitin Sulfate/chemistry ; Heparitin Sulfate/genetics ; Heparitin Sulfate/immunology ; Single-Chain Antibodies/analysis ; Single-Chain Antibodies/genetics ; Single-Chain Antibodies/immunology
Chemical Substances	Epitopes ; Single-Chain Antibodies ; Green Fluorescent Proteins (147336-22-9) ; Heparitin Sulfate (9050-30-0)
Language	English
Publishing date	2012-04-27
Publishing country	United States
Document type	News
ZDB-ID	2169522-2
ISSN	1548-7105 ; 1548-7091
ISSN (online)	1548-7105
ISSN	1548-7091
DOI	10.1038/nmeth.1987
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Article ; Online: Ellagic Acid Enhances Apoptotic Sensitivity of Breast Cancer Cells to γ-Radiation.

Ahire, Vidhula / Kumar, Amit / Mishra, Kaushala Prasad / Kulkarni, Gauri

Nutrition and cancer

2017 Volume 69, Issue 6, Page(s) 904–910

Abstract: Herbal polyphenols have gained increased significance because of the promises they hold in the prevention and treatment of cancer. There exists an enormous opportunity for the screening and valuation of natural dietary compounds in the development of an ... ...

Abstract	Herbal polyphenols have gained increased significance because of the promises they hold in the prevention and treatment of cancer. There exists an enormous opportunity for the screening and valuation of natural dietary compounds in the development of an effective chemopreventive drug and radiosensitizer that may be of practical use for patients undergoing cancer therapy. This study describes the effect of the flavonoid ellagic acid (EA) on gamma-irradiated human breast cancer MCF-7 cells in vitro when administered alone or in combination with radiation. It was interesting to find the radioprotective effect of EA on NIH3T3, which is a normal cell line. Irradiation of breast tumor cells in the presence of EA (10 μM) to doses of 2 and 4-Gy gamma radiation produced a marked synergistic tumor cytotoxicity while it was found to aid recovery from the radiation damage to NIH3T3 cells. When cells were given a combined treatment of EA and radiation, the cell death increased to 21.7% and 20.7% in the 2 and 4-Gy-treated cells respectively, significantly (P < 0.05) reducing the capacity of MCF-7 cells to form colonies. Even at 24 h, 38 foci/cell were observed in samples that were given the combined treatment, suggesting the cells' inability in repairing the damage. Also, increased apoptosis in EA+ 2Gy (50%) and EA+ 4 Gy (62%)-treated cells was observed in the the sub-G1 phase of the cell cycle. A 6.2-fold decrease in the mitochondrial membrane potential was observed in the combined treatment of EA and IR that facilitated the upregulation of pro-apopttotic Bax and downregulation of Bcl-2, pushing the MCF-7 cells to undergo an apoptotic cell death. It is suggested that EA may be a potential drug adjuvant for improving cancer radiotherapy by increasing tumor toxicity and reducing the normal cell damage caused by irradiation.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Apoptosis/radiation effects ; Breast Neoplasms/pathology ; Cell Cycle/drug effects ; Cell Cycle/radiation effects ; Cell Survival/drug effects ; Cell Survival/radiation effects ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Ellagic Acid/pharmacology ; Female ; Gamma Rays/therapeutic use ; Humans ; MCF-7 Cells ; Membrane Potential, Mitochondrial/drug effects ; Membrane Potential, Mitochondrial/radiation effects ; Mice ; NIH 3T3 Cells ; Sensitivity and Specificity
Chemical Substances	Ellagic Acid (19YRN3ZS9P)
Language	English
Publishing date	2017-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	424433-3
ISSN	1532-7914 ; 0163-5581
ISSN (online)	1532-7914
ISSN	0163-5581
DOI	10.1080/01635581.2017.1339811
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Article: Enhancement of magnetotactic bacterial yield in a modified MSGM medium without alteration of magnetosomes properties.

Kundu, Srikanya / Kulkarni, Gauri R

Indian journal of experimental biology

2010 Volume 48, Issue 5, Page(s) 518–523

Abstract: Magnetotactic bacteria (MTB), Magnetospirillum magnetotacticum (MS-1) were successfully grown in modified magnetic spirillum growth medium (MSGM) at normal laboratory environment. About five-time increase in the bacterial yield was achieved in the ... ...

Abstract	Magnetotactic bacteria (MTB), Magnetospirillum magnetotacticum (MS-1) were successfully grown in modified magnetic spirillum growth medium (MSGM) at normal laboratory environment. About five-time increase in the bacterial yield was achieved in the modified MSGM medium without compromising their magnetosomes properties. Transmission electron and scanning electron microscopy (TEM & SEM) were used for morphological study of MTB. Energy dispersive analysis of X-rays (EDAX) and vibrating sample magnetometer (VSM) techniques, respectively, were used to elucidate the phase and magnetization in the bacterially synthesized magnetosomes. These studies were important to cross-check the morphology of magnetosomes, as the formation of magnetosomes was highly sensitive to environmental conditions.
MeSH term(s)	Bacteriological Techniques ; Culture Media/pharmacology ; Electron Probe Microanalysis ; Ferrosoferric Oxide/analysis ; Magnetics ; Magnetosomes/physiology ; Magnetosomes/ultrastructure ; Magnetospirillum/drug effects ; Magnetospirillum/physiology ; Magnetospirillum/ultrastructure ; Microscopy, Electron, Scanning
Chemical Substances	Culture Media ; Ferrosoferric Oxide (XM0M87F357)
Language	English
Publishing date	2010-05
Publishing country	India
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	416061-7
ISSN	0975-1009 ; 0019-5189
ISSN (online)	0975-1009
ISSN	0019-5189
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