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Singh, Dharmendra Prasad / Kumar Verma, Rajesh / Sarswat, Swati / Saraswat, Satender

2022 Volume 7, Issue 2, Page(s) 8–13

Abstract: Background and purpose: The predominant cause of candidiasis was Candida albicans which has recently changed to non-: Materials and methods: Routine samples for bacterial culture and sensitivity that showed colony characteristics, like : Results: ... ...

Abstract	Background and purpose: The predominant cause of candidiasis was Candida albicans which has recently changed to non- Materials and methods: Routine samples for bacterial culture and sensitivity that showed colony characteristics, like Results: Mean age of the patients was 38.46 years with a male-female ratio of 1.36:1. In total, 137 Conclusion: Results of this study suggest varied prevalence and virulence based on geographical locations, even within a subcontinent. It clearly indicates the emergence of the NCAC spp. and their predominance in different body fluids. Identification of
Language	English
Publishing date	2022-01-09
Publishing country	Iran
Document type	Journal Article
ZDB-ID	3015705-5
ISSN	2423-3420 ; 2423-3439
ISSN (online)	2423-3420
ISSN	2423-3439
DOI	10.18502/cmm.7.2.7032
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Article ; Online: Effects of Secondary Alveolar Bone Grafting on Maxillary Growth in Cleft Lip or Palate Patients: A Systematic Review and Meta-Analysis.

Kannan, Phavithrasri / Verma, Sanjeev / Kumar, Vinay / Kumar Verma, Raj / Pal Singh, Satinder

The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association

2023 , Page(s) 10556656231188600

Abstract: To assess the effect of secondary alveolar bone grafting (SABG) on maxillary growth in patients with unilateral cleft lip or palate (UCL/P).: Systematic review and Meta-analysis.: Pubmed, Cochrane Library, Embase, Scopus, Web of Science and manual ... ...

Abstract	To assess the effect of secondary alveolar bone grafting (SABG) on maxillary growth in patients with unilateral cleft lip or palate (UCL/P). Systematic review and Meta-analysis. Pubmed, Cochrane Library, Embase, Scopus, Web of Science and manual search was performed to assess the maxillary growth following SABG in UCL/P patients. Non-syndromic patients with UCL/P. Comparison of maxillary growth between patients with UCL/P who underwent SABG and UCL/P patients who had not undergone SABG or non-cleft control. 39 of the identified 233 articles were assessed for inclusion and exclusion criteria after duplicate removal and title and abstract reading. 7 articles (1 prospective, and 6 retrospective studies) were included in the qualitative analysis and 2 articles were subjected to quantitative analysis. Four studies had a low risk of bias and three studies had a moderate risk of bias. Meta-analysis revealed a significant reduction of SNA and no significant difference in ANB in the SABG group compared to the non-cleft control group(I The studies showed a low to moderate risk of bias. SABG causes inhibition of maxillary growth in patients with cleft lip or palate when compared to patients with non-cleft control. Due to high heterogeneity, comparison to cleft control showed insufficient evidence.
Language	English
Publishing date	2023-07-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	1069409-2
ISSN	1545-1569 ; 0009-8701 ; 1055-6656
ISSN (online)	1545-1569
ISSN	0009-8701 ; 1055-6656
DOI	10.1177/10556656231188600
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Article: Dexmedetomidine-Ketamine or Dexmedetomidine-Midazolam Nebulised Drug Combination as a Premedicant in Children: A Randomised Clinical Trial.

Dhiman, Tanvi / Verma, Versha / Kumar Verma, Ravinder / Rana, Shelly / Singh, Jai / Badhan, Isha

Turkish journal of anaesthesiology and reanimation

2022 Volume 50, Issue 5, Page(s) 380–387

Abstract: Objective: This study was designed to evaluate the clinical efficacy of 2 low-dose nebulised drug combinations of dexmedetomidine-ketamine and dexmedetomidine-midazolam as a premedication in children scheduled for surgery under general anaesthesia.: ... ...

Abstract	Objective: This study was designed to evaluate the clinical efficacy of 2 low-dose nebulised drug combinations of dexmedetomidine-ketamine and dexmedetomidine-midazolam as a premedication in children scheduled for surgery under general anaesthesia. Methods: Sixty children classified as American Society of Anesthesiologists physical status I, aged between 3 and 10, listed to undergo elective surgeries under general anaesthesia were enrolled in this prospective, randomised, and double-blind trial. Patients were randomly allocated to receive nebulised premedication approximately 30 minutes before the induction of anaesthesia. Group DK (n=30) received combined nebulised dexmedetomidine and ketamine (1 μg kg-1+1 mg kg-1 ) and the dexmedetomidine-midazolam (DM) group (n=30) received combined nebulised dexmedetomidine and midazolam (1 μg kg-1+0.1 mg kg-1 ). All children were anaesthetised with a protocolised anaesthesia technique. The primary end point was the level of sedation when the child was first seen in the operating room 30 minutes after nebulisation. The secondary end points were parental separation and ease of induction, ease of acceptance of IV cannula, mask acceptance, postoperative analgesia, and wake-up behaviour. Results: Studied groups were comparable in demographic data (age, weight, and sex) and duration of anaesthesia. Level of sedation at 30 minutes was significantly greater in the DM group than in the DK group (P =.013) while the two were comparable in parental separation and ease of induction (P =.808). Group DK exhibited superior ease of acceptance of IV cannula (P =.001), mask acceptance score (P =.001), and postoperative analgesia (P =.021). Hemodynamic parameters and oxygen saturation remained comparable at all time intervals as also the wake-up behaviour. Conclusions: The nebulised combination of low-dose ketamine and dexmedetomidine was a superior combination producing acceptable sedation with enhanced ease of IV acceptance, mask acceptance, and postoperative analgesia in children.
Language	English
Publishing date	2022-10-27
Publishing country	Turkey
Document type	Journal Article
ISSN	2667-677X
ISSN	2667-677X
DOI	10.5152/TJAR.2022.21298
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Article ; Online: Xanthomonas oryzae pv. oryzae chemotaxis components and chemoreceptor Mcp2 are involved in the sensing of constituents of xylem sap and contribute to the regulation of virulence-associated functions and entry into rice.

Kumar Verma, Raj / Samal, Biswajit / Chatterjee, Subhadeep

Molecular plant pathology

2018 Volume 19, Issue 11, Page(s) 2397–2415

Abstract: The Xanthomonas group of phytopathogens causes several economically important diseases in crops. In the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo), it has been proposed that chemotaxis may play a role in the entry and colonization of ...

Abstract	The Xanthomonas group of phytopathogens causes several economically important diseases in crops. In the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo), it has been proposed that chemotaxis may play a role in the entry and colonization of the pathogen inside the host. However, components of the chemotaxis system, including the chemoreceptors involved, and their role in entry and virulence, are not well defined. In this study, we show that Xoo displays a positive chemotaxis response to components of rice xylem sap-glutamine, xylose and methionine. In order to understand the role of chemotaxis components involved in the promotion of chemotaxis, entry and virulence, we performed detailed deletion mutant analysis. Analysis of mutants defective in chemotaxis components, flagellar biogenesis, expression analysis and assays of virulence-associated functions indicated that chemotaxis-mediated signalling in Xoo is involved in the regulation of several virulence-associated functions, such as motility, attachment and iron homeostasis. The ∆cheY1 mutant of Xoo exhibited a reduced expression of genes involved in motility, adhesins, and iron uptake and metabolism. We show that the expression of Xoo chemotaxis and motility components is induced under in planta conditions and is required for entry, colonization and virulence. Furthermore, deletion analysis of a putative chemoreceptor mcp2 gene revealed that chemoreceptor Mcp2 is involved in the sensing of xylem sap and constituents of xylem exudate, including methionine, serine and histidine, and plays an important role in epiphytic entry and virulence. This is the first report of the role of chemotaxis in the virulence of this important group of phytopathogens.
MeSH term(s)	Bacterial Adhesion ; Bacterial Proteins/metabolism ; Biofilms/growth & development ; Chemotaxis/genetics ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Iron/metabolism ; Models, Biological ; Multigene Family ; Mutation/genetics ; Operon/genetics ; Oryza/microbiology ; Plant Exudates/metabolism ; Plant Leaves/microbiology ; Siderophores/metabolism ; Transcription, Genetic ; Virulence ; Xanthomonas/cytology ; Xanthomonas/genetics ; Xanthomonas/pathogenicity ; Xylem/metabolism
Chemical Substances	Bacterial Proteins ; Plant Exudates ; Siderophores ; Iron (E1UOL152H7)
Language	English
Publishing date	2018-08-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020755-4
ISSN	1364-3703 ; 1364-3703
ISSN (online)	1364-3703
ISSN	1364-3703
DOI	10.1111/mpp.12718
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Article: Xanthomonas oryzae pv. oryzae chemotaxis components and chemoreceptor Mcp2 are involved in the sensing of constituents of xylem sap and contribute to the regulation of virulence‐associated functions and entry into rice

Kumar Verma, Raj / Samal, Biswajit / Chatterjee, Subhadeep

Molecular plant pathology. 2018 Nov., v. 19, no. 11

2018

Abstract	The Xanthomonas group of phytopathogens causes several economically important diseases in crops. In the bacterial pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo), it has been proposed that chemotaxis may play a role in the entry and colonization of the pathogen inside the host. However, components of the chemotaxis system, including the chemoreceptors involved, and their role in entry and virulence, are not well defined. In this study, we show that Xoo displays a positive chemotaxis response to components of rice xylem sap—glutamine, xylose and methionine. In order to understand the role of chemotaxis components involved in the promotion of chemotaxis, entry and virulence, we performed detailed deletion mutant analysis. Analysis of mutants defective in chemotaxis components, flagellar biogenesis, expression analysis and assays of virulence‐associated functions indicated that chemotaxis‐mediated signalling in Xoo is involved in the regulation of several virulence‐associated functions, such as motility, attachment and iron homeostasis. The ∆cheY1 mutant of Xoo exhibited a reduced expression of genes involved in motility, adhesins, and iron uptake and metabolism. We show that the expression of Xoo chemotaxis and motility components is induced under in planta conditions and is required for entry, colonization and virulence. Furthermore, deletion analysis of a putative chemoreceptor mcp2 gene revealed that chemoreceptor Mcp2 is involved in the sensing of xylem sap and constituents of xylem exudate, including methionine, serine and histidine, and plays an important role in epiphytic entry and virulence. This is the first report of the role of chemotaxis in the virulence of this important group of phytopathogens.
Keywords	Xanthomonas oryzae pv. oryzae ; adhesins ; biogenesis ; chemoreceptors ; chemotaxis ; crops ; epiphytes ; gene expression ; genes ; histidine ; homeostasis ; iron ; metabolism ; methionine ; mutants ; plant pathogens ; rice ; sap ; serine ; virulence ; xylem ; xylose
Language	English
Dates of publication	2018-11
Size	p. 2397-2415.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2020755-4
ISSN	1364-3703 ; 1464-6722
ISSN (online)	1364-3703
ISSN	1464-6722
DOI	10.1111/mpp.12718
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Article ; Online: An efficient process for the extraction of lutein and chemical characterization of other organic volatiles from marigold (Tagetes erecta L.) flower.

Kumar Kashyap, Praveen / Singh, Swati / Kumar Singh, Munmun / Gupta, Atul / Tandon, Sudeep / Shanker, Karuna / Kumar Verma, Rajesh / Swaroop Verma, Ram

Food chemistry

2022 Volume 396, Page(s) 133647

Abstract: Marigold (Tagetes erecta L.) petals are the primary industrial source of lutein, which is used as a colouring agent and nutrient supplement to foods. This research extracted marigold petals using different solvents, covering conventional and non-toxic ... ...

Abstract	Marigold (Tagetes erecta L.) petals are the primary industrial source of lutein, which is used as a colouring agent and nutrient supplement to foods. This research extracted marigold petals using different solvents, covering conventional and non-toxic green solvents. The oleoresin, free lutein, and recrystallized lutein yields varied from 8.47-16.67%, 2.56-9.62%, and 1.11-1.61%, respectively. The purity of lutein was achieved up to 92.57% and 97.64% in conventional and newly established green methods, respectively. The present study described an efficient green process to isolate lutein with significantly improved yield (2.56%) and purity (97.33%) over the conventional methods. Based on the results, 2-methyltetrahydrofurancould be a practical green alternative to the traditional toxic solvents for the processing of lutein. Further, the chemical analysis of the essential oil of the residual receptacles obtained after removing petals revealed the presence of important organic volatiles, including piperitone (54.7%) and piperitenone oxide (6.5%), indicating its usefulness for value-addition.
MeSH term(s)	Calendula ; Flowers/chemistry ; Lutein/chemistry ; Solvents ; Tagetes/chemistry
Chemical Substances	Solvents ; Lutein (X72A60C9MT)
Language	English
Publishing date	2022-07-08
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.133647
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Article ; Online: Clarification, malocclusion classification, and comparison of two types of miniplates-request for further information.

Kumar, Vinay / Pal Singh, Satinder / Verma, Sanjeev / Kumar Verma, Raj

Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie

2019 Volume 81, Issue 4, Page(s) 301–302

Title translation	Erläuterung, Klassifikation der Malokklusion und Vergleich von 2 Miniplattentypen – Ersuchen um weitere Informationen.
MeSH term(s)	Humans ; Malocclusion ; Orthodontic Anchorage Procedures
Language	English
Publishing date	2019-07-03
Publishing country	Germany
Document type	Letter ; Comment
ZDB-ID	1309470-1
ISSN	1615-6714 ; 0015-816X ; 1434-5293
ISSN (online)	1615-6714
ISSN	0015-816X ; 1434-5293
DOI	10.1007/s00056-020-00233-7
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Article ; Online: Synthesis and Photophysical Properties of Fluorescence Molecular Probe for Turn-ON-Type Detection of Cytosine Bulge DNA.

Kumar Verma, Rajiv / Takei, Fumie / Nakatani, Kazuhiko

Organic letters

2016 Volume 18, Issue 13, Page(s) 3170–3173

Abstract: A fluorescent molecule DANP that binds to cytosine bulge DNA and emits characteristic fluorescence was conjugated to pyrene to give a new fluorescence probe PyDANP. Temperature- and solvent-dependent absorption changes showed that DANP and pyrene ... ...

Abstract	A fluorescent molecule DANP that binds to cytosine bulge DNA and emits characteristic fluorescence was conjugated to pyrene to give a new fluorescence probe PyDANP. Temperature- and solvent-dependent absorption changes showed that DANP and pyrene chromophores stacked at room temperature in an aqueous buffer solution and quenched fluorescence. Upon binding of DANP moiety in PyDANP to cytosine bulge DNA, the fluorescence from DANP bound to C-bulge increased by ∼12-fold, showing that PyDANP is a turn-ON probe for the detection of C-bulge DNA.
MeSH term(s)	Base Sequence ; Cytosine/chemistry ; DNA/chemistry ; Fluorescent Dyes/chemical synthesis ; Inverted Repeat Sequences ; Molecular Probes/chemical synthesis ; Naphthyridines/chemical synthesis ; Nucleic Acid Conformation ; Spectrometry, Fluorescence ; Staining and Labeling
Chemical Substances	Fluorescent Dyes ; Molecular Probes ; Naphthyridines ; Cytosine (8J337D1HZY) ; DNA (9007-49-2)
Language	English
Publishing date	2016--01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1523-7052
ISSN (online)	1523-7052
DOI	10.1021/acs.orglett.6b01378
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Article: Synthesis and Photophysical Properties of Fluorescence Molecular Probe for Turn-ON-Type Detection of Cytosine Bulge DNA

Kumar Verma, Rajiv / Takei Fumie / Nakatani Kazuhiko

Organic letters. 2016 July 01, v. 18, no. 13

2016

Abstract	A fluorescent molecule DANP that binds to cytosine bulge DNA and emits characteristic fluorescence was conjugated to pyrene to give a new fluorescence probe PyDANP. Temperature- and solvent-dependent absorption changes showed that DANP and pyrene chromophores stacked at room temperature in an aqueous buffer solution and quenched fluorescence. Upon binding of DANP moiety in PyDANP to cytosine bulge DNA, the fluorescence from DANP bound to C-bulge increased by â¼12-fold, showing that PyDANP is a turn-ON probe for the detection of C-bulge DNA.
Keywords	DNA ; absorption ; ambient temperature ; chemical reactions ; cytosine ; fluorescence ; fluorescent dyes ; moieties
Language	English
Dates of publication	2016-0701
Size	p. 3170-3173.
Publishing place	American Chemical Society
Document type	Article
ISSN	1523-7052
DOI	10.1021%2Facs.orglett.6b01378
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Article ; Online: Mycobactericidal activity of some micro-encapsulated synthetic Host Defense Peptides (HDP) by expediting the permeation of antibiotic: A new paradigm of drug delivery for tuberculosis.

Sharma, Ankur / Vaghasiya, Kalpesh / Ray, Eupa / Gupta, Pushpa / Kumar Singh, Amit / Datta Gupta, Umesh / Kumar Verma, Rahul

International journal of pharmaceutics

2019 Volume 558, Page(s) 231–241

Abstract: Resistance to anti-Tuberculosis (anti-TB) drugs is primarily due to unique intrinsic resistance mechanisms that mycobacterium possess. The most important determinant of resistance is a peculiar hydrophobic and multi-layered mycobacterial cell-wall ... ...

Abstract	Resistance to anti-Tuberculosis (anti-TB) drugs is primarily due to unique intrinsic resistance mechanisms that mycobacterium possess. The most important determinant of resistance is a peculiar hydrophobic and multi-layered mycobacterial cell-wall structure with mycolic-acid and wax-D, which restricts permeability of both hydrophobic and hydrophilic drugs into bacteria. In this study, it was supposed that Host Defense peptides (HDP) which are known to permeabilize bacterial membranes may, therefore, help anti-TB antibiotics to target internal sites in bacteria. To test this hypothesis, we examined the effect of suboptimal concentration (10 µg/ml) of selected microencapsulated-HDP (Ub2-MS, K4-MS, and Aurein1.2-MS) with a standard anti-TB drug (Isoniazid, INH, 3 µg/ml). We also examined the combined effect of different concentrations of HDP-MS with a suboptimal concentration of anti-TB drug (INH, 1.5 µg/ml) which showed additive efficacy. A number of cationic HDP were encapsulated in inhalable microspheres (HDP-MS) and characterized for physicochemical and aerodynamic properties. These peptides were further evaluated for molecular mass by MALDI-TOF and random coil in its secondary structure as determined by circular dichroism. The anti-mycobacterial kinetics of selected HDP-MS (Ub2-MS, K4-MS, and Aurein1.2-MS) was evaluated against virulent Mycobacterium tuberculosis (Mtb), both alone and in conjunction with anti-TB drug (INH). HDP-MS exhibited up to ∼3.02 and ∼3.41-log decrease in CFU as compared to blank-MS (drug free) and untreated control group in 96 h. The combination of HDP-MS with a suboptimal concentration of INH (1.5 µg/ml) showed superior antibiotic activity against Mtb. Our findings show that the enhanced efficacy is due to augmentation of membrane permeation by HDP which expedited the entry of TB drug into apparently the impermeant mycobacterial membrane which further enhances the effective efficacy of the drug. This phenomenon can reduce the need for high dosages and represents a novel paradigm for potential clinical applications.
MeSH term(s)	Animals ; Antitubercular Agents/administration & dosage ; Cell Membrane Permeability/drug effects ; Drug Delivery Systems ; Isoniazid/administration & dosage ; Mice ; Microspheres ; Mycobacterium tuberculosis/drug effects ; Peptides/administration & dosage ; Polylactic Acid-Polyglycolic Acid Copolymer/administration & dosage ; RAW 264.7 Cells ; Tuberculosis/drug therapy
Chemical Substances	Antitubercular Agents ; Peptides ; Polylactic Acid-Polyglycolic Acid Copolymer (1SIA8062RS) ; Isoniazid (V83O1VOZ8L)
Language	English
Publishing date	2019-01-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2018.12.076
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