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  1. Article: Comparison between carbohydrate and salt-based macromolecular crowders for cell preservation at higher temperatures.

    Kumaran, Akash / Bhagwat, Advait / Jain, Ratnesh / Dandekar, Prajakta

    3 Biotech

    2023  Volume 13, Issue 6, Page(s) 184

    Abstract: In this investigation, the macromolecular crowding effect of a carbohydrate-based polymer, pullulan, and a salt-based polymer, poly-(4-styrenesulfonic-acid) sodium salt (PSS) was compared for the storage of A549 lung carcinoma cells, at temperatures ... ...

    Abstract In this investigation, the macromolecular crowding effect of a carbohydrate-based polymer, pullulan, and a salt-based polymer, poly-(4-styrenesulfonic-acid) sodium salt (PSS) was compared for the storage of A549 lung carcinoma cells, at temperatures greater than that of liquid nitrogen storage tanks. A DoE-CCD response surface model was used to optimise medium compositions comprising DMSO and a macromolecular crowder (MMC; pullulan, PSS and their combinations). The effect of adding MMCs was evaluated in terms of post-preservation viability, apoptotic population and growth curve analysis. The optimised medium consisting of 10% DMSO and 3% pullulan in the basal medium (BM) could facilitate long-term cell preservation for 90 days at - 80 °C
    Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03571-6.
    Language English
    Publishing date 2023-05-13
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-023-03571-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cell-based Therapies for Corneal and Retinal Disorders.

    Ajgaonkar, Bhargavi Suryakant / Kumaran, Akash / Kumar, Salil / Jain, Ratnesh D / Dandekar, Prajakta P

    Stem cell reviews and reports

    2023  Volume 19, Issue 8, Page(s) 2650–2682

    Abstract: Maintenance of the visual function is the desired outcome of ophthalmologic therapies. The shortcomings of the current treatment options, like partial recovery, post-operation failure, rigorous post-operative care, complications, etc., which are usually ... ...

    Abstract Maintenance of the visual function is the desired outcome of ophthalmologic therapies. The shortcomings of the current treatment options, like partial recovery, post-operation failure, rigorous post-operative care, complications, etc., which are usually encountered with the conventional treatment options has warranted newer treatment options that may eliminate the root cause of diseases and minimize the side effects. Cell therapies, a class of regenerative medicines, have emerged as cutting-edge treatment option. The corneal and retinal dystrophies during the ocular disorders are the major cause of blindness, worldwide. Corneal disorders are mainly categorized mainly into corneal epithelial, stromal, and endothelial disorders. On the other hand, glaucoma, retinitis pigmentosa, age-related macular degeneration, diabetic retinopathy, Stargardt Disease, choroideremia, Leber congenital amaurosis are then major retinal degenerative disorders. In this manuscript, we have presented a detailed overview of the development of cell-based therapies, using embryonic stem cells, bone marrow stem cells, mesenchymal stem cells, dental pulp stem cells, induced pluripotent stem cells, limbal stem cells, corneal epithelial, stromal and endothelial, embryonic stem cell-derived differentiated cells (like retinal pigment epithelium or RPE), neural progenitor cells, photoreceptor precursors, and bone marrow-derived hematopoietic stem/progenitor cells etc. The manuscript highlights their efficiency, drawbacks and the strategies that have been explored to regain visual function in the preclinical and clinical state associated with them which can be considered for their potential application in the development of treatment.
    MeSH term(s) Humans ; Cornea ; Cell- and Tissue-Based Therapy ; Retinal Degeneration ; Retina ; Corneal Diseases/therapy
    Language English
    Publishing date 2023-09-14
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2495577-2
    ISSN 2629-3277 ; 1558-6804 ; 1550-8943
    ISSN (online) 2629-3277 ; 1558-6804
    ISSN 1550-8943
    DOI 10.1007/s12015-023-10623-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6198: Show issues Location:
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  3. Article ; Online: Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application.

    Kumaran, Akash / Jude Serpes, Nathan / Gupta, Tisha / James, Abija / Sharma, Avinash / Kumar, Deepak / Nagraik, Rupak / Kumar, Vaneet / Pandey, Sadanand

    Biosensors

    2023  Volume 13, Issue 2

    Abstract: With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to ... ...

    Abstract With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are selective, sensitive, flexible to terrain changes, and cost-effective to assist in point-of-care systems for large-scale screening and to be used in remote locations in cases of outbreaks and pandemics. CRISPR-based biosensors comprise a promising new approach to nucleic acid detection, which uses Cas effector proteins (Cas9, Cas12, and Cas13) as extremely specialized identification components that may be used in conjunction with a variety of readout approaches (such as fluorescence, colorimetry, potentiometry, lateral flow assay, etc.) for onsite analysis. In this review, we cover some technical aspects of integrating the CRISPR Cas system with traditional biosensing readout methods and amplification technologies such as polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and recombinase polymerase amplification (RPA) and continue to elaborate on the prospects of the developed biosensor in the detection of some major viral and bacterial diseases. Within the scope of this article, we also discuss the recent COVID pandemic and the numerous CRISPR biosensors that have undergone development since its advent. Finally, we discuss some challenges and future prospects of CRISPR Cas systems in point-of-care testing.
    MeSH term(s) Humans ; Point-of-Care Systems ; COVID-19 ; Point-of-Care Testing ; Biological Assay ; Biosensing Techniques ; Nucleic Acids ; Nucleic Acid Amplification Techniques ; COVID-19 Testing
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2023-01-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios13020202
    Database MEDical Literature Analysis and Retrieval System OnLINE

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