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  1. Article: The Pressure Dependence of the Stability of the G-quadruplex Formed by d(TGGGGT).

    Tariq, Nabeel / Kume, Takuma / Feroze, Ujala N / Macgregor, Robert B

    Life (Basel, Switzerland)

    2022  Volume 12, Issue 5

    Abstract: The G-quadruplex (GQ), a tetrahelix formed by guanine-rich nucleic acid sequences, is a potential drug target for several diseases. Monomolecular GQs are stabilized by guanine tetrads and non-guanine regions that form loops. Hydrostatic pressure ... ...

    Abstract The G-quadruplex (GQ), a tetrahelix formed by guanine-rich nucleic acid sequences, is a potential drug target for several diseases. Monomolecular GQs are stabilized by guanine tetrads and non-guanine regions that form loops. Hydrostatic pressure destabilizes the folded, monomolecular GQ structures. In this communication, we present data on the effect of pressure on the conformational stability of the tetramolecular GQ, d[5'-TGGGGT-3']
    Language English
    Publishing date 2022-05-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life12050765
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Enhancement of the thermal stability of G-quadruplex structures by urea.

    Tariq, Nabeel / Xu, Christine / Wang, Jingtong / Kume, Takuma / Macgregor, Robert B

    Biophysical chemistry

    2023  Volume 299, Page(s) 107043

    Abstract: The solute urea has been used extensively as a denaturant in protein folding studies; double-stranded nucleic acid structures are also destabilized by urea, but comparatively less than proteins. In previous research, the solute has been shown to strongly ...

    Abstract The solute urea has been used extensively as a denaturant in protein folding studies; double-stranded nucleic acid structures are also destabilized by urea, but comparatively less than proteins. In previous research, the solute has been shown to strongly destabilize folded G-quadruplex DNA structures. This contribution demonstrates the stabilizing effect of urea on the G-quadruplex formed by the oligodeoxyribonucleotide (ODN), G3T (d[5'-GGGTGGGTGGGTGGG-3']), and related sequences in the presence of sodium or potassium cations. Stabilization is observed up to 7 M urea, which was the highest concentration we investigated. The folded structure of G3T has three G-tetrads and three loops that consist of single thymine residues. ODNs related to G3T, in which the thymine residues in the loop are substituted by adenosine residues, also exhibit enhanced stability in the presence of molar concentrations of urea. The circular dichroism (CD) spectra of these ODNs in the presence of urea are consistent with that of a G-quadruplex. As the urea concentration increases, the spectral intensities of the peaks and troughs change, while their positions change very little. The heat-induced transition from the folded to unfolded state, T
    MeSH term(s) G-Quadruplexes ; Urea ; Thymine/chemistry ; Thermodynamics ; DNA/chemistry ; Circular Dichroism ; Nucleic Acid Conformation
    Chemical Substances Urea (8W8T17847W) ; Thymine (QR26YLT7LT) ; DNA (9007-49-2)
    Language English
    Publishing date 2023-05-27
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 185052-0
    ISSN 1873-4200 ; 0301-4622
    ISSN (online) 1873-4200
    ISSN 0301-4622
    DOI 10.1016/j.bpc.2023.107043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1147: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Dimethyl sulfoxide (DMSO) is a stabilizing co-solvent for G-quadruplex DNA.

    Tariq, Nabeel / Kume, Takuma / Luo, Lingyan / Cai, Ziwei / Dong, Sihan / Macgregor, Robert B

    Biophysical chemistry

    2022  Volume 282, Page(s) 106741

    Abstract: We report the effect of dimethyl sulfoxide (DMSO) on the stability of the four-stranded structures formed by the oligodeoxyribonucleotides d[5'-AGGG(TTAGGG) ...

    Abstract We report the effect of dimethyl sulfoxide (DMSO) on the stability of the four-stranded structures formed by the oligodeoxyribonucleotides d[5'-AGGG(TTAGGG)
    MeSH term(s) DNA/chemistry ; Dimethyl Sulfoxide ; G-Quadruplexes ; Nucleic Acid Conformation ; Solvents ; Urea/chemistry
    Chemical Substances Solvents ; Urea (8W8T17847W) ; DNA (9007-49-2) ; Dimethyl Sulfoxide (YOW8V9698H)
    Language English
    Publishing date 2022-01-10
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 185052-0
    ISSN 1873-4200 ; 0301-4622
    ISSN (online) 1873-4200
    ISSN 0301-4622
    DOI 10.1016/j.bpc.2021.106741
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1147: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Volumetric Interplay between the Conformational States Adopted by Guanine-Rich DNA from the c-MYC Promoter

    Liu, Lutan / Scott, Lily / Tariq, Nabeel / Kume, Takuma / Dubins, David N. / Macgregor, Robert B. / Chalikian, Tigran V.

    Journal of physical chemistry. 2021 June 29, v. 125, no. 27

    2021  

    Abstract: The kinetic and thermodynamic stabilities of G-quadruplex structures have been extensively studied. In contrast, systematic investigations of the volumetric properties of G-quadruplexes determining their pressure stability are still relatively scarce. ... ...

    Abstract The kinetic and thermodynamic stabilities of G-quadruplex structures have been extensively studied. In contrast, systematic investigations of the volumetric properties of G-quadruplexes determining their pressure stability are still relatively scarce. The G-rich strand from the promoter region of the c-MYC oncogene (G-strand) is known to adopt a range of conformational states including the duplex, G-quadruplex, and coil states depending on the presence of the complementary C-rich strand (C-strand) and solution conditions. In this work, we report changes in volume, ΔV, and adiabatic compressibility, ΔKS, accompanying interconversions of G-strand between the G-quadruplex, duplex, and coil conformations in the presence and absence of C-strand. We rationalize these volumetric characteristics in terms of the hydration and intrinsic properties of the DNA in each of the sampled conformational states. We further use our volumetric results in conjunction with the reported data on changes in expansibility, ΔE, and heat capacity, ΔCP, associated with G-quadruplex-to-coil transitions to construct the pressure–temperature phase diagram describing the stability of the G-quadruplex. The phase diagram is elliptic in shape, resembling the classical elliptic phase diagram of a globular protein, and is distinct from the phase diagram for duplex DNA. The observed similarity of the pressure–temperature phase diagrams of G-quadruplexes and globular proteins stems from their shared structural and hydration features that, in turn, result in the similarity of their volumetric properties. To the best of our knowledge, this is the first pressure–temperature stability diagram reported for a G-quadruplex.
    Keywords DNA ; compressibility ; heat ; oncogenes ; physical chemistry ; physical phases ; promoter regions
    Language English
    Dates of publication 2021-0629
    Size p. 7406-7416.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1520-5207
    DOI 10.1021/acs.jpcb.1c04075
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Volumetric Interplay between the Conformational States Adopted by Guanine-Rich DNA from the c-MYC Promoter.

    Liu, Lutan / Scott, Lily / Tariq, Nabeel / Kume, Takuma / Dubins, David N / Macgregor, Robert B / Chalikian, Tigran V

    The journal of physical chemistry. B

    2021  Volume 125, Issue 27, Page(s) 7406–7416

    Abstract: The kinetic and thermodynamic stabilities of G-quadruplex structures have been extensively studied. In contrast, systematic investigations of the volumetric properties of G-quadruplexes determining their pressure stability are still relatively scarce. ... ...

    Abstract The kinetic and thermodynamic stabilities of G-quadruplex structures have been extensively studied. In contrast, systematic investigations of the volumetric properties of G-quadruplexes determining their pressure stability are still relatively scarce. The G-rich strand from the promoter region of the c-MYC oncogene (G-strand) is known to adopt a range of conformational states including the duplex, G-quadruplex, and coil states depending on the presence of the complementary C-rich strand (C-strand) and solution conditions. In this work, we report changes in volume, Δ
    MeSH term(s) DNA/genetics ; G-Quadruplexes ; Guanine ; Nucleic Acid Conformation ; Promoter Regions, Genetic
    Chemical Substances Guanine (5Z93L87A1R) ; DNA (9007-49-2)
    Language English
    Publishing date 2021-06-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-5207
    ISSN (online) 1520-5207
    DOI 10.1021/acs.jpcb.1c04075
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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