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  1. Article ; Online: Plant metacaspases: Decoding their dynamics in development and disease.

    Basak, Shrabani / Kundu, Pallob

    Plant physiology and biochemistry : PPB

    2022  Volume 180, Page(s) 50–63

    Abstract: Plant metacaspases were evolved in parallel to well-characterized animal counterpart caspases and retained the similar histidine-cysteine catalytic dyad, leading to functional congruity between these endopeptidases. Although phylogenetic relatedness of ... ...

    Abstract Plant metacaspases were evolved in parallel to well-characterized animal counterpart caspases and retained the similar histidine-cysteine catalytic dyad, leading to functional congruity between these endopeptidases. Although phylogenetic relatedness of the catalytic domain and functional commonality placed these proteases in the caspase family, credible counterarguments predominantly about their distinct substrate specificity raised doubts about the classification. Metacaspases are involved in regulating the PCD during development as well as in senescence. Balancing acts of metacaspase activity also dictate cell fate during defense upon the perception of adverse environmental cues. Accordingly, their activity is tightly regulated, while suppressing spurious activation, by a combination of genetic and post-translational modifications. Structural insights from recent studies provided vital clues on the functionality. This comprehensive review aims to explore the origin of plant metacaspases, and their regulatory and functional diversity in different plants while discussing their analogy to mammalian caspases. Besides, we have presented various modern methodologies for analyzing the proteolytic activity of these indispensable molecules in the healthy or stressed life of a plant. The review would serve as a repository of all the available pieces of evidence indicating metacaspases as the key regulator of PCD across the plant kingdom and highlight the prospect of studying metacaspases for their inclusion in a crop improvement program.
    Language English
    Publishing date 2022-03-24
    Publishing country France
    Document type Journal Article ; Review
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2022.03.024
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  2. Article: Plant metacaspases: Decoding their dynamics in development and disease

    Basak, Shrabani / Kundu, Pallob

    Plant physiology and biochemistry. 2022 Mar. 20,

    2022  

    Abstract: Plant metacaspases were evolved in parallel to well-characterized animal counterpart caspases and retained the similar histidine-cysteine catalytic dyad, leading to functional congruity between these endopeptidases. Although phylogenetic relatedness of ... ...

    Abstract Plant metacaspases were evolved in parallel to well-characterized animal counterpart caspases and retained the similar histidine-cysteine catalytic dyad, leading to functional congruity between these endopeptidases. Although phylogenetic relatedness of the catalytic domain and functional commonality placed these proteases in the caspase family, credible counterarguments predominantly about their distinct substrate specificity raised doubts about the classification. Metacaspases are involved in regulating the PCD during development as well as in senescence. Balancing acts of metacaspase activity also dictate cell fate during defense upon the perception of adverse environmental cues. Accordingly, their activity is tightly regulated, while suppressing spurious activation, by a combination of genetic and post-translational modifications. Structural insights from recent studies provided vital clues on the functionality. This comprehensive review aims to explore the origin of plant metacaspases, and their regulatory and functional diversity in different plants while discussing their analogy to mammalian caspases. Besides, we have presented various modern methodologies for analyzing the proteolytic activity of these indispensable molecules in the healthy or stressed life of a plant. The review would serve as a repository of all the available pieces of evidence indicating metacaspases as the key regulator of PCD across the plant kingdom and highlight the prospect of studying metacaspases for their inclusion in a crop improvement program.
    Keywords active sites ; caspases ; functional diversity ; mammals ; phylogeny ; plant physiology ; proteolysis ; substrate specificity
    Language English
    Dates of publication 2022-0320
    Publishing place Elsevier Masson SAS
    Document type Article
    Note Pre-press version
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2022.03.024
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article: Plant miRNA responses under temperature stress

    Das, Rohit / Mukherjee, Ananya / Basak, Shrabani / Kundu, Pallob

    Plant gene. 2021 Dec., v. 28

    2021  

    Abstract: miRNAs are 20–22 nucleotide long regulatory RNA molecules, that are at the forefront of post-transcriptional gene regulation in plants under a plethora of biotic and abiotic stress conditions. Research on miRNA and target gene interactions during stress ... ...

    Abstract miRNAs are 20–22 nucleotide long regulatory RNA molecules, that are at the forefront of post-transcriptional gene regulation in plants under a plethora of biotic and abiotic stress conditions. Research on miRNA and target gene interactions during stress conditions in crop plants have significantly increased in the last decade owing to the availability of genome sequences and access to economical and fast next generation sequencing techniques. This allows scientists a broad but necessary insight into specific regulatory modules that are forefront of stress responsive gene regulation. The changes in the global climate patterns presents us with extreme weather conditions, where the daily minimum and maximum temperatures in many regions having arable lands are reaching never seen extremes. This results in exposure of the crops to temperature fluctuations and resultant stress conditions that were not previously observed. In this context, we have in this review, presented a concise version of the information on miRNA and their target gene modulation under temperature stress conditions, in specific crop plants, that has been unravelled through these sequencing techniques.
    Keywords abiotic stress ; climate ; genes ; microRNA ; temperature ; weather
    Language English
    Dates of publication 2021-12
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 2352-4073
    DOI 10.1016/j.plgene.2021.100317
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  4. Article: Disruption of tomato TGS machinery by ToLCNDV causes reprogramming of vascular tissue-specific TORNADO1 gene expression

    Chowdhury, Shreya / Mukherjee, Ananya / Basak, Shrabani / Das, Rohit / Mandal, Arunava / Kundu, Pallob

    Planta. 2022 Oct., v. 256, no. 4

    2022  

    Abstract: MAIN CONCLUSION: Vascular development-related TRN1 transcription is suppressed by cytosine methylation in fully developed leaves of tomato. ToLCNDV infection disrupts methylation machinery and reactivates TRN1 expression - likely causing abnormal leaf ... ...

    Abstract MAIN CONCLUSION: Vascular development-related TRN1 transcription is suppressed by cytosine methylation in fully developed leaves of tomato. ToLCNDV infection disrupts methylation machinery and reactivates TRN1 expression - likely causing abnormal leaf growth pattern. Leaf curl disease of tomato caused by tomato leaf curl New Delhi virus (ToLCNDV) inflicts huge economical loss. Disease symptoms resemble leaf developmental defects including abnormal vein architecture. Leaf vein patterning-related TORNADO1 gene’s (SlTRN1) transcript level is augmented in virus-infected leaves. To elucidate the molecular mechanism of the upregulation of SlTRN1 in vivo, we have deployed SlTRN1 promoter-reporter transgenic tomato plants and investigated the gene’s dynamic expression pattern in leaf growth stages and infection. Expression of the gene was delimited in the vascular tissues and suppressed in fully developed leaves. WRKY16 transcription factor readily activated SlTRN1 promoter in varied sized leaves and upon virus infection, while silencing of WRKY16 gene resulted in dampened promoter activity. Methylation-sensitive PCR analyses confirmed the accumulation of CHH methylation at multiple locations in the SlTRN1 promoter in older leaves. However, ToLCNDV infection reverses the methylation status and restores expression level in the leaf vascular bundle. The virus dampens the level of key maintenance and de novo DNA methyltransferases SlDRM5, SlMET1, SlCMT2 with concomitant augmentation of two DNA demethylases, SlDML1 and SlDML2 levels in SlTRN1 promoter-reporter transgenics. Transient overexpression of SlDML2 mimics the virus-induced hypomethylation state of the SlTRN1 promoter in mature leaves, while silencing of SlDML2 lessens promoter activity. Furthermore, in line with the previous studies, we confirm the crucial role of viral suppressors of RNA silencing AC2 and AC4 proteins in promoting DNA demethylation and directing it to restore activated transcription of SlTRN1. Unusually elevated expression of SlTRN1 may negatively impact normal growth of leaves.
    Keywords DNA ; DNA demethylation ; DNA methylation ; DNA methyltransferase ; RNA ; Tomato leaf curl New Delhi virus ; gene expression ; genes ; genetically modified organisms ; leaf curling ; leaves ; tomatoes ; transcription factors ; viruses
    Language English
    Dates of publication 2022-10
    Size p. 78.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/s00425-022-03985-1
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  5. Article: Heightened miR6024-NLR interactions facilitate necrotrophic pathogenesis in tomato

    Dey, Sayani / Sarkar, Arijita / Chowdhury, Shreya / Singh, Raghuvir / Mukherjee, Ananya / Ghosh, Zhumur / Kundu, Pallob

    Plant molecular biology. 2022 Aug., v. 109, no. 6

    2022  

    Abstract: KEY MESSAGE: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral ... ...

    Abstract KEY MESSAGE: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral components of plant disease stress-signaling are targeted by variable groups of miRNAs. However, the significance of miRNA-mediated regulation of NLRs during a pathogen stress response, specifically for necrotrophic fungus, is poorly understood. A thorough examination of Tomato NLRs and miRNAs could map substantial interactions of which half the annotated NLRs were targets of Solanaceae-specific and conserved miRNAs, at the NB subdomain. The Solanaceae-specific miR6024 and its NLR targets analysed in different phytopathogenic stresses revealed differential and mutually antagonistic regulation. Interestingly, miR6024-targeted cleavage of a target NLR also triggered the generation of secondary phased siRNAs which could potentially amplify the defense signal. RNA-seq analysis of leaf tissues from miR6024 overexpressing Tomato plants evidenced a perturbation in the defense transcriptome with the transgenics showing unwarranted immune response-related genes’ expression with or without infection with necrotrophic Alternaria solani, though no adverse effect could be observed in the growth and development of the transgenic plants. Transgenic plants exhibited constitutive downregulation of the target NLRs, aggravated disease phenotype with an enhanced lesion, greater ROS generation and hypersusceptibility to A. solani infection, thus establishing that miR6024 negatively impacts plant immune response during necrotrophic pathogenesis. Limited knowledge about the outcome of NLR-miRNA interaction during necrotrophic pathogenesis is a hindrance to the deployment of miRNAs in crop improvement programs. With the elucidation of the necrotrophic disease-synergistic role played by miR6024, it becomes a potent candidate for biotechnological manipulation for the rapid development of pathogen-tolerant solanaceous plants.
    Keywords Alternaria solani ; adverse effects ; fungi ; growth and development ; immune response ; leaves ; microRNA ; molecular biology ; pathogenesis ; pathogens ; phenotype ; sequence analysis ; stress response ; tomatoes ; transcriptome
    Language English
    Dates of publication 2022-08
    Size p. 717-739.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-022-01270-z
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    Einzelsign.: Show issues
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  6. Article ; Online: Disruption of tomato TGS machinery by ToLCNDV causes reprogramming of vascular tissue-specific TORNADO1 gene expression.

    Chowdhury, Shreya / Mukherjee, Ananya / Basak, Shrabani / Das, Rohit / Mandal, Arunava / Kundu, Pallob

    Planta

    2022  Volume 256, Issue 4, Page(s) 78

    Abstract: Main conclusion: Vascular development-related TRN1 transcription is suppressed by cytosine methylation in fully developed leaves of tomato. ToLCNDV infection disrupts methylation machinery and reactivates TRN1 expression - likely causing abnormal leaf ... ...

    Abstract Main conclusion: Vascular development-related TRN1 transcription is suppressed by cytosine methylation in fully developed leaves of tomato. ToLCNDV infection disrupts methylation machinery and reactivates TRN1 expression - likely causing abnormal leaf growth pattern. Leaf curl disease of tomato caused by tomato leaf curl New Delhi virus (ToLCNDV) inflicts huge economical loss. Disease symptoms resemble leaf developmental defects including abnormal vein architecture. Leaf vein patterning-related TORNADO1 gene's (SlTRN1) transcript level is augmented in virus-infected leaves. To elucidate the molecular mechanism of the upregulation of SlTRN1 in vivo, we have deployed SlTRN1 promoter-reporter transgenic tomato plants and investigated the gene's dynamic expression pattern in leaf growth stages and infection. Expression of the gene was delimited in the vascular tissues and suppressed in fully developed leaves. WRKY16 transcription factor readily activated SlTRN1 promoter in varied sized leaves and upon virus infection, while silencing of WRKY16 gene resulted in dampened promoter activity. Methylation-sensitive PCR analyses confirmed the accumulation of CHH methylation at multiple locations in the SlTRN1 promoter in older leaves. However, ToLCNDV infection reverses the methylation status and restores expression level in the leaf vascular bundle. The virus dampens the level of key maintenance and de novo DNA methyltransferases SlDRM5, SlMET1, SlCMT2 with concomitant augmentation of two DNA demethylases, SlDML1 and SlDML2 levels in SlTRN1 promoter-reporter transgenics. Transient overexpression of SlDML2 mimics the virus-induced hypomethylation state of the SlTRN1 promoter in mature leaves, while silencing of SlDML2 lessens promoter activity. Furthermore, in line with the previous studies, we confirm the crucial role of viral suppressors of RNA silencing AC2 and AC4 proteins in promoting DNA demethylation and directing it to restore activated transcription of SlTRN1. Unusually elevated expression of SlTRN1 may negatively impact normal growth of leaves.
    MeSH term(s) Begomovirus/genetics ; Gene Expression ; Lycopersicon esculentum/genetics ; Plant Diseases/genetics ; Plants, Genetically Modified/genetics
    Language English
    Publishing date 2022-09-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/s00425-022-03985-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Bonn / Germany

    Z 46/303: Show issues

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  7. Article ; Online: Heightened miR6024-NLR interactions facilitate necrotrophic pathogenesis in tomato.

    Dey, Sayani / Sarkar, Arijita / Chowdhury, Shreya / Singh, Raghuvir / Mukherjee, Ananya / Ghosh, Zhumur / Kundu, Pallob

    Plant molecular biology

    2022  Volume 109, Issue 6, Page(s) 717–739

    Abstract: Key message: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral ... ...

    Abstract Key message: miR6024 acts as a negative regulator of R genes, hence of Tomato plant immunity, and facilitates disease by the necrotrophic pathogen A. solani. Plant resistance genes or Nucleotide-binding leucine-rich repeat (NLR) genes, integral components of plant disease stress-signaling are targeted by variable groups of miRNAs. However, the significance of miRNA-mediated regulation of NLRs during a pathogen stress response, specifically for necrotrophic fungus, is poorly understood. A thorough examination of Tomato NLRs and miRNAs could map substantial interactions of which half the annotated NLRs were targets of Solanaceae-specific and conserved miRNAs, at the NB subdomain. The Solanaceae-specific miR6024 and its NLR targets analysed in different phytopathogenic stresses revealed differential and mutually antagonistic regulation. Interestingly, miR6024-targeted cleavage of a target NLR also triggered the generation of secondary phased siRNAs which could potentially amplify the defense signal. RNA-seq analysis of leaf tissues from miR6024 overexpressing Tomato plants evidenced a perturbation in the defense transcriptome with the transgenics showing unwarranted immune response-related genes' expression with or without infection with necrotrophic Alternaria solani, though no adverse effect could be observed in the growth and development of the transgenic plants. Transgenic plants exhibited constitutive downregulation of the target NLRs, aggravated disease phenotype with an enhanced lesion, greater ROS generation and hypersusceptibility to A. solani infection, thus establishing that miR6024 negatively impacts plant immune response during necrotrophic pathogenesis. Limited knowledge about the outcome of NLR-miRNA interaction during necrotrophic pathogenesis is a hindrance to the deployment of miRNAs in crop improvement programs. With the elucidation of the necrotrophic disease-synergistic role played by miR6024, it becomes a potent candidate for biotechnological manipulation for the rapid development of pathogen-tolerant solanaceous plants.
    MeSH term(s) Lycopersicon esculentum/microbiology ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plant Immunity ; Plants, Genetically Modified/genetics
    Chemical Substances MicroRNAs
    Language English
    Publishing date 2022-05-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-022-01270-z
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3458: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Method to Study Dynamics of Membrane-Bound Plant Transcription Factors During Biotic Interactions in Tomato.

    Chowdhury, Supriyo / Bhattacharjee, Payel / Basak, Shrabani / Chowdhury, Shreya / Kundu, Pallob

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 1991, Page(s) 61–68

    Abstract: Sequestration of a transcription factor in a cellular membrane and releasing it on demand is an additional layer of gene regulation that is considered a rapid mode to reprogram a gene expression cascade when a plasma membrane stress signal is perceived. ... ...

    Abstract Sequestration of a transcription factor in a cellular membrane and releasing it on demand is an additional layer of gene regulation that is considered a rapid mode to reprogram a gene expression cascade when a plasma membrane stress signal is perceived. Better understanding of the dynamic exchange of membrane-bound transcription factors (MTFs) during biotic stress requires the development of a simple, efficient, and quick assay system. Here we report an Agrobacterium-based transient transformation method to assay the localization of fluorescent protein-tagged MTFs in tomato leaf epidermal peels that are subsequently infected with a pathogenic fungus. Essentially, our method mimics natural infection and facilitates the realistic monitoring of MTF movement during activation of a signaling event.
    MeSH term(s) Agrobacterium/physiology ; Cell Membrane/metabolism ; Lycopersicon esculentum/genetics ; Lycopersicon esculentum/metabolism ; Lycopersicon esculentum/microbiology ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Leaves/microbiology ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Stress, Physiological ; Transcription Factors/genetics ; Transcription Factors/metabolism
    Chemical Substances Membrane Proteins ; Plant Proteins ; Transcription Factors
    Language English
    Publishing date 2019-04-30
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9458-8_7
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  9. Article ; Online: Inhibition of Bemisia tabaci vectored, GroEL mediated transmission of tomato leaf curl New Delhi virus by garlic leaf lectin (Allium sativum leaf agglutinin).

    Das, Ayan / Roy, Amit / Mandal, Arunava / Mondal, Hossian Ali / Hess, Daniel / Kundu, Pallob / Das, Sampa

    Virus research

    2021  Volume 300, Page(s) 198443

    Abstract: GroEL or symbionin synthesized by the endosymbionts of whitefly (Bemisia tabaci)/ aphids play a cardinal role in the persistent, circulative transmission of plant viruses by binding to viral coat protein/ read-through protein. Allium sativum leaf ... ...

    Abstract GroEL or symbionin synthesized by the endosymbionts of whitefly (Bemisia tabaci)/ aphids play a cardinal role in the persistent, circulative transmission of plant viruses by binding to viral coat protein/ read-through protein. Allium sativum leaf agglutinin (ASAL), a Galanthus nivalis agglutinin (GNA)- related mannose-binding lectin from garlic leaf has been reported as a potent controlling agent against hemipteran insects including whitefly and aphids. GroEL related chaperonin- symbionin was previously identified as a receptor of ASAL by the present group in the brush border membrane vesicle (BBMV) of mustard aphid. In the present study similar GroEL receptor of ASAL has been identified through LC-MS/MS in the BBMV of B. tabaci which serves as a vector for several plant viruses including tomato leaf curl New Delhi virus (ToLCNDV). Ligand blot analysis of ASAL-fed B. tabaci showed that when GroEL is pre-occupied by ASAL, it completely blocks its further binding to ToLCNDV coat protein (ToLCNDV-CP). Prior feeding of ASAL hindered the co-localization of ToLCNDV-CP and GroEL in the midgut of B. tabaci. Immunoprecipitation followed by western blot with ASAL-fed B. tabaci yielded similar result. Moreover, ASAL feeding inhibited viral transmission by B. tabaci. Together, these results confirmed that the interaction of ASAL with GroEL interferes with the binding of ToLCNDV-CP and inhibits further B. tabaci mediated viral transmission.
    MeSH term(s) Agglutinins ; Animals ; Aphids ; Begomovirus/genetics ; Chromatography, Liquid ; Garlic ; Hemiptera ; Lectins ; Plant Diseases ; Tandem Mass Spectrometry
    Chemical Substances Agglutinins ; Lectins
    Language English
    Publishing date 2021-04-30
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2021.198443
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1965: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  10. Article ; Online: Functional characterization of tomato membrane-bound NAC transcription factors.

    Bhattacharjee, Payel / Das, Rohit / Mandal, Arunava / Kundu, Pallob

    Plant molecular biology

    2016  Volume 93, Issue 4-5, Page(s) 511–532

    Abstract: Key message: Genome-wide analysis was carried out to identify and analyze differential expression pattern of tomato membrane bound NAC transcription factors (SlNACMTFs) during stresses. Two biotic-stress-related SlNACMTFs have been characterized to ... ...

    Abstract Key message: Genome-wide analysis was carried out to identify and analyze differential expression pattern of tomato membrane bound NAC transcription factors (SlNACMTFs) during stresses. Two biotic-stress-related SlNACMTFs have been characterized to elucidate their regulatory function. NAC transcription factors are known regulators of stress-related gene expression. As Stresses are perceived and transmitted by membrane-bound proteins, functional characterization of membrane-associated NAC transcription factors in tomato can reveal valuable insight about membrane-mediated stress-signalling. Tomato genome encodes 13 NAC genes which have predicted transmembrane domain(s) (SlNACMTFs). mRNA of 12 SlNACMTFs were readily detected in multiple tissues, and also in polysome isolated from leaf, confirming active transcription and translation from these genes occur under normal physiological condition. Additionally, most of the SlNACMTFs were differentially regulated during stresses and stress-related transcription factor binding sites are prevalent in their promoters. SlNACMTF3 and 8 were majorly regulated in biotic and abiotic stresses. Like other MTFs, SlNACMTF3 was translocated to the plasma membrane, whereas the C-terminus truncated (ΔC) form localized in the cytoplasm and the nucleus. Accordingly, the ΔC forms significantly influenced the activity of promoters harbouring NAC binding sites (NACbs). Furthermore, the NAC domain of these transcription factors could directly interact with an NACbs, and the proteins failed to regulate a promoter lacking a crucial NACbs. Interestingly, the type of influence to an NACbs containing promoter was dependent on the context of the NACbs, as the same SlNACMTF showed an alternative mode of regulation on different promoters, as well as the same promoter activity was oppositely regulated by two different SlNACMTF. Finally, both SlNACMTFs demonstrated the differential regulatory effect on the expression of several stress-related genes by interacting with the putative NACbs in their promoter region, suggesting their direct role in plant stress response.
    MeSH term(s) Adaptation, Physiological/genetics ; Alternaria/physiology ; Amino Acid Sequence ; Binding Sites/genetics ; Gene Expression Profiling/methods ; Gene Expression Regulation, Plant/drug effects ; Lycopersicon esculentum/genetics ; Lycopersicon esculentum/microbiology ; Membrane Proteins/classification ; Membrane Proteins/genetics ; Phylogeny ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plant Leaves/genetics ; Plant Leaves/microbiology ; Plant Proteins/genetics ; Plants, Genetically Modified ; Protein Binding ; Reverse Transcriptase Polymerase Chain Reaction ; Sodium Chloride/pharmacology ; Temperature ; Transcription Factors/classification ; Transcription Factors/genetics
    Chemical Substances Membrane Proteins ; Plant Proteins ; Transcription Factors ; Sodium Chloride (451W47IQ8X)
    Language English
    Publishing date 2016-12-30
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 778032-1
    ISSN 1573-5028 ; 0167-4412
    ISSN (online) 1573-5028
    ISSN 0167-4412
    DOI 10.1007/s11103-016-0579-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3458: Show issues Location:
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