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  1. Article: Involvement of CYP347W1 in neurotoxin 3‐nitropropionic acid‐based chemical defense in mustard leaf beetle Phaedon cochleariae

    Fu, Nanxia / Becker, Tobias / Brandt, Wolfgang / Kunert, Maritta / Burse, Antje / Boland, Wilhelm

    Insect science. 2022 Apr., v. 29, no. 2

    2022  

    Abstract: Chrysomelina beetlesstore 3‐nitropropionic acid in form of a pretoxin, isoxazolin‐5‐one glucoside‐conjugated ester, to protect themselves against predators. Here we identified a cytochrome P450 monooxygenase, CYP347W1, to be involved in the production of ...

    Abstract Chrysomelina beetlesstore 3‐nitropropionic acid in form of a pretoxin, isoxazolin‐5‐one glucoside‐conjugated ester, to protect themselves against predators. Here we identified a cytochrome P450 monooxygenase, CYP347W1, to be involved in the production of the 3‐nitropropionic acid moiety of the isoxazolin‐5‐one glucoside ester. Knocking down CYP347W1 led to a significant depletion in the concentration of the isoxazolin‐5‐one glucoside ester and an increase in the concentration of the isoxazolin‐5‐one glucoside in the larval hemolymph. Enzyme assays with the heterologously expressed CYP347W1 showed free β‐alanine was not the direct substrate. Homology modeling indicated that β‐alanine‐CoA ester can fit into CYP347W1’s active site. Furthermore, we proved that Phaedon cochleariae eggs are not able to de novo synthesize 3‐NPA, although both isoxazolin‐5‐one glucoside and its 3‐NPA‐conjugated ester are present in the eggs. These results provide direct evidence for the involvement of CYP347W1 in the biosynthesis of a P. cochleariae chemical defense compound.
    Keywords 3-nitropropionic acid ; Phaedon cochleariae ; active sites ; biosynthesis ; chemical defenses ; cytochrome P-450 ; enzymes ; glucosides ; hemolymph ; insects ; larvae ; moieties ; neurotoxins
    Language English
    Dates of publication 2022-04
    Size p. 453-466.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.12944
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Ectomycorrhizal Influence on the Dynamics of Sesquiterpene Release by

    Ezediokpu, Marycolette Ndidi / Krause, Katrin / Kunert, Maritta / Hoffmeister, Dirk / Boland, Wilhelm / Kothe, Erika

    Journal of fungi (Basel, Switzerland)

    2022  Volume 8, Issue 6

    Abstract: Tricholoma ... ...

    Abstract Tricholoma vaccinum
    Language English
    Publishing date 2022-05-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8060555
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  3. Article ; Online: In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria)

    Palmer, Lira / Chuang, Ling / Siegmund, Marlen / Kunert, Maritta / Yamamoto, Kōtarō / Sonawane, Prashant / O’Connor, Sarah E.

    Planta. 2022 Nov., v. 256, no. 5 p.99-99

    2022  

    Abstract: MAIN CONCLUSION: Using virus-induced gene silencing, we demonstrated that the enzymes GES, ISY, and MLPL are responsible for nepetalactone biosynthesis in Nepeta cataria. Nepetalactone is the main iridoid that is found in the Nepeta genus and is well- ... ...

    Abstract MAIN CONCLUSION: Using virus-induced gene silencing, we demonstrated that the enzymes GES, ISY, and MLPL are responsible for nepetalactone biosynthesis in Nepeta cataria. Nepetalactone is the main iridoid that is found in the Nepeta genus and is well-known for its psychoactive effect on house cats. Moreover, there is a burgeoning interest into the effect of nepetalactone on insects. Although the enzymes for nepetalactone biosynthesis have been biochemically assayed in vitro, validation of the role that these enzymes have in planta has not been demonstrated. Virus-induced gene silencing (VIGS) is a silencing method that relies on transient transformation and is an approach that has been particularly successful when applied to a variety of non-model plants. Here, we use a recently designed visual-marker dependent VIGS system to demonstrate that the nepetalactone biosynthetic enzymes GES, ISY, and MLPL impact nepetalactone biosynthesis in Nepeta cataria.
    Keywords Nepeta cataria ; biosynthesis ; genes
    Language English
    Dates of publication 2022-11
    Size p. 99.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/s00425-022-04012-z
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    In stock of ZB MED Bonn / Germany

    Z 46/303: Show issues

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  4. Article ; Online: Function of sesquiterpenes from Schizophyllum commune in interspecific interactions.

    Wirth, Sophia / Krause, Katrin / Kunert, Maritta / Broska, Selina / Paetz, Christian / Boland, Wilhelm / Kothe, Erika

    PloS one

    2021  Volume 16, Issue 1, Page(s) e0245623

    Abstract: Wood is a habitat for a variety of organisms, including saprophytic fungi and bacteria, playing an important role in wood decomposition. Wood inhabiting fungi release a diversity of volatiles used as signaling compounds to attract or repel other ... ...

    Abstract Wood is a habitat for a variety of organisms, including saprophytic fungi and bacteria, playing an important role in wood decomposition. Wood inhabiting fungi release a diversity of volatiles used as signaling compounds to attract or repel other organisms. Here, we show that volatiles of Schizophyllum commune are active against wood-decay fungi and bacteria found in its mycosphere. We identified sesquiterpenes as the biologically active compounds, that inhibit fungal growth and modify bacterial motility. The low number of cultivable wood inhabiting bacteria prompted us to analyze the microbial community in the mycosphere of S. commune using a culture-independent approach. Most bacteria belong to Actinobacteria and Proteobacteria, including Pseudomonadaceae, Sphingomonadaceae, Erwiniaceae, Yersiniaceae and Mariprofundacea as the dominating families. In the fungal community, the phyla of ascomycetes and basidiomycetes were well represented. We propose that fungal volatiles might have an important function in the wood mycosphere and could meditate interactions between microorganisms across domains and within the fungal kingdom.
    MeSH term(s) Actinobacteria/metabolism ; Proteobacteria/metabolism ; Schizophyllum/metabolism ; Sesquiterpenes/metabolism ; Soil Microbiology
    Chemical Substances Sesquiterpenes
    Language English
    Publishing date 2021-01-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0245623
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Involvement of CYP347W1 in neurotoxin 3-nitropropionic acid-based chemical defense in mustard leaf beetle Phaedon cochleariae.

    Fu, Nanxia / Becker, Tobias / Brandt, Wolfgang / Kunert, Maritta / Burse, Antje / Boland, Wilhelm

    Insect science

    2021  Volume 29, Issue 2, Page(s) 453–466

    Abstract: Chrysomelina beetlesstore 3-nitropropionic acid in form of a pretoxin, isoxazolin-5-one glucoside-conjugated ester, to protect themselves against predators. Here we identified a cytochrome P450 monooxygenase, CYP347W1, to be involved in the production of ...

    Abstract Chrysomelina beetlesstore 3-nitropropionic acid in form of a pretoxin, isoxazolin-5-one glucoside-conjugated ester, to protect themselves against predators. Here we identified a cytochrome P450 monooxygenase, CYP347W1, to be involved in the production of the 3-nitropropionic acid moiety of the isoxazolin-5-one glucoside ester. Knocking down CYP347W1 led to a significant depletion in the concentration of the isoxazolin-5-one glucoside ester and an increase in the concentration of the isoxazolin-5-one glucoside in the larval hemolymph. Enzyme assays with the heterologously expressed CYP347W1 showed free β-alanine was not the direct substrate. Homology modeling indicated that β-alanine-CoA ester can fit into CYP347W1's active site. Furthermore, we proved that Phaedon cochleariae eggs are not able to de novo synthesize 3-NPA, although both isoxazolin-5-one glucoside and its 3-NPA-conjugated ester are present in the eggs. These results provide direct evidence for the involvement of CYP347W1 in the biosynthesis of a P. cochleariae chemical defense compound.
    MeSH term(s) Animals ; Coleoptera/chemistry ; Mustard Plant ; Neurotoxins/toxicity ; Nitro Compounds/pharmacology ; Propionates
    Chemical Substances Neurotoxins ; Nitro Compounds ; Propionates ; 3-nitropropionic acid (QY4L0FOX0D)
    Language English
    Publishing date 2021-09-17
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.12944
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria).

    Palmer, Lira / Chuang, Ling / Siegmund, Marlen / Kunert, Maritta / Yamamoto, Kotaro / Sonawane, Prashant / O'Connor, Sarah E

    Planta

    2022  Volume 256, Issue 5, Page(s) 99

    Abstract: Main conclusion: Using virus-induced gene silencing, we demonstrated that the enzymes GES, ISY, and MLPL are responsible for nepetalactone biosynthesis in Nepeta cataria. Nepetalactone is the main iridoid that is found in the Nepeta genus and is well- ... ...

    Abstract Main conclusion: Using virus-induced gene silencing, we demonstrated that the enzymes GES, ISY, and MLPL are responsible for nepetalactone biosynthesis in Nepeta cataria. Nepetalactone is the main iridoid that is found in the Nepeta genus and is well-known for its psychoactive effect on house cats. Moreover, there is a burgeoning interest into the effect of nepetalactone on insects. Although the enzymes for nepetalactone biosynthesis have been biochemically assayed in vitro, validation of the role that these enzymes have in planta has not been demonstrated. Virus-induced gene silencing (VIGS) is a silencing method that relies on transient transformation and is an approach that has been particularly successful when applied to a variety of non-model plants. Here, we use a recently designed visual-marker dependent VIGS system to demonstrate that the nepetalactone biosynthetic enzymes GES, ISY, and MLPL impact nepetalactone biosynthesis in Nepeta cataria.
    MeSH term(s) Cyclopentane Monoterpenes ; Iridoids ; Nepeta/chemistry ; Nepeta/genetics ; Pyrones/chemistry ; Pyrones/pharmacology
    Chemical Substances Cyclopentane Monoterpenes ; Iridoids ; Pyrones ; nepetalactone (21651-62-7)
    Language English
    Publishing date 2022-10-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/s00425-022-04012-z
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    In stock of ZB MED Bonn / Germany

    Z 46/303: Show issues

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  7. Article ; Online: Sexual communication of Spodoptera frugiperda from West Africa: Adaptation of an invasive species and implications for pest management.

    Haenniger, Sabine / Goergen, Georg / Akinbuluma, Mobolade Dele / Kunert, Maritta / Heckel, David G / Unbehend, Melanie

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 2892

    Abstract: The pest species Spodoptera frugiperda, which is native to North and South America, has invaded Africa in 2016. The species consists of two strains, the corn-strain and rice-strain, which differ in their sexual communication. When we investigated ... ...

    Abstract The pest species Spodoptera frugiperda, which is native to North and South America, has invaded Africa in 2016. The species consists of two strains, the corn-strain and rice-strain, which differ in their sexual communication. When we investigated populations from Benin and Nigeria, consisting of corn-strain and rice-corn-hybrid descendants, we found no strain-specific sexual communication differences. Both genotypes exhibited the same pheromone composition, consisting of around 97% (Z)-9-tetradecenyl acetate (Z9-14:Ac), 2% (Z)-7-dodecenyl acetate (Z7-12:Ac), and 1% (Z)-9-dodecenyl acetate (Z9-12:Ac), they had similar electrophysiological responses, and all mated around three hours into scotophase. However, we found geographic variation between African and American populations. The sex pheromone of African corn-strain and hybrid descendant females was similar to American rice-strain females and showed higher percentages of the male-attracting minor component Z7-12:Ac. In addition, African males exhibited the highest antennal sensitivity towards Z7-12:Ac, while American males showed highest sensitivity towards the major pheromone component Z9-14:Ac. Increasing the production of and response to the critical minor component Z7-12:Ac may reduce communication interference with other African Spodoptera species that share the same major pheromone component. The implications of our results on pheromone-based pest management strategies are discussed.
    MeSH term(s) Adaptation, Physiological/drug effects ; Africa, Western ; Animals ; Arthropod Antennae/drug effects ; Arthropod Antennae/physiology ; Dodecanol/analogs & derivatives ; Dodecanol/pharmacology ; Fatty Acids, Monounsaturated/pharmacology ; Female ; Introduced Species ; Male ; Pest Control ; Pheromones/pharmacology ; Sexual Behavior, Animal/drug effects ; Sexual Behavior, Animal/physiology ; Spodoptera/drug effects ; Spodoptera/genetics ; Spodoptera/physiology ; Zea mays
    Chemical Substances 9-tetradecenyl acetate ; Fatty Acids, Monounsaturated ; Pheromones ; 9-dodecenyl acetate (16974-34-8) ; Dodecanol (178A96NLP2)
    Language English
    Publishing date 2020-02-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-59708-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Promiscuous CYP87A enzyme activity initiates cardenolide biosynthesis in plants.

    Kunert, Maritta / Langley, Chloe / Lucier, Rosalind / Ploss, Kerstin / Rodríguez López, Carlos E / Serna Guerrero, Delia A / Rothe, Eva / O'Connor, Sarah E / Sonawane, Prashant D

    Nature plants

    2023  Volume 9, Issue 10, Page(s) 1607–1617

    Abstract: Cardenolides are specialized, steroidal metabolites produced in a wide array of plant ... ...

    Abstract Cardenolides are specialized, steroidal metabolites produced in a wide array of plant families
    MeSH term(s) Cardenolides/metabolism ; Plants/metabolism ; Digitalis/chemistry ; Digitalis/metabolism ; Pregnenolone
    Chemical Substances Cardenolides ; Pregnenolone (73R90F7MQ8)
    Language English
    Publishing date 2023-09-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2055-0278
    ISSN (online) 2055-0278
    DOI 10.1038/s41477-023-01515-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: SpitWorm, a Herbivorous Robot: Mechanical Leaf Wounding with Simultaneous Application of Salivary Components

    Li, Guanjun / Bartram, Stefan / Guo, Huijuan / Mithöfer, Axel / Kunert, Maritta / Boland, Wilhelm

    Plants. 2019 Aug. 31, v. 8, no. 9

    2019  

    Abstract: Induction of jasmonate-mediated plant defense against insect herbivory is initiated by a combination of both mechanical wounding and chemical factors. In order to study both effects independently on plant defense induction, SpitWorm, a computer- ... ...

    Abstract Induction of jasmonate-mediated plant defense against insect herbivory is initiated by a combination of both mechanical wounding and chemical factors. In order to study both effects independently on plant defense induction, SpitWorm, a computer-controlled device which mimics the damage pattern of feeding insect larvae on leaves and, in addition, can apply oral secretions (OS) or other solutions to the ‘biting site’ during ‘feeding,’ was developed and evaluated. The amount of OS left by a Spodoptera littoralis larva during feeding on Phaseolus lunatus (lima bean) leaves was estimated by combining larval foregut volume, biting rate, and quantification of a fluorescent dye injected into the larvae’s foregut prior to feeding. For providing OS amounts by SpitWorm equivalent to larval feeding, dilution and delivery rate were optimized. The effectiveness of SpitWorm was tested by comparing volatile organic compounds (VOC) emissions of P. lunatus leaves treated with either SpitWorm, MecWorm, or S. littoralis larvae. Identification and quantification of emitted VOCs revealed that SpitWorm induced a volatile bouquet that is qualitatively and quantitatively similar to herbivory. Additionally, RT-qPCR of four jasmonic acid responsive genes showed that SpitWorm, in contrast to MecWorm, induces the same regulation pattern as insect feeding. Thus, SpitWorm mimics insect herbivory almost identically to real larvae feeding.
    Keywords Phaseolus lunatus ; Spodoptera littoralis ; emissions ; feeding behavior ; fluorescent dyes ; foregut ; genes ; herbivores ; insect larvae ; insects ; jasmonic acid ; leaves ; lima beans ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; robots ; secretion ; volatile organic compounds
    Language English
    Dates of publication 2019-0831
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants8090318
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: SpitWorm, a Herbivorous Robot: Mechanical Leaf Wounding with Simultaneous Application of Salivary Components.

    Li, Guanjun / Bartram, Stefan / Guo, Huijuan / Mithöfer, Axel / Kunert, Maritta / Boland, Wilhelm

    Plants (Basel, Switzerland)

    2019  Volume 8, Issue 9

    Abstract: Induction of jasmonate-mediated plant defense against insect herbivory is initiated by a combination of both mechanical wounding and chemical factors. In order to study both effects independently on plant defense induction, SpitWorm, a computer- ... ...

    Abstract Induction of jasmonate-mediated plant defense against insect herbivory is initiated by a combination of both mechanical wounding and chemical factors. In order to study both effects independently on plant defense induction, SpitWorm, a computer-controlled device which mimics the damage pattern of feeding insect larvae on leaves and, in addition, can apply oral secretions (OS) or other solutions to the 'biting site' during 'feeding,' was developed and evaluated. The amount of OS left by a
    Language English
    Publishing date 2019-08-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants8090318
    Database MEDical Literature Analysis and Retrieval System OnLINE

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