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  1. Article ; Online: Contribution of a ZIP-family protein to manganese uptake and infective endocarditis virulence in Streptococcus sanguinis.

    Puccio, Tanya / Kunka, Karina S / An, Seon-Sook / Kitten, Todd

    Molecular microbiology

    2021  Volume 117, Issue 2, Page(s) 353–374

    Abstract: Streptococcus sanguinis is an important cause of infective endocarditis. In strain SK36, the ABC-family manganese transporter, SsaACB, is essential for virulence. We have now identified a ZIP-family protein, TmpA, as a secondary manganese transporter. A ... ...

    Abstract Streptococcus sanguinis is an important cause of infective endocarditis. In strain SK36, the ABC-family manganese transporter, SsaACB, is essential for virulence. We have now identified a ZIP-family protein, TmpA, as a secondary manganese transporter. A tmpA mutant had no phenotype, but a ΔssaACB ΔtmpA mutant was more attenuated for serum growth and for virulence in a rabbit model than its ΔssaACB parent. The growth of both mutants was restored by supplemental manganese, but the ΔssaACB ΔtmpA mutant required twenty-fold more and accumulated less. Although ZIP-family proteins are known for zinc and iron transport, TmpA-mediated transport of either metal was minimal. While ssaACB appears ubiquitous in St. sanguinis, tmpA was present in a majority of strains and a mntH gene encoding an NRAMP-family transporter was identified in relatively few, including VMC66. As in SK36, deletion of ssaACB greatly diminished VMC66 endocarditis virulence and serum growth, and deletion of tmpA from this mutant diminished virulence further. Virulence was not significantly altered by deletion of mntH from either VMC66 or its ΔssaACB mutant. This and the accompanying paper together suggest that SsaACB is of primary importance for endocarditis virulence while secondary transporters TmpA and MntH contribute to growth under differing conditions.
    MeSH term(s) Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Endocarditis ; Endocarditis, Bacterial ; Manganese/metabolism ; Rabbits ; Streptococcus sanguis/metabolism ; Virulence
    Chemical Substances Bacterial Proteins ; Manganese (42Z2K6ZL8P)
    Language English
    Publishing date 2021-12-18
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.14853
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Genome Sequence of the Early 20th-Century Extreme Halophile

    DasSarma, Priya / Anton, Brian P / Griffith, Jessie M / Kunka, Karina S / Roberts, Richard J / DasSarma, Shiladitya

    Microbiology resource announcements

    2022  Volume 11, Issue 1, Page(s) e0118121

    Abstract: ... ...

    Abstract Halobacterium
    Language English
    Publishing date 2022-01-13
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.01181-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Manganese Depletion Leads to Multisystem Changes in the Transcriptome of the Opportunistic Pathogen

    Puccio, Tanya / Kunka, Karina S / Zhu, Bin / Xu, Ping / Kitten, Todd

    Frontiers in microbiology

    2020  Volume 11, Page(s) 592615

    Abstract: Streptococcus ... ...

    Abstract Streptococcus sanguinis
    Language English
    Publishing date 2020-11-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.592615
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Acid Experimental Evolution of the Haloarchaeon

    Kunka, Karina S / Griffith, Jessie M / Holdener, Chase / Bischof, Katarina M / Li, Haofan / DasSarma, Priya / DasSarma, Shiladitya / Slonczewski, Joan L

    Frontiers in microbiology

    2020  Volume 11, Page(s) 535

    Abstract: ... ...

    Abstract Halobacterium
    Language English
    Publishing date 2020-04-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.00535
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Inverted Regulation of Multidrug Efflux Pumps, Acid Resistance, and Porins in Benzoate-Evolved Escherichia coli K-12.

    Moore, Jeremy P / Li, Haofan / Engmann, Morgan L / Bischof, Katarina M / Kunka, Karina S / Harris, Mary E / Tancredi, Anna C / Ditmars, Frederick S / Basting, Preston J / George, Nadja S / Bhagwat, Arvind A / Slonczewski, Joan L

    Applied and environmental microbiology

    2019  Volume 85, Issue 16

    Abstract: Benzoic acid, a partial uncoupler of the proton motive force (PMF), selects for sensitivity to chloramphenicol and tetracycline during the experimental evolution ... ...

    Abstract Benzoic acid, a partial uncoupler of the proton motive force (PMF), selects for sensitivity to chloramphenicol and tetracycline during the experimental evolution of
    MeSH term(s) Anti-Bacterial Agents/metabolism ; Anti-Bacterial Agents/pharmacology ; Benzoates/metabolism ; Benzoates/pharmacology ; Benzoic Acid/metabolism ; Benzoic Acid/pharmacology ; Drug Resistance, Multiple, Bacterial ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Membrane Transport Proteins/genetics ; Membrane Transport Proteins/metabolism ; Porins/genetics ; Porins/metabolism ; Salicylic Acid/metabolism ; Salicylic Acid/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Benzoates ; EmrA protein, E coli ; Escherichia coli Proteins ; MdtE protein, E coli ; MdtF protein, E coli ; Membrane Proteins ; Membrane Transport Proteins ; Porins ; Benzoic Acid (8SKN0B0MIM) ; Salicylic Acid (O414PZ4LPZ)
    Language English
    Publishing date 2019-08-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.00966-19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  6. Article ; Online: Experimental Evolution of Escherichia coli K-12 in the Presence of Proton Motive Force (PMF) Uncoupler Carbonyl Cyanide

    Griffith, Jessie M / Basting, Preston J / Bischof, Katarina M / Wrona, Erintrude P / Kunka, Karina S / Tancredi, Anna C / Moore, Jeremy P / Hyman, Miriam R L / Slonczewski, Joan L

    Applied and environmental microbiology

    2019  Volume 85, Issue 5

    Abstract: Experimental evolution ... ...

    Abstract Experimental evolution of
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology ; Drug Resistance, Bacterial/genetics ; Escherichia coli K12/drug effects ; Escherichia coli K12/genetics ; Escherichia coli Proteins/genetics ; Genes, Bacterial/genetics ; Multidrug Resistance-Associated Proteins/genetics ; Mutation ; Proton-Motive Force ; Repressor Proteins/genetics ; Transcription Factors
    Chemical Substances Anti-Bacterial Agents ; Escherichia coli Proteins ; GadE protein, E coli ; Multidrug Resistance-Associated Proteins ; Repressor Proteins ; Transcription Factors ; mprA protein, E coli ; Carbonyl Cyanide m-Chlorophenyl Hydrazone (555-60-2)
    Language English
    Publishing date 2019-02-20
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.02792-18
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Benzoate- and Salicylate-Tolerant Strains of Escherichia coli K-12 Lose Antibiotic Resistance during Laboratory Evolution.

    Creamer, Kaitlin E / Ditmars, Frederick S / Basting, Preston J / Kunka, Karina S / Hamdallah, Issam N / Bush, Sean P / Scott, Zachary / He, Amanda / Penix, Stephanie R / Gonzales, Alexandra S / Eder, Elizabeth K / Camperchioli, Dominic W / Berndt, Adama / Clark, Michelle W / Rouhier, Kerry A / Slonczewski, Joan L

    Applied and environmental microbiology

    2016  Volume 83, Issue 2

    Abstract: Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic ... ...

    Abstract Escherichia coli K-12 W3110 grows in the presence of membrane-permeant organic acids that can depress cytoplasmic pH and accumulate in the cytoplasm. We conducted experimental evolution by daily diluting cultures in increasing concentrations of benzoic acid (up to 20 mM) buffered at external pH 6.5, a pH at which permeant acids concentrate in the cytoplasm. By 2,000 generations, clones isolated from evolving populations showed increasing tolerance to benzoate but were sensitive to chloramphenicol and tetracycline. Sixteen clones grew to stationary phase in 20 mM benzoate, whereas the ancestral strain W3110 peaked and declined. Similar growth occurred in 10 mM salicylate. Benzoate-evolved strains grew like W3110 in the absence of benzoate, in media buffered at pH 4.8, pH 7.0, or pH 9.0, or in 20 mM acetate or sorbate at pH 6.5. Genomes of 16 strains revealed over 100 mutations, including single-nucleotide polymorphisms (SNPs), large deletions, and insertion knockouts. Most strains acquired deletions in the benzoate-induced multiple antibiotic resistance (Mar) regulon or in associated regulators such as rob and cpxA, as well as the multidrug resistance (MDR) efflux pumps emrA, emrY, and mdtA Strains also lost or downregulated the Gad acid fitness regulon. In 5 mM benzoate or in 2 mM salicylate (2-hydroxybenzoate), most strains showed increased sensitivity to the antibiotics chloramphenicol and tetracycline; some strains were more sensitive than a marA knockout strain. Thus, our benzoate-evolved strains may reveal additional unknown drug resistance components. Benzoate or salicylate selection pressure may cause general loss of MDR genes and regulators.
    Importance: Benzoate is a common food preservative, and salicylate is the primary active metabolite of aspirin. In the gut microbiome, genetic adaptation to salicylate may involve loss or downregulation of inducible multidrug resistance systems. This discovery implies that aspirin therapy may modulate the human gut microbiome to favor salicylate tolerance at the expense of drug resistance. Similar aspirin-associated loss of drug resistance might occur in bacterial pathogens found in arterial plaques.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Anti-Inflammatory Agents, Non-Steroidal/metabolism ; Benzoates/metabolism ; Biological Evolution ; Dose-Response Relationship, Drug ; Drug Resistance, Microbial/genetics ; Escherichia coli K12/drug effects ; Escherichia coli K12/genetics ; Escherichia coli K12/metabolism ; Food Preservatives/metabolism ; Gene Expression Regulation, Bacterial ; Salicylates/metabolism
    Chemical Substances Anti-Bacterial Agents ; Anti-Inflammatory Agents, Non-Steroidal ; Benzoates ; Food Preservatives ; Salicylates
    Language English
    Publishing date 2016-12-30
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.02736-16
    Database MEDical Literature Analysis and Retrieval System OnLINE

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