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  1. Article: Aroma profile and lactic acid bacteria characteristic of traditional Slovak cheese “May bryndza”

    Štefániková, Jana / Árvay, Július / Kunová, Simona / Kowalczewski, Przemysław Łukasz / Kačániová, Miroslava

    Food science and technology international. 2022 Oct., v. 28, no. 7

    2022  

    Abstract: This paper describes the results of the characterization of a traditional Slovak cheese called “May bryndza” with regard to the profiles of volatile organic compounds and lactic acid bacteria. Samples of “May bryndza“ cheese produced solely from ... ...

    Abstract This paper describes the results of the characterization of a traditional Slovak cheese called “May bryndza” with regard to the profiles of volatile organic compounds and lactic acid bacteria. Samples of “May bryndza“ cheese produced solely from unpasteurized ewe's milk were collected from 4 different Slovak farms, and samples of the cheese produced from a mixture of 2 types of milk (raw ewe's and pasteurized cow's milk) were collected from 3 different Slovak industrial dairies. There were 15 compounds detected and identified by the electronic nose. The impact of the kind of milk and the kind of dairy on the aroma profile of the product was not confirmed by PCA. The compounds with the highest relative contents in samples were acetoin (2.59%–24.55%), acetic acid (6.69%–13.39%), methoxy-phenyl-oxime (4.49%–8.52%), butanoic acid (1.89%–5.67%), and 2,3-butanediol (0.98%–4.08%), which were determined with gas chromatography. A total of 1533 isolates of LAB were obtained from the “May bryndza” cheese samples. Four families, five genera, and 19 species were identified with mass spectrometry, and isolated bacteria, both from the farm and industry dairies were the most frequently found to belong to Lactococcus lactis subsp. lactis.
    Keywords Lactococcus lactis subsp. lactis ; acetic acid ; acetoin ; cheeses ; electronic nose ; ewe milk ; ewes ; farms ; gas chromatography ; industry ; lactic acid ; mass spectrometry ; milk ; odors ; pasteurization ; volatile organic compounds
    Language English
    Dates of publication 2022-10
    Size p. 580-591.
    Publishing place SAGE Publications
    Document type Article
    ZDB-ID 1289316-X
    ISSN 1532-1738 ; 1082-0132
    ISSN (online) 1532-1738
    ISSN 1082-0132
    DOI 10.1177/10820132211039916
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Diversity of microbiota in Slovak summer ewes’ cheese “Bryndza”

    Kačániová Miroslava / Terentjeva Margarita / Kunová Simona / Haščík Peter / Kowalczewski Przemysław Łukasz / Štefániková Jana

    Open Life Sciences, Vol 16, Iss 1, Pp 277-

    2021  Volume 286

    Abstract: Bryndza” cheese is an important Slovak traditional regional product. New knowledge on the role of microorganisms involved the “Bryndza” ripening process may provide valuable data on its quality and safety. In our study, the “Bryndza” made from ... ...

    Abstract “Bryndza” cheese is an important Slovak traditional regional product. New knowledge on the role of microorganisms involved the “Bryndza” ripening process may provide valuable data on its quality and safety. In our study, the “Bryndza” made from pasteurized ewes milk was studied towards total count of bacteria, coliforms bacteria, enterococci, lactic acid bacteria, and microscopic filamentous fungi. All those groups of microbiota were detected using classical microbiological methods and identified using mass spectrometry. A total of 3,758 isolates were identified with score higher than 2.00. Altogether, 13 families, 24 genus, and 44 species of microbiota were identified in Slovak cheese “Bryndza.” The most often isolated species were yeasts Yarrowia lipolitica and Dipodascus geotrichum and the lactic acid bacteria Lactobacillus paracasei subsp. paracasei.
    Keywords gram-positive and gram-negative bacteria ; microscopic filamentous fungi ; sheep farms ; ewes milk cheese “bryndza” ; mass spectrometry ; microbiota identification ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher De Gruyter
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Influence of Essential Oils on the Microbiological Quality of Fish Meat during Storage

    Kunová, Simona / Sendra, Esther / Haščík, Peter / Vukovic, Nenad L. / Vukic, Milena / Kačániová, Miroslava

    Animals. 2021 Nov. 04, v. 11, no. 11

    2021  

    Abstract: The aim of the present study was to evaluate the microbiological quality of rainbow trout meat treated with essential oils (EOs from Citrus limon and Cinnamomum camphora) at concentrations of 0.5% and 1.0% in combination with vacuum packaging during ... ...

    Abstract The aim of the present study was to evaluate the microbiological quality of rainbow trout meat treated with essential oils (EOs from Citrus limon and Cinnamomum camphora) at concentrations of 0.5% and 1.0% in combination with vacuum packaging during storage. The composition of the EOs were analyzed by gas chromatography coupled with mass spectrometry, and total viable counts (TVCs), coliform bacteria (CB), and lactic acid bacteria (LAB) were determined on the zeroth, first, third, fifth, and seventh days of storage at 4 °C. Individual species of isolated microorganisms were identified using a MALDI-TOF MS Biotyper. The results show that the major components of the EOs were linalool (98.1%) in C. camphora and α-limonene in C. limon. The highest number of TVCs and CB were 4.49 log CFU/g and 2.65 log CFU/g in aerobically packed samples at the seventh day. The lowest TVCs were those of samples treated with 1% C. camphora EO. For CB the most effective treatment was 1% lemon EO. LAB were only detected in a few samples, and were never present in aerobically packed samples; the highest number of LAB was 1.39 log CFU/g in samples treated with 1% lemon EO at day seven. The most commonly isolated coliform bacteria were Hafnia alvei, Serratia fonticola, Serratia proteamaculans, Pantoea agglomerans, and Yersinia ruckeri. Lactobacillus sakei, Staphylococcus hominis, and Carnobacterium maltaromaticum were the most frequently isolated bacteria from lactic acid bacteria. In conclusion, C. camphora EO at a concentration of 1% showed the highest antimicrobial activity.
    Keywords Carnobacterium piscicola ; Cinnamomum camphora ; Citrus limon ; Hafnia alvei ; Lactobacillus sakei ; Oncorhynchus mykiss ; Pantoea agglomerans ; Serratia fonticola ; Serratia proteamaculans ; Staphylococcus hominis ; Yersinia ruckeri ; antimicrobial properties ; coliform bacteria ; fish meat ; gas chromatography ; lactic acid ; lemons ; meat ; microbiological quality
    Language English
    Dates of publication 2021-1104
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11113145
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Diversity of microbiota in Slovak summer ewes' cheese "Bryndza".

    Kačániová, Miroslava / Terentjeva, Margarita / Kunová, Simona / Haščík, Peter / Kowalczewski, Przemysław Łukasz / Štefániková, Jana

    Open life sciences

    2021  Volume 16, Issue 1, Page(s) 277–286

    Abstract: Bryndza" cheese is an important Slovak traditional regional product. New knowledge on the role of microorganisms involved the "Bryndza" ripening process may provide valuable data on its quality and safety. In our study, the "Bryndza" made from ... ...

    Abstract "Bryndza" cheese is an important Slovak traditional regional product. New knowledge on the role of microorganisms involved the "Bryndza" ripening process may provide valuable data on its quality and safety. In our study, the "Bryndza" made from pasteurized ewes milk was studied towards total count of bacteria, coliforms bacteria, enterococci, lactic acid bacteria, and microscopic filamentous fungi. All those groups of microbiota were detected using classical microbiological methods and identified using mass spectrometry. A total of 3,758 isolates were identified with score higher than 2.00. Altogether, 13 families, 24 genus, and 44 species of microbiota were identified in Slovak cheese "Bryndza." The most often isolated species were yeasts
    Language English
    Publishing date 2021-03-23
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2817958-4
    ISSN 2391-5412 ; 2391-5412
    ISSN (online) 2391-5412
    ISSN 2391-5412
    DOI 10.1515/biol-2021-0038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Influence of Essential Oils on the Microbiological Quality of Fish Meat during Storage.

    Kunová, Simona / Sendra, Esther / Haščík, Peter / Vukovic, Nenad L / Vukic, Milena / Kačániová, Miroslava

    Animals : an open access journal from MDPI

    2021  Volume 11, Issue 11

    Abstract: The aim of the present study was to evaluate the microbiological quality of rainbow trout meat treated with essential oils (EOs ... ...

    Abstract The aim of the present study was to evaluate the microbiological quality of rainbow trout meat treated with essential oils (EOs from
    Language English
    Publishing date 2021-11-04
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11113145
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Aroma profile and lactic acid bacteria characteristic of traditional Slovak cheese "May bryndza".

    Štefániková, Jana / Árvay, Július / Kunová, Simona / Kowalczewski, Przemysław Łukasz / Kačániová, Miroslava

    Food science and technology international = Ciencia y tecnologia de los alimentos internacional

    2021  Volume 28, Issue 7, Page(s) 580–591

    Abstract: This paper describes the results of the characterization of a traditional Slovak cheese called "May bryndza" with regard to the profiles of volatile organic compounds and lactic acid bacteria. Samples of "May bryndza" cheese produced solely from ... ...

    Abstract This paper describes the results of the characterization of a traditional Slovak cheese called "May bryndza" with regard to the profiles of volatile organic compounds and lactic acid bacteria. Samples of "May bryndza" cheese produced solely from unpasteurized ewe's milk were collected from 4 different Slovak farms, and samples of the cheese produced from a mixture of 2 types of milk (raw ewe's and pasteurized cow's milk) were collected from 3 different Slovak industrial dairies. There were 15 compounds detected and identified by the electronic nose. The impact of the kind of milk and the kind of dairy on the aroma profile of the product was not confirmed by PCA. The compounds with the highest relative contents in samples were acetoin (2.59%-24.55%), acetic acid (6.69%-13.39%), methoxy-phenyl-oxime (4.49%-8.52%), butanoic acid (1.89%-5.67%), and 2,3-butanediol (0.98%-4.08%), which were determined with gas chromatography. A total of 1533 isolates of LAB were obtained from the "May bryndza" cheese samples. Four families, five genera, and 19 species were identified with mass spectrometry, and isolated bacteria, both from the farm and industry dairies were the most frequently found to belong to
    MeSH term(s) Acetoin/analysis ; Animals ; Butyric Acid/analysis ; Cattle ; Cheese/analysis ; Female ; Food Microbiology ; Lactobacillales ; Lactococcus lactis ; Milk/microbiology ; Odorants/analysis ; Oximes/analysis ; Sheep ; Slovakia ; Volatile Organic Compounds/analysis
    Chemical Substances Oximes ; Volatile Organic Compounds ; Butyric Acid (107-92-6) ; Acetoin (BG4D34CO2H)
    Language English
    Publishing date 2021-09-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1289316-X
    ISSN 1532-1738 ; 1082-0132
    ISSN (online) 1532-1738
    ISSN 1082-0132
    DOI 10.1177/10820132211039916
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Role of

    Borotová, Petra / Galovičová, Lucia / Vukovic, Nenad L / Vukic, Milena / Kunová, Simona / Hanus, Paweł / Kowalczewski, Przemysław Łukasz / Bakay, Ladislav / Kačániová, Miroslava

    Plants (Basel, Switzerland)

    2022  Volume 11, Issue 11

    Abstract: The essential oil from Litsea cubeba (LCEO) has good antioxidant, antimicrobial, anti-insect properties, which gives it the potential for use as a natural additive to food resources and food products in order to prevent spoilage and extend shelf life. In ...

    Abstract The essential oil from Litsea cubeba (LCEO) has good antioxidant, antimicrobial, anti-insect properties, which gives it the potential for use as a natural additive to food resources and food products in order to prevent spoilage and extend shelf life. In this study the biological activity related to food preservation was observed. The main volatile organic compounds were geranial (39.4%), neral (29.5%), and limonene (14.3%). Antioxidant activity was 30.9%, which was equal to 167.94 µg of Trolox per mL of sample. Antimicrobial activity showed the strongest inhibition against Serratia marcescens by disk diffusion method and minimum inhibitory concentrations MIC 50 and MIC 90 were the lowest for Micrococcus luteus with values 1.46 and 3.52 µL/mL, respectively. Antimicrobial activity of the LCEO vapor phase showed strong inhibition of microorganisms on apples, pears, potatoes, and kohlrabies. Over 50% of gram-positive and gram-negative bacteria and yeasts were inhibited by a concentration of 500 µL/mL. The inhibition of microorganisms was concentration dependent. Anti-insect activity was also strong, with 100% lethality of Pyrrhocoris apterus at a concentration of 25%. These results suggest that LCEO could be potentially used as a food preservative.
    Language English
    Publishing date 2022-06-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants11111504
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Microbiological Quality of Deer Meat Treated with Essential Oil

    Kunová, Simona / Sendra, Esther / Haščík, Peter / Vuković, Nenad L / Vukić, Milena D / Hsouna, Anis Ben / Mnif, Wissem / Kačániová, Miroslava

    Animals : an open access journal from MDPI

    2022  Volume 12, Issue 18

    Abstract: The present study aimed to evaluate deer meat microbiological quality when treated with essential oil (EO) ... ...

    Abstract The present study aimed to evaluate deer meat microbiological quality when treated with essential oil (EO) from
    Language English
    Publishing date 2022-09-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12182315
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Antimicrobial and Antioxidant Activity of Different Honey Samples from Beekeepers and Commercial Producers.

    Kačániová, Miroslava / Borotová, Petra / Galovičová, Lucia / Kunová, Simona / Štefániková, Jana / Kowalczewski, Przemysław Łukasz / Šedík, Peter

    Antibiotics (Basel, Switzerland)

    2022  Volume 11, Issue 9

    Abstract: Honey contains compounds with antioxidant and antibacterial capacities, such as phenolic compounds and carotenoids. The current analysis evaluates the antioxidant and antibacterial activity of 100 honey samples from beekeepers from Slovakia and ... ...

    Abstract Honey contains compounds with antioxidant and antibacterial capacities, such as phenolic compounds and carotenoids. The current analysis evaluates the antioxidant and antibacterial activity of 100 honey samples from beekeepers from Slovakia and commercially purchased ones. Honey samples were diluted to 50%, 25%, 12.5%, and 6.25% concentrations. The antimicrobial activity of honey samples was evaluated against three Gram-positive, three Gram-negative bacteria, and four
    Language English
    Publishing date 2022-08-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics11091163
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Possible Implications of Bacteriospermia on the Sperm Quality, Oxidative Characteristics, and Seminal Cytokine Network in Normozoospermic Men.

    Tvrdá, Eva / Lovíšek, Daniel / Gálová, Eliška / Schwarzová, Marianna / Kováčiková, Eva / Kunová, Simona / Žiarovská, Jana / Kačániová, Miroslava

    International journal of molecular sciences

    2022  Volume 23, Issue 15

    Abstract: This study focused on the identification of bacterial profiles of semen in normozoospermic men and their possible involvement in changes to the sperm structural integrity and functional activity. Furthermore, we studied possible fluctuations of selected ... ...

    Abstract This study focused on the identification of bacterial profiles of semen in normozoospermic men and their possible involvement in changes to the sperm structural integrity and functional activity. Furthermore, we studied possible fluctuations of selected cytokines, oxidative markers, and antibacterial proteins as a result of bacterial presence in the ejaculate. Sperm motility was assessed with computer-assisted sperm analysis, while sperm apoptosis, necrosis and acrosome integrity were examined with fluorescent methods. Reactive oxygen species (ROS) generation was quantified via luminometry, sperm DNA fragmentation was evaluated using the TUNEL protocol and chromatin-dispersion test, while the JC-1 assay was applied to evaluate the mitochondrial membrane potential. Cytokine levels were quantified with the biochip assay, whilst selected antibacterial proteins were quantified using the ELISA method. The predominant species identified by the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry were
    MeSH term(s) Anti-Bacterial Agents/metabolism ; Cytokines/metabolism ; Humans ; Male ; Oxidative Stress ; Reactive Oxygen Species/metabolism ; Semen/metabolism ; Semen Analysis/methods ; Sperm Motility ; Spermatozoa/metabolism
    Chemical Substances Anti-Bacterial Agents ; Cytokines ; Reactive Oxygen Species
    Language English
    Publishing date 2022-08-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23158678
    Database MEDical Literature Analysis and Retrieval System OnLINE

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